Abstract:
:Interneuron precursors transplanted into visual cortex induce network plasticity during their heterochronic maturation. Such plasticity can have a significant impact on the function of the animal and is normally present only during a brief critical period in early postnatal development. Elucidating the synaptic and physiological properties of interneuron precursors as they mature is key to understanding how long-term circuit changes are induced by transplants. We studied the development of transplant-derived interneurons and compared it to endogenously developing interneurons (those that are born and develop in the same animal) at parallel developmental time points, using patch-clamp recordings in acute cortical slices. We found that transplant-derived interneurons develop into fast-spiking and non-fast-spiking neurons characteristic of the medial ganglionic eminence (MGE) lineage. Transplant-derived interneurons matured more rapidly than endogenously developing interneurons, as shown by more hyperpolarized membrane potentials, smaller input resistances, and narrower action potentials at a juvenile age. In addition, transplant-derived fast-spiking interneurons have more quickly saturating input-output relationships and lower maximal firing rates in adulthood, indicating a possible divergence in function. Transplant-derived interneurons both form inhibitory synapses onto host excitatory neurons and receive excitatory synapses from host pyramidal cells. Unitary connection properties are similar to those of host interneurons. These transplant-derived interneurons, however, were less densely functionally connected onto host pyramidal cells than were host interneurons and received fewer spontaneous excitatory inputs from host cells. These findings suggest that many physiological characteristics of interneurons are autonomously determined, while some factors impacting their circuit function may be influenced by the environment in which they develop.NEW & NOTEWORTHY Transplanting embryonic interneurons into older brains induces a period of plasticity in the recipient animal. We find that these interneurons develop typical fast-spiking and non-fast-spiking phenotypes by the end of adolescence. However, the input-output characteristics of transplant-derived neurons diverged from endogenously developing interneurons during adulthood, and they showed lower connection rates to local pyramidal cells at all time points. This suggests a unique and ongoing role of transplant-derived interneurons in host circuits, enabling interneuron transplant therapies.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Larimer P,Spatazza J,Stryker MP,Alvarez-Buylla A,Hasenstaub ARdoi
10.1152/jn.00096.2017subject
Has Abstractpub_date
2017-07-01 00:00:00pages
131-139issue
1eissn
0022-3077issn
1522-1598pii
jn.00096.2017journal_volume
118pub_type
杂志文章abstract::1. Red rod photoreceptors in the toad retina, which are known to be physiologically coupled, were examined for interreceptor contacts. 2. A dense network of large gap junctions was found between the inner segments of red rods, this being the only specialized site of contact that was observed between rods. Each red rod...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1979.42.1.292
更新日期:1979-01-01 00:00:00
abstract::Trigeminal reflex blinks evoked near the onset of a saccade cause profound spatial-temporal perturbations of the saccade that are typically compensated in mid-flight. This paper investigates the influence of reflex blinks on the discharge properties of saccade-related burst neurons (SRBNs) in intermediate and deep lay...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2000.83.6.3430
更新日期:2000-06-01 00:00:00
abstract::Recent studies have shown that the initial responses evoked by a stimulus in neurons of primary visual cortex are dominated by low spatial frequency information in the image, whereas finer spatial scales dominate later in the response. Such phenomena could arise from the dynamics of receptive field (RF) size at early ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00830.2006
更新日期:2007-01-01 00:00:00
abstract::We used positron emission tomography to study motor learning by trial and error. Subjects learned sequences of eight finger movements. Tones generated by a computer told the subjects whether any particular move was correct or incorrect. A control condition was used in which the subjects generated moves, but there was ...
journal_title:Journal of neurophysiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1152/jn.1997.77.3.1325
更新日期:1997-03-01 00:00:00
abstract::We examined the effect of the sweet transduction blocker gurmarin on taste responses recorded from neurons in the rat solitary nucleus (NST) to determine how gurmarin sensitivity is distributed across neuronal type. Initially, responses evoked by washing the anterior tongue and palate with 0.5 M sucrose, 0.1 M NaCl, 0...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00215.2003
更新日期:2003-08-01 00:00:00
abstract::Remembered object locations are stored in an eye-fixed reference frame, so that every time the eyes move, spatial representations must be updated for the arm-motor system to reflect the target's new relative position. To date, studies have not investigated how the brain updates these spatial representations during oth...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.90599.2008
更新日期:2008-11-01 00:00:00
abstract::Despite its widespread use, high-resolution imaging with multiphoton microscopy to record neuronal signals in vivo is limited to the surface of brain tissue because of limited light penetration. Moreover, most imaging studies do not simultaneously record electrical neural activity, which is, however, crucial to unders...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00005.2015
更新日期:2015-06-01 00:00:00
abstract::Controlled whisker stimulation and single-unit recordings were used to elucidate response transformations that occur during the processing of tactile information from ventral posterior medial thalamus (VPM) through cortical columns in the rat whisker/barrel cortex. Whiskers were either deflected alone, using punctate ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.82.4.1808
更新日期:1999-10-01 00:00:00
abstract::1. Single-unit recordings were made, under moderate gaseous anesthesia (33% O2-66% N2O + 0.5/0.6% halothane), in the ventrobasal (VB) thalamic complex of rats (n = 42) with a mononeuropathy created 2-3 wk beforehand, by four loose ligatures around the common sciatic nerve. Before the recording session, three behaviora...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.64.5.1537
更新日期:1990-11-01 00:00:00
abstract::1. Rat dorsal root ganglion (DRG) cell bodies were screened according to action potential (AP) duration, capsaicin sensitivity, expression of IH, IA, and N-, L-, and T-type Ca2+ channel currents. AP duration was measured at half of total amplitude at a membrane potential of -60 mV. Sensitivity to capsaicin was defined...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.5.1870
更新日期:1995-11-01 00:00:00
abstract::An imbalance between the strengths of excitatory and inhibitory synaptic inputs has been proposed as the cellular basis of autism and related neurodevelopmental disorders. Previous studies examining spontaneous levels of excitatory and inhibitory neurotransmission in the forebrain regions of methyl-CpG-binding protein...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00751.2010
更新日期:2011-07-01 00:00:00
abstract::In contrast to hand movements, the existence of a neural representation of saccade kinematics is unclear. Saccade kinematics is typically thought to be specified by motor error/desired displacement and generated by brain stem circuits that are not penetrable to voluntary control. We studied the influence of instructed...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00329.2017
更新日期:2017-09-01 00:00:00
abstract::L-type calcium channels couple membrane depolarization in neurons to numerous processes including gene expression, synaptic efficacy, and cell survival. To establish the contribution of L-type calcium channels to various signaling cascades, investigators have relied on their unique pharmacological sensitivity to dihyd...
journal_title:Journal of neurophysiology
pub_type: 杂志文章,评审
doi:10.1152/jn.00486.2004
更新日期:2004-11-01 00:00:00
abstract::1. Dim backgrounds can enhance small-spot flicker responses of cat retinal horizontal cells by a factor of 2 or more. 2. Intracellular marking with horseradish peroxidase (HRP) reveals that this enhancement effect occurs in--but is not necessarily limited to--the cone-connected, A-type horizontal cell. 3. Flicker ampl...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.64.2.313
更新日期:1990-08-01 00:00:00
abstract::The external anal sphincter (EAS) is important for the maintenance of bowel continence and may be compromised by a variety of neuropathic conditions. However, large animal models for the study of EAS functions have been sparse. The EAS guarding reflex was examined by electromyography (EMG) in neurologically intact rhe...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00435.2017
更新日期:2018-12-01 00:00:00
abstract::Facial flushing in rosacea is often induced by trigger events. However, trigger causation mechanisms are currently unclear. This study tested the central hypothesis that rosacea causes sympathetic and axon reflex-mediated alterations resulting in trigger-induced symptomatology. Twenty rosacea patients and age/sex-matc...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00458.2015
更新日期:2015-09-01 00:00:00
abstract::1. The modulation of the voltage-activated Ca2+ current by the neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFa) was investigated in dissociated central neurons from Helix aspersa using whole-cell voltage-clamp recording techniques. External Ba2+ was always used as the charge carrier in this study, and the intracellular Ca2+ c...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1991.65.6.1517
更新日期:1991-06-01 00:00:00
abstract::Despite the importance of the thumb to hand function, little is known about the origins of thumb impairment poststroke. Accordingly, the primary purpose of this study was to assess whether thumb flexors have heightened stretch reflexes (SRs) following stroke-induced hand impairment. The secondary purpose was to compar...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00423.2009
更新日期:2010-10-01 00:00:00
abstract::EEG generator-a model of potentials in a volume conductor. The potential recorded over the cortex electro-corticogram (ECoG) or over the scalp [electroencephalograph (EEG)] derives from the activity of many sources known as "EEG generators." The recorded amplitude is basically a function of the unitary potential of a ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.91143.2008
更新日期:2009-11-01 00:00:00
abstract::1. Three parallel maps of the distribution of tuberous electroreceptor inputs are found in the medullary electrosensory lateral line lobe (ELL) of weakly electric fish. Pyramidal cells in each map are known to respond differentially to the frequency of amplitude modulations (AMs) of external electric fields in vivo. W...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.4.2364
更新日期:1996-10-01 00:00:00
abstract::Bis(7)-tacrine is a potent acetylcholinesterase inhibitor in which two tacrine molecules are linked by a heptylene chain. We tested the effects of bis(7)-tacrine on the spontaneous synaptic activity. Miniature endplate potentials (MEPPs) were recorded extracellularly on slices of electric organ of Torpedo marmorata. B...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.86.1.183
更新日期:2001-07-01 00:00:00
abstract::Selective attention operates in visual cortex by facilitating processing of selected stimuli and by filtering out unwanted information from nearby distracters over circumscribed regions of visual space. The neural representation of unattended stimuli outside this focus of attention is less well understood. We studied ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00974.2003
更新日期:2004-07-01 00:00:00
abstract::Manipulating a cup by the handle requires compensating for the torque induced by the moment of the mass of the cup relative to the location of the handle. In the present study, we investigated the control strategy of subjects asked to perform grip-lift movements with an object with center of mass located away from the...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00367.2011
更新日期:2011-12-01 00:00:00
abstract::The centre médian-parafascicular (CM-Pf) complex is located at the posterior intralaminar nuclei of the thalamus and forms part of the nonspecific thalamocortical projection system and the internal circuit of the basal ganglia. However, the functional roles of this complex remain to be fully elucidated. Here we have e...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2002.87.6.3090
更新日期:2002-06-01 00:00:00
abstract::The role of potassium channels in regulating spontaneous firing and sound-evoked responses in the central nucleus of the inferior colliculus was studied by recording single-unit activity before and during iontophoretic application of a nonspecific potassium channel blocker, tetraethylammonium (TEA). Tone bursts and si...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00730.2003
更新日期:2004-05-01 00:00:00
abstract::The corticotectal influences of somatosensory cortex were investigated by using reversible deactivation of cortex by cooling. More than half of the somatosensory superior colliculus (SC) cells studied exhibited a response depression (often not apparent qualitatively) or an elimination of responses to somatosensory sti...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1986.55.6.1352
更新日期:1986-06-01 00:00:00
abstract::An increase in L-arginine hemolymph concentration acts as a postingestion signal inhibiting Aplysia feeding. At physiological concentrations (a 10-μM increase over background), the inhibitory effect of L-arginine is too weak to block feeding in hungry animals. However, a 10-μM increase in L-arginine concentration acts...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00827.2010
更新日期:2011-04-01 00:00:00
abstract::Our group set out to develop a sensitive technique, capable of detecting output changes from the posterior fossa following a motor acquisition task. Transcranial magnetic stimulation (TMS) was applied over the right cerebellar cortex 5 ms in advance of test stimuli over the left cerebral motor cortex (M1), suppressing...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00661.2013
更新日期:2014-02-01 00:00:00
abstract::Regulation of feeding behavior involves the integration of multiple physiological and neurological pathways that control both nutrient-seeking and consummatory behaviors. The consummatory phase of ingestion includes stereotyped oromotor movements of the tongue and jaw that are controlled through brain stem pathways. T...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00091.2014
更新日期:2014-08-01 00:00:00
abstract::Single neurons were recorded from the ventral posteromedial thalamic nucleus (VPM) of urethan-anesthetized rats. Six of these animals were intact, 28 sustained kainic acid (KA) lesions of trigeminal nucleus principalis (PrV), and 9 received similar lesions of trigeminal subnucleus interpolaris (SpVi). Four animals sus...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.5.1577
更新日期:1987-05-01 00:00:00