Effect of Gamma Rays on Sophora davidii and Detection of DNA Polymorphism through ISSR Marker.

Abstract:

:Sophora davidii (Franch.) Kom. ex Pavol is an important medicinal plant and a feeding scrub with ecological value. The effects of different gamma irradiation doses (20-140 Kr) on seed germination and seedling morphology were investigated in S. davidii, and intersimple sequence repeat (ISSR) markers were used to identify the DNA polymorphism among mutants. Significant variations were observed for seed germination, stem diameter, and number of branches per plant. The improved agronomic traits, such as stem diameter and number of branches per plant, were recorded at 80 Kr dose and 20 Kr dose for seed germination. ISSR analysis generated in total 183 scorable fragments, of which 94 (51.37%) were polymorphic. The percentage of polymorphism ranged from 14.29 to 93.33 with an average of 45.69%. Jaccard's coefficients of dissimilarity varied from 0.6885 to 1.000, indicative of the level of genetic variation among the mutants. The constructed dendrogram grouped the entities into five clusters. Consequently, it was concluded that gamma rays irradiation of seeds generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker for the identification of mutants.

journal_name

Biomed Res Int

authors

Wang P,Zhang Y,Zhao L,Mo B,Luo T

doi

10.1155/2017/8576404

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

8576404

eissn

2314-6133

issn

2314-6141

journal_volume

2017

pub_type

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