Abstract:
:Resveratrol has been reported to inhibit or induce DNA damage, depending upon the type of cell and the experimental conditions. Dietary resveratrol is present in the body predominantly as metabolites and limited data is available concerning the activities of these metabolic products. In the present study, physiologically obtainable levels of the resveratrol metabolites resveratrol-3-O-glucuronide, resveratrol-4'-O-glucuronide and resveratrol-3-O-sulfate were evaluated for their ability to protect Jurkat T cells against DNA damage induced by the topoisomerase I inhibitors camptothecin and topotecan. The cells were pretreated for 24 h with 10 µM resveratrol aglycone or each resveratrol metabolite prior to the induction of DNA damage with camptothecin or topotecan. In separate experiments, the cells were co-treated with resveratrol or its metabolites, and a topoisomerase I inhibitor. The detection of histone 2AX phosphorylation and terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) were used to determine DNA damage, and apoptosis was measured using an antibody against cleaved poly ADP-ribose polymerase. It was identified that pretreatment of the cells with resveratrol-3-O-glucuronide and resveratrol-4'-O-glucuronide reduced the mean fluorescence intensity of staining for DNA strand breaks following treatment with camptothecin, while the percentage of cells undergoing apoptosis was unchanged. However, pretreatment of the cells with resveratrol aglycone increased the DNA damage and apoptosis induced by the drugs. These results suggest that the glucuronide metabolites of resveratrol partially protected the cells from DNA damage, but did not influence the induction of cell death by camptothecin and topotecan. These data suggest that resveratrol aglycone treatment may be beneficial for treating types of cancer that have direct contact with resveratrol prior to its metabolism, including gastrointestinal cancers, which are routinely treated with topoisomerase I inhibitors.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Zunino SJ,Storms DHdoi
10.3892/ol.2017.6392subject
Has Abstractpub_date
2017-08-01 00:00:00pages
2517-2522issue
2eissn
1792-1074issn
1792-1082pii
OL-0-0-6392journal_volume
14pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::MicroRNAs (miRs) are an emerging class of non-coding, endogenous and small RNA molecules that serve important functions in tumorigenesis and development. The present study investigated the expression, functions and molecular mechanism underlying miR-205 in hepatocellular carcinoma. miR-205 was downregulated in hepatoc...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8933
更新日期:2018-08-01 00:00:00
abstract::The present study aimed to assess the feasibility, safety and efficiency of ultrasound-guided percutaneous microwave ablation (MWA) on liver metastases from colon or rectal cancer. Patients who received MWA therapy for liver metastases from colon or rectal cancer between June 2009 and May 2012 were enrolled in the stu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2106
更新日期:2014-07-01 00:00:00
abstract::Gliomas are the most common type of primary brain tumor in adults, where more than half of the cases are malignant, and the prognosis is poor. The early viral 1A (E1A) protein has been widely recognized to be essential for adenoviral replication and production of progeny virions in human cells, a process that is regul...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7684
更新日期:2018-03-01 00:00:00
abstract::Leptomeningeal metastasis (LM) is an uncommon presentation of relapse in breast cancer, which is associated with poor clinical outcomes and poor prognosis. Notably, LM most commonly occurs in breast cancer. The aim of the present review was to investigate the occurrence of LM as the primary presentation of relapse fol...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4745
更新日期:2016-08-01 00:00:00
abstract::The correlation between expression and prognosis of thyroid transcription factor-1 (TTF-1) and CD44v6 in undifferentiated lung carcinoma was investigated. There were 116 cases with large cell undifferentiated carcinoma in group A, 120 cases with small cell undifferentiated carcinoma in group B and 80 normal individual...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10689
更新日期:2019-10-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) contributes to 14.8% of all cancer mortality in Egypt, which has a high prevalence of hepatitis C virus (HCV). We have previously shown alterations in the insulin-like growth factor-1 (IGF-1) receptor signalling pathway during experimental hepatocarcinogenesis. The aim of this study was ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.546
更新日期:2012-03-01 00:00:00
abstract::The present study aimed to investigate the effect of microRNA-150 (miRNA/miR-150) in osteosarcoma (OS) cell invasion and metastasis by the regulation of Ezrin. To compare the differences in the expression of miR-150 and Ezrin, cell models of OS metastasis were established by exogenous transfection of miR-150 on the ba...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5046
更新日期:2016-11-01 00:00:00
abstract::Papillary thyroid cancer (PTC) is the most common type of thyroid malignancy, and it is often observed to overexpress epidermal growth factor receptor (EGFR). Previous research has indicated that EH domain-containing 1 (EHD1) is associated with EGFR-mediated endocytotic recycling in multiple tumor types. The objective...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9200
更新日期:2018-10-01 00:00:00
abstract::The aim of the present study was to detect the amplification of the human epidermal growth factor receptor 2 (HER2) gene in esophageal squamous cell carcinoma (ESCC), gastroesophageal junction adenocarcinoma (GEJAC) and gastric cancer (GC), as well as to understand the pathological meaning of HER2 gene amplification w...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1348
更新日期:2013-07-01 00:00:00
abstract::Effects of different levels of PM2.5 on invasion and proliferation of HeLa cells and the expression levels of inflammatory cytokines IL-1 and IL-6 under 10 µg/ml PM2.5 were investigated. Different groups of HeLa cells were treated with PM2.5 at 0, 10, 25, 50, 100 and 200 µg/ml for 36 h, respectively. Cell proliferatio...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9516
更新日期:2018-12-01 00:00:00
abstract::The present study examined the expression of podocalyxin (PODX) in surgically-resected astrocytomas, associated the levels of PODX expression with the clinicopathological characteristics and survival outcomes of astrocytoma and assessed how PODX affected the viability of astrocytoma cells following the administration ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1556
更新日期:2013-11-01 00:00:00
abstract::Ovarian cancer (OC) has the worst prognosis among all malignancy types in females worldwide according to epidemiological studies in 2017. Although radiotherapy, chemotherapy and surgical treatment are the most common treatment methods, their curative effects are not satisfactory. The present study aimed to examine the...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10306
更新日期:2019-07-01 00:00:00
abstract::Protein tyrosine phosphatase receptor type A (PTPRA), one of the classic protein tyrosine phosphatases, is crucial for modulating tumorigenesis and metastasis in breast cancer; however, its functional mechanism has not fully elucidated. The present study assessed PTPRA expression and estimated its clinical impact on s...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11992
更新日期:2020-11-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) have been reported to participate in multiple biological processes, including tumorigenesis. In the current study, the function of a novel lncRNA LINC00887 was investigated in lung carcinoma. For this purpose, LINC00887 expression was assessed by reverse-transcription quantitative PCR. C...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12348
更新日期:2021-02-01 00:00:00
abstract::Being biomarkers that reflect host nutritional and immune status, prognostic nutritional index (PNI) and neutrophil/lymphocyte ratio (NLR) have been identified to be independent prognostic factors in various malignancies. The aim of the present study was to determine the predictive value of these parameters for the pr...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9870
更新日期:2019-02-01 00:00:00
abstract::Interleukin-8 (IL-8), an important inflammatory factor, is induced by Helicobacter pylori infection and is clearly associated with gastric cancer. IL-8 levels have been revealed to correlate significantly with the adhesion, migration and invasion of gastric cancer cells. However, whether IL-8 influences cell prolifera...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2531
更新日期:2014-12-01 00:00:00
abstract::Colorectal carcinoma (CRC) is a common tumor of the digestive system with poor prognosis. Studies have shown that aberrant microRNA (miRNA) expression can affect CRC progression by regulating target genes. In the present study, we investigated the functional roles and potential mechanisms of miR-331-3p in CRC. The exp...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11029
更新日期:2019-12-01 00:00:00
abstract::The aim of the present study was to investigate the effect of HPV16E6 gene integration on the biological behavior of Eca109 and Eca9706 cells. This was evaluated through positive liposome transfection of HPV16E6 into Eca109 cells and Eca9706 cells. The transfection efficiency was evaluated by calculating the ratio of ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7469
更新日期:2018-02-01 00:00:00
abstract::Clear cell renal cell carcinoma (ccRCC) exhibits the highest mortality among all urological malignancies. The investigation of the potential disease progression markers can improve ccRCC diagnosis and treatment. Gene associated with retinoid-interferon-induced mortality-19 (GRIM-19) is involved in carcinogenesis and c...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11498
更新日期:2020-06-01 00:00:00
abstract::For individualized bevacizumab-based therapy, non-invasive biomarkers are necessary. This study assessed the predictive value of plasma vascular endothelial growth factor (VEGF)-A, soluble VEGF receptor (sVEGFR)-1 and sVEGFR-2 levels as biomarkers for clinical response and survival in advanced colorectal cancer (CRC) ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol_00000045
更新日期:2010-03-01 00:00:00
abstract::This study investigated the mRNA levels of thymidylate synthetase (TYMS) and dihydropyrimidine dehydrogenase (DPYD) in esophageal squamous cell carcinoma (ESCC). TYMS and DPYD gene expression was quantified using real-time RT-PCR in 56 patients with ESCC, co-amplified with glyceraldehyde-3-phosphate dehydrogenase as a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2010.227
更新日期:2011-03-01 00:00:00
abstract::The present study compared two methods for the detection of severe cervical dysplasia in women with atypical squamous cells of underdetermined significance (ASC-US) cytology; digital colposcopy with adjunctive dynamic spectral imaging (DSI) and conventional colposcopy. IMPROVE-COLPO was a two-arm cross-sectional study...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11878
更新日期:2020-10-01 00:00:00
abstract::The present study aimed to evaluate the expression of hypoxia-inducible factor-1α (HIF-1α) and MDR1/P-glycoprotein (P-gp) in human laryngeal squamous cell carcinoma (LSCC) tissues, and also to investigate the regulation of MDR1 gene expression by HIF-1α in Hep-2 cells under hypoxic conditions. The expression of HIF-1α...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1321
更新日期:2013-07-01 00:00:00
abstract::The aim of the present study was to evaluate the clinical value of magnetic resonance imaging (MRI) in the preoperative T staging of gastric cancer and in the postoperative pathological diagnosis. In total, 30 patients with gastric cancer were investigated, including 19 males and 11 females (age, 50-69 years; mean age...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2135
更新日期:2014-07-01 00:00:00
abstract::The aim of the study was to determine whether interleukin-8 (IL-8) affects human SKOV3 ovarian cancer cell migration and invasion by targeting silencing of IL-8 expression. Silencing small-interfering RNA (siRNA) targeting IL-8 gene was constructed to infect SKOV3 cells by lentiviral vector. The expression of IL-8 and...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5511
更新日期:2017-02-01 00:00:00
abstract::The endogenous lipid metabolism network is associated with the occurrence and progression of malignancies. Endocannabinoids and ceramides have demonstrated their anti-proliferative and pro-apoptotic properties in a series of cancer studies. The aim of the present study was to evaluate the expression patterns of endoca...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10399
更新日期:2019-08-01 00:00:00
abstract::The aim of this study was to objectively evaluate the benefits of laparoscopically assisted vaginal radical hysterectomy and lymphadenectomy for early-stage cervical cancer. Clinical data were prospectively collected from patients with IA-IIB cervical cancer who underwent laparoscopically assisted vaginal radical hyst...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.311
更新日期:2011-07-01 00:00:00
abstract::The present study aimed to examine whether monoclonal immunoglobulin heavy chain (IGH) or T-cell receptor (TCR) gene rearrangement in cell-free DNA (cfDNA) may be used for minimal residual disease (MRD) monitoring in patients with acute myeloid leukemia (AML). Monoclonal IGH and TCR rearrangement in cfDNA were monitor...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8966
更新日期:2018-08-01 00:00:00
abstract::The clinical decisions made when treating patients with metastatic cancer require knowledge of the current tumor extent and response to therapy. For the majority of solid tumors, a response assessment, which is based on imaging, is used to guide these decisions. However, measuring serum protein biomarkers (i.e. tumor ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11192
更新日期:2020-02-01 00:00:00
abstract::Change in mitochondrial DNA (mtDNA) copy number has been reported in esophageal squamous cell carcinoma (ESCC). However, its prognostic implication in ESCC remains largely unknown. Using reverse transcription-quantitative PCR assay, the mtDNA copy number was assessed in a cohort of patients with ESCC (n=141) and norma...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7416
更新日期:2018-01-01 00:00:00