Abstract:
:In case of a bite by a rabies infected animal, the World Health Organisation recommends a prophylactic treatment including the administration of Human Rabies Immunoglobulins (HRIGs) or highly purified F(ab')2 fragments produced from Equine Rabies Immunoglobulin (F(ab')2 - ERIGs). According to international regulation, quality control of F(ab')2 - ERIGs lots requires potency testing by the in vivo Mouse Neutralisation Test (MNT) prior marketing. However, the strategy of the 3Rs (Reduce, Refine, Replace) for animal testing required by the European Directive encourages the replacement of the in vivo potency test by an in vitro assay. In this context, a competitive ELISA method (c-ELISA) has been developed by the Agence Nationale de Sécurité du Médicament et des Produits de Santé where F(ab')2 - ERIGs are in competition with a monoclonal antibody recognizing the trimeric native form of the rabies glycoprotein. After a full validation study, the c-ELISA has been applied to commercial batches of F(ab')2 - ERIGs. A correlation study with the MNT demonstrated a similarity between the two methods (r=0.751). Moreover, the c-ELISA method which does not need any species specific reagent has been applied to HRIGs potency testing as an alternative method to Rapid Fluorescent Focus Inhibition Test (RFFIT), thus avoiding the handling of live rabies virus in BSL3 containment. In conclusion, the c-ELISA has shown its potential to replace MNT and possibly RFFIT for the quantification of rabies immunoglobulin. After optimisation it may be used for the quantification of rabies immunoglobulin in any animal species, notably for rabies immunogenicity assay in mice.
journal_name
Vaccinejournal_title
Vaccineauthors
Korimbocus J,Dehay N,Tordo N,Cano F,Morgeaux Sdoi
10.1016/j.vaccine.2016.04.086subject
Has Abstractpub_date
2016-06-14 00:00:00pages
3310-6issue
28eissn
0264-410Xissn
1873-2518pii
S0264-410X(16)30261-4journal_volume
34pub_type
杂志文章相关文献
VACCINE文献大全abstract::Immunisation is one of the corner stones of public health. Most health care consumers see the health care worker as their major source of information on immunisation and vaccine safety. Doctors, nurses and midwives should be appropriately and timely trained for that role. Within the Vaccine Safety, Attitudes, Training...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.12.033
更新日期:2010-02-25 00:00:00
abstract::It has been shown recently that BCG can be used as a live recombinant vaccine to stimulate immune responses. Proliferative or cytotoxic T-cell responses against several viral proteins such as HIV Gag, Env or Nef were obtained after parenteral immunization with BCG expressing these proteins. Antibody responses were als...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90096-g
更新日期:1993-10-01 00:00:00
abstract::Glycoconjugate vaccines play an enormous role in preventing infectious diseases. The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT). Modern childhood routine vaccination schedules include the administrati...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.11.026
更新日期:2015-01-03 00:00:00
abstract::Vibrio cholerae O139 Bengal strain was the causative agent of the recent epidemics of cholera in India and Bangladesh. We studied antibacterial and antitoxin immune responses in acute and convalescent phase paired sera collected from seven of these cholera patients. Significant rise in the levels of both antibacterial...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(96)00035-7
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:In 2014 the Kenya National Immunization Technical Advisory Group (KENITAG) was asked by the Ministry of Health to provide an evidence-based recommendation on whether the seasonal influenza vaccine should be introduced into the national immunization program (NIP). METHODS:We reviewed KENITAG manuals, reports...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.11.062
更新日期:2019-01-14 00:00:00
abstract::The innocuousness of the Brucella melitensis Rev 1 live attenuated vaccine strain has never been fully assessed in rams. The immunopathological responses and the kinetics and distribution of the infection induced by this strain were determined after subcutaneous or conjunctival vaccination in both young (3-4 months ol...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.03.030
更新日期:2008-05-19 00:00:00
abstract::Viral hepatitis remains a major contributor to the global disease burden. Mass immunisation strategies against hepatitis B have been adopted by more than 90 developing and industrialised countries. Countries with low hepatitis A endemicity are experiencing cyclical outbreaks and an epidemiological shift, with larger n...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00193-6
更新日期:2000-10-15 00:00:00
abstract::Trehalose-6,6'-dimycolate (TDM) and its monosaccharide-type analogues were synthesized, and their lethal and adjuvant activities were examined in mice. All the monosaccharide-type analogues with a glucose or N-acetylglucosamine moiety were devoid of lethal toxicity to mice; in particular, D-GlcNAcM(1-deoxy) and D-GlcN...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(89)90242-9
更新日期:1989-06-01 00:00:00
abstract::Household surveys are frequently used as means of vaccination coverage measurement, but obtaining accurate survey estimates present several challenges. In 2015, the World Health Organization (WHO) released a working draft of its updated Vaccination Coverage Survey Reference Manual that moved well beyond the traditiona...
journal_title:Vaccine
pub_type:
doi:10.1016/j.vaccine.2018.07.019
更新日期:2018-08-16 00:00:00
abstract:BACKGROUND:MenB-4C (Bexsero®) is a multicomponent serogroup B meningococcal vaccine. For vaccine licensure, efficacy was inferred from serum bactericidal antibody (SBA) against three antigen-specific indicator strains. The bactericidal role of antibody to the fourth vaccine antigen, Neisserial Heparin binding antigen (...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.06.020
更新日期:2017-07-24 00:00:00
abstract::The HIV epidemic in the south Indian state of Karnataka disproportionately burdens key populations of men who have sex with men and female sex workers. Despite having successfully reduced HIV incidence among certain key populations through the use of targeted intervention, India's HIV epidemic remains one of its great...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.12.009
更新日期:2015-01-29 00:00:00
abstract::Neutralizing antibodies play an essential part in antiviral immunity and are instrumental in preventing or modulating viral diseases. Polyclonal antibody preparations are increasingly being replaced by highly potent monoclonal antibodies (mAbs). Cocktails of mAbs and bispecific constructs can be used to simultaneously...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2013.01.025
更新日期:2013-03-15 00:00:00
abstract::Gavi, the Vaccine Alliance, supports immunisation programmes in eligible countries to reach children with lifesaving vaccines. Dramatic improvement in the scale and performance of current cold chain systems is required to extend the reach of immunisation services - especially for children living in remote locations - ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2016.12.073
更新日期:2017-04-19 00:00:00
abstract::Haemagglutinin (HA), a mixture of haemagglutinin and neuraminidase (HA + NA), and matrix (M) protein were isolated from the influenza A virus and covalently coupled to a synthetic polyelectrolyte (P). A single injection into mice of the resultant conjugates (virogates) brought about efficient stimulation of the primar...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(85)90130-6
更新日期:1985-12-01 00:00:00
abstract:INTRODUCTION:HPV vaccine uptake is lowest among young adults. Little is known about the most effective way to decrease missed opportunities (MO) and increase uptake of the vaccine in this vulnerable population. OBJECTIVES:To determine the impact of a clinical intervention bundle on the rate of MO and uptake of the vac...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.05.039
更新日期:2018-06-14 00:00:00
abstract::Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent immune stimulant when administered with different vaccines. Optimal use of GM-CSF resides in its ability to act locally to stimulate the proliferation and maturation of professional antigen-presenting cells (APCs) (i.e., Langerhans' cells) at the in...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.11.060
更新日期:2005-04-22 00:00:00
abstract::Recombinant canarypox (CP) and fowlpox (FP) viruses that contained two forms of the HIV-1 (SF2 strain) env gene were engineered and their expression analysed in chick, simian and human cells. These vectors can efficiently replicate in avian but not in mammalian cells, in which infection is abortive. The two forms, con...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90180-5
更新日期:1994-09-01 00:00:00
abstract::To test several vaccines for Chlamydia trachomatis we vaccinated BALB/c and C3H/HeN female mice with a purified preparation of the native mouse pneumonitis (MoPn) major outer membrane protein (MOMP). The MOMP was formulated with anyone of three different adjuvants MF59, LT-K63 or LT-R72. As a negative control the anim...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.08.074
更新日期:2006-02-06 00:00:00
abstract::Shigellosis is a major form of bacillary dysentery caused by Shigella infection. Shigella ribosome-based vaccines (SRV), considered among the potent vaccine candidates, are composed of O-antigen and ribosome isolated from S. flexneri 2a. To investigate the immunogenicity and protective efficacy of SRV, mice were vacci...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.03.050
更新日期:2007-06-15 00:00:00
abstract::Influenza A viruses pose a substantial threat to the human population whether by purposeful manipulation and release or by the natural process of interspecies transmissions from animal reservoirs. The challenge with preparing for these events with vaccination strategies is that the best forms of protective immunity ta...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2009.08.038
更新日期:2009-11-05 00:00:00
abstract::Activation of the innate immune system by different homotopes may direct the subsequent specific immune response, and as a consequence may have significant implications on vaccination. In this study, effects of i.v. administered lipopolysaccharide (LPS), and lipoteichoic acid (LTA), respectively, and s.c. administered...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.11.037
更新日期:2005-04-15 00:00:00
abstract::Bacterial infectious diseases remain the single most important threat to health worldwide. Although conventional vaccinology approaches were successful in conferring protection against several diseases, they failed to provide efficacious solutions against many others. The advent of whole-genome sequencing changed the ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2009.01.072
更新日期:2009-05-26 00:00:00
abstract::Pregnant women and their infants are vulnerable to severe disease and secondary complications from influenza infection. For this reason, annual influenza vaccination is recommended for all pregnant women in the United States. Women frequently cite concerns about vaccine safety as a barrier to vaccination. This review ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2014.04.021
更新日期:2014-05-30 00:00:00
abstract::Measles incidence has decreased dramatically in China due to the implement of measles-containing vaccine (MCV). However, a measles epidemic caused resurgence recently, even among vaccinated individuals. To evaluate the effectiveness of current immunization programs and discuss initiatives for the next step in measles ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.03.049
更新日期:2020-05-08 00:00:00
abstract::In this study, in vivo electroporation of a DNA vaccine adjuvanted with plasmids encoding different cytokines was investigated in large animals. Sheep were injected intramuscularly with a DNA vaccine encoding an antigen of Haemonchus contortus (pNPA) and plasmids encoding different cytokines followed by in vivo electr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.12.025
更新日期:2007-03-30 00:00:00
abstract:BACKGROUND:In the Netherlands, a relatively low varicella disease burden compared to other European countries is observed within routine surveillance. To validate this, we estimated the varicella-related consultation rate using The Integrated Primary Care Information database. METHODS:In this retrospective cohort stud...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.04.034
更新日期:2014-06-12 00:00:00
abstract::As measured by fluid accumulation in ileal loops, Vibrio cholerae mucinase complex, with or without toxoid, protected guinea pigs from challenges with V. cholerae live organisms and enterotoxin. The neuraminidase and proteinases of the complex were combined in modified oil emulsion or aluminum hydroxide adjuvants and ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.12.004
更新日期:2004-06-02 00:00:00
abstract::This study assessed (1) T-cell responses following vaccination of HIV-1 negative volunteers with HIV-1LAI recombinant gp160 (rgp160) vaccine, and (2) effects of vaccine injection schedule and MHC HLA type on those responses. In one trial, volunteers received rgp160 in two accelerated schedules (Groups 1 and 2). In the...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(95)00020-2
更新日期:1995-09-01 00:00:00
abstract:BACKGROUND:Shigellae cause severe disease in endemic countries, especially in children. Several efficacy trials have been conducted with candidate vaccines against Shigellae, but the lack of protection, the safety concerns, or manufacturing challenges hindered successful market approval. Conjugated vaccines have been s...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2015.06.102
更新日期:2015-08-26 00:00:00
abstract:AIM:Design and immunogenicity assessment of the combined vaccine candidate against zoonotic hepatitis E virus (HEV) and foot-and-mouth disease virus (FMDV). METHODS:Using the molecular cloning technology, we produced and purified 9 HEV ORF2-truncated proteins (HEV genotype 4). Then, we compared their thermal stability...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.09.036
更新日期:2019-10-31 00:00:00