Thioredoxin 1 is associated with the proliferation and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes.

Abstract:

:We aimed to investigate the possible effects of thioredoxin 1 (Trx1) on the proliferation and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) and elucidate the possible mechanisms involved. We investigated the distribution and expression of Trx1 in synovial tissues from RA and osteoarthritis (OA) patients by immunohistochemistry and real-time polymerase chain reaction (RT-PCR) analyses. RA-FLSs were isolated and cultured under normoxic (21% oxygen) or hypoxic (3% oxygen) concentrations. Transfection of Trx1-siRNAs and a Trx1 overexpression construct was conducted to manipulate the expression of Trx1. Protein expression was detected by Western blot. Doxorubicin (Adriamycin, ADR) was used to induce apoptosis. LY-294002 was used for the inhibition of PI3K-Akt. Cell proliferation and apoptosis were determined by MTS (3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium, inner salt) assay and flow cytometry, respectively. The mRNA and protein expression of Trx1 in RA tissues was higher than that in OA tissues. The expression levels of Trx1 and cell proliferation in RA-FLSs were increased under hypoxia in comparison to those under normoxia. In hypoxia, downregulation of Trx1 significantly suppressed FLS proliferation, and the expression of PI3Kp85, phospho-Akt, and Bcl-2, while notably increased FLS apoptosis and the expression of active Caspase3 and Bax. In normoxia, Trx1 overexpression promoted the FLS proliferation and the expression of PI3Kp85, phospho-Akt, and Bcl-2, but inhibited FLS apoptosis and the expression of active Caspase3 and Bax in FLSs. Such effects were partially repressed by LY-294002 treatment. Trx1 may play an important role in regulating the proliferation and apoptosis of RA-FLSs by modulating PI3K-Akt activation.

journal_name

Clin Rheumatol

journal_title

Clinical rheumatology

authors

Lu T,Zong M,Fan S,Lu Y,Yu S,Fan L

doi

10.1007/s10067-017-3832-1

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

117-125

issue

1

eissn

0770-3198

issn

1434-9949

pii

10.1007/s10067-017-3832-1

journal_volume

37

pub_type

杂志文章
  • A case of Sjögren's syndrome complicating immune-mediated aplastic anaemia.

    abstract::A 78-year-old Japanese woman with Sjögren's syndrome complicating immune-mediated aplastic anaemia is described. A diagnosis of aplastic anaemia was made from severe pancytopenia with hypoplastic marrow. Laboratory studies suggested an association of bone marrow suppressive T-lymphocytes with the pathogenesis of aplas...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/BF02231538

    authors: Satoh M,Yamagata H,Watanabe F,Matsushita Y,Nakayama S,Murakami M,Matsuyama J,Oshima S,Akizuki M

    更新日期:1993-06-01 00:00:00

  • The risk of leukemia in patients with rheumatoid arthritis: a systematic review and meta-analysis.

    abstract:OBJECTIVES:The relationship between rheumatoid arthritis (RA) and the risk of leukemia was still controversial. This study aimed to assess the risk of leukemia in patients with rheumatoid arthritis by systematic review and meta-analysis. METHODS:Relevant studies were identified by searching PubMed, Embase, Cochrane Li...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-020-05396-7

    authors: Luo X,He Y,Xu W,Liu M,Zhao Z,Peng L,He C,Chen J

    更新日期:2020-09-17 00:00:00

  • Charcot joint in idiopathic sensorimotor neuropathy.

    abstract::Charcot joint or neuropathic arthropathy is described in certain neurological conditions. We report the case of a man who presented with a swollen ankle 10 days after a long walk, which rapidly progressed to a Charcot joint. A neurological examination revealed areflexia and insensitivity to temperature and pain. Elect...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s100670050036

    authors: Chappel R,Willems J,Martin JJ

    更新日期:2000-01-01 00:00:00

  • Intracellular signal transduction in proliferation of synovial cells.

    abstract::We investigated some steps in the signal transduction pathway leading to the proliferation of synovial cells. 12-o-tetradecanoyl phorbol-13-acetate (TPA) which is known to stimulate phospholipid- and Ca(2+)-dependent protein kinase (C-kinase) enhanced the proliferation of synovial cells. The proliferation of synovial ...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/BF02207092

    authors: Yamamoto M,Yasuda M,Shiokawa S,Nobunaga M

    更新日期:1992-03-01 00:00:00

  • Inhibition of superoxide anion release from human polymorphonuclear leukocytes by N-acetyl-galactosamine and N-acetyl-glucosamine.

    abstract::This study shows that N-acetyl-galactosamine and N-acetyl-glucosamine can diminish the production of superoxide anion from cytochalasin-B treated PMNs stimulated with FMLP. Inhibition ranged from 80.9% to 1.8%. N-acetyl-galactosamine was superior to N-acetyl-glucosamine, but both showed their action in a dose-related ...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/BF02207968

    authors: Kamel M,Alnahdi M

    更新日期:1992-06-01 00:00:00

  • Syphilitic myositis: a case-based review.

    abstract::To present the clinical and pathological findings in patients presenting with myositis caused by syphilis. The literature is reviewed, and pathophysiologic factors discussed. A 49-year-old Caucasian heterosexual male with a known history of stable human immunodeficiency virus (HIV) and hepatitis C (HCV) co-infection, ...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章,评审

    doi:10.1007/s10067-010-1668-z

    authors: Yacyshyn E,Chiowchanwisawakit P,Emery DJ,Jhamandas J,Resch L,Taylor G

    更新日期:2011-05-01 00:00:00

  • Prevalence of nail involvement in nodal osteoarthritis.

    abstract::The presence of nail changes was investigated in 102 patients with nodal osteoarthritis of the hand. Nail lesions were seen in 14/102 patients (13.7%), a prevalence higher than that observed in 96 controls (4/96, 4.2%; chi square 4.37, p = 0.036). An increased risk of nail changes was associated with osteoarthritis of...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/BF02249013

    authors: Cimmino MA,Seriolo B,Accardo S

    更新日期:1994-06-01 00:00:00

  • Vascular damage and lack of angiogenesis in systemic sclerosis skin.

    abstract::The aim of this study was to analyse microvascular damage and compensatory angiogenesis in skin from patients with systemic sclerosis (SSc) compared with systemic lupus erythematosus (SLE), Raynaud's phenomenon (RP) and healthy controls. Immunohistochemistry was used for skin biopsies (9 SSc, 10 SLE, 9 RP and 12 healt...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-003-0698-1

    authors: Konttinen YT,Mackiewicz Z,Ruuttila P,Ceponis A,Sukura A,Povilenaite D,Hukkanen M,Virtanen I

    更新日期:2003-09-01 00:00:00

  • The impact of depression, microvasculopathy, and fibrosis on development of erectile dysfunction in men with systemic sclerosis.

    abstract::This article is a small case series that aims to discuss the impact of depression, vascular, and fibrotic changes on development of erectile dysfunction (ED) in patients with systemic sclerosis (SSc). In this paper, we present five male patients with SSc, aged 30-48 years. All patients are nonsmokers, and their past m...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-007-0567-4

    authors: Ostojic P,Damjanov N

    更新日期:2007-10-01 00:00:00

  • Granzyme A-immunoreactive cells in synovial fluid in reactive and rheumatoid arthritis.

    abstract::Perforin and granzyme A co-localize in the cytotoxic granules of killer cells like cytotoxic T lymphocytes (CTL). Perforin is the cytolytic pore-forming protein, whereas the function of the homodimeric serine protease granzyme A and other members of the granzyme family is still unclear. Granzyme A-immunoreactive cells...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/BF02283113

    authors: Nordström DC,Konttinen YT,Sorsa T,Nykanen P,Pettersson T,Santavirta S,Tschopp J

    更新日期:1992-12-01 00:00:00

  • Chronic eosinophilic pneumonia associated with an initiation of rheumatoid arthritis.

    abstract::Although peripheral blood eosinophilia is observed in patients with active inflammatory rheumatoid arthritis (RA), RA is not a recognised cause of pulmonary eosinophilia. We describe a 55-year-old woman affected by chronic eosinophilic pneumonia (CEP) concomitantly with an initiation of RA. Both diseases responded rap...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-003-0703-8

    authors: Kwak JJ,Chang JE,Lee J,Cho YJ,Sung SH

    更新日期:2003-09-01 00:00:00

  • The role of the unfolded protein response in axial spondyloarthritis.

    abstract::Susceptibility to ankylosing spondylitis is highly genetic, with a heritability greater than 90 %. Presence of the HLA-B27 MHC class I allele remains the greatest genetic risk factor identified to date. Beyond its nominal role in antigen presentation, HLA-B27 displays interesting and possibly unique biochemical charac...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章,评审

    doi:10.1007/s10067-015-3117-5

    authors: Smith JA

    更新日期:2016-06-01 00:00:00

  • Correction to: Neutrophil/lymphocyte ratio and platelet/lymphocyte ratio in Behçet's disease: which and when to use?

    abstract::The authors wish to acknowledge that whilst this study and that published in Hammad et al [1] explore different areas - blood cell ratios and serum endocan, respectively - the reader may identify some similarities between the papers owing to the same patient cohort being analysed in both studies. ...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章,已发布勘误

    doi:10.1007/s10067-019-04583-5

    authors: Hammad M,Shehata OZ,Abdel-Latif SM,El-Din AMM

    更新日期:2019-07-01 00:00:00

  • Macrophage-colony stimulating factor (M-CSF) is increased in the synovial-like membrane of the periprosthetic tissues in the aseptic loosening of total hip replacement (THR).

    abstract::The aim of the study was to assess the eventual presence, cellular localization and extent of expression of the osteoclast activating cytokine M-CSF (CSF-1) in the periprosthetic tissues around loose total hip replacement (THR). Synovial-like membrane was obtained from the implant-to-bone interface and pseudocapsule f...

    journal_title:Clinical rheumatology

    pub_type: 临床试验,杂志文章

    doi:10.1007/BF02238958

    authors: Xu JW,Konttinen YT,Waris V,Pätiälä H,Sorsa T,Santavirta S

    更新日期:1997-05-01 00:00:00

  • Long-term efficacy and safety of interferon α-2a therapy in severe refractory ophthalmic Behcet's disease.

    abstract::Ophthalmic involvement is the most debilitating complication of Behcet's disease (BD). The aim of the current study is to report on the efficacy and safety of a long-term use of interferon alpha-2a (IFNα-2a) in the treatment of refractory ophthalmic BD in the Azari population of Iran. We retrospectively analyzed the c...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-016-3318-6

    authors: Kavandi H,Khabbazi A,Kolahi S,Hajialilo M,Shayan FK,Oliaei M

    更新日期:2016-11-01 00:00:00

  • HLA antigens in palindromic rheumatism. An Italian study.

    abstract::Forty-four patients with typical palindromic rheumatism were typed for HLA-A,B,C and DR antigens. The mean duration of follow-up of our patients was 4.0 yrs. (range 1-12 yrs.). During this time 27% of patients showed a seropositivity; 22% of patients developed polyarthritis, 1 patient revealed a Sjögren syndrome and 3...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:

    authors: Barbieri P,Ciompi ML,Menicucci A,Pasero G

    更新日期:1988-12-01 00:00:00

  • Bleomycin-induced scleroderma: report of a case with a chronic course rather than the typical acute/subacute self-limiting form.

    abstract::We report a case of bleomycin-induced scleroderma in a 35-year-old woman treated with chemotherapy for Hodgkin's disease. Approximately 6 months after the first chemotherapy cycle, the patient developed skin sclerosis in both arms. The lesion showed no signs of spontaneous clinical amelioration and treatment with ster...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s100670050131

    authors: Passiu G,Cauli A,Atzeni F,Aledda M,Dessole G,Sanna G,Nurchis P,Vacca A,Garau P,Laudadio M,Mathieu A

    更新日期:1999-01-01 00:00:00

  • Raynaud's phenomenon: clinical spectrum of 118 patients.

    abstract:OBJECTIVES:To establish the prevalence of the associated secondary diseases in patients with Raynaud's phenomenon (RP) attending a rheumatology specialty centre and to determine the frequency with which a secondary illness develops in those having RP, in absence of a well-defined secondary cause. METHODS:118 consecuti...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-003-0726-1

    authors: De Angelis R,Del Medico P,Blasetti P,Cervini C

    更新日期:2003-10-01 00:00:00

  • Bone mass in Zaireans: radiogrammetric determination.

    abstract::Bone mass was measured in 230 subjects with ages ranging from 15 to 54 years (110 males and 120 females), using the radiogrammetric method on the second metacarpal bone. Results of the study show that bone mass increases with age up to 34 years and then decreases, both in males and females, although males have signifi...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/BF02208090

    authors: Kabeya JM,Mbuyi Muamba JM,Tozin R,Dequeker J

    更新日期:1995-01-01 00:00:00

  • Cutaneous mucinosis associated with multiple frozen joints and bony heterotopic deposits around the hips.

    abstract::A 43-year-old man suffered from migrating then additive arthralgias associated with macroscopic and microscopic cutaneous lesions compatible at first sight with reticular erythematous mucinosis. After nine months, the cutaneous picture evolved into a papular mucinosis. The patient developed "multiple frozen joints" wi...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/BF02032364

    authors: Dufour JP,Lachapelle JM,Nagant de Deuxchaisnes C,Devogelaer JP,Noel H,Maldague B

    更新日期:1986-06-01 00:00:00

  • Cross-cultural adaptation and validation of the osteoporosis assessment questionnaire short version (OPAQ-SV) for Chinese osteoporotic fracture females.

    abstract::The Osteoporosis Assessment Questionnaire Short Version (OPAQ-SV) was cross-culturally adapted to measure health-related quality of life in Chinese osteoporotic fracture females and then validated in China for its psychometric properties. Cross-cultural adaptation, including translation of the original OPAQ-SV into Ma...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-015-3010-2

    authors: Zhang YP,Wei HH,Wang W,Xia RY,Zhou XL,Porr C,Lammi M

    更新日期:2016-04-01 00:00:00

  • Acute phase proteins and clinical synovitis activity in patients with rheumatoid arthritis.

    abstract::A number of laboratory variables, including Hb., ESR and several phase proteins, fluctuated in concord with the clinical signs of synovitis activity in patients with rheumatoid arthritis during a controlled study of 3 disease-modifying anti-rheumatic drugs (DMARD). The correlation between laboratory variables and clin...

    journal_title:Clinical rheumatology

    pub_type: 临床试验,杂志文章

    doi:10.1007/BF02201028

    authors: Bentzon MW,Gad I,Halberg P,Halskov O,Lorenzen I

    更新日期:1987-06-01 00:00:00

  • Interactions and perceptions of patients with rheumatoid arthritis participating in an online support group.

    abstract:OBJECTIVE:Peer support is important for psychosocial well-being in patients with rheumatoid arthritis (RA). Our objective was to assess the interactions, engagement, and perceptions of participants in an online support group for patients with RA. METHODS:Participants were 18 years or older, diagnosed with RA within 10...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-020-04967-y

    authors: des Bordes JKA,Foreman J,Westrich-Robertson T,Lopez-Olivo MA,Peterson SK,Hofstetter C,Lyddiatt A,Willcockson I,Leong A,Suarez-Almazor ME

    更新日期:2020-06-01 00:00:00

  • COX-2-Specific inhibitors--the emergence of a new class of analgesic and anti-inflammatory drugs.

    abstract::The prostaglandin series of bioactive compounds is formed by the interaction of two distinct but related enzymes, cyclo-oxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). COX-1 is a constitutive form which is present mainly in the gastric mucosa, kidney and platelets. COX-2 is mainly an inducible form, although also to...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章,评审

    doi:10.1007/s100670070024

    authors: Everts B,Währborg P,Hedner T

    更新日期:2000-01-01 00:00:00

  • Efficacy of transforaminal versus interspinous corticosteroid injectionin discal radiculalgia - a prospective, randomised, double-blind study.

    abstract::A prospective, randomised, double-blind study was carried out to compare the respective efficacies of transforaminal and interspinous epidural corticosteroid injections in discal radiculalgia. Thirty-one patients (18 females, 13 males) with discal radicular pain of less than 3 months' duration were consecutively rando...

    journal_title:Clinical rheumatology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1007/s10067-003-0736-z

    authors: Thomas E,Cyteval C,Abiad L,Picot MC,Taourel P,Blotman F

    更新日期:2003-10-01 00:00:00

  • Frequency and spectrum of outpatient musculoskeletal diagnoses at a pediatric hospital in Kenya.

    abstract::Chronic pediatric musculoskeletal (MSK) conditions are a major cause of morbidity. The burden of pediatric rheumatic diseases in East Africa is largely unknown. The purposes of this study were to estimate frequencies and assess the spectrum of MSK-related diagnoses using ICD-10 diagnostic codes among outpatients at a ...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-018-4376-8

    authors: Scuccimarri R,Broten L,Migowa A,Ngwiri T,Wachira J,Bernatsky S,Hitchon C,Colmegna I

    更新日期:2019-04-01 00:00:00

  • Evolution of chronic kidney disease in patients with systemic lupus erythematosus over a long-period follow-up: a single-center inception cohort study.

    abstract::The objective is to investigate the accrual rate and risk factors of chronic kidney disease (CKD) in an inception cohort of patients with systemic lupus erythematosus (SLE) followed at a single tertiary center. A prospectively collected database of 256 consecutive patients with SLE followed over a 25-year period was s...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-014-2527-0

    authors: Pokroy-Shapira E,Gelernter I,Molad Y

    更新日期:2014-05-01 00:00:00

  • Quality of life in patients with Takayasu's arteritis is impaired and comparable with rheumatoid arthritis and ankylosing spondylitis patients.

    abstract::The aims of the study were to assess the health-related quality of life (QOL) in patients with Takayasu's arteritis (TA) by two different generic QOL instruments and to compare the results with those patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS), and healthy controls (HC). A cross-sectional stud...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-007-0813-9

    authors: Akar S,Can G,Binicier O,Aksu K,Akinci B,Solmaz D,Birlik M,Keser G,Akkoc N,Onen F

    更新日期:2008-07-01 00:00:00

  • Ultrasound elastography-based assessment of the elasticity of the supraspinatus muscle in impingement syndrome: does elastography has any diagnostic value?

    abstract::Ultrasound elastography (UE) is a new ultrasound-based imaging technique that provides information about elasticity and stiffness of tissues. This cross-sectional study aimed to identify the diagnostic importance of UE in supraspinatus impingement syndrome. Forty-one subjects, aged 38-70 years, were included in the st...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-017-3928-7

    authors: Demirel A,Baykara M,Koca TT,Berk E

    更新日期:2018-06-01 00:00:00

  • Adult onset Still's disease diagnosed concomitantly with occult papillary thyroid cancer: paraneoplastic manifestation or coincidence?

    abstract::Adult onset Still's disease (AOSD) is an inflammatory disease of unknown etiology, characterized by spiking fever, evanescent salmon pink maculopapular rash, arthritis, and leukocytosis with neutrophilia. Malignant lymphoma is one of the most important differential diagnoses of AOSD. AOSD has been reported as one of p...

    journal_title:Clinical rheumatology

    pub_type: 杂志文章

    doi:10.1007/s10067-009-1305-x

    authors: Ahn JK,Oh JM,Lee J,Kim SW,Cha HS,Koh EM

    更新日期:2010-02-01 00:00:00