Evaluation of the Commercial Kit SIRE Nitratase for detecting resistant Mycobacterium tuberculosis in Brazil.

Abstract:

INTRODUCTION::This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates. METHODS::The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method with Lowenstein-Jensen medium or the BACTEC MGIT 960 system. RESULTS::The detection accuracy for streptomycin, isoniazid, rifampicin, and ethambutol was 95%, 97.5%, 100%, and 80%, respectively. CONCLUSIONS::The exceptional accuracy demonstrated by Kit SIRE Nitratase for isoniazid and rifampicin makes the kit an attractive option for screening M. tuberculosis strain resistance.

journal_name

Rev Soc Bras Med Trop

authors

Miranda SS,Almeida IN,Lopes ML,Figueiredo JDR,Figueredo LJA,Kritski AL,Carvalho WDS,Mansur MFFO

doi

10.1590/0037-8682-0447-2016

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

550-553

issue

4

eissn

0037-8682

issn

1678-9849

pii

S0037-86822017000400550

journal_volume

50

pub_type

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