Abstract：
:Human promyelocytic cells (HL-60) were labeled with 35S-sulfate and either 3H-glucosamine or 3H-serine as precursors. Accumulation of 35S-labeled macromolecules was approximately linear for up to 96 h, with a mean cell:medium ratio of 5.5:1, although activity/10(5) viable cells reached a plateau level after 24 h. Virtually none of the cell-associated proteoglycan was removed by trypsinization, consistent with a predominantly intracellular localization. Proteoglycan heterogeneity was investigated by DEAE-Sephacel chromatography, isopyknic CsCl gradient centrifugation, and gel filtration chromatography. HL-60 cells appeared to synthesize a single proteoglycan species, Kav = 0.46 on Sepharose CL-4B and Kav = 0.32 on Sepharose CL-6B, recovered primarily from the high-density fractions of a dissociative CsCl gradient (rho greater than 1.40 g/l). Degradation products of lower charge density, lower buoyant density, and lower hydrodynamic size were also present, mainly in the cell pellets. The major proteoglycan was found to contain chondroitin sulfate chains of average Mr = 14.5 kD, yielding virtually 100% 4-sulfated disaccharides on digestion with chondroitinase ABC. The proteoglycan was resistant to trypsin, chymotrypsin, plasmin, and papain, and the core protein Mr was approximately 20 kD by molecular sieve chromatography. Induction of HL-60 cells with 0.15 dimethyl sulfoxide (DMSO) resulted in differentiation to a more mature granulocytic phenotype and was associated with a reduction in 35S-sulfate incorporation to 45% of control values or 32%, expressed as activity/10(5) cells. Proteoglycans synthesized by DMSO-treated cells were identical to those from untreated cells in terms of hydrodynamic size, glycosaminoglycan Mr, and sulfation.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Bentley SA,Kirby SLdoi
10.1002/jlb.45.1.46subject
Has Abstractpub_date
1989-01-01 00:00:00pages
46-54issue
1eissn
0741-5400issn
1938-3673journal_volume
45pub_type
杂志文章abstract：:PGG-glucan (Betafectin) is a soluble, highly purified yeast (1,3)-beta-glucan with broad anti-infective and immunomodulatory activities. These studies evaluated the ability of PGG-glucan to directly elicit O2- and tumor necrosis factor alpha (TNF-alpha) production by rat leukocytes in vitro. Particulate beta-glucan st...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.64.3.337
更新日期：1998-09-01 00:00:00
abstract：:To understand the modulation and the behavior of glycocalyx elements during adhesion, we explored one of its components, the CD43 molecule, on human monocytic THP-1 cells exposed to cytokine stimulation and its redistribution during heterotypic adhesion to opsonized erythrocytes. First we demonstrated by immunofluores...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.61.5.609
更新日期：1997-05-01 00:00:00
abstract：:C1qR(P) is a type I cell surface glycoprotein that has been shown to enhance ingestion of suboptimally opsonized targets by phagocytes in vitro. In this study, we developed and characterized polyclonal antibodies to study the tissue distribution of this receptor targeted to either the N- or C-terminal portion of the m...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:The receptor for urokinase plasminogen activator (uPAR; CD87) is a 50- to 65-kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed by leukocytes and tumor cells where it facilitates uPA-dependent, plasmin-mediated pericellular proteolysis during cellular invasion. Because uPAR is inducibly shed into c...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.64.2.203
更新日期：1998-08-01 00:00:00
abstract：:Dendritic cells (DCs) are specialized APCs present in all tissues, including tumors. They play a major role in orchestrating immune responses and were shown to occur in various functional states in tumors. In this respect, immunogenic tumor-associated DCs (TADCs) are required to initiate and sustain T cell-dependent a...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.4MR1116-466R
更新日期：2017-08-01 00:00:00
abstract：:It has been shown that lead (Pb) potentiates lipopolysaccharide (LPS) lethality in animals by increasing the secretion and uptake or reactivity of tumor necrosis factor-alpha (TNF-alpha). Herein we report that PbCl2 increased TNF-alpha secretion from LPS-treated human peripheral blood mononuclear cells (PBMC) in a con...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.59.6.932
更新日期：1996-06-01 00:00:00
abstract：:In RAW 264.7 cells, PKC-ε regulates FcγR-mediated phagocytosis. BMDM behave similarly; PKC-ε concentrates at phagosomes and internalization are reduced in PKC-ε⁻/⁻ cells. Two questions were asked: what is the role of PKC-ε? and what domains are necessary for PKC-ε concentration? Function was studied using BMDM and fru...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1212634
更新日期：2013-07-01 00:00:00
abstract：:Alpha-defensins are antimicrobial peptides that contribute to innate-immune functions of neutrophils and intestinal Paneth cells. Transcription of alpha-defensin genes occurs early in neutrophilic myelopoeisis. To examine the mechanisms that regulate alpha-defensin gene expression, we analyzed transcription of rat neu...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0803384
更新日期：2004-02-01 00:00:00
abstract：:THP-1-derived cell lines were stably transfected with constructs encoding glycophosphatidylinositol (GPI)-anchored or transmembrane forms of human CD14. CD14 expression was associated with enhanced phagocytosis of serum (heat-inactivated)-opsonized Escherichia coli (opEc). Both the GPI-anchored and transmembrane forms...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.62.6.786
更新日期：1997-12-01 00:00:00
abstract：:In this study we present data on a novel cell surface antigen recognized by monoclonal antibody (mAb) VPM30, originally thought to recognize only bovine and ovine sIg+ B cells from peripheral blood. Here we show that the antigen, molecular mass 28 kDa, is not only found in B cell follicles in frozen sections, but when...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.63.5.567
更新日期：1998-05-01 00:00:00
abstract：:Retroviral infections are frequently associated with immunosuppression. Retroviral transmembrane envelope proteins (TM proteins) play an important role in this phenomenon. CKS-17, a synthetic heptadecapeptide, represents the immunosuppressive site of these retroviral TM proteins. Here we support on the further delinea...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.51.3.282
更新日期：1992-03-01 00:00:00
abstract：:Single-cell RNA sequencing (scRNA-seq) is a powerful new technology allowing the analysis of transcriptomes from individual cell and is ideally suited to dissect immune cell heterogeneity. ScRNA-seq has already been applied to identify novel immune cell subsets, elaborate cellular differentiation trajectories, and elu...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1002/JLB.5MR1019-179R
更新日期：2020-04-01 00:00:00
abstract：:In previous studies, macrophages have been shown to influence in vitro colony formation by erythroid progenitors (CFU-E). To determine whether the origin of the macrophages influences their effects on in vitro erythropoiesis, plasma clot cultures of CFU-E were grown over adherent monolayers of macrophages from various...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.36.5.581
更新日期：1984-11-01 00:00:00
abstract：:The mannose receptor, present on the plasma membrane of macrophages, promotes the internalization of glycoproteins and glycoconjugates via both endocytic and phagocytic pathways. The expression of this receptor is tightly modulated during monocyte/Mphi differentiation and cellular activation. We isolated clonal popula...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.64.1.85
更新日期：1998-07-01 00:00:00
abstract：:Radioprotective 105 kDa (CD180) is an unusual TLR that lacks an intracellular Toll-IL-1R signaling domain and exhibits unconventional homodimerization behavior. Differential expression and functions of radioprotective 105 kDa have been associated with immune-mediated pathologies, including infection, chronic inflammat...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.2VMR1215-582R
更新日期：2017-01-01 00:00:00
abstract：:The CD2 receptor on T-lymphocytes plays a major part in mediating adhesive interactions via the LFA-3 ligand and in transducing signals for lymphocyte activation. In this study the expression, function, and internalization of the CD2 receptor was investigated in resting and activated murine T-cells. Surface iodination...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.49.4.329
更新日期：1991-04-01 00:00:00
abstract：:Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultur...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.66.3.489
更新日期：1999-09-01 00:00:00
abstract：:Soluble mediators and inducible cell-surface and matrix-bound molecules coordinate the cascade of events giving rise to leukocyte emigration. Knowledge of the specific mechanisms underlying the attraction of cells into a local site, however, remains sketchy. In particular, it is unclear how chemoattractants cause rapi...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.58.4.438
更新日期：1995-10-01 00:00:00
abstract：:Cytokines are pivotal in the generation and resolution of the inflammatory response. The midkine/pleiotrophin (MK/PTN) family of cytokines, composed of just two members, was discovered as heparin-binding neurite outgrowth-promoting factors. Since their discovery, expression of this cytokine family has been reported in...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：10.1189/jlb.3MR1116-475R
更新日期：2017-08-01 00:00:00
abstract：:Lymphocyte function-associated antigen (LFA-1) is a member of the beta2 family of integrins that is selectively expressed on leukocytes. Herein, we show that Ca(2)(+) mobilizing agents A23187, thapsigargin, and ionomycin induce an increase in adhesion to the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1) and ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Neutrophils are quickly recruited to tissues in response to proinflammatory cues; however, little is known about tissue neutrophil phenotypes in health. We employ a multicolor flow cytometric approach to assess surface markers of activation on neutrophils from the bone marrow, blood, peritoneum, spleen, liver, fat, co...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/JLB.4AB1020-248RRR
更新日期：2020-11-04 00:00:00
abstract：:Healthy donors infused with high doses of glucocorticoids [GCs; methyl-prednisolone (MP); 500 mg/day for 3 days] suffer a selective depletion of the CD14(+)CD16(+) monocytes such that these cells are reduced by 95% on day 5. In vitro studies revealed that at 11 h of culture in the presence of 10(-)(5) M MP, no depleti...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.1202612
更新日期：2003-07-01 00:00:00
abstract：:Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptide...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0403138
更新日期：2003-12-01 00:00:00
abstract：:Nitric oxide (NO), the molecule of the year 1992, is gaining recognition as an important biological mediator. Its multitude of physiologic and pathophysiologic functions result from both a wide distribution of synthesis and diverse mechanisms of action. Besides its functions as a potent vasodilator and neurotransmitte...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章,评审
doi：
更新日期：1993-08-01 00:00:00
abstract：:TLR are primary triggers of the innate immune system by recognizing various microorganisms through conserved pathogen-associated molecular patterns. TLR2 is the receptor for a functional recognition of bacterial lipopeptides (LP) and is up-regulated during various disorders such as chronic obstructive pulmonary diseas...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0807586
更新日期：2008-03-01 00:00:00
abstract：:Neutrophil accumulation and plasma leakage induced in rabbit skin by neutrophil-activating peptide-1 (NAP-1, a 72 amino acid peptide produced by monocytes and a variety of tissue cells), E. coli endotoxin, and interleukin-1 (IL-1) were compared. Neutrophil accumulation at sites injected with NAP-1 was intense, rapid, ...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.48.2.129
更新日期：1990-08-01 00:00:00
abstract：:The recently cloned ligand binding component of the type I human interferon-alpha/beta receptor (IFN-alpha/beta R) and its soluble analogue (p40) were characterized. p40 is a potent inhibitor of type I IFNs and antibodies directed against p40 completely block the activity of type I IFNs in human cells. These antibodie...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.57.5.712
更新日期：1995-05-01 00:00:00
abstract：:Alum, aluminum-hydroxide-containing compounds, long used as adjuvants in human vaccinations, functions by ill-defined, immunostimulatory mechanisms. Antigen-free alum has been shown to act via a previously unidentified, splenic Gr1(+), IL-4-expressing myeloid cell population to stimulate early B cell priming. We demon...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1189/jlb.0607392
更新日期：2008-04-01 00:00:00
abstract：:The effects of the carbocyclic nucleoside MDL 201,112 and the purine nucleoside adenosine on the interferon-gamma (IFN-gamma)-induced priming of macrophages (m phi s) for the respiratory burst and major histocompatibility class II (MHC class II) Ia+ antigen expression were compared. Priming of purified, peritoneal m p...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.56.2.133
更新日期：1994-08-01 00:00:00
abstract：:Rat thymocytes respond to exposure to phytomitogens by oxidant generation as detected by chemiluminescence in presence of luminol. Maximal chemiluminescent response to a wide variety of plant lectins was obtained only after the cells had been incubated for 18 hours at 37 degrees C but not at 4 degrees C. This temperat...
journal_title：Journal of leukocyte biology
pub_type： 杂志文章
doi：10.1002/jlb.35.4.427
更新日期：1984-04-01 00:00:00
