Abstract:
:The mannose receptor, present on the plasma membrane of macrophages, promotes the internalization of glycoproteins and glycoconjugates via both endocytic and phagocytic pathways. The expression of this receptor is tightly modulated during monocyte/Mphi differentiation and cellular activation. We isolated clonal populations from murine J774 macrophage tumor cells, which differ in their surface expression of functional mannose receptors. To examine the potential mechanisms regulating receptor function in these cell lines, the interaction of receptor with ligand as well as receptor synthesis and degradation was analyzed. J774 clones with both high and low levels of mannose receptor activity were found to synthesize significant amounts of receptor protein, suggesting that the protein may be regulated at the level of synthesis and degradation. In J774 clones expressing very low receptor activity and protein, the half-life of mannose receptor molecules was substantially decreased. The evolution of multiple mechanisms modulating mannose receptor function may be critical in fine-tuning the role of this receptor in antigen processing and in scavenger and host defense functions.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Fiani ML,Beitz J,Turvy D,Blum JS,Stahl PDdoi
10.1002/jlb.64.1.85subject
Has Abstractpub_date
1998-07-01 00:00:00pages
85-91issue
1eissn
0741-5400issn
1938-3673journal_volume
64pub_type
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