Abstract:
PURPOSE:The need for clinically applicable skin substitutes continues to be a matter of fact. Hypothetically, a laboratory grown autologous skin analog with near normal architecture might be a suitable approach to yield both satisfactory functional and cosmetic long-term results. In this study, we explored the use of human endothelial cells derived from freshly isolated adipose stromal vascular fraction (SVF) in a three-dimensional (3D) co-culture model of vascularized bio-engineered skin substitute. METHODS:The SVF was isolated from human white adipose tissue samples and keratinocytes from human skin biopsies. The SVF, in particular endothelial cells, were characterized using flow cytometry and immuofluorescence analysis. Endothelial and mesenchymal progenitors from the SVF formed blood capillaries after seeding into a 3D collagen type I hydrogel in vitro. Subsequently, human keratinocytes were seeded on the top of those hydrogels to develop a vascularized dermo-epidermal skin substitute. RESULTS:Flow cytometric analysis of surface markers of the freshly isolated SVF showed the expression of endothelial markers (CD31, CD34, CD146), mesenchymal/stromal cell-associated markers (CD44, CD73, CD90, CD105), stem cell markers (CD49f, CD117, CD133), and additionally hematopoietic markers (CD14, CD15, CD45). Further analysis of white adipose-derived endothelial cells (watECs) revealed the co-expression of CD31, CD34, CD90, CD105, and partially CD146 on these cells. WatECs were separated from adipose-stromal cells (watASCs) using FACS sorting. WatASCs and watECs cultured separately in a 3D hydrogel for 3 weeks did not form any vascular structures. Only if co-cultured, both cell types aligned to develop a ramified vascular network in vitro with continuous endothelial lumen formation. Transplantation of those 3D-hydrogels onto immuno-incompetent rats resulted in a rapid connection of human capillaries with the host vessels and formation of functional, blood-perfused mosaic human-rat vessels within only 3-4 days. CONCLUSIONS:Adipose tissue represents an attractive cell source due to the ease of isolation and abundance of endothelial as well as mesenchymal cell lineages. Adipose-derived SVF cells exhibit the ability to form microvascular structures in vitro and support the accelerated blood perfusion in skin substitutes in vivo when transplanted.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Klar AS,Güven S,Zimoch J,Zapiórkowska NA,Biedermann T,Böttcher-Haberzeth S,Meuli-Simmen C,Martin I,Scherberich A,Reichmann E,Meuli Mdoi
10.1007/s00383-015-3808-7subject
Has Abstractpub_date
2016-01-01 00:00:00pages
17-27issue
1eissn
0179-0358issn
1437-9813pii
10.1007/s00383-015-3808-7journal_volume
32pub_type
杂志文章abstract::Colonic interposition is a treatment option in childhood when esophageal replacement (ER) is necessary. We reviewed 18 children who underwent ER by colon between 1984 and 1999. There were 5 with esophageal atresia and 13 with corrosive esophagitis; 15 had long-term follow-up (mean 38 months). Three procedures were per...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000422
更新日期:2000-01-01 00:00:00
abstract::A previous study documented the effects of maternal corticosteroid treatment on structural growth and functional development in fetal lungs associated with pathogenic conditions such as oligohydramnios using immunohistochemical and morphometric analyses. The purpose of the present study was to examine the effect of ma...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-1979-6
更新日期:2007-10-01 00:00:00
abstract::An infant with a large-bowel obstruction due to Salmonella B enterocolitis is presented. The clinical and radiologic findings were suggestive of Hirschsprung's disease with total colonic aganglionosis. Due to further deterioration, an ileostomy was performed. Pathologic examination disclosed ganglia in the colon. At l...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003839900332
更新日期:2000-01-01 00:00:00
abstract::Macrophages (MPs) produce increased levels of proinflammatory cytokines in Crohn's disease; these cytokines are thought to play a central role in the occurrence of the disease. Biologics are currently available for anti-cytokine therapy, but treating intestinal inflammation through direct suppression of proinflammator...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1767-8
更新日期:2006-11-01 00:00:00
abstract:PURPOSE:Haemolytic uraemic syndrome (HUS) is the commonest childhood cause of acute renal failure. Peritoneal dialysis peritonitis (PDP) is a well-recognised complication, with some children requiring surgical intervention (SI). The aim of this study is to determine whether the presence of enteric organisms in cases of...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3833-6
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Children with intussusception require rapid and accurate diagnosis to enable timely intervention for satisfactory outcome. Ultrasonography is the recommended standard diagnostic modality; however, abdominal radiography (AR) is still used as an initial investigation. The aim of this study was to investigate t...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3817-6
更新日期:2016-01-01 00:00:00
abstract::This review gives an overview of the disease spectrum of congenital diaphragmatic hernia (CDH). Etiological factors, prenatal predictors of survival, new treatment strategies and long-term morbidity are described. Early recognition of problems and improvement of treatment strategies in CDH patients may increase surviv...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-009-2425-8
更新日期:2009-09-01 00:00:00
abstract::Obstruction of the ureterovesical junction is an uncommon but well-recognized complication of ureteral reimplantation that traditionally has been treated by surgical correction [1, 5-9]. We report our experience with antegrade balloon dilation (ABD) of these strictures in two children. Obstruction was confirmed by diu...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF00626067
更新日期:1996-10-01 00:00:00
abstract::For the assessment of germ-cell maturation of the seminiferous tubules, DNA flowcytometry is a rapid and sensitive method. Phosporus 31P magnetic resonance spectroscopy (MRS) is a non-invasive alternative that demonstrates the metabolic status of the testis, reflecting the type and relative proportion of germ cells in...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830100602
更新日期:2001-05-01 00:00:00
abstract::A new instrument for suction rectal biopsy in infants suspected of having Hirschsprung's disease is described that can be completely dismantled, physically cleaned, lubricated, and heat-sterilised. These attributes are new and are absolutely critical today. The instrument has been used in 60 patients (ca. 180 biopsies...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01194812
更新日期:1997-01-01 00:00:00
abstract::The incidence of gastroschisis appears to be rising in developed nations, with epidemiological studies indicating association with young maternal age and smoking. Is there an association between maternal smoking and the development of complicated gastroschisis? A retrospective population-based review of all cases of g...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-1926-6
更新日期:2007-09-01 00:00:00
abstract::The aim of this study was to compare the safety and benefits of Soave's and Duhamel's pull-through procedures for the management of Hirschsprung's disease (HD). The patients consisted of 33 boys (85%) and six girls (15%), a ratio of 5.5:1. Their ages ranged from 1 day to 8 years. Rectal biopsy was performed to confirm...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1237-0
更新日期:2004-08-01 00:00:00
abstract:UNLABELLED:Necrotizing enterocolitis (NEC) is a serious condition with a high morbidity and mortality commonly affecting premature babies. Data for the impact of the long-term disease burden in developing countries are limited although poor long-term outcome of surgically managed patients has been shown in terms of inc...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2583-8
更新日期:2010-04-01 00:00:00
abstract:PURPOSE:To date, no detailed study of the changes in the image-defined risk factors (IDRFs) after neoadjuvant chemotherapy has been performed. The aim of this study was to investigate the effect of chemotherapy on IDRFs for stage L2 neuroblastomas. METHODS:Fifteen stage L2 patients treated by neoadjuvant chemotherapy ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3858-5
更新日期:2016-03-01 00:00:00
abstract::The advances in science have resulted in the emergence of nanotechnology, which deals with the design and use of tools and devices of size 1-100 nm. The application of nanotechnologies to medicine is thus termed nanomedicine. Significant research has been focused on this new and exciting field and this review article ...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-012-3162-y
更新日期:2012-10-01 00:00:00
abstract::Intestinal ischemia-reperfusion (IIR) is a complex phenomenon causing local and remote tissue destruction, and even multiple-organ failure. To examine the hypothesis that IIR affects renal function, 21-day-old male Sprague-Dawley rats underwent 45 min superior mesenteric artery occlusion and control rats were subjecte...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0834-z
更新日期:2002-09-01 00:00:00
abstract:PURPOSE:Although Rex shunt is an effective treatment for extrahepatic portal venous obstruction (EHPVO), 4-20% children develop rebleeding postoperatively. This study was used to evaluate the surgical treatment of rebleeding after Rex shunt in our center. METHOD:From June 2008 to Jan 2017, 12 of 122 children with EHPV...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4246-0
更新日期:2018-05-01 00:00:00
abstract::Ovarian involvement of acute lymphoblastic leukemia (ALL) without relapse in the bone marrow or other extramedullary site is rare. We report a case of ovarian relapse in a 13-year-old girl who was diagnosed with ALL at the age of 11. A complete remission was induced with the Children Cancer Group 1882 protocol for hig...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-008-2253-2
更新日期:2008-11-01 00:00:00
abstract::The Thomsen-Friedenreich cryptantigen (TCA) is located on the surface of all red cells, but is concealed by a layer of neuraminic acid. When bacteria that produce neuraminidase disrupt this coating, the TCA can be exposed and activated. If blood products containing antibody to the TCA are subsequently administered, ha...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050488
更新日期:1998-12-01 00:00:00
abstract:PURPOSE:The purpose of this study was to review the clinical characteristics and prognosis of children with adrenocortical tumors (ACT). METHODS:We retrospectively reviewed the medical records of 28 patients with ACT at our hospital between March 2010 and March 2017. RESULTS:The main clinical presentations were sexua...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4409-z
更新日期:2019-03-01 00:00:00
abstract::To assess the relation between continence and the manometric and endosonographic state of the anorectal segment after surgery for anorectal anomalies (ARA), 33 adolescents operated upon for ARA and 14 controls were examined. Seventeen patients had low and 16 intermediate or high ARA. Fecal continence was recorded, and...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01258715
更新日期:1997-09-01 00:00:00
abstract:AIM:We assessed continence after scope-assisted anorectovaginoplasty (SARVP) for female anorectal malformation (FARM). METHODS:Five FARM cases were assessed; cases 1 and 2: cloacal malformation; case 3: urogenital sinus, and rectovestibular fistula (RF); case 4: RF, absent vagina, and sacral anomaly; case 5: covered c...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3141-3
更新日期:2012-09-01 00:00:00
abstract::Thyroglossal duct cysts are one of the most commonly encountered benign neck lumps found in the paediatric population. Despite their relative frequency, reports of familial inheritance are rare. A total of 21 patients with hereditary thyroglossal duct cysts from seven families worldwide have been reported. The most co...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1420-y
更新日期:2005-07-01 00:00:00
abstract:PURPOSE:To study the gender assignment and hormonal treatment advocated for disorders of sexual differentiation (DSD). METHODS:A study was done on patients who were reviewed in the Pediatric Intersex Clinic to evaluate the pattern of gender assignment and hormonal treatment advocated. RESULTS AND CONCLUSION:The patie...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2232-7
更新日期:2008-10-01 00:00:00
abstract:PURPOSE:This study evaluates the capacity of ultrasonography as a diagnostic method to confirm the proper positioning of central venous catheter (CVC) when compared to the current gold standard, chest radiography (CR). METHODS:A prospective study was performed including children from 0 to 14 incomplete years, who unde...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04642-y
更新日期:2020-05-01 00:00:00
abstract::We report the case of a female adolescent who had an ectopic ovary in the inguinal canal without an associated hernia, a unicornuate uterus, and ipsilateral renal agenesis. The incidental discovery of the ectopic ovary and other Mullerian anomalies, as well as the surgical correction that followed, highlights importan...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-014-3582-y
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:The RAS association domain family protein 1 (RASSF1A) is known to be frequently inactivated by promoter hypermethylation in cancers. This study investigated the association of RASSF1A methylation with clinical outcomes in hepatoblastoma patients and whether it is correlated with the histological phenotype of he...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3371-z
更新日期:2013-11-01 00:00:00
abstract::A rare case of congenital duodenal atresia (DA) associated with a choledochal cyst (CC) is reported. At 38 weeks of gestation, a 1,610-g girl was born by cesarean section with a prenatal diagnosis of DA. After the disorder was confirmed by X-ray, she underwent a duodenoduodenostomy for a complete separation of the duo...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830100703
更新日期:2002-05-01 00:00:00
abstract::Juvenile papillomatosis (JP) is a rare benign, proliferative breast tumor in children. We observed a 9-year-old girl with a large soft-tissue mass at the lower outer quadrant of the left breast. Ultrasonography showed an ill-defined, inhomogeneous mass with numerous small, hypoechoic areas. The tumor was completely ex...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000462
更新日期:2001-03-01 00:00:00
abstract::Laparoscopy enables surgeons to approach the surgical conditions from a new perspective. Laparoscopic surgery has revolutionized the treatment of choledochal cysts (CDC). Yet, this new technique requires objective evaluations. We have examined the controversies about the CDC dissection, distal common bile duct ligatio...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-013-3266-z
更新日期:2013-04-01 00:00:00