Evaluation of developing rat testis by phosporus 31P magnetic resonance spectroscopy and DNA flowcytometry.


:For the assessment of germ-cell maturation of the seminiferous tubules, DNA flowcytometry is a rapid and sensitive method. Phosporus 31P magnetic resonance spectroscopy (MRS) is a non-invasive alternative that demonstrates the metabolic status of the testis, reflecting the type and relative proportion of germ cells in the testis. This study was designed to evaluate the utility of 31P MRS in reflecting the haploid-cell population of the testis as measured by DNA flowcytometry. A single testicle was evaluated in male Wistar pre-pubertal rats at 30, 40, 50, and 60 days of age. In order to minimize the contamination of signals from the contralateral testis, scrotum, and tail, the technique was modified and the testis was evaluated ex-vivo with an intact blood supply. At 30 days of age the percentage of haploid cells was 43.6 +/- 1.8, and this increased to 72.7 +/- 1.4 at 60 days of age. During this period, the testicular phosphomonoester/adenosine triphosphate (PM/ATP) ratio changed from 1.70 +/- 0.21 to 0.32 +/- 0.08. There was a significant (P < 0.001) linear correlation between the proportion of haploid cells evaluated by DNA flowcytometry and the PM/ATP ratio evaluated by 31P MRS. 31P MRS is thus a reliable, noninvasive technique for accurately assessing the status of the seminiferous epithelium.


Pediatr Surg Int


Srinivas M,Degaonkar M,Gupta DK,Raghunathan P,Das SN,Mitra DK




Has Abstract


2001-05-01 00:00:00












  • Correction to: Development of a new reproductive tissue cryopreservation clinical service for children: the Oxford programme.

    abstract::In the published version, the Acknowledgements section was missing a funding note of co-author Dr C Verrill. The corrected version should read as follows. ...

    journal_title:Pediatric surgery international

    pub_type: 已发布勘误


    authors: Lakhoo K,Davies J,Chakraborty S,Berg S,Tennyson R,Fowler D,Manek S,Verrill C,Lane S

    更新日期:2020-04-01 00:00:00

  • Could adding magnesium as adjuvant to ropivacaine in caudal anaesthesia improve postoperative pain control?

    abstract::Recently, most studies reported magnesium as a N-methyl-D-aspartate receptor antagonist and its analgesic and perioperative anaesthetic effects have been discussed with central desensitization pathway. We investigated the effects of caudal ropivacaine plus magnesium and compared with ropivacaine alone on postoperative...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章,随机对照试验


    authors: Birbicer H,Doruk N,Cinel I,Atici S,Avlan D,Bilgin E,Oral U

    更新日期:2007-02-01 00:00:00

  • The vulnerable stomach in babies born with pure oesophageal atresia.

    abstract::Nine babies with pure oesophageal atresia were treated in our institution in the years 1979-1996. All received a feeding gastrostomy as their initial operation. After initiation of gastrostomy feeds seven (78%) developed gastric complications, including two posterior gastric perforations (one fatal). We propose that t...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Kimble RM,Harding JE,Kolbe A

    更新日期:1999-01-01 00:00:00

  • Can we improve outcome of congenital diaphragmatic hernia?

    abstract::This review gives an overview of the disease spectrum of congenital diaphragmatic hernia (CDH). Etiological factors, prenatal predictors of survival, new treatment strategies and long-term morbidity are described. Early recognition of problems and improvement of treatment strategies in CDH patients may increase surviv...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章,评审


    authors: van den Hout L,Sluiter I,Gischler S,De Klein A,Rottier R,Ijsselstijn H,Reiss I,Tibboel D

    更新日期:2009-09-01 00:00:00

  • Neonatal surgery in low- vs. high-volume institutions: a KID inpatient database outcomes and cost study after repair of congenital diaphragmatic hernia, esophageal atresia, and gastroschisis.

    abstract:BACKGROUND/PURPOSE:The volume-outcome relationship and optimal surgical volumes for repair of congenital anomalies in neonates is unknown. METHODS:A retrospective study of infants who underwent diaphragmatic hernia (CDH), gastroschisis (GS), and esophageal atresia/tracheoesophageal fistula (EA/TEF) repair at US hospit...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Sømme S,Shahi N,McLeod L,Torok M,McManus B,Ziegler MM

    更新日期:2019-11-01 00:00:00

  • The influence of gastroschisis on plasmatic coagulation, humoral immunity, and C-reactive protein.

    abstract::Around the 30th week of gestation, patients with gastroschisis (GS) develop chronic inflammatory reactions on the serosal surface of the eventrated loops of intestine that lead to severe hyperfibrinogenemia and hypercoagulability, particularly if they are born prematurely. This result was found on comparison of coagul...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Limbach HG,Quinten A,Jesberger HJ,Karadiakkos N,Lipfert S

    更新日期:1996-03-01 00:00:00

  • Distributions of substance P- and VIP-immunoreactive nerve fibres in the colonic circular muscle in children.

    abstract::In children with severe chronic constipation, abnormal distribution of substance P (SP) and vasoactive intestinal peptide (VIP), which represent excitatory and inhibitory nerves, respectively, has been reported. The normal distribution of these neuropeptides, however, is not well known. The aim of this study was to de...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Uemura S,Hurley MR,Hutson JM,Chow CW

    更新日期:1998-11-01 00:00:00

  • Role of the surgeon in non-accidental trauma.

    abstract::Non-accidental trauma (NAT) represents a significant cause of morbidity and mortality in the pediatric population. The management of these patients often involves many care providers including the surgeon. Victims of NAT often present with multiple injuries and as such should be treated as trauma patients with complet...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章,评审


    authors: Naik-Mathuria B,Akinkuotu A,Wesson D

    更新日期:2015-07-01 00:00:00

  • Hypoxia-regulated gene expression in fetal wound regeneration and adult wound repair.

    abstract::Fetal skin wounds heal scarlessly while adult wounds scar. Fetal wound healing occurs in a physiologically hypoxic environment whereas in adult wound healing, cells have to acutely adapt to hypoxia caused by locally impaired blood supply. We examined the expression of hypoxia-inducible factor 1 (HIF-1), a potent trans...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Scheid A,Wenger RH,Christina H,Camenisch I,Ferenc A,Stauffer UG,Gassmann M,Meuli M

    更新日期:2000-01-01 00:00:00

  • What is the optimal surgical strategy for complex perianal fistulous disease in pediatric Crohn's disease? A systematic review.

    abstract:PURPOSE:Perianal fistulous disease is present in 10-15% of children with Crohn's disease (CD) and is frequently complex and refractory to treatment, with one-third of patients having recurrent lesions. We conducted a systematic review of the literature to examine the best surgical strategy or strategies for pediatric c...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章,评审


    authors: Kantor N,Wayne C,Nasr A

    更新日期:2017-05-01 00:00:00

  • Esophageal atresia and tracheo-esophageal fistula associated with coarctation of the aorta, CHARGE association, and DiGeorge syndrome: a case report and literature review.

    abstract::CHARGE association and DiGeorge syndrome (DGS) rarely occur together and only eight cases have been reported in the English literature. Two were associated with esophageal atresia (EA) and severe congenital heart anomalies. We report a third case of EA with tracheoesophageal fistula (EA-TEF) associated with coarctatio...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章,评审


    authors: Lee KD,Okazaki T,Kato Y,Lane GJ,Yamataka A

    更新日期:2008-10-01 00:00:00

  • Universal expression of cell adhesion molecule NCAM in neuroblastoma in contrast to L1: implications for different roles in tumor biology of neuroblastoma?

    abstract:PURPOSE:Neuroblastoma is a biological, genetic and morphological heterogeneous tumor with a variable clinical course. NCAM is a cell adhesion molecule belonging to the immunoglobulin superfamily with structural similarities to cell adhesion molecule L1. The aim of this study was to determine the expression of NCAM in n...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Wachowiak R,Rawnaq T,Metzger R,Quaas A,Fiegel H,Kähler N,Rolle U,Izbicki JR,Kaifi J,Till H

    更新日期:2008-12-01 00:00:00

  • In-vitro uptake of radioactive lipiodol I-131 and I-125 by hepatoblastoma: implications for targeted radiotherapy.

    abstract::Lipiodol, a stable iodine addition product of fatty ethyl esters derived from poppyseed oil, has been used as a vehicle for targeted cytotoxic or radiotherapeutic treatment in adults with unresectable hepatocellular carcinoma and in a few children with advanced hepatoblastoma (HB). Prolonged retention of lipiodol by t...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Towu E,Boxer G,Begent R,Zweit J,Spitz L,Hobbs K,Winslet M

    更新日期:2001-11-01 00:00:00

  • Laparoscopic surgery for acute neonatal appendicitis.

    abstract::We present the first two cases of acute neonatal appendicitis operated on through the laparoscopic approach. Acute neonatal appendicitis is uncommon and rarely considered by clinicians when assessing the neonatal acute abdomen. Our two cases demonstrate the potential value of diagnostic laparoscopy in the acute neonat...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Malakounides G,John M,Rex D,Mulhall J,Nandi B,Mukhtar Z

    更新日期:2011-11-01 00:00:00

  • Management of gastrointestinal perforation from blunt and penetrating abdominal trauma in children: analysis of 96 patients.

    abstract:AIM:The objective of the present study was to evaluate the diagnostic methods, concomitant organ injuries, factors affecting mortality and morbidity, treatment methods, and outcomes of patients treated for traumatic gastrointestinal (GI) perforation. MATERIALS AND METHODS:We conducted a retrospective review of the med...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Arslan S,Okur MH,Arslan MS,Aydogdu B,Zeytun H,Basuguy E,Icer M,Goya C

    更新日期:2016-11-01 00:00:00

  • Cholelithiasis in early infancy.

    abstract::This report describes three children, age range 7 weeks to 5 months, who presented with obstructive jaundice secondary to gallstones. Previous Escherichia coli septicaemia and frusemide therapy were predisposing risk factors in two of the patients. All three were successfully treated with cholecystectomy and explorati...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Wilcox DT,Casson D,Bowen J,Thomas A,Bruce J

    更新日期:1997-02-01 00:00:00

  • Increased intracellular levels of calcitonin gene-related peptide-like immunoreactivity in pulmonary endocrine cells in an experimental model of congenital diaphragmatic hernia.

    abstract::A congenital diaphragmatic hernia (CDH) model was induced in pregnant rats following administration of 100 mg nitrofen. The fetuses were stored and fixed in Bouin's solution for 24 h after caesarean section at term. After fixation, the lungs were dissected out. Immunostaining of the CDH lungs and controls with rabbit ...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Yamataka T,Puri P

    更新日期:1996-08-01 00:00:00

  • Classification and diagnostic criteria of variants of Hirschsprung's disease.

    abstract::"Variants of Hirschsprung's disease" are conditions that clinically resemble Hirschsprung's disease (HD), despite the presence of ganglion cells in rectal suction biopsies. The diagnosis and management of these patients can be challenging. Specific histological, immunohistochemical and electron microscopic investigati...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章,评审


    authors: Friedmacher F,Puri P

    更新日期:2013-09-01 00:00:00

  • Postoperative abdominal evisceration in children: possible risk factors.

    abstract::Postoperative abdominal evisceration may lead to high morbidity and mortality. The purpose of the present study was to determine possible risk factors, morbidity and mortality associated with abdominal evisceration in children. A total of 3,591 children underwent abdominal exploration in our clinic between 1983 and 20...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Ciğdem MK,Onen A,Otçu S,Duran H

    更新日期:2006-08-01 00:00:00

  • Isolated bowel segment created by omentoenteropexy: histologic findings.

    abstract::An isolated bowel segment (IBS) is a viable loop of bowel that is completely free of its mesenteric attachments. In this study, omentoenteropexy (OEP) was performed in 5-cm-long IBS of rats in order to provide neovascularization. Thirty-two rats were divided into four groups. After OEP, mesenteric division of the IBSs...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Günel E,Tavll L,CaGlayan F,GündoGan AH

    更新日期:1997-07-01 00:00:00

  • Rhabdoid tumour of the kidney: a diagnostic challenge and a fatal outcome.

    abstract::Rhabdoid tumour of the kidney is not common. Presentation of such a lethal tumour along with blunt abdominal trauma is even rarer. We report such a case of Rhabdoid tumour of kidney which presented as renal trauma, and discuss the diagnostic difficulties and rapidly fatal outcome. ...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Desai SR,Upadhyay V

    更新日期:2000-01-01 00:00:00

  • Retrocaval ureter in children: a report of two cases.

    abstract::Retrocaval ureter is a relatively rare anomaly that usually manifests in the third or fourth decades. Symptoms are due to ureteric obstruction, either extrinsic by the abnormal inferior vena cava (IVC), or intrinsic ureteric hypoplasia. Surgery is needed for symptomatic cases and involves transection and relocation of...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Soundappan SV,Barker AP

    更新日期:2004-02-01 00:00:00

  • Plasticity of the enteric nervous system in patients with intestinal neuronal dysplasia associated with Hirschsprung's disease: a report of three patients.

    abstract::Intestinal neuronal dysplasia is a controversial form of dysganglionosis that has been described both as an isolated disorder and in association with Hirschsprung's disease. It has been blamed for the bad outcome of bowel function in patients operated on for Hirschsprung's disease. According to various authors, intest...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Meyrat BJ,Laurini RN

    更新日期:2003-12-01 00:00:00

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    abstract::To evaluate the efficacy of ultrasound (US) guided percutaneous sclerotherapy in the pediatric population, 14 hydatid liver cysts (HLC) in eight male patients whose ages ranged between 6 and 16 years (mean 9.9 years) were treated. The maximum diameter was 110 mm. Albendazole was administered orally to all patients for...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Kabaalioğlu A,Karaali K,Apaydin A,Melikoğlu M,Sindel T,Lüleci E

    更新日期:2000-01-01 00:00:00

  • One-staged or two-staged surgery for perforated choledochal cyst with bile peritonitis in children? A single center experience with 27 cases.

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    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Ngoc Son T,Thanh Liem N,Manh Hoan V

    更新日期:2014-03-01 00:00:00

  • Robotic fundoplication in children.

    abstract::Since January 2006, robotic assistance has been used for performing minimal invasive laparoscopic fundoplications in children. These patients were compared with those operated on with either the open surgical technique or the laparoscopic procedure. The first six children operated on with a fundoplication using the op...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Anderberg M,Kockum CC,Arnbjörnsson E

    更新日期:2007-02-01 00:00:00

  • Surgery results in significant improvement in growth in children with Crohn's disease refractory to medical therapy.

    abstract::Inflammatory bowel disease (IBD) in children can cause significant impairment in linear growth, and delay in pubertal onset. The aim of this study was to assess the impact of surgery on linear growth in children with Crohn's disease (CD) who were resistant to medical therapy, and had documented evidence of growth impa...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Singh Ranger G,Lamparelli MJ,Aldridge A,Chong SK,Mitton SG,Albanese A,Kumar D

    更新日期:2006-04-01 00:00:00

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    abstract:BACKGROUND:Vitamin D deficiency is associated with intestinal barrier dysfunction, which contributes to pathogenesis of acute intestinal injury in children. We aim to investigate the effects of vitamin D on intestinal injury in intestinal epithelial cells and organoids. METHODS:Lipopolysaccharide (LPS) was used to ind...

    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Lee C,Lau E,Chusilp S,Filler R,Li B,Zhu H,Yamoto M,Pierro A

    更新日期:2019-12-01 00:00:00

  • The management of anorectal malformation with congenital vestibular fistula: a single-stage modified anterior sagittal anorectoplasty.

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    journal_title:Pediatric surgery international

    pub_type: 杂志文章


    authors: Wang C,Li L,Liu S,Chen Z,Diao M,Li X,Qiao G,Cheng W

    更新日期:2015-09-01 00:00:00

  • Stroma-predominant Wilms tumor with teratoid features: report of a rare case and review of the literature.

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    journal_title:Pediatric surgery international

    pub_type: 杂志文章,评审


    authors: Gupta R,Sharma A,Arora R,Dinda AK

    更新日期:2009-03-01 00:00:00