Abstract:
:For the assessment of germ-cell maturation of the seminiferous tubules, DNA flowcytometry is a rapid and sensitive method. Phosporus 31P magnetic resonance spectroscopy (MRS) is a non-invasive alternative that demonstrates the metabolic status of the testis, reflecting the type and relative proportion of germ cells in the testis. This study was designed to evaluate the utility of 31P MRS in reflecting the haploid-cell population of the testis as measured by DNA flowcytometry. A single testicle was evaluated in male Wistar pre-pubertal rats at 30, 40, 50, and 60 days of age. In order to minimize the contamination of signals from the contralateral testis, scrotum, and tail, the technique was modified and the testis was evaluated ex-vivo with an intact blood supply. At 30 days of age the percentage of haploid cells was 43.6 +/- 1.8, and this increased to 72.7 +/- 1.4 at 60 days of age. During this period, the testicular phosphomonoester/adenosine triphosphate (PM/ATP) ratio changed from 1.70 +/- 0.21 to 0.32 +/- 0.08. There was a significant (P < 0.001) linear correlation between the proportion of haploid cells evaluated by DNA flowcytometry and the PM/ATP ratio evaluated by 31P MRS. 31P MRS is thus a reliable, noninvasive technique for accurately assessing the status of the seminiferous epithelium.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Srinivas M,Degaonkar M,Gupta DK,Raghunathan P,Das SN,Mitra DKdoi
10.1007/s003830100602subject
Has Abstractpub_date
2001-05-01 00:00:00pages
316-20issue
4eissn
0179-0358issn
1437-9813journal_volume
17pub_type
杂志文章abstract::In the published version, the Acknowledgements section was missing a funding note of co-author Dr C Verrill. The corrected version should read as follows. ...
journal_title:Pediatric surgery international
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abstract::Nine babies with pure oesophageal atresia were treated in our institution in the years 1979-1996. All received a feeding gastrostomy as their initial operation. After initiation of gastrostomy feeds seven (78%) developed gastric complications, including two posterior gastric perforations (one fatal). We propose that t...
journal_title:Pediatric surgery international
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journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
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doi:10.1007/s00383-019-04525-x
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journal_title:Pediatric surgery international
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journal_title:Pediatric surgery international
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doi:10.1007/s003830050438
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journal_title:Pediatric surgery international
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journal_title:Pediatric surgery international
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journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
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journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
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abstract:PURPOSE:Neuroblastoma is a biological, genetic and morphological heterogeneous tumor with a variable clinical course. NCAM is a cell adhesion molecule belonging to the immunoglobulin superfamily with structural similarities to cell adhesion molecule L1. The aim of this study was to determine the expression of NCAM in n...
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pub_type: 杂志文章
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830100004
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journal_title:Pediatric surgery international
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doi:
更新日期:1997-02-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF00180080
更新日期:1996-08-01 00:00:00
abstract::"Variants of Hirschsprung's disease" are conditions that clinically resemble Hirschsprung's disease (HD), despite the presence of ganglion cells in rectal suction biopsies. The diagnosis and management of these patients can be challenging. Specific histological, immunohistochemical and electron microscopic investigati...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
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journal_title:Pediatric surgery international
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journal_title:Pediatric surgery international
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journal_title:Pediatric surgery international
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abstract::Retrocaval ureter is a relatively rare anomaly that usually manifests in the third or fourth decades. Symptoms are due to ureteric obstruction, either extrinsic by the abnormal inferior vena cava (IVC), or intrinsic ureteric hypoplasia. Surgery is needed for symptomatic cases and involves transection and relocation of...
journal_title:Pediatric surgery international
pub_type: 杂志文章
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journal_title:Pediatric surgery international
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doi:10.1007/s00383-003-1035-0
更新日期:2003-12-01 00:00:00
abstract::To evaluate the efficacy of ultrasound (US) guided percutaneous sclerotherapy in the pediatric population, 14 hydatid liver cysts (HLC) in eight male patients whose ages ranged between 6 and 16 years (mean 9.9 years) were treated. The maximum diameter was 110 mm. Albendazole was administered orally to all patients for...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000356
更新日期:2000-01-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3461-6
更新日期:2014-03-01 00:00:00
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journal_title:Pediatric surgery international
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
abstract::Inflammatory bowel disease (IBD) in children can cause significant impairment in linear growth, and delay in pubertal onset. The aim of this study was to assess the impact of surgery on linear growth in children with Crohn's disease (CD) who were resistant to medical therapy, and had documented evidence of growth impa...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1664-1
更新日期:2006-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Pediatric surgery international
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journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
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