Abstract:
:For the assessment of germ-cell maturation of the seminiferous tubules, DNA flowcytometry is a rapid and sensitive method. Phosporus 31P magnetic resonance spectroscopy (MRS) is a non-invasive alternative that demonstrates the metabolic status of the testis, reflecting the type and relative proportion of germ cells in the testis. This study was designed to evaluate the utility of 31P MRS in reflecting the haploid-cell population of the testis as measured by DNA flowcytometry. A single testicle was evaluated in male Wistar pre-pubertal rats at 30, 40, 50, and 60 days of age. In order to minimize the contamination of signals from the contralateral testis, scrotum, and tail, the technique was modified and the testis was evaluated ex-vivo with an intact blood supply. At 30 days of age the percentage of haploid cells was 43.6 +/- 1.8, and this increased to 72.7 +/- 1.4 at 60 days of age. During this period, the testicular phosphomonoester/adenosine triphosphate (PM/ATP) ratio changed from 1.70 +/- 0.21 to 0.32 +/- 0.08. There was a significant (P < 0.001) linear correlation between the proportion of haploid cells evaluated by DNA flowcytometry and the PM/ATP ratio evaluated by 31P MRS. 31P MRS is thus a reliable, noninvasive technique for accurately assessing the status of the seminiferous epithelium.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Srinivas M,Degaonkar M,Gupta DK,Raghunathan P,Das SN,Mitra DKdoi
10.1007/s003830100602subject
Has Abstractpub_date
2001-05-01 00:00:00pages
316-20issue
4eissn
0179-0358issn
1437-9813journal_volume
17pub_type
杂志文章abstract::We present a neonate with gastroschisis and evidence of bile aspiration in utero, who developed severe respiratory distress that did not respond to postnatal intensive respiratory care. Although rare, a newborn with gastroschisis may develop severe respiratory distress due to bile aspiration in utero. Given the poor o...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2223-8
更新日期:2008-10-01 00:00:00
abstract::Numerous case reports in the literature have suggested a causal link between beta-haemolytic streptococcal infection and delayed-onset right-sided diaphragmatic hernia/eventration in the perinatal age group. We report a child with antenatally documented right diaphragmatic eventration 4 weeks before birth who subseque...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:
更新日期:1997-03-21 00:00:00
abstract::Cryptorchidism is a common paediatric complaint and is traditionally managed surgically by orchidopexy. We aimed to prospectively review the success of the Bianchi high scrotal incision technique for performing orchidopexy in boys with palpable non-descended testis. A total of 206 orchidopexies were performed in 85 pa...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2149-1
更新日期:2008-06-01 00:00:00
abstract:INTRODUCTION:Management of oesophageal atresia (OA) and trachea-oesophageal fistula (TOF) in babies of low birth weight is challenging especially when associated with other anomalies. Birth weight of <1500 g has previously formed part of a classification system designed to predict outcome, alongside the cardiac status ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3851-4
更新日期:2016-06-01 00:00:00
abstract::A boy with a history of esophageal atresia repair received an esophagoesophagostomy at 14 months for a severe refractory anastomotic stricture, which had already required repeated balloon dilation with intralesional dexamethasone injection. Anastomotic leakage and stricture was again evident after the second surgery, ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-009-2367-1
更新日期:2009-06-01 00:00:00
abstract::Accidental ingestion of corrosive substances remains a major health hazard in children. Most infants and children who ingest caustic substances present with very few symptoms or signs. Approximately 40% of caustic substance ingestions result in esophageal injury, but the optimal management of caustic esophageal burns ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1331-3
更新日期:2005-02-01 00:00:00
abstract:PURPOSE:To describe the management of cavoatrial extension of nephroblastoma in a developing country. PATIENTS AND METHODS:Of 406 consecutive children with Wilms tumour, 40 (10%) presented with tumour extension into the inferior vena cava (IVC) (30), right atrium or right ventricle and form the inception cohort. Of th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2667-5
更新日期:2010-12-01 00:00:00
abstract::To assess the relation between continence and the manometric and endosonographic state of the anorectal segment after surgery for anorectal anomalies (ARA), 33 adolescents operated upon for ARA and 14 controls were examined. Seventeen patients had low and 16 intermediate or high ARA. Fecal continence was recorded, and...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01258715
更新日期:1997-09-01 00:00:00
abstract::An unrecognised right aortic arch (RAA) found at thoracotomy may complicate the repair of oesophageal atresia (OA) and tracheo-oesophageal fistula (TOF). This paper analyses the patient characteristics, peri-operative management, and outcome of 16 infants with a RAA, and proposes management guidelines. Between 1948 an...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050505
更新日期:1999-01-01 00:00:00
abstract::Children with chronic idiopathic constipation (CIC) often end up at the surgeon when medical treatments have failed. This opinion piece discusses a recently described pattern of CIC called 'Rapid transit constipation (RTC)' first identified in 2011 as part of surgical workup. RTC was identified using a nuclear medicin...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-019-04587-x
更新日期:2020-01-01 00:00:00
abstract::The aim of this study was to determine whether barium enema reduction (BER) is safe and effective in patients with a long duration of intussusception. Over the last 17 years, 104 patients were admitted to our hospital with a diagnosis of intussusception. All except 1 with peritonitis underwent BER primarily. Of the 10...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050526
更新日期:1999-01-01 00:00:00
abstract::In spite of advances in the treatment of childhood bladder and prostate rhabdomyosarcoma (RMS), the ability to detect minimal residual disease correlates imperfectly with the ultimate outcome. We report the long-term follow-up of a child with microscopic residual RMS after chemotherapy. The correct interpretation of t...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830200017
更新日期:2002-01-01 00:00:00
abstract:PURPOSE:We investigated the relationship between the affected lobe and symptom onset in prenatally diagnosed congenital pulmonary airway malformation (CPAM). METHODS:53 CPAM patients diagnosed prenatally were reviewed retrospectively by creating 2 groups according to symptom onset. Group Sneo: (symptomatic during the ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-019-04460-x
更新日期:2019-05-01 00:00:00
abstract:INTRODUCTION:The Morgagni hernia (MH) accounts for 3-4% of congenital diaphragmatic hernias. There is a paucity of data regarding this rare defect. The purpose of this study is to describe the characteristics of children with MH, surgical approaches for repair, and patient outcomes. METHODS:Pediatric patients (ages 0-...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4078-3
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Vitamin D deficiency is associated with intestinal barrier dysfunction, which contributes to pathogenesis of acute intestinal injury in children. We aim to investigate the effects of vitamin D on intestinal injury in intestinal epithelial cells and organoids. METHODS:Lipopolysaccharide (LPS) was used to ind...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-019-04586-y
更新日期:2019-12-01 00:00:00
abstract::In severe vaginal malformations, when the distance between the upper vaginal pouch and perineum is too long (6 cm or more), reconstruction of the vagina can be performed by colonic interposition or by long cutaneous flaps obtained by the tissue expansion technique. Two female adolescents were treated using expanded la...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract:PURPOSE:The aim of this study is to identify the risk factors for esophageal anastomotic stricture (EAS) and/or anastomotic leakage (EAL) after primary repair of esophageal atresia with tracheoesophageal fistula (EA/TEF) in infants. METHODS:A retrospective chart review of 52 patients with congenital EA/TEF between Jan...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3931-0
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:Pancreatic neoplasms are uncommon in children. This study sought to analyze the clinical and pathological features of surgically resected pancreatic tumors in children and discuss management strategies. METHODS:We conducted a retrospective review of patients ≤21 years with pancreatic neoplasms who underwent su...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3925-y
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:The Mac-2-binding protein glycosylation-modified isomer (M2BPGi) is a new marker for progression of hepatic fibrosis. We examined the relationship between serum M2BPGi levels and liver histological findings in patients with biliary atresia (BA) who were not transplant candidates. METHODS:Patients with BA who w...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4317-2
更新日期:2018-10-01 00:00:00
abstract::The incidence of postoperative cholangitis has changed very little despite progressive improvement in the treatment of biliary atresia. The role of the bilioenteric conduit in its pathogenesis is still uncertain. A retrospective study of 39 patients undergoing either a conventional Kasai operation (group 1, n = 20) or...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050008
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Retinoids are essential for fetal and lung development. Beta-carotene(BC) is the main dietary retinoid source and beta-carotene-15,15'-oxygenase-1 and 2 (Bcmo1,2) is the primary enzyme generating retinoid from BC in adult mammalian tissues. Placenta has a major role in the retinol homeostasis in fetal life: ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3621-8
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:Haemolytic uraemic syndrome (HUS) is the commonest childhood cause of acute renal failure. Peritoneal dialysis peritonitis (PDP) is a well-recognised complication, with some children requiring surgical intervention (SI). The aim of this study is to determine whether the presence of enteric organisms in cases of...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3833-6
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Subclinical varicocele is a relative rare condition. The aim of this study is to identify the role of bioflavonoids in the management of subclinical varicocele. MATERIALS AND METHODS:We analyzed the medical charts of patients treated for subclinical varicocele with bioflavonoids between 1999 and 2004; inclu...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2574-9
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVE:Surgical repair of pectus excavatum (PE) in childhood is a well-established procedure. Previously used operative techniques to correct PE were largely based on the Ravitch technique. Since about 10 years, the minimally invasive repair (MIRPE) by Nuss is well established. Conservative treatment with the vacuum...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2843-7
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:The optimal treatment for pediatric Graves' disease (GD) is controversial. Antithyroid drugs are often used initially, but they are associated with a high failure rate. Therefore alternative therapies have become important. In the present study, we analyze our institution's experience regarding the safety and e...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3095-5
更新日期:2012-06-01 00:00:00
abstract::Umbilical hernias occur frequently in children but complications are very rare and thus surgery is not routinely indicated. In this literature review, we report 19 cases of spontaneous evisceration of abdominal contents through umbilical hernias. Precipitating causes included umbilical ulceration or sepsis, crying, re...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-012-3076-8
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND/PURPOSE:To determine the frequency and characteristics of suspected cow's milk protein allergy (CMPA) in infants with gastroschisis and response to change in milk. METHODS:A retrospective cohort study of 111 consecutive infants with gastroschisis. RESULTS:64 episodes suggesting non-IgE-mediated CMPA occurr...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3664-5
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:Pediatric patients with intestinal failure need long-term parenteral nutrition (PN), but this nutritional support causes liver dysfunction, such as intestinal failure-associated liver disease (IFALD). Several studies have shown that the lipid emulsion produced by soybean oil (SO) is associated with the occurren...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4190-4
更新日期:2018-02-01 00:00:00
abstract::We present the case of a 14-year-old male with a history of small bowel transplantation for long segment Hirschsprung's disease who underwent Duhamel ileorectal pull-through procedure. In post-transplant, the patient had no restrictions and was not TPN-dependent. To improve his quality of life, he and his family were ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4216-y
更新日期:2018-01-01 00:00:00
abstract:AIM:We assessed continence after scope-assisted anorectovaginoplasty (SARVP) for female anorectal malformation (FARM). METHODS:Five FARM cases were assessed; cases 1 and 2: cloacal malformation; case 3: urogenital sinus, and rectovestibular fistula (RF); case 4: RF, absent vagina, and sacral anomaly; case 5: covered c...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3141-3
更新日期:2012-09-01 00:00:00