Abstract:
BACKGROUND:Vitamin D deficiency is associated with intestinal barrier dysfunction, which contributes to pathogenesis of acute intestinal injury in children. We aim to investigate the effects of vitamin D on intestinal injury in intestinal epithelial cells and organoids. METHODS:Lipopolysaccharide (LPS) was used to induce injury in intestinal epithelial cells (IEC-18) and organoids, and the effect of vitamin D was assessed. Cell viability was measured and inflammation cytokines TNFα and IL-8 were quantified. FITC-dextran 4 kDa (FD4) permeability was measured using Transwell while tight junction markers were assessed by immunofluorescence staining in IEC-18 and intestinal organoids. Data were compared using one-way ANOVA with Bonferroni post-test. RESULTS:IEC-18 viability was decreased by LPS treatment, but was prevented by vitamin D. The upregulation of inflammation was inhibited by vitamin D, which also decreased epithelium permeability. Vitamin D restored tight junction ZO-1 and claudin 2. In addition, vitamin D decreased TNFα expression and prevented the disruption of ZO-1 in injured organoids. CONCLUSIONS:Vitamin D rescued epithelial barrier function by improving permeability and restoring tight junctions, leading to decrease inflammation. This study confirms the protective effects of vitamin D, which could be used as a treatment strategy for infants at risk of developing intestinal injury.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Lee C,Lau E,Chusilp S,Filler R,Li B,Zhu H,Yamoto M,Pierro Adoi
10.1007/s00383-019-04586-ysubject
Has Abstractpub_date
2019-12-01 00:00:00pages
1395-1401issue
12eissn
0179-0358issn
1437-9813pii
10.1007/s00383-019-04586-yjournal_volume
35pub_type
杂志文章abstract::Congenital diaphragmatic hernia (CDH) is accompanied by pulmonary hypoplasia and pulmonary hypertension. Fetal lung growth is dependent on the secretion of lung liquid, which normally is absorbed at partus. The ion channel NKCC-1 is involved in this secretory process, but has recently also been reported to be implicat...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2209-6
更新日期:2008-09-01 00:00:00
abstract::Colonic interposition is a treatment option in childhood when esophageal replacement (ER) is necessary. We reviewed 18 children who underwent ER by colon between 1984 and 1999. There were 5 with esophageal atresia and 13 with corrosive esophagitis; 15 had long-term follow-up (mean 38 months). Three procedures were per...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000422
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:Pyriform sinus fistula (PSF), which originates from the third or fourth branchial pouch remnant, is relatively rare. In young children and adults, it is characterized by lateral neck infections and suppurative thyroiditis, while neonatal PSF presents as a large cervical cystic mass and causes respiratory distre...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-3008-z
更新日期:2012-01-01 00:00:00
abstract::Congenital/infantile fibrosarcoma (IFS) is a relatively rare form of fibrosarcoma diagnosed at birth or during early years of life and that differs from its adult counterpart because of a more favorable behavior. IFS is also known as cellular congenital mesoblastic nephroma, when it affects the kidney and is often but...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3670-7
更新日期:2015-04-01 00:00:00
abstract::Bronchogenic cysts are congenital malformations of the tracheobronchial tree. We describe a 20-month-old male who presented with persistent non-bilious emesis; manometry and imaging were consistent with esophageal achalasia. During a planned laparoscopic Heller myotomy, an intramural bronchogenic cyst was discovered i...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3994-y
更新日期:2017-01-01 00:00:00
abstract::Evolution of the present-day policy of conservative management of ruptured spleen has been hailed as one of the most notable advances in pediatric surgery. Until 1971, routine splenectomy used to be the sacrosanct treatment for splenic trauma. It was universally believed that non-operative management carried a high mo...
journal_title:Pediatric surgery international
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00383-003-0972-y
更新日期:2003-11-01 00:00:00
abstract::Manual reduction with the index finger (modified Hutchinson's maneuver) enabling simple and safe manual reduction of infantile intussusception is described. The procedure was used in four patients, two boys and two girls, ranging in age from 2 to 15 months, as greater than normal compression was necessary and/or seros...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050301
更新日期:1998-03-01 00:00:00
abstract::Polyps in gastrointestinal tract are mostly benign and result from hamartomas or lymphoid hyperplasia of submucosa. They usually occur as isolated lesions in children and are referred to as juvenile polyps. Multiple polyps with inherited origin are called polyposis and can be seen as a part of the syndrome. The polyps...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-020-04621-3
更新日期:2020-04-01 00:00:00
abstract::"Variants of Hirschsprung's disease" are conditions that clinically resemble Hirschsprung's disease (HD), despite the presence of ganglion cells in rectal suction biopsies. The diagnosis and management of these patients can be challenging. Specific histological, immunohistochemical and electron microscopic investigati...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-013-3351-3
更新日期:2013-09-01 00:00:00
abstract:PURPOSE:In 2006, The Japanese Society of Pediatric Endoscopic Surgeons devised a plan to develop a pediatric endoscopic surgical skill qualification (ESSQ) system. This system is controlled by The Japan Society for Endoscopic Surgery. The standard requirement for skills qualification is the ability of each applicant to...
journal_title:Pediatric surgery international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00383-011-2871-y
更新日期:2011-07-01 00:00:00
abstract::Anorectal injuries (ARI) are uncommon in children in civil practice. In developed countries the injuries are mainly due to sexual abuse and firearms. This report reviews the experience in tropical Africa. A retrospective study of children aged 12 years or less managed for ARI over 10 years was undertaken. There were s...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000347
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:The aim of this study was to analyze the outcomes of children added to the waiting list for deceased donor liver transplantation (DDLT) and the results of DDLT in a single Japanese center. METHODS:Forty-seven children were listed on the organ allocation system for DDLT. The priority points related to the medic...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3381-x
更新日期:2013-11-01 00:00:00
abstract::Reflux nephropathy (RN) is recognized as a major cause of end-stage renal failure in children and young adults. Inhibition of nitric oxide (NO) exacerbates and enhanced production ameliorates tubulointerstitial fibrosis (TIF) in experimental obstructive uropathy. NO is synthesised by NO synthase (NOS), three distinct ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0858-4
更新日期:2002-10-01 00:00:00
abstract:PURPOSE:Cervicothoracic and apical thoracic neuroblastoma pose unique surgical challenges. We report our experience with the trapdoor anterior thoracotomy (TAT) approach to overcome these difficulties. METHODS:Retrospective review of our centre's neuroblastoma database was conducted. Patients who underwent TAT at our ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04692-2
更新日期:2020-08-01 00:00:00
abstract::A 7-year-old female with a type I choledochal cyst underwent an exploratory laparotomy. A Roux-en-Y choledochojejunostomy was done following cyst excision. The patient was re-explored 8 days later for an anastomotic bile leak and high fever. A round-worm was found obstructing the jejunal lumen at the porta hepatis, re...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050394
更新日期:1998-09-01 00:00:00
abstract::Beckwith-Wiedemann Syndrome (BWS) is associated with somatic overgrowth and a high risk for embryonal tumors, including hepatoblastoma, a highly malignant liver tumor of childhood. Focal nodular hyperplasia (FNH), on the other hand, is a benign tumor of the liver that is uncommon in childhood. Herein we describe a cas...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1469-7
更新日期:2005-09-01 00:00:00
abstract:PURPOSE:We previously showed that meticulous probing and resection of the intrahepatic bile duct stenosis (IHBDS)-causing membrane or septum was effective in preventing hepatolithiasis after choledochal cyst excisions in open surgeries. Then, we introduced this maneuver into laparoscopic choledochal cyst excisions in 2...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4320-7
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:To compare the effect of home intravenous (IV) versus oral antibiotic therapy on complication rates and resource utilization following appendectomy for perforated appendicitis. METHODS:This was a randomized controlled trial of patients aged 4-17 with surgically treated perforated appendicitis from January 2011...
journal_title:Pediatric surgery international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00383-018-4343-0
更新日期:2018-12-01 00:00:00
abstract::Recently, laparoscopic orchidopexy has been accepted as the standard procedure of management for clinically impalpable testes. However, many techniques have been described for the delivery of the mobilized testes. In this report, a new approach is described. The use of a fourth "scroto-peritoneal" port, makes complete...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050372
更新日期:1998-07-01 00:00:00
abstract:PURPOSE:To compare open repair (OR) with thoracoscopic repair (TR) for congenital diaphragmatic hernia (CDH) in neonates. METHODS:Twenty-four neonatal CDH cases diagnosed prenatally or within 6 h of birth at our institute from 2002 to 2012 with mild pulmonary hypertension managed without inhaled nitric oxide, were stu...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3382-9
更新日期:2013-11-01 00:00:00
abstract::Rhizomelic chondrodysplasia punctata (RCP), a rare autosomal recessive disease characterized by a disorder of peroxisome metabolism, has been shown to affect multiple organ systems. A neonate presenting with a colonic perforation in the first few hours of life was subsequently diagnosed with RCP. A literature search r...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-005-1426-5
更新日期:2005-08-01 00:00:00
abstract:PURPOSE:Surgery of the inguinal canal is very common in infancy. Occasionally, injury of the bladder may occur with severe consequences. The aim of this paper was to warn young surgeons of this serious complication. METHODS:During the last 2 years, we observed four cases of bladder injury following surgery on inguinal...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2707-1
更新日期:2010-12-01 00:00:00
abstract:PURPOSE:We performed a systematic review and meta-analysis to compare the efficacy and safety between single-incision, transscrotal orchidopexy, and the traditional inguinal orchidopexy in children. METHODS:A systematic search of the electronic databases was conducted to identify studies compared the transscrotal orch...
journal_title:Pediatric surgery international
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00383-016-3956-4
更新日期:2016-10-01 00:00:00
abstract:AIM:To determine if follow-up by consultant pediatric surgeons (CPS) affects morbidity due to incarceration (INC) in children with indirect inguinal hernia (IH). METHODS:We educate parents so they can identify possible INC and advise them to attend our emergency department anytime for immediate review by on-call CPS. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章,多中心研究
doi:10.1007/s00383-011-3005-2
更新日期:2012-01-01 00:00:00
abstract::Esophageal hiatal hernias (EHH) are probably caused by congenital, traumatic, or iatrogenic factors, although the etiology remains unknown. EHHs may develop after abdominal wall closure for omphalocele or gastroschisis due to the increased intra-abdominal pressure, however, there have been few reports in the literatur...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050352
更新日期:1998-07-01 00:00:00
abstract:AIMS:The aim of this study was to assess the usefulness of PAS, β-catenin and Ki-67 in categorising hepatoblastomas (HBs) and their significance in predicting prognosis. In addition, we have also documented the various chemotherapy induced histologic changes in HBs. METHOD:In this retrospective observational study of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-017-4115-2
更新日期:2017-09-01 00:00:00
abstract::It is widely accepted that there are degenerative changes and decreased spermatogenesis in the contralateral descended testis (CDT) in unilateral undescended testis (UUDT). While some investigators have postulated that the mechanism may be related to primary (congenital) or secondary (autoimmune, vascular, and neural)...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000532
更新日期:2001-07-01 00:00:00
abstract::Bronchogenic cyst (BC), which develops from abnormal budding of the tracheal diverticulum or ventral foregut, is a congenital bronchopulmonary malformation. Localization of the BC varies depending on the level of the abnormal budding. Thoracic or abdominal-sited diaphragmatic lesions are the rarest presentations of th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2187-8
更新日期:2008-08-01 00:00:00
abstract::Pediatric vascular injuries are increasing in frequency and represent a challenging problem in pediatric surgical practice. Increased survival of low birth weight infants and advances in invasive diagnostic procedures have resulted in a dramatic increase in the number of these injuries. Formation of pseudoaneurysm of ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1238-z
更新日期:2004-08-01 00:00:00
abstract::Our case report details the clinical course and management of a neonate with acute life-threatening respiratory distress presenting soon after birth. She needed intubation and ventilation. A pericardial cyst was diagnosed after a CT scan and 2D echo investigation. As a temporizing procedure the fluid was tapped and th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-0970-0
更新日期:2003-11-01 00:00:00