Protective effects of vitamin D against injury in intestinal epithelium.

Abstract:

BACKGROUND:Vitamin D deficiency is associated with intestinal barrier dysfunction, which contributes to pathogenesis of acute intestinal injury in children. We aim to investigate the effects of vitamin D on intestinal injury in intestinal epithelial cells and organoids. METHODS:Lipopolysaccharide (LPS) was used to induce injury in intestinal epithelial cells (IEC-18) and organoids, and the effect of vitamin D was assessed. Cell viability was measured and inflammation cytokines TNFα and IL-8 were quantified. FITC-dextran 4 kDa (FD4) permeability was measured using Transwell while tight junction markers were assessed by immunofluorescence staining in IEC-18 and intestinal organoids. Data were compared using one-way ANOVA with Bonferroni post-test. RESULTS:IEC-18 viability was decreased by LPS treatment, but was prevented by vitamin D. The upregulation of inflammation was inhibited by vitamin D, which also decreased epithelium permeability. Vitamin D restored tight junction ZO-1 and claudin 2. In addition, vitamin D decreased TNFα expression and prevented the disruption of ZO-1 in injured organoids. CONCLUSIONS:Vitamin D rescued epithelial barrier function by improving permeability and restoring tight junctions, leading to decrease inflammation. This study confirms the protective effects of vitamin D, which could be used as a treatment strategy for infants at risk of developing intestinal injury.

journal_name

Pediatr Surg Int

authors

Lee C,Lau E,Chusilp S,Filler R,Li B,Zhu H,Yamoto M,Pierro A

doi

10.1007/s00383-019-04586-y

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

1395-1401

issue

12

eissn

0179-0358

issn

1437-9813

pii

10.1007/s00383-019-04586-y

journal_volume

35

pub_type

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