Abstract:
PURPOSE:The RAS association domain family protein 1 (RASSF1A) is known to be frequently inactivated by promoter hypermethylation in cancers. This study investigated the association of RASSF1A methylation with clinical outcomes in hepatoblastoma patients and whether it is correlated with the histological phenotype of hepatoblastoma tumors. METHODS:Seventy-four hepatoblastoma tumors were obtained from patients enrolled in the Japanese study group for pediatric liver tumor protocol-2. From nine formalin-fixed, paraffin-embedded specimens, we extracted DNA by dissection under a light microscope. We examined the methylation status of the RASSF1A promoter region by bisulfite pyrosequencing. RESULTS:Twenty-five (33.8 %) hepatoblastoma tumors were classified as having methylated RASSF1A. The RASSF1A methylation was significantly associated with metastatic tumors and a poor prognosis. Despite the complete resection, five pretreatment extent of disease II tumors showed recurrence or distant metastasis postoperatively. Among these cases, four tumors were found to show RASSF1A methylation. When compared to histologically different types of cell, RASSF1A methylation values in samples of the normal liver, fetal type, and embryonal type, were significantly elevated in ascending order. CONCLUSIONS:We confirmed that RASSF1A methylation is a significant prognostic indicator in hepatoblastomas, and it may become a promising molecular marker to stratify patients into appropriate risk groups.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Honda S,Miyagi H,Suzuki H,Minato M,Haruta M,Kaneko Y,Hatanaka KC,Hiyama E,Kamijo T,Okada T,Taketomi Adoi
10.1007/s00383-013-3371-zsubject
Has Abstractpub_date
2013-11-01 00:00:00pages
1147-52issue
11eissn
0179-0358issn
1437-9813journal_volume
29pub_type
杂志文章abstract:BACKGROUND:Preoperative factors have been correlated with pre-incision hypotension (PIH) in children undergoing surgery, suggesting that PIH can be predicted through preoperative screening. We studied blood pressure (BP) changes in the 12 min following the induction of anesthesia to study the incidence of post-inductio...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4359-5
更新日期:2018-12-01 00:00:00
abstract::Single-system ureteral ectopia (UE) encompasses a spectrum of malformations involving the bladder trigone, ureter, and kidney. The clinical presentation is variable, and both diagnostic and therapeutic problems are common. Reduced renal function in these patients may result from primary dysplasia, obstruction, vesicou...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000414
更新日期:2000-01-01 00:00:00
abstract::Glicentin, the main component of enteroglucagon, has trophic effects on intestinal mucosa. It may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. We have established an in vitro bioassay system for determining the effects of recombinant human glicentin on bacterial internalization by co...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-1895-9
更新日期:2007-06-01 00:00:00
abstract:AIM:The aim of this study was to examine the relationship between birth weight, pulmonary artery (PA) size, and outcome in congenital diaphragmatic hernia (CDH) to establish if PA size has prognostic value. METHODS:The subjects for this study were 39 consecutive left-sided CDH patients treated at our institution accor...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2899-z
更新日期:2011-08-01 00:00:00
abstract:UNLABELLED:Necrotizing enterocolitis (NEC) is a serious condition with a high morbidity and mortality commonly affecting premature babies. Data for the impact of the long-term disease burden in developing countries are limited although poor long-term outcome of surgically managed patients has been shown in terms of inc...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2583-8
更新日期:2010-04-01 00:00:00
abstract::Pediatric osteomyelitis commonly occurs in the long bones and has rarely been reported in small bones such as the vertebrae and ribs. Rib osteomyelitis occurs in approximately 1% or less of all cases of hematogenous osteomyelitis, and is usually caused by Staphylococcus aureus. We present a case of acute osteomyelitis...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1725-5
更新日期:2006-10-01 00:00:00
abstract::Hepatogastric fistula secondary to amebic liver abscess is extremely rare. Only three pediatric cases have been reported in the English literature. Percutaneous drainage of abscess along with parenteral metronidazole can prevent the need for extensive surgical intervention. Timely intervention is usually followed by c...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1743-3
更新日期:2006-10-01 00:00:00
abstract:PURPOSE:Adnexal torsion is a gynecologic emergency, requiring intervention for tissue preservation. At our institution, torsion is managed by pediatric surgeons or gynecologists. We evaluated differences between specialties to streamline evaluation for children with gynecological emergencies, develop a clinical pathway...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04782-1
更新日期:2021-01-01 00:00:00
abstract::It is widely accepted that there are degenerative changes and decreased spermatogenesis in the contralateral descended testis (CDT) in unilateral undescended testis (UUDT). While some investigators have postulated that the mechanism may be related to primary (congenital) or secondary (autoimmune, vascular, and neural)...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000532
更新日期:2001-07-01 00:00:00
abstract:PURPOSE:Perianal fistulous disease is present in 10-15% of children with Crohn's disease (CD) and is frequently complex and refractory to treatment, with one-third of patients having recurrent lesions. We conducted a systematic review of the literature to examine the best surgical strategy or strategies for pediatric c...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-017-4067-6
更新日期:2017-05-01 00:00:00
abstract::The use of a silo for temporary coverage of exposed viscera for newborns with gastroschisis has allowed gradual reduction of the externalized intestine into the abdominal cavity. However, there has not been an easy way to monitor blood perfusion to the intestine within the silo other than with visual examination. In a...
journal_title:Pediatric surgery international
pub_type: 临床试验,杂志文章
doi:10.1007/s00383-006-1726-4
更新日期:2006-09-01 00:00:00
abstract::Eight children with Morgagni hernia were operated between January 2000 and May 2005. Medical records of the patients were evaluated retrospectively. Ages of the patients were between 3.5 months and 9 years. The diaphragmatic defect was on the right in all patients except one. One patient had bilateral diapragmatic her...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1750-4
更新日期:2006-10-01 00:00:00
abstract:PURPOSE:Feeding and systemic hypoxia are major stresses inducing necrotizing enterocolitis (NEC). This study aims to investigate the role of systemic hypoxia in NEC and its effect before and after feeding. METHODS:Neonatal mice were studied in three groups. Control (N = 9): breast feeding; NEC A (N = 8), gavage feedin...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3997-8
更新日期:2016-12-01 00:00:00
abstract::The purpose of this study is to evaluate the efficacy of intraoperative radiation therapy (IORT) and the problem of securing the IORT field in advanced pediatric neuroblastoma. Between 1996 and 2005, 12 children received IORT for advanced pediatric neuroblastoma patients. Electron beam energies ranged from 10 to 12 Me...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-2065-9
更新日期:2007-12-01 00:00:00
abstract::Primary hydatid disease of the pancreas is rare. The authors report a 3-year-old girl who had a cystic mass in the head of the pancreas with no other viscera involved. At laparatomy the entire endocyst was totally extracted from the surrounding pancreatic parenchyma. The postoperative course was uneventful. She is cur...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050677
更新日期:1999-01-01 00:00:00
abstract::Recently, most studies reported magnesium as a N-methyl-D-aspartate receptor antagonist and its analgesic and perioperative anaesthetic effects have been discussed with central desensitization pathway. We investigated the effects of caudal ropivacaine plus magnesium and compared with ropivacaine alone on postoperative...
journal_title:Pediatric surgery international
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00383-006-1779-4
更新日期:2007-02-01 00:00:00
abstract::Inflammatory bowel disease (IBD) in children can cause significant impairment in linear growth, and delay in pubertal onset. The aim of this study was to assess the impact of surgery on linear growth in children with Crohn's disease (CD) who were resistant to medical therapy, and had documented evidence of growth impa...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-006-1664-1
更新日期:2006-04-01 00:00:00
abstract::In neonates requiring operation for necrotizing enterocolitis (NEC), the complications due to enterostomy (ES) and the need for another operation to restore continuity have prompted several surgeons to employ primary anastomosis (PA) after resection as the operative strategy of choice. Our objective was to compare pri...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-017-4126-z
更新日期:2017-11-01 00:00:00
abstract::Bronchogenic cyst (BC), which develops from abnormal budding of the tracheal diverticulum or ventral foregut, is a congenital bronchopulmonary malformation. Localization of the BC varies depending on the level of the abnormal budding. Thoracic or abdominal-sited diaphragmatic lesions are the rarest presentations of th...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2187-8
更新日期:2008-08-01 00:00:00
abstract:PURPOSE:The Nuss procedure is a minimally invasive procedure for the correction of pectus excavatum. It involves insertion of a substernal metal bar. A feared complication of any implantation procedure is infection, which often requires removal of the implanted device. This report describes the authors' experience with...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3145-z
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:To evaluate early postoperative results in a case controlled study following clinical use of stem cells in extrahepatic biliary atresia (EHBA). METHODS:From July 2005 to March 2008, 30 cases of suspected EHBA were divided in two groups in an intervention study. Group A received autologous mononuclear bone marr...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2712-4
更新日期:2011-01-01 00:00:00
abstract::The presacral cystic teratoma in an infant is reported. The cyst consisted solely of central nervous system tissue. This pathologic finding is extremely rare. A review of the literature reveals no previous report of a teratoma arising in this manner. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0831-2
更新日期:2002-12-01 00:00:00
abstract:PURPOSE:Bicycling is a well-liked sporting activity in which many children participate, and bicycle accidents are one of the most common causes of abdominal injuries in children. We evaluated the characteristics and outcomes of abdominal injuries due to bicycle accidents in children. PATIENTS AND METHODS:This study wa...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-013-3279-7
更新日期:2013-05-01 00:00:00
abstract::An experimental study was conducted to determine the end-results of two different defects on the anterior abdominal wall: an abdominal wall defect (AWD) versus an umbilical cord defect (UCD) using chick embryos. The AWD was created by leaving an intact skin bridge between the defect and the umbilical cord in group 1; ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01371904
更新日期:1997-01-01 00:00:00
abstract::Long chain fatty acids (LCFAs) appear to be powerful stimulants for small bowel adaptation in patients with short bowel syndrome (SBS). However, the dietary lipid content may alter intestinal lipid transport. The aim of this study was to investigate the effects of a high fat diet (HFD) on in vivo lipid absorption and ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-1016-3
更新日期:2003-07-01 00:00:00
abstract::Hirschsprung's disease is characterized by the absence of ganglion cells in the distal bowel and extends proximally for varying distances. In recent years, the purinergic P2Y receptors have begun to receive much attention as they have been recognised as major ATP receptors in many regions of the body, including the in...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-007-2044-1
更新日期:2008-01-01 00:00:00
abstract:PURPOSE:We previously showed that meticulous probing and resection of the intrahepatic bile duct stenosis (IHBDS)-causing membrane or septum was effective in preventing hepatolithiasis after choledochal cyst excisions in open surgeries. Then, we introduced this maneuver into laparoscopic choledochal cyst excisions in 2...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4320-7
更新日期:2018-10-01 00:00:00
abstract::The most common cystic lesion recognized antenatally is multicystic dysplastic kidney (MCDK). Recently, conservative management without nephrectomy has been advocated. The purpose of this study was to report our experience in the conservative management of unilateral MCDK. Between 1989 and 1997, 20 children with MCDK ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000449
更新日期:2001-01-01 00:00:00
abstract::Primary lymphedema of the penis (PLP) is a rare disease. We report a case in a 2-year-old, uncircumcised boy where the uninvolved inner preputial skin was unfurled to cover the penile shaft. The uninvolved inner preputial skin is often elongated, and provides a suture-free cover of sufficient length for the small peni...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000487
更新日期:2001-03-01 00:00:00
abstract::A simple, quick, painless, and versatile dressing that fulfills most of the criteria of an ideal hypospadias dressing is described. ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-004-1206-7
更新日期:2004-05-01 00:00:00