Abstract:
:In the healthy kidney, specialized cells called podocytes form a sophisticated blood filtration apparatus that allows excretion of wastes and excess fluid from the blood while preventing loss of proteins such as albumin. To operate effectively, this filter is under substantial hydrostatic mechanical pressure. Given their function, it is expected that the ability to apply mechanical force is crucial to the survival of podocytes. However, to date, podocyte mechanobiology remains poorly understood, largely because of a lack of experimental data on the forces involved. We perform quantitative, continuous, nondisruptive, and high-resolution measurements of the forces exerted by differentiated podocytes in real time using a recently introduced functional imaging modality for continuous force mapping. Using an accepted model for podocyte injury, we find that injured podocytes experience near-complete loss of cellular force transmission but that this loss of force is reversible under certain conditions. The observed changes in force correlate with F-actin rearrangement and reduced expression of podocyte-specific proteins. By introducing robust and high-throughput mechanical phenotyping and by demonstrating the significance of mechanical forces in podocyte injury, this research paves the way to a new level of understanding of the kidney. In addition, in an advance over established force mapping techniques, we integrate cellular force measurements with immunofluorescence and perform continuous long-term force measurements of a cell population. Hence, our approach has general applicability to a wide range of biomedical questions involving mechanical forces.
journal_name
Sci Advjournal_title
Science advancesauthors
Haley KE,Kronenberg NM,Liehm P,Elshani M,Bell C,Harrison DJ,Gather MC,Reynolds PAdoi
10.1126/sciadv.aap8030subject
Has Abstractpub_date
2018-06-27 00:00:00pages
eaap8030issue
6issn
2375-2548pii
aap8030journal_volume
4pub_type
杂志文章相关文献
Science Advances文献大全abstract::Colloidal-scale assemblies that reconfigure on demand may serve as the next generation of soft "microbots," artificial muscles, and other biomimetic devices. This requires the precise arrangement of particles into structures that are preprogrammed to reversibly change shape when actuated by external fields. The design...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1701108
更新日期:2017-08-04 00:00:00
abstract::In May 2014, the Balkans were hit by a Vb-type cyclone that brought disastrous flooding and severe damage to Bosnia and Herzegovina, Serbia, and Croatia. Vb cyclones migrate from the Mediterranean, where they absorb warm and moist air, to the north, often causing flooding in central/eastern Europe. Extreme rainfall ev...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1501428
更新日期:2016-04-15 00:00:00
abstract::Modeling a complex system is almost invariably a challenging task. The incorporation of experimental observations can be used to improve the quality of a model and thus to obtain better predictions about the behavior of the corresponding system. This approach, however, is affected by a variety of different errors, esp...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1501177
更新日期:2016-01-22 00:00:00
abstract::Double seismic zones are two-layered distributions of intermediate-depth earthquakes that provide insight into the thermomechanical state of subducting slabs. We present new precise hypocenters of intermediate-depth earthquakes in the Tonga subduction zone obtained using data from local island-based, ocean-bottom, and...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1601755
更新日期:2017-01-11 00:00:00
abstract::The search for topological superconductors (TSCs) is one of the most urgent contemporary problems in condensed matter systems. TSCs are characterized by a full superconducting gap in the bulk and topologically protected gapless surface (or edge) states. Within each vortex core of TSCs, there exists the zero-energy Maj...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1600894
更新日期:2016-11-23 00:00:00
abstract::The human transfer RNA methyltransferase 9-like gene (TRM9L, also known as KIAA1456) encodes a negative regulator of tumor growth that is frequently silenced in many forms of cancer. While TRM9L can inhibit tumor cell growth in vivo, the molecular mechanisms underlying the tumor inhibition activity of TRM9L are unknow...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aas9184
更新日期:2018-07-11 00:00:00
abstract::Functionally graded materials (FGMs) enable applications in fields such as biomedicine and architecture, but their fabrication suffers from shortcomings in gradient continuity, interfacial bonding, and directional freedom. In addition, most commercial design software fail to incorporate property gradient data, hinderi...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aay0929
更新日期:2020-02-21 00:00:00
abstract::Nanomaterials often surprise us with unexpected phenomena. Here, we report a discovery of the anti-twinning deformation, previously thought impossible, in nanoscale body-centered cubic (BCC) tungsten crystals. By conducting in situ transmission electron microscopy nanomechanical testing, we observed the nucleation and...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aay2792
更新日期:2020-06-03 00:00:00
abstract::Interactions between top-down and bottom-up processes in the cerebral cortex hold the key to understanding attentional processes, predictive coding, executive control, and a gamut of other brain functions. However, the underlying circuit mechanism remains poorly understood and represents a major challenge in neuroscie...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1601335
更新日期:2016-11-16 00:00:00
abstract::Two-dimensional (2D) polymers hold great promise in the rational materials design tailored for next-generation applications. However, little is known about the grain boundaries in 2D polymers, not to mention their formation mechanisms and potential influences on the material's functionalities. Using aberration-correct...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abb5976
更新日期:2020-08-14 00:00:00
abstract::Changes in the Atlantic Meridional Overturning Circulation (AMOC) are moderate in most climate model projections under increasing greenhouse gas forcing. This intermodel consensus may be an artifact of common model biases that favor a stable AMOC. Observationally based freshwater budget analyses suggest that the AMOC ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1601666
更新日期:2017-01-04 00:00:00
abstract::Local thermal magnetization fluctuations in Li-doped MnTe are found to increase its thermopower α strongly at temperatures up to 900 K. Below the Néel temperature (TN ~ 307 K), MnTe is antiferromagnetic, and magnon drag contributes αmd to the thermopower, which scales as ~T3. Magnon drag persists into the paramagnetic...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aat9461
更新日期:2019-09-13 00:00:00
abstract::Adaptation is a fundamental property of sensory systems that can change subjective experiences in the context of recent information. Adaptation has been postulated to arise from recurrent circuit mechanisms or as a consequence of neuronally intrinsic suppression. However, it is unclear whether intrinsic suppression by...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abd4205
更新日期:2020-10-14 00:00:00
abstract::Focal adjustment and zooming are universal features of cameras and advanced optical systems. Such tuning is usually performed longitudinally along the optical axis by mechanical or electrical control of focal length. However, the recent advent of ultrathin planar lenses based on metasurfaces (metalenses), which opens ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aap9957
更新日期:2018-02-23 00:00:00
abstract::Polyphosphate, an energy-rich polymer conserved in all kingdoms of life, is integral to many cellular stress responses, including nutrient deprivation, and yet, the mechanisms that underlie its biological roles are not well understood. In this work, we elucidate the physiological function of this polymer in the acclim...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abb5351
更新日期:2020-09-30 00:00:00
abstract::Plasmonic metasurfaces are a promising route for flat panel display applications due to their full color gamut and high spatial resolution. However, this plasmonic coloration cannot be readily tuned and requires expensive lithographic techniques. Here, we present scalable electrically driven color-changing metasurface...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaw2205
更新日期:2019-05-10 00:00:00
abstract::Reef-building corals form their calcium carbonate skeletons within an extracellular calcifying medium (ECM). Despite the critical role of the ECM in coral calcification, ECM carbonate chemistry is poorly constrained in vivo, and full ECM carbonate chemistry has never been characterized based solely on direct in vivo m...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau7447
更新日期:2019-01-16 00:00:00
abstract::Conjugated polymer-polyelectrolyte blends combine and couple electronic semiconductor functionality with selective ionic transport, making them attractive as the active material in organic biosensors and bioelectronics, electrochromic displays, neuromorphic computing, and energy conversion and storage. Although extens...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aao3659
更新日期:2017-12-15 00:00:00
abstract::V1-ATPase is an ATP-driven rotary motor that is composed of a ring-shaped A3B3 complex and a central DF shaft. The nucleotide-free A3B3 complex of Enterococcus hirae, composed of three identical A1B1 heterodimers, showed a unique asymmetrical structure, probably due to the strong binding of the N-terminal barrel domai...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau8149
更新日期:2019-01-30 00:00:00
abstract::Modern tropical forests harbor an enormous diversity of squamates, but fossilization in such environments is uncommon and little is known about tropical lizard assemblages of the Mesozoic. We report the oldest lizard assemblage preserved in amber, providing insight into the poorly preserved but potentially diverse mid...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1501080
更新日期:2016-03-04 00:00:00
abstract::Copper-containing nitrite reductases (CuNiRs), encoded by nirK gene, are found in all kingdoms of life with only 5% of CuNiR denitrifiers having two or more copies of nirK Recently, we have identified two copies of nirK genes in several α-proteobacteria of the order Rhizobiales including Bradyrhizobium sp. ORS 375, en...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abd8523
更新日期:2021-01-01 00:00:00
abstract::The transcriptional repressors Polycomb repressive complex 1 (PRC1) and PRC2 are required to maintain cell fate during embryonic development. PRC1 and PRC2 catalyze distinct histone modifications, establishing repressive chromatin at shared targets. How PRC1, which consists of canonical PRC1 (cPRC1) and variant PRC1 (...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax5692
更新日期:2020-04-01 00:00:00
abstract::We have developed a way to map brain-wide networks using focal pulsed infrared neural stimulation in ultrahigh-field magnetic resonance imaging (MRI). The patterns of connections revealed are similar to those of connections previously mapped with anatomical tract tracing methods. These include connections between cort...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau7046
更新日期:2019-04-24 00:00:00
abstract::Peripheral blood mononuclear cells (PBMCs) may provide insight into the pathogenesis of Alzheimer's disease (AD) or Parkinson's disease (PD). We investigated PBMC samples from 132 well-characterized research participants using seven canonical immune stimulants, mass cytometric identification of 35 PBMC subsets, and si...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abd5575
更新日期:2020-11-25 00:00:00
abstract::Hybrid organic-inorganic halide perovskites have shown remarkable optoelectronic properties, exhibiting an impressive tolerance to defects believed to originate from correlated motion of charge carriers and the polar lattice forming large polarons. Few experimental techniques are capable of directly probing these corr...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaw5558
更新日期:2019-05-31 00:00:00
abstract::A sulfide-oxidizing microorganism, Desulfurivibrio alkaliphilus (DA), generates a consistent enrichment of sulfur-34 (34S) in the produced sulfate of +12.5 per mil or greater. This observation challenges the general consensus that the microbial oxidation of sulfide does not result in large 34S enrichments and suggests...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaw1480
更新日期:2019-07-24 00:00:00
abstract::Hydroxyapatite (HA) has been widely applied in bone repair because of its superior biocompatibility. Recently, a proliferation-suppressive effect of HA nanoparticles (n-HA) against various cancer cells was reported. This study was aimed at assessing the translational value of n-HA both as a bone-regenerating material ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax6946
更新日期:2019-08-02 00:00:00
abstract::To achieve global elimination of hepatitis C virus (HCV), an effective cross-genotype vaccine is needed. The HCV envelope glycoprotein E2 is the main target for neutralizing antibodies (nAbs), which aid in HCV clearance and protection. E2 is structurally flexible and functions in engaging host receptors. Many nAbs bin...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abb5642
更新日期:2020-07-24 00:00:00
abstract::In mammals, the daily rhythms of physiological functions are timed by the central circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. Although the importance of the SCN for the regulation of sleep/wakefulness has been suggested, little is known about the neuronal projections from the SCN,...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abd0384
更新日期:2020-11-06 00:00:00
abstract::Recently, the complexity behind manipulations of reflected fields by metasurfaces has been addressed, showing that, even in the simplest scenarios, nonlocal response and excitation of auxiliary evanescent fields are required for perfect field control. In this work, we introduce purely local reflective metasurfaces for...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau7288
更新日期:2019-02-15 00:00:00