Abstract：
:Since most antibacterial coatings reported to fight biomaterial-associated infections (BAI) fail in completely preventing bacterial colonization, it is crucial to know the impact of that small fraction of adhered bacteria in BAI recrudescence. This study aims to understand the fate of Staphylococcus aureus able to adhere to an antimicrobial coating previously developed, in terms of potential development of bacterial resistance and their macrophage-mediated phagocytosis. Antimicrobial coating comprised the co-immobilization of Palm peptide and DNase I onto polydimethylsiloxane. Expression of genes associated to resistance and virulence mechanisms showed that cells in contact with antimicrobial surfaces for a long period of 30 days, exhibit genes equally or less expressed, as compared to cells recovered from control surfaces. Recovered cells also exhibit the same susceptibility patterns, which strengthens the evidence of no resistance development. Remarkably, cells adhered to modified surfaces shows a reduced metabolic activity upon vancomycin treatment unlike the cells found on control surfaces, which can be identified as a clinical opportunity for prophylactically administration after implant surgery. Furthermore, results highlight that functionalization of PDMS with Palm and DNase I should not compromise the action of host immune cells. The overall results reinforce the potential of this antimicrobial strategy to fight BAI.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Alves DF,Magalhães AP,Neubauer D,Bauer M,Kamysz W,Pereira MOdoi
10.1016/j.actbio.2018.07.052subject
Has Abstractpub_date
2018-09-15 00:00:00pages
189-197eissn
1742-7061issn
1878-7568pii
S1742-7061(18)30455-0journal_volume
78pub_type
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doi：10.1016/j.actbio.2017.08.036
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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journal_title：Acta biomaterialia
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