Abstract:
:Restoration of the external ear and nose in human patients, in either congenital deformity or acquired defects, is a challenge in reconstructive surgery. Optimization of the currently available materials is necessary for rhinoplasty and microtia correction to avoid intraoperative manoeuvring and early rejection. The aim of this study was to develop cross-linked decellularized caprine conchal cartilages as biocompatible, robust, and non-toxic matrix template. The characterization of the decellularized tissue encompasses in vitro lymphoproliferation assay, cytotoxicity test, agar gel precipitation test, in vivo immunocompatibility study, histology, and determination of pro-inflammatory cytokines in animal model. Decellularized cartilage was implanted in human volunteer at R. G. Kar Medical College and Hospital, Kolkata, India, and samples were assessed histologically by retrieving those after 4 months. The processed cartilages were implanted in rhinoplasty (nine) and microtia patients (six) keeping autogenous cartilage graft as control up to 18 months after surgery. Primary outcomes were viability and safety of the material, both in animal model and human pre-application in actual site. Secondary outcomes included self-assessed clinical findings on gross examination. This study is under the ethical approval no. RKC/14 dated January 27, 2012. The in vitro cellular reactivity was less in processed cartilage protein than control. Histology of retrieved tissues in animal model and human volunteer showed no adverse reactions. Production of IL-2, IL-6, and TNF-α cytokines was lower at 4 weeks. The rhinoplasty and microtia operation in clinical patients utilizing the processed cartilage showed satisfactory recovery with improved facial look. These low cost, easily available, biocompatible, safe xenocartilage biomatrices of caprine conchal cartilage origin are very flexible in shape and size, enabling them as potential bioimplant for repair of nasal and auricular structure without any rejection or diverse biomedical applications.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Bhattacharya R,Das P,Joardar SN,Biswas BK,Batabyal S,Das PK,Nandi SKdoi
10.1002/term.2767subject
Has Abstractpub_date
2019-01-01 00:00:00pages
46-57issue
1eissn
1932-6254issn
1932-7005journal_volume
13pub_type
临床试验,杂志文章abstract::The demineralized bone matrix (DBM) is the most widely used bone allograft, which is obtained by removing the mineral component of bone, leading to exposure of the proteins responsible for osteoinduction. For clinical use, DBM shall be formulated with a carrier that provides consistency and improves its osteoinduction...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3012
更新日期:2020-03-01 00:00:00
abstract::In the context of prevascularization strategies for tissue-engineering purposes, co-culture systems consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs) have been established as a promising in vitro tool to study regeneration mechanisms and to identify factors that might positively influence...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2475
更新日期:2018-03-01 00:00:00
abstract::Manufacture of red blood cells (RBCs) from progenitors has been proposed as a method to reduce reliance on donors. Such a process would need to be extremely efficient for economic viability given a relatively low value product and high (2 × 1012 ) cell dose. Therefore, the aim of these studies was to define the produc...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2337
更新日期:2018-01-01 00:00:00
abstract::The authors previously created HAp or CaCO(3) formed on or in agarose gels (HAp and CaCO(3) gels, respectively) as biocompatible and biodegradable bone graft materials. However, these gels have limitations for bone regeneration. Mesenchymal stromal cells (MSCs) have osteogenic potential and are considered useful for b...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.495
更新日期:2013-01-01 00:00:00
abstract::Bone marrow (BM) multipotent mesenchymal stromal cells (MSCs) present with multipotent differentiation potential and immunomodulatory properties. As an alternative to bone marrow, we have examined fetal membranes, amnion and chorion, of term human placenta as a potential source of multipotent MSCs. Here we show that a...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.40
更新日期:2007-07-01 00:00:00
abstract::Treatment of terminal heart failure still poses a significant clinical problem. Cardiac tissue engineering could offer autologous solutions for the replacement of nonfunctional myocardial tissue. So far, soft matrix construction and missing large-scale prevascularization prevented the application of sizeable cardiac r...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3129
更新日期:2020-12-01 00:00:00
abstract::Tendon healing still represents a challenge for clinicians because it is slow and incomplete. The most injured is the Achilles tendon, and surgery is the therapeutic strategy often adopted because it provides a quicker functional recovery. Peritendinous adhesions are the main complication of surgery with hyperplasia a...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3085
更新日期:2020-08-01 00:00:00
abstract::The aim of this study was to establish the potential of human periosteum-derived cells from elderly patients as a cell source for cartilage tissue engineering by optimizing culture conditions for both proliferation and differentiation. Periosteum was obtained from the tibiae of nine patients. Biopsies were prepared fo...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.100
更新日期:2008-08-01 00:00:00
abstract::The aim of this study was to investigate the effects of a novel bioactive material (Biosilicate®) and low-level laser therapy (LLLT) on bone fracture consolidation in osteoporotic rats. Forty female Wistar rats were submitted to ovariectomy (OVX) to induce osteopenia. Eight weeks after surgery, the animals were random...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.309
更新日期:2011-03-01 00:00:00
abstract::In orthopaedic medicine, connective tissues are often affected by traumatic or degenerative injuries, and surgical intervention is required. Rotator cuff tears are a common cause of shoulder pain and disability among adults. The development of graft materials for bridging the gap between tendon and bone after chronic ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2868
更新日期:2019-07-01 00:00:00
abstract::The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.2597
更新日期:2018-03-01 00:00:00
abstract::Healing of diabetic foot ulcers is a major challenge. Despite adhering to optimal standard of care (SOC), less than 30% of wounds heal after 20 weeks. Advanced cellular tissue-based products have shown better healing over SOC, albeit with great cost and modest improvement. We hypothesized no difference in healing effe...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/term.2884
更新日期:2019-08-01 00:00:00
abstract::The objective of this preliminary study was to evaluate the applying of chitosan (CS)-based microparticles (MPs) in bone regeneration in vivo. The CS MPs were fabricated using our scale-up method, as previously described. Mesenchymal stem cells (MSC) were harvested from the femora and tibiae of Dark Agouti (DA) rats a...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.270
更新日期:2010-10-01 00:00:00
abstract::Injections of bone marrow (BM) cell extract, known as 'BM soup', were previously reported to mitigate ionizing radiation (IR) injury to salivary glands (SGs). However, the optimal starting time and frequency to maintain BM soup therapeutic efficacy remains unknown. This study tested the optimal starting time and frequ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2513
更新日期:2018-02-01 00:00:00
abstract::Nonvascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of fat in the host environment remains a problem. A process known as cell-assisted transfer is used to enhance fat graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content t...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2463
更新日期:2018-02-01 00:00:00
abstract::Prevascularization is essential to ensure the viability, functionality, and successful integration of tissue-engineered three-dimensional (3D) constructs with surrounding host tissues after transplantation. Human mesenchymal stem cell (hMSC) sheet can be prevascularized by coculturing with endothelial cells (ECs), and...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3167
更新日期:2021-01-11 00:00:00
abstract::'Off-the-shelf' tissue-engineered skin alternatives for epidermal and dermal skin layers are available; however, no such alternative for the subdermal fat layer exists. Without this well-vascularized layer, skin graft take is variable and grafts may have reduced mobility, contracture and contour defects. In this study...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2025
更新日期:2017-04-01 00:00:00
abstract::Wound healing remains a major challenge in modern medicine. Bone marrow- (BM) and adipose tissue- (AT) derived mesenchymal stromal/stem cells (MSCs) are of great interest for tissue reconstruction due to their unique immunological properties and regenerative potential. The purpose of this study was to characterize BM ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1700
更新日期:2016-02-01 00:00:00
abstract::Research in regenerative medicine is developing at a significantly quick pace. Cell-based bone and cartilage replacement is an evolving therapy aiming at the treatment of patients who suffer from limb amputation, damaged tissues and various bone and cartilage-related disorders. Stem cells are undifferentiated cells wi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.173
更新日期:2009-07-01 00:00:00
abstract::Surface-modified titanium (Ti) samples with hydroxyapatite (HAp) and heparin (Hep)-bone morphogenetic protein-2 (BMP-2) complex (Ti/HAp/Hep/BMP-2) were prepared, and their efficacies on the enhancements of bone formation and osseointegration in vitro and in vivo were examined as compared to Ti/HAp and Ti/Hep/BMP-2. Th...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1973
更新日期:2015-09-01 00:00:00
abstract::Nonunion treatment has a high rate of success, although recalcitrant nonunion may determine the need for amputation. Therefore, new treatment options are continuously investigated in order to further reduce the risk of nonunion recurrence. This study aimed to (a) develop a new large animal model for bone atrophic nonu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2732
更新日期:2018-09-01 00:00:00
abstract::In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration. In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2241
更新日期:2017-01-01 00:00:00
abstract::Several techniques have been introduced to improve the pulp revascularization outcomes. The use of the tissue graft can create more practical tissue regeneration, provide vascular supply, and enhance tissue healing. The aim of the present study was to investigate the histologic and molecular outcomes of pulp revascula...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3094
更新日期:2020-09-01 00:00:00
abstract::Dermal papilla cells (DPCs) interact with epithelial stem cells and induce hair folliculogenesis. Cell-based therapies using expanded DPCs for hair regeneration have been unsuccessful in humans. Two major challenges remain: first, expanded DPCs obtained from adult hair follicles have functional limitations; second, a ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.400
更新日期:2012-02-01 00:00:00
abstract::The composition and organization of the vocal fold extracellular matrix (ECM) provide the viscoelastic mechanical properties that are required to sustain high-frequency vibration during voice production. Although vocal injury and pathology are known to produce alterations in matrix physiology, the mechanisms responsib...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.219
更新日期:2010-01-01 00:00:00
abstract::To establish an ex vivo expansion method of haematopoietic progenitor cells (HPCs) and erythroid cells, three-dimensional (3D) cultures of mouse bone marrow cells were performed, employing a porous polyvinyl formal (PVF) resin as a scaffold. In these cultures, the effects of oxygen concentration and co-cultures with s...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.295
更新日期:2011-02-01 00:00:00
abstract::Over the last few years, the generation of induced pluripotent stem cells (iPSCs) from human somatic cells has proved to be one of the most potentially useful discoveries in regenerative medicine. iPSCs are becoming an invaluable tool to study the pathology of different diseases and for drug screening. However, severa...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.2021
更新日期:2016-11-01 00:00:00
abstract::We recently showed the fortifying effect of poly-caprolactone (PCL) scaffold in liquid solder-mediated laser-assisted vascular repair (ssLAVR) of porcine carotid arteries, yielding a mean ± SD leaking point pressure of 488 ± 111 mmHg. Despite supraphysiological pressures, the frequency of adhesive failures was indicat...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.486
更新日期:2012-11-01 00:00:00
abstract::Poor angiogenesis within tissue-engineered grafts has been identified as a main challenge limiting the clinical introduction of bone tissue-engineering (BTE) approaches for the repair of large bone defects. Thick BTE grafts often exhibit poor cellular viability particularly at the core, leading to graft failure and la...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1617
更新日期:2015-02-01 00:00:00
abstract::Intervertebral disc (IVD) degeneration has been described as an aberrant, cell-mediated, age- and genetics-dependent molecular degeneration process, which can be accelerated by nutritional, mechanical and toxic factors. Collective involvement of these factors can result in structural failures, which are often associat...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1583
更新日期:2014-12-01 00:00:00