Abstract:
:'Off-the-shelf' tissue-engineered skin alternatives for epidermal and dermal skin layers are available; however, no such alternative for the subdermal fat layer exists. Without this well-vascularized layer, skin graft take is variable and grafts may have reduced mobility, contracture and contour defects. In this study a novel adipose-derived acellular matrix (Adipogel) was investigated for its properties to generate subdermal fat in a rat model. In a dorsal thoracic site, a 1 × 1 cm Adipogel implant was inserted within a subdermal fat layer defect. In a dorsal lumbar site, an Adipogel implant was inserted in a subfascial pocket. Contralateral control defects remained empty. At 8 weeks wound/implant sites were evaluated histologically, immunohistochemically and morphometrically. Identifiable thoracic Adipogel implants lost volume in vivo over 8 weeks. Neovascularization and adipogenesis were evident within implants and adipocyte percentage volume was 33.07 ± 6.55% (mean ± SEM). A comparison of entire cross-sections of thoracic wounds demonstrated a significant increase in total wound fat in Adipogel-implanted wounds (37.19 ± 4.48%, mean ± SEM) compared to control (16.53 ± 4.60%; p = 0.0092), indicating that some Adipogel had been completely converted to normal fat. At the lumbar site, Adipogel also lost volume, appearing flattened, although fat generation and angiogenesis occurred. At both sites macrophage infiltration was mild, whilst many infiltrating cells were PDGFRβ-positive mesenchymal cells. Adipogel is adipogenic and angiogenic and is a promising candidate for subcutaneous fat regeneration; it has the potential to be a valuable adjunct to wound-healing therapy and reconstructive surgery practice. Copyright © 2015 John Wiley & Sons, Ltd.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Debels H,Gerrand YW,Poon CJ,Abberton KM,Morrison WA,Mitchell GMdoi
10.1002/term.2025subject
Has Abstractpub_date
2017-04-01 00:00:00pages
1230-1241issue
4eissn
1932-6254issn
1932-7005journal_volume
11pub_type
杂志文章abstract::Postoperative mucosal regeneration of the middle ear cavity and the mastoid cavity is of great importance after middle ear surgery. However, the epithelialization of the mucosa in the middle ear is retarded because chronic inflammation without epithelialization aggravates gas exchange and clinical function. These envi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2012
更新日期:2017-04-01 00:00:00
abstract::Fracture haematoma formation is the first and foremost important stage of fracture healing. It orchestrates the inflammatory and cellular processes leading to the formation of callus and the restoration of the continuity of the bone. Evidence suggests that blocking this initial stage could lead to an impairment of the...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.2593
更新日期:2018-03-01 00:00:00
abstract::In this study, a new foaming method, based on physical foaming combined with microwave-induced curing, is proposed in combination with a surface bioactivation to develop scaffold for bone tissue regeneration. In the first step of the process, a stable physical foaming was induced using a surfactant (Pluronic) as blowi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2241
更新日期:2017-01-01 00:00:00
abstract::The availability of autologous articular chondrocytes remains a limiting issue in matrix assisted autologous chondrocyte transplantation. Non-articular heterotopic chondrocytes could be an alternative autologous cell source. The aims of this study were to establish heterotopic chondrocyte cocultures to analyze cell-ce...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.496
更新日期:2013-01-01 00:00:00
abstract::For the success of any biomaterial for tissue engineering, its mechanical properties and ability to support nutrient diffusion will be critical. Collagen scaffolds are ideal candidates, due to their ability to immerse cells in a biomimetic nanofibrous matrix. We have established O(2) diffusion coefficients through nat...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.402
更新日期:2012-01-01 00:00:00
abstract::The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1848
更新日期:2015-08-01 00:00:00
abstract::Organ transplantation is an effective treatment for chronic organ dysfunctioning conditions. However, a dearth of available donor organs for transplantation leads to the death of numerous patients waiting for a suitable organ donor. The potential of decellularized scaffolds, derived from native tissues or organs in th...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.2061
更新日期:2017-04-01 00:00:00
abstract::The osteo-regenerative properties of allograft have recently been enhanced by addition of autogenous human bone marrow stromal cells (HBMSCs). Limitations in the use of allograft have prompted the investigation of tantalum trabecular metal (TTM) as a potential alternative. TTM is already in widespread orthopaedic use,...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1525
更新日期:2014-04-01 00:00:00
abstract::Self-sustainability after implantation is one of the critical obstacles facing large engineered tissues. A preformed functional vascular network provides an effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered t...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2512
更新日期:2018-03-01 00:00:00
abstract::Tissue-engineering strategies may be employed in the development of in vitro breast tissue models for use in testing regimens of drug therapies and vaccines. The physical and chemical interactions that occur among cells and extracellular matrix components can also be elucidated with these models to gain an understandi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.162
更新日期:2009-06-01 00:00:00
abstract::In the context of prevascularization strategies for tissue-engineering purposes, co-culture systems consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs) have been established as a promising in vitro tool to study regeneration mechanisms and to identify factors that might positively influence...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2475
更新日期:2018-03-01 00:00:00
abstract::The objective was to improve proliferation and chondrogenic potential of adipose stem cells (ASCs) by expansion on extracellular matrix (ECM) deposited by either ASCs or synovium-derived stem cells (SDSCs). ASCs isolated from porcine infrapatellar fat pad were separately expanded on conventional plastic flasks, ASC-de...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.505
更新日期:2013-01-01 00:00:00
abstract::The ability to control facial skin physiology and appearance through the oral mucosa (transbuccally) is largely unexplored. Here, a hypothesis was tested that transbuccal delivery of fat tissue-supportive actives may trigger beneficial cosmetic responses at the level of the skin. First, the importance of the fat tissu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type:
doi:10.1002/term.3087
更新日期:2020-08-01 00:00:00
abstract::Tissue engineering of skeletal muscle is an encouraging possibility for the treatment of muscle loss through the creation of functional muscle tissue in vitro from human stem cells. Currently, the preferred stem cells are primary, non-immunogenic satellite cells ( = myoblasts). The objective of this study was to deter...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.417
更新日期:2011-08-01 00:00:00
abstract::Transplantation of mesenchymal stem cells (MSCs) has been proposed to exert beneficial effects on peripheral nerve regeneration after a peripheral nerve injury, but the functional recovery in the denervated limb is still limited. In this study, we used low-level laser therapy (LLLT) as an adjunct therapy for MSC trans...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1714
更新日期:2016-02-01 00:00:00
abstract::The aim of this study was to establish the potential of human periosteum-derived cells from elderly patients as a cell source for cartilage tissue engineering by optimizing culture conditions for both proliferation and differentiation. Periosteum was obtained from the tibiae of nine patients. Biopsies were prepared fo...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.100
更新日期:2008-08-01 00:00:00
abstract::Cardiac stem cells (CSCs) represent a logical cell type to exploit as a regenerative treatment option for tissue damage accrued as a result of a myocardial infarction. However, the isolation and expansion of CSCs prior to cell transplantation is time consuming, costly and invasive, and the reliability of cell expansio...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2392
更新日期:2018-01-01 00:00:00
abstract::The composition and organization of the vocal fold extracellular matrix (ECM) provide the viscoelastic mechanical properties that are required to sustain high-frequency vibration during voice production. Although vocal injury and pathology are known to produce alterations in matrix physiology, the mechanisms responsib...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.219
更新日期:2010-01-01 00:00:00
abstract::Intervertebral disc (IVD) degeneration has been described as an aberrant, cell-mediated, age- and genetics-dependent molecular degeneration process, which can be accelerated by nutritional, mechanical and toxic factors. Collective involvement of these factors can result in structural failures, which are often associat...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1583
更新日期:2014-12-01 00:00:00
abstract::Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been considered to be a promising strategy for wound healing. However, poor viability of engrafted BMSCs and limited capabilities of differentiation into the desired cell types in wounds often hinder its application. Few studies report the induction of ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2400
更新日期:2018-02-01 00:00:00
abstract::Our knowledge of the immunomodulatory role of mesenchymal stem cells (MSCs) in both the innate and adaptive immune systems has dramatically expanded, providing great promise for treating various autoimmune diseases. However, the contribution of MSCs to Th17-dominant immune disease, such as psoriasis and its underlying...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2423
更新日期:2018-02-01 00:00:00
abstract::Nanopatterned (330 nm wide channels) type I collagen films were prepared by solvent casting on poly(dimethyl siloxane) (PDMS) templates. These films were rolled into tubular constructs and crosslinked. Tubular constructs were incubated under cell culture conditions for 28 days and examined by stereomicroscopy and scan...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.99
更新日期:2008-08-01 00:00:00
abstract::The creation of vascularized engineered tissues of clinically relevant size is a major challenge of tissue engineering. While it is known that endothelial and mural vascular cells are integral to the formation of stable blood vessels, the specific cell types and optimal conditions for engineered vascular networks are ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.231
更新日期:2010-03-01 00:00:00
abstract::Damage of non-vascularised tissues such as cartilage and cornea can result in healing processes accompanied by a non-physiological angiogenesis. Peptidic aptamers have recently been reported to block the vascular endothelial growth factor (VEGF). However, the therapeutic applications of these aptamers are limited due ...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2340
更新日期:2018-02-01 00:00:00
abstract::Despite the high prevalence of skin conditions in the horse, there is a dearth of literature on the culture and biology of equine skin cells, and this is partially attributable to the lack of suitable in vitro skin models. The objective of this study was to develop a three-dimensional (3D) culture system that would su...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1788
更新日期:2016-08-01 00:00:00
abstract::In clinical cases, many orthopaedists have been troubled with bone fragility, such as fractures after devitalization therapy for bone tumour, pathological fractures and metastatic tumours. The aim of this study was to determine whether loss of osteogenic capacity of devitalized autologous bones can be rescued using cu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.67
更新日期:2008-01-01 00:00:00
abstract::The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.2597
更新日期:2018-03-01 00:00:00
abstract::Enhancement of osteogenic capacity was achieved in a mineralized collagen composite, nano-hydroxyapatite/collagen (nHAC), by loading with synthetic peptides derived from BMP-2 residues 32-48 (P17-BMP-2). Rabbit marrow stromal cells (MSCs) were used in vitro to study cell biocompatibility, attachment and differentiatio...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1705
更新日期:2016-02-01 00:00:00
abstract::The combination of scaffolds and mesenchymal stromal cells (MSCs) is a promising approach in bone tissue engineering (BTE). Knowledge on the survival, outgrowth and bone-forming capacity of MSCs in vivo is limited. Bioluminescence imaging (BLI), histomorphometry and immunohistochemistry were combined to study the fate...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1681
更新日期:2016-03-01 00:00:00
abstract::Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have desc...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2249
更新日期:2017-12-01 00:00:00