Abstract:
:We recently showed the fortifying effect of poly-caprolactone (PCL) scaffold in liquid solder-mediated laser-assisted vascular repair (ssLAVR) of porcine carotid arteries, yielding a mean ± SD leaking point pressure of 488 ± 111 mmHg. Despite supraphysiological pressures, the frequency of adhesive failures was indicative of weak bonding at the solder-tissue interface. As a result, this study aimed to improve adhesive bonding by using a semi-solid solder and single-spot vs. scanning irradiation. In the first experiment, in vitro ssLAVR (n=30) was performed on porcine abdominal aorta strips using a PCL scaffold with a liquid or semi-solid solder and a 670-nm diode laser for dual-pass scanning. In the second experiment, the scanning method was compared to single-spot lasing. The third experiment investigated the stability of the welds following hydration under quasi-physiological conditions. The welding strength was defined by acute breaking strength (BS). Solder-tissue bonding was examined by scanning electron microscopy and histological analysis was performed for thermal damage analysis. Altering solder viscosity from liquid to semi-solid solder increased the BS from 78 ± 22 N/cm(2) to 131 ± 38 N/cm(2) . Compared to scanning ssLAVR, single-spot lasing improved adhesive bonding to a BS of 257 ± 62 N/cm(2) and showed fewer structural defects at the solder-tissue interface but more pronounced thermal damage. The improvement in adhesive bonding was associated with constantly stronger welds during two weeks of hydration. Semi-solid solder and single-spot lasing increased welding strength by reducing solder leakage and improving adhesive bonding, respectively. The improvement in adhesive bonding was associated with enhanced weld stability during hydration.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Pabittei DR,Heger M,Simonet M,van Tuijl S,van der Wal AC,Beek JF,Balm R,de Mol BAdoi
10.1002/term.486subject
Has Abstractpub_date
2012-11-01 00:00:00pages
803-12issue
10eissn
1932-6254issn
1932-7005journal_volume
6pub_type
杂志文章abstract::Tissue engineering applications require an appropriate combination of a cell population, biochemical factors and scaffold materials. In this field, mesenchymal stem cells (MSCs) emerge as an attractive cell population, due to their ready availability and their potential to be differentiated into various mesodermal cel...
journal_title:Journal of tissue engineering and regenerative medicine
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pub_type: 临床试验,杂志文章
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2012-10-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1734
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2092
更新日期:2017-07-01 00:00:00
abstract::Adipose stem cells (ASCs) represent a cell population with great potential for tissue engineering applications. Several articles have been published showing the proliferation and differentiation potential, the markers and the wide range of potential applications of these cells. In the majority of these studies the ASC...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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doi:10.1002/term.328
更新日期:2011-05-01 00:00:00
abstract::The objective was to improve proliferation and chondrogenic potential of adipose stem cells (ASCs) by expansion on extracellular matrix (ECM) deposited by either ASCs or synovium-derived stem cells (SDSCs). ASCs isolated from porcine infrapatellar fat pad were separately expanded on conventional plastic flasks, ASC-de...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.505
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:According to experimental studies, mesh implants may reduce in size up to 50.8% during their integration into soft tissues. This results in impaired mobility of the anterior abdominal wall and hernia recurrences, as well as affects patients' quality of life. Due to unsatisfactory radiographic contrast of pol...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.3029
更新日期:2020-05-01 00:00:00
abstract::Our recent clinical studies have demonstrated that autologous implantation of human cultured periosteal (hCP) sheets in combination with porous hydroxylapatite (HAp) particles at the site of periodontal bone defects strikingly facilitates tissue regeneration. To better understand how the hCP sheet functions at the imp...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.156
更新日期:2009-03-01 00:00:00
abstract::Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant financial costs. We have developed a method for decellularization (DC) based on vacuum techno...
journal_title:Journal of tissue engineering and regenerative medicine
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doi:10.1002/term.1979
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abstract::Limitations of current treatments for intervertebral disc (IVD) degeneration have promoted interest in the development of tissue-engineering approaches. Injectable hydrogels loaded with cells can be used as a substitute material for the inner IVD part, the nucleus pulposus (NP), and provide an opportunity for minimall...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2016-10-01 00:00:00
abstract::Bone-patellar tendon-bone (BPTB) autografts are the gold standard for anterior cruciate ligament (ACL) reconstruction because the bony ends allow for superior healing and anchoring through bone-to-bone regeneration. However, the disadvantages of BPTB grafts include donor site morbidity and patellar rupture. In order t...
journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2017-03-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2017-02-01 00:00:00
abstract::Silk fibroin (SF) hydrogels can be obtained via self-assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol) sodium salt (DMPG), was used to induce and accelerate the gelatio...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
abstract::'Off-the-shelf' tissue-engineered skin alternatives for epidermal and dermal skin layers are available; however, no such alternative for the subdermal fat layer exists. Without this well-vascularized layer, skin graft take is variable and grafts may have reduced mobility, contracture and contour defects. In this study...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2025
更新日期:2017-04-01 00:00:00
abstract::Lipofilling or lipografting is a novel and promising treatment method for reduction or prevention of dermal scars after injury. Ample anecdotal evidence from case reports supports the scar-reducing properties of adipose tissue grafts. However, only a few properly controlled and designed clinical trials have been condu...
journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2017-11-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2013-08-01 00:00:00