Abstract:
:Dermal papilla cells (DPCs) interact with epithelial stem cells and induce hair folliculogenesis. Cell-based therapies using expanded DPCs for hair regeneration have been unsuccessful in humans. Two major challenges remain: first, expanded DPCs obtained from adult hair follicles have functional limitations; second, a clinically applicable method is needed for transplanting DPCs. This study aimed to identify an efficient, minimally invasive and economical DPC transplantation procedure for use in clinical settings. Five clinically applicable transplantation procedures were tested, termed the Pinhole, Laser, Slit, Non-vascularized sandwich (NVS) and Hemi-vascularized sandwich (HVS) methods. Labelled rat dermal papilla tissue was transplanted into rat sole skin, and hair follicle regeneration was evaluated histologically. Regenerated follicles and labelled DPCs were detected for all methods, although some follicles showed abnormal growth, i.e. a cystic or inverted appearance. The HVS method, pioneered here, resulted in significantly larger number of regenerated follicles that were more mature and regular than those observed using the other methods. Moreover, hair growth was detected after expanded adult-derived DPC transplantation using the HVS method. These results suggest that direct contact of epithelial and dermal components and better vascularization/oxygenation of the recipient site are critical for hair regeneration in cell-based therapies.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Aoi N,Inoue K,Kato H,Suga H,Higashino T,Eto H,Doi K,Araki J,Iida T,Katsuta T,Yoshimura Kdoi
10.1002/term.400subject
Has Abstractpub_date
2012-02-01 00:00:00pages
85-95issue
2eissn
1932-6254issn
1932-7005journal_volume
6pub_type
杂志文章abstract::Limitations of current treatments for intervertebral disc (IVD) degeneration have promoted interest in the development of tissue-engineering approaches. Injectable hydrogels loaded with cells can be used as a substitute material for the inner IVD part, the nucleus pulposus (NP), and provide an opportunity for minimall...
journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
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abstract::The effects of mesenchymal stem cells (MSCs) on the phenotype and function of natural killer T (NKT) cells is not understood. We used concanavalin A (Con A) and α-galactosylceramide (α-GalCer)-induced liver injury to evaluate the effects of MSCs on NKT-dependent hepatotoxicity. Mouse MSCs (mMSCs) significantly reduced...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2016-02-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
abstract::Whole body vibration (WBV) is a very widespread mechanical stimulus used in physical therapy, rehabilitation and fitness centres. It has been demonstrated that vibration induces improvements in muscular strength and performance and increases bone density. We investigated the effects of low-amplitude, high frequency vi...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1533
更新日期:2014-05-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2842
更新日期:2019-05-01 00:00:00
abstract::Obtaining an efficient, uniform and reproducible cell seeding of porous tubular scaffolds constitutes a major challenge for the successful development of tissue-engineered vascular grafts. In this study, a novel automated cell-seeding device utilizing direct cell deposition, patterning techniques and scaffold rotation...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
abstract::Chronic proteolytic disruption of elastic fibres within the abdominal aortic wall results in wall vessel expansion to form rupture-prone abdominal aortic aneurysms (AAA). Arresting AAA growth is not possible as adult vascular smooth muscle cells (SMCs) poorly auto-regenerate and repair elastic fibres. Thus, there is a...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2349
更新日期:2018-01-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1905
更新日期:2017-01-01 00:00:00
abstract::In order to examine the differentiation potential of the tenocytes expanded in our defined culture medium (reported previously) and the effect of sequential combination of the two culture conditions on human tenocytes, a two-dimensional and three-dimensional experimental approach was used. Human tenocytes were sequent...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.1791
更新日期:2016-03-01 00:00:00
abstract::The mechanism for stem cell-mediated improvement following acute myocardial infarction has been actively debated. We support hypotheses that the stem cell effect is primarily paracrine factor-linked. We used a heparin-presenting injectable nanofibre network to bind and deliver paracrine factors derived from hypoxic co...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.273
更新日期:2010-12-01 00:00:00
abstract::Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues an...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2092
更新日期:2017-07-01 00:00:00
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更新日期:2009-02-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2018-05-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
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更新日期:2018-03-01 00:00:00
abstract::Macroporous calcium phosphate cement (CPC) with stem cell seeding is promising for bone regeneration. The objective of this study was to investigate the effects of co-delivering autologous bone marrow mesenchymal stem cells (BMSCs) and autologous platelet-rich plasma (PRP) in CPC scaffold for bone regeneration in mini...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
abstract::Axial micromotion of bone fragments promotes callus formation and bone healing during the process of distraction osteogenesis (DO). This study investigated the effects of the combined axial compression and distraction (accordion) technique on bone regeneration in rat DO model. Male Sprague-Dawley rats (n = 62) underwe...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
abstract::The ability to control facial skin physiology and appearance through the oral mucosa (transbuccally) is largely unexplored. Here, a hypothesis was tested that transbuccal delivery of fat tissue-supportive actives may trigger beneficial cosmetic responses at the level of the skin. First, the importance of the fat tissu...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type:
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更新日期:2020-08-01 00:00:00
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journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.331
更新日期:2011-06-01 00:00:00
abstract::Many studies have highlighted the role of silicon in human bone formation and maintenance. Silicon, in fact, is considered to nucleate the precipitation of hydroxyapatite and to reduce the bone resorption. For this reason, we have combined silk fibroin (SF) with silicon-releasing diatom particles (DPs), as potential m...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章
doi:10.1002/term.2373
更新日期:2018-01-01 00:00:00
abstract::Intervertebral disc (IVD) degeneration has been described as an aberrant, cell-mediated, age- and genetics-dependent molecular degeneration process, which can be accelerated by nutritional, mechanical and toxic factors. Collective involvement of these factors can result in structural failures, which are often associat...
journal_title:Journal of tissue engineering and regenerative medicine
pub_type: 杂志文章,评审
doi:10.1002/term.1583
更新日期:2014-12-01 00:00:00
abstract::Heart valve replacement is often the only solution for patients suffering from valvular heart disease. However, currently available valve replacements require either life-long anticoagulation or are associated with valve degeneration and calcification. Moreover, they are suboptimal for young patients, because they do ...
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pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00