Abstract:
PURPOSE:The purpose of this study was to investigate the feasibility of in vivo imaging of human pancreatic ductal cells by OATP1B3 reporter gene under MRI. METHODS:A human cell line (PANC-1) derived from the pancreatic ductal epithelium was used in this study. After transduction of OATP1B3, the cellular physiological functions and the ability of intracellular uptake of the MRI contrast medium (Gd-EOB-DTPA) were examined. Induced differentiation of the PANC-1 cells into hormone-secreting cells were performed to simulate pancreatic β-like cells. The hormone-secreting cells were implanted into rats and in vivo MRI was evaluated. RESULTS:The mRNA and proteins of OATP1B3 were highly expressed. No significant change of cellular physiological functions was found after the expression. After induced differentiation, the hormone secretion capacities of the OATP1B3-expressing PANC-1 cells were confirmed. Intra-cellular uptake of Gd-EOB-DTPA was determined in vitro by inductively coupled plasma mass spectrometry and MRI. In vivo MRI of the OATP1B3-expressing xenograft revealed an increased signal intensity after contrast enhancement. CONCLUSION:OATP1B3 can be used as a safe and feasible in vivo MRI gene reporter for human pancreatic ductal cells.
journal_name
Magn Reson Medjournal_title
Magnetic resonance in medicineauthors
Wu MR,Hsiao JK,Liu HM,Huang YY,Tseng YJ,Chou PT,Weng TI,Yang CYdoi
10.1002/mrm.27749subject
Has Abstractpub_date
2019-08-01 00:00:00pages
763-774issue
2eissn
0740-3194issn
1522-2594journal_volume
82pub_type
杂志文章abstract::Cortical spreading depression (CSD) was visualized using manganese-enhanced MRI (MEMRI) following topical application of KCl to the exposed rat cortex. MEMRI signal increase in the ipsilateral cortex relative to the contralateral control region was 60 +/- 30% following two KCl applications. MEMRI signal increase for a...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.20438
更新日期:2005-04-01 00:00:00
abstract::Four-dimensional (4D) flow imaging has been used to study flow patterns and pathophysiology, usually focused on specific thoracic vessels and cardiac chambers. Whole-heart 4D flow at high measurement accuracy covering the entire thoracic cardiovascular system would be desirable to simplify and improve hemodynamic asse...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22090
更新日期:2009-10-01 00:00:00
abstract:PURPOSE:To validate an automatic algorithm for offline T2* measurements, providing robust, vendor-independent T2*, and uncertainty estimates for iron load quantification in the heart and liver using clinically available imaging sequences. METHODS:A T2* region of interest (ROI)-based algorithm was developed for robustn...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.25767
更新日期:2016-04-01 00:00:00
abstract::This work aimed to investigate the effects of administering Gd-DTPA on the BOLD effect, and to determine the feasibility of using this approach to measure fractional changes in blood volume and blood oxygenation on neuronal activation during a visual paradigm. A linear relationship between cortical R(2)(*) and the int...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.10340
更新日期:2003-01-01 00:00:00
abstract::Subject motion during scan is a major source of artifacts in MR examinations. Prospective motion correction is a promising technique that tracks subject motion and adjusts the imaging volume in real time; however, additional retrospective correction may be necessary to achieve robust image quality and compatibility wi...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24269
更新日期:2013-02-01 00:00:00
abstract::Noise in the composite magnitude signal from multiple-coil systems is usually assumed to follow a noncentral χ distribution when sum of squares is used to combine images sensed at different coils. However, this is true only if the variance of noise is the same for all coils, and no correlation exists between them. We ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.23020
更新日期:2012-02-01 00:00:00
abstract:PURPOSE:To quantify chemical exchange saturation transfer contrast in upper extremities of participants with lymphedema before and after standardized lymphatic mobilization therapy using correction procedures for B0 and B1 heterogeneity, and T1 relaxation. METHODS:Females with (n = 12) and without (n = 17) breast canc...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.28031
更新日期:2020-05-01 00:00:00
abstract::MR techniques have proven their ability to investigate skeletal muscle function in situ. Their benefit in terms of noninvasiveness is, however, lost in animal research, given that muscle stimulation and force output measurements are usually achieved using invasive surgical procedures, thereby excluding repeated invest...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22386
更新日期:2010-07-01 00:00:00
abstract::We describe a versatile NMR probe that is designed for a variety of in vivo spectroscopic studies on small animals in vertical wide-bore magnets. Replaceable brackets enable the coils to be exchanged readily in order to observe 1H, 13C, 31P, and other nuclei, and to carry out double-resonance experiments. Two solenoid...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910060102
更新日期:1988-01-01 00:00:00
abstract:PURPOSE:To develop velocity-selective (VS) MR angiography (MRA) protocols for arteriography and venography with whole-brain coverage. METHODS:Tissue suppression using velocity-selective saturation (VSS) pulse trains is sensitive to radiofrequency field (B1 +) inhomogeneity. To reduce its sensitivity, we replaced the l...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.26864
更新日期:2018-04-01 00:00:00
abstract:PURPOSE:High-resolution intravascular (IV) MRI is susceptible to degradation from physiological motion and requires high frame-rates for true endoscopy. Traditional cardiac-gating techniques compromise efficiency by reducing the effective scan rate. Here we test whether compressed sensing (CS) reconstruction and ungate...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.25436
更新日期:2015-08-01 00:00:00
abstract::Accurate and reproducible MRI R2 * relaxometry for tissue iron quantification is important in managing transfusion-dependent patients. MRI data are often acquired using array coils and reconstructed by the root-sum-square algorithm, and as such, measured signals follow the noncentral chi distribution. In this study, t...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24607
更新日期:2013-12-01 00:00:00
abstract::Dextran-magnetite, a superparamagnetic compound, is a powerful relaxation reagent for sodium. Administered intravenously, it is confined mainly to the vasculature and eliminates the signal from plasma sodium, a significant component of the tissue sodium signal. Applications of dextran-magnetite for in vivo sodium imag...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910080406
更新日期:1988-12-01 00:00:00
abstract::T2 relaxation time is a promising MRI parameter for the detection of cartilage degeneration in osteoarthritis. However, the accuracy and precision of the measured T2 may be substantially impaired by the low signal-to-noise ratio of images available from clinical examinations. The purpose of this work was to assess the...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.22178
更新日期:2010-01-01 00:00:00
abstract::Diffusion tensor imaging has been used in a number of spinal cord studies, but severe distortions caused by susceptibility induced field inhomogeneities limit its applicability to investigate small volumes within acceptable acquisition times. A way to evaluate image distortions is to map the point spread function of t...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24235
更新日期:2013-01-01 00:00:00
abstract::Atherosclerosis initially develops predominantly at the aortic root and carotid origin, where effective visualization in mice requires efficient cardiac and respiratory gating. The present study sought to first compare the high-resolution MRI gating performance of two digital gating strategies using: 1) separate cardi...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.21308
更新日期:2007-12-01 00:00:00
abstract::Advances in modern magnetic resonance imaging (MRI) pulse sequences have enabled clinically practical cortical bone imaging. Human cortical bone is known to contain a distribution of T(1) and T(2) components attributed to bound and pore water, although clinical imaging approaches have yet to discriminate bound from po...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.24186
更新日期:2012-12-01 00:00:00
abstract:PURPOSE:To characterize the q-space truncation and sampling on the spin-displacement probability density function (PDF) in diffusion spectrum imaging (DSI). METHODS:DSI data were acquired using the MGH-USC connectome scanner (Gmax = 300 mT/m) with bmax = 30,000 s/mm2 , 17 × 17 × 17, 15 × 15 × 15 and 11 × 11 × 11 gri...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.26071
更新日期:2016-12-01 00:00:00
abstract::The experiments reported herein are the first MRI investigations of the orientational dependence of T(2) relaxation in articular cartilage at microscopic resolution over the 360 degrees angular space. For each of six canine cartilage specimens, 48 independent T(2)-weighted proton images were acquired for 12 different ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.10216
更新日期:2002-09-01 00:00:00
abstract::We have obtained high-field (11.7 Tesla), high-resolution carbon-13 solid-state "magic-angle" sample-spinning nuclear magnetic resonance (NMR) spectra of a variety of phospholipids, sphingolipids, myelin and white matter samples, resolving and assigning over 40 resonances in the spectra of human and bovine myelin. The...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910290204
更新日期:1993-02-01 00:00:00
abstract:PURPOSE:Recent literature shows that diffusion tensor properties can be estimated more accurately with diffusion kurtosis imaging (DKI) than with diffusion tensor imaging (DTI). Furthermore, the additional non-Gaussian diffusion features from DKI can be sensitive markers for tissue characterization. Despite these benef...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.25165
更新日期:2015-02-01 00:00:00
abstract:PURPOSE:Phosphomono- and diesters, the major components of the choline peak in (1) H magnetic resonance spectroscopy, are associated with membrane anabolic and catabolic mechanisms. With the refocused insensitive nuclei-enhanced polarization transfer technique, these phospholipids are edited and enhanced in the (31) P ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.25278
更新日期:2015-04-01 00:00:00
abstract::1H transverse nuclear magnetic relaxation times (T2) and self-diffusion coefficients (SDCs) of water were measured in isolated proteoglycan aggregates from pig articular cartilage. The influence of varying osmotic pressure, as well as of different calcium concentrations, on the samples was investigated. Due to a struc...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/(sici)1522-2594(199901)41:1<43::aid-mrm8>3
更新日期:1999-01-01 00:00:00
abstract::The mechanics of the macrocirculation are examined as a basis for imaging blood flow within the system. The macrocirculation is defined as that part of the circulation in which inertial forces are not negligible compared to viscous forces and as such in human subjects comprises the heart and large blood vessels. Becau...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910140204
更新日期:1990-05-01 00:00:00
abstract::The dependence of 1/T1 on the magnetic field strength (the relaxation dispersion) has been measured at 37 degrees C on autopsy samples of human brain gray and white matter at field strengths corresponding to proton Larmor frequencies between 10 kHz and 50 MHz (0.0002-1.2 T). Additional measurements of 1/T1 and 1/T2 ha...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910160212
更新日期:1990-11-01 00:00:00
abstract::MRI of myocardial perfusion observed with an extracellular contrast agent has proven valuable for the detection of coronary artery disease. During contrast enhancement transient dark rim artifacts are sometimes visible, complicating diagnosis and quantification. In this work a quantification of the frequency offsets c...
journal_title:Magnetic resonance in medicine
pub_type: 临床试验,杂志文章
doi:10.1002/mrm.21723
更新日期:2008-10-01 00:00:00
abstract:PURPOSE:Sensitivity encoding (SENSE) reconstruction of multiband echo planar imaging (EPI) may cause artifacts when simultaneously excited slices require different phase correction to remove the EPI-specific ghost shifted by half of the matrix size (N). We propose a simplified solution of this problem that combines SEN...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.25915
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:To develop an accelerated, robust, and accurate diffusion MRI acquisition and reconstruction technique for submillimeter whole human brain in vivo scan on a clinical scanner. METHODS:We extend the ultra-high resolution diffusion MRI acquisition technique, gSlider, by allowing undersampling in q-space and radio...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.28232
更新日期:2020-10-01 00:00:00
abstract::Excitation by conventional B1 pulses in surface coil chemical-shift imaging experiments yields resonance intensities that vary greatly with position. B1-compensated semi-selective pulses overcome much of this problem. These pulses are employed for excitation and refocusing in 1H NMR spectroscopic localization in the i...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章
doi:10.1002/mrm.1910250118
更新日期:1992-05-01 00:00:00
abstract::Adipose tissue as well as other depots of fat (triglycerides) are increasingly being recognized as active contributors to the human function and metabolism. In addition to the fat concentration, also the fatty acid chemical composition (FAC) of the triglyceride molecules may play an important part in diseases such as ...
journal_title:Magnetic resonance in medicine
pub_type: 杂志文章,评审
doi:10.1002/mrm.28471
更新日期:2021-01-01 00:00:00