Comparison of methodologies for detecting Trypanosoma cruzi parasites by microscopic observation of microhematocrit capillary tubes.

Abstract:

INTRODUCTION:The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood. METHODS:The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity. RESULTS:The rotating method was easier, faster, and more sensitive than the others with 100% specificity. CONCLUSIONS:The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.

journal_name

Rev Soc Bras Med Trop

authors

Vera-Ku M,Meza-González G,Carlier Y,Truyens C,Gamboa-León R

doi

10.1590/0037-8682-0505-2018

subject

Has Abstract

pub_date

2019-06-27 00:00:00

pages

e20180505

eissn

0037-8682

issn

1678-9849

pii

S0037-86822019000100671

journal_volume

52

pub_type

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