Abstract:
:When the human T cell lymphotropic virus (HTLV) is integrated with the host cell genome (provirus), its proviral DNA is a replication marker. Proviral load appears to be an important factor in the development of diseases related to these retroviruses. In this study, a methodology for absolute quantification of the HTLV-1 and HTLV-2 proviral load using real-time PCR was developed. Fifty-three blood donor samples with positive ELISA test result were subjected to this methodology, which utilized the TaqMan system for three target sequences: HTLV-1, HTLV-2 and albumin. The absolute proviral load was quantified using the relative ratio between the HTLV genome and the host cell genome, taking into consideration the white blood cell count. The method presented is sensitive (215 copies/ml), practical and simple for proviral quantification, and is efficient and appropriate for confirming and discriminating infections according to viral type.
journal_name
Rev Soc Bras Med Tropjournal_title
Revista da Sociedade Brasileira de Medicina Tropicalauthors
Tamegão-Lopes BP,Rezende PR,Maradei-Pereira LM,de Lemos JAdoi
10.1590/s0037-86822006000600007subject
Has Abstractpub_date
2006-11-01 00:00:00pages
548-52issue
6eissn
0037-8682issn
1678-9849pii
S0037-86822006000600007journal_volume
39pub_type
杂志文章abstract::We report a case of intestinal involvement of Paracoccidioidomycosis, in a patient considered to have colonic cancer. The diagnosis of this mycosis should be considered when an abdominal mass associated with intra-lesional calcifications on X-ray is observed. CT scans increase the findings. ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章,评审
doi:10.1590/s0037-86822000000300010
更新日期:2000-05-01 00:00:00
abstract:INTRODUCTION:Hepatitis B is common in Brazil, although there are regional differences regarding the degree of endemicity, the most frequent forms of transmission and the presence of different evolutive stages of chronic disease. The present study aimed to determine the clinical, demographic and epidemiological characte...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822011000100004
更新日期:2011-01-01 00:00:00
abstract::The prevalence of antibodies against Yersinia pestis in domestic carnivores (dogs and cats), in plague areas in the State of Ceará, was analyzed to establish the importance of monitoring these animals within the routine practice of the plague control program. Over the decade 1997-2006, 146,732 serum samples were exami...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822009000600019
更新日期:2009-11-01 00:00:00
abstract::We present a case of human intoxication due to a snakebite by the opisthoglyphous dipsadid Thamnodynastes lanei. A 26-year-old man was bitten on the right hand and was not medicated. Bleeding lasted a few seconds, while paresthesia, chills, and headache persisted for up to 10 hours. The pain disappeared after a week, ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type:
doi:10.1590/0037-8682-0194-2020
更新日期:2020-11-13 00:00:00
abstract:INTRODUCTION:The increase in American tegumentary leishmaniasis (ATL) cases in 2005 was due to an outbreak that occurred in the towns of Itapema and Balneário Camboriú. This study aimed to determine the possible presence of infection by Leishmania sp in domestic dogs living in an endemic focus of ATL in the town of Bal...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822010000600023
更新日期:2010-11-01 00:00:00
abstract::The first choice of treatment for American cutaneous leishmaniasis is the pentavalent antimonial drug. Although it has been shown that this treatment is mostly effective and indicated, some disadvantages should be taken into account such as side effects, long term treatment inconveniences and counter-indication for pa...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822006000100003
更新日期:2006-01-01 00:00:00
abstract:INTRODUCTION: :This study analyzed blackfly awareness and perceptions of health education practices for blackfly control among vulnerable populations in Brazil. METHODS: :An exploratory descriptive quantitative research analysis was performed to investigate and analyze the awareness of and potential participation in b...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0264-2016
更新日期:2017-05-01 00:00:00
abstract::Present report demonstrates that repeated radiation of Schistosoma mansoni-infected Biomphalaria glabrata, totaling 15,000 rads, caused a sudden, albeit transient, suppression of cercarial shedding. Initially, sporocysts practically disappeared from the snail tissues. The more resistant developing cercariae presented ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822004000300005
更新日期:2004-05-01 00:00:00
abstract:INTRODUCTION:Rabies is an acute zoonotic disease, caused by a rhabdovirus that can affect all mammals, and is commonly transmitted by the bite of a rabid animal. The definitive diagnosis is laboratorial, by the Fluorescent Antibody Test (FAT) as a quick test and Mouse Inoculation Test (MIT) as a confirmatory test (gold...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0303-2017
更新日期:2018-01-01 00:00:00
abstract:INTRODUCTION:This study aimed to analyze social factors involved in the spatial distribution and under-reporting of tuberculosis (TB) in the city of Vitória, Espírito Santo State, Brazil. METHODS:This was an ecological study of the reported cases of TB between 2009 and 2011, according to census tracts. The outcome was...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0015-2018
更新日期:2018-09-01 00:00:00
abstract::Some epidemiological characteristics of head lice, Pediculus capitis, were studied using two procedures: cut hair analysis and head inspection. Higher prevalence rates were observed in the middle and at the end of the school terms. Both procedures indicated that children were the main reservoir for this type of pedicu...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822007000200022
更新日期:2007-03-01 00:00:00
abstract::Vectorial, transfusion and congenital are considered the main transmission mechanisms in human Chagas disease. Alternative mechanisms are accidental, oral and by organ transplantation. Other hypothetic mechanisms could be by other vectors, sexual, criminal and by means of marsupial anal secretions. The present accorde...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822011000800011
更新日期:2011-01-01 00:00:00
abstract::Two cases of malaria by Plasmodium vivax relapsed after treatment with drugs in doses recommended by the Ministry of Health are presented. Both patients were overweight and were followed in the Federal District, an area considered free from vector transmission of the disease. Radical cure was obtained after medication...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822010000600033
更新日期:2010-11-01 00:00:00
abstract::In order to estimate the incidence of and risk factors for developing tuberculosis, the clinical charts of a retrospective cohort of 281 HIV-positive adults, who were notified to the AIDS Program of the Health Department of Brasilia in 1998, were reviewed in 2003. All the patients were treatment-naive regarding antire...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章,多中心研究
doi:10.1590/s0037-86822008000600001
更新日期:2008-11-01 00:00:00
abstract:INTRODUCTION:There was a follow-up of pregnant women treated for multidrug-resistance tuberculosis (MDR-TB) during pregnancy in Campinas, State of São Paulo, Brazil, from 1995 to 2007. METHODS:In a retrospective study, patients with tuberculosis who were resistant to at least isoniazid and rifampicin and had pregnancy...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
abstract::The inflammatory processes that develop during the advanced stages of hepatic schistosomiasis mansoni have been related in this study to: (a) accumulation of siderosomes; (b) capacity of the ferrous/ferric ions to unleash the formation of free radicals; (c) peroxidation of membrane lipids and; (d) reduction of stabili...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86821990000200003
更新日期:1990-04-01 00:00:00
abstract:INTRODUCTION:With the globalization of Chagas disease, unexperienced health care providers may have difficulties in identifying which patients should be examined for this condition. This study aimed to develop and validate a diagnostic clinical prediction model for chronic Chagas disease. METHODS:This diagnostic cohor...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0196-2016
更新日期:2016-05-01 00:00:00
abstract::The presence of rickettsial infection was surveyed in 3,545 Amblyomma cajennense ticks and 2,666 Amblyomma dubitatum ticks. Using the hemolymph test, polymerase chain reaction and isolation of Rickettsia in cell cultures, all of the Amblyomma cajennense were negative, whereas 634 (23.8%) of the Amblyomma dubitatum tic...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822009000300023
更新日期:2009-05-01 00:00:00
abstract:INTRODUCTION:Chagas disease is caused by Trypanosoma cruzi. Wild and perianthropic mammals maintain the infection/transmission cycle, both in their natural habitat and in the peridomestic area. The aim of this paper was to present the results from a study on wild rodents in the central and northern regions of San Luis ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822010000300007
更新日期:2010-05-01 00:00:00
abstract::The increasing number of HIV/AIDS infected women is one of the main characteristics of the AIDS epidemic, besides the wide expansion of the disease among the poor. This study focused on a group of 82 women with AIDS from Salvador-Bahia. The mean age was 32 +/- 7.2 years, 75% had children, 82.5% were jobless, and 77.8%...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822004000600002
更新日期:2004-11-01 00:00:00
abstract::In vivo antimalarial drug efficacy studies of uncomplicated Plasmodium falciparum malaria at an isolated site in the Amazon basin of Peru bordering Brazil and Colombia showed >50% RII/RIII resistance to sulfadoxine-pyrimethamine but no evidence of resistance to mefloquine. ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822004000300015
更新日期:2004-05-01 00:00:00
abstract::Parasite resistance can be defined as the ability of a parasite strain to survive and/or to multiply despite the administration and absorption of a drug given in doses equal or higher than those usually recommended, but within the limits of tolerance of the patients. Therefore, the ideal study design to be used to mon...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/s0037-86822003000300011
更新日期:2003-05-01 00:00:00
abstract:INTRODUCTION:We compared feces culturing in charcoal or vermiculite to obtain Strongyloides venezuelensis larvae. METHODS:Feces (5 g) from infected rats was mixed with vermiculite (10 g) or coal (10 g) in plastic cups and incubated at 28°C for 48 h. Larvae were recovered using Baermann-Moraes method. RESULTS:Signific...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0019-2014
更新日期:2014-09-01 00:00:00
abstract:INTRODUCTION:This study aimed to describe the epidemiology of congenital and maternal syphilis in the Brazilian Federal District in 2010. METHODS:A retrospective descriptive study was conducted on the basis of the cases recorded in the System of Notifiable Disease Information. RESULTS:The study population compri...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0168-2014
更新日期:2015-03-01 00:00:00
abstract:INTRODUCTION:This paper presents the results of an extensive survey of freshwater mollusks in the Simplício Queda Única Hydroelectric Development area, Southeast Brazil. METHODS:Mollusks were collected between 2008 and 2013. All specimens were examined for the presence of larval trematodes. RESULTS:In total, 12,507 s...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0334-2017
更新日期:2018-09-01 00:00:00
abstract:INTRODUCTION::We evaluated the associations between interleukin-10 (IL-10) gene polymorphisms -G1082A/-C819T/-C592A and periportal fibrosis regression after specific treatment for schistosomiasis. METHODS::This retrospective cohort study involved 125 Brazilian patients infected with Schistosomiasis mansoni, who were f...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0141-2016
更新日期:2016-11-01 00:00:00
abstract::In order to study certain epidemiological features of multidrug-resistant tuberculosis (MDR-TB) carriers and their influence on the control and treatment, a group of patients was evaluated over a four-year period, selected by: Mycobacterium tuberculosis isolation from sputum; resistance to Rifampin, Isoniazid and one ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:INTRODUCTION:Coccidioidomycosis, a disease caused by Coccidioides immitis or Coccidioides posadasii, is endemic in arid climatic regions in Northeast Brazil. Its prevalence is higher among young adult males living in rural areas. Existing literature about this disease in Ceará, a Northeast Brazilian state, are scarce. ...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0249-2020
更新日期:2020-10-21 00:00:00
abstract:INTRODUCTION:This study confirmed the absence of natural infection with Xenotropic murine leukemia virus-related virus (XMRV) or XMRV-related disease in human populations of the Brazilian Amazon basin. We demonstrated that 803 individuals of both sexes, who were residents of Belem in the Brazilian State of Pará, were n...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0075-2014
更新日期:2014-05-01 00:00:00
abstract:INTRODUCTION:This study evaluates the factors associated with the development of severe periportal fibrosis in patients with Schistosoma mansoni. METHODS:A cross-sectional study was conducted from April to December 2012 involving 178 patients infected with S. mansoni who were treated in the Hospital das Clínicas of Pe...
journal_title:Revista da Sociedade Brasileira de Medicina Tropical
pub_type: 杂志文章
doi:10.1590/0037-8682-0110-2013
更新日期:2013-07-01 00:00:00