Abstract：
:A regulatory T (Treg) cell/T helper 17 (Th17) cell imbalance is involved in many autoimmune diseases. Rapamycin (Rapa), a clinically used immunosuppressive drug, has been shown to inhibit Th17 cell differentiation but promote Treg cell generation. In this study, we aimed to study the mechanism of Rapa acting on Treg and Th17 cell differentiation. Purified mouse CD4+CD25- T cells were stimulated and polarized in vitro to generate Th17 or Treg cells in the presence or absence of Rapa. We first confirmed that Rapa inhibited the differentiation of Th17 cells and greatly promoted Treg cell generation in vitro. As metabolic pathways play a key role in T cell differentiation, we then detected the metabolic programs in Rapa-treated T cells. We found that Rapa blocked glycolysis in induced Th17 cells, evidenced by reduced glucose uptake, and inhibited expression of glucose transporter 1 and the rate-limiting enzyme HK2. In addition, the expression of c-Myc and of HIF-1α transcription factor, which regulate many genes involved in glycolysis, were inhibited by Rapa. Conversely, Rapa promoted fatty acid oxidation (FAO) metabolism in differentiated Treg cells, with the elevation of FAO product β-hydroxybutyrate, and increased expression of ATGL and CPT1A, the key enzymes of FAO in differentiated Treg cells. The expression of phospho-AMPKα, the key signal in the regulation of FAO, was also promoted in Rapa-treated induced Treg cells. Together, these findings indicated that Rapa abrogated glycolysis in Th17 cells but facilitated FAO in induced Treg cells, which may underlie the mechanism by which Rapa regulates the Treg/Th17 balance.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Zhang J,Jin H,Xu Y,Shan Jdoi
10.1016/j.transproceed.2019.04.067subject
Has Abstractpub_date
2019-01-01 00:00:00pages
2136-2140issue
6eissn
0041-1345issn
1873-2623pii
S0041-1345(18)31633-6journal_volume
51pub_type
杂志文章abstract：:Pancreatic islets have been the focus of recent studies exploring the pathologic mechanisms of diabetes mellitus as well as more effective and radical treatments for this disease. Islet transplantation is a promising therapeutic strategy; however, isolation of pancreatic islets for this purpose has been challenging, b...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2013.11.074
更新日期：2014-05-01 00:00:00
abstract：:Calcineurin inhibitor (CNI) immunosuppressive therapy post-liver transplantation (OLT) is important to reduce graft rejection episodes. However, these drugs show important side effects, particularly renal dysfunction (RDF). Changing from CNI to a nonnephrotoxic drug, as mammalian target of rapamycin (mTOR) inhibitor m...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.06.009
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND:Hepatitis C viral graft reinfection is almost a universal event after liver transplantation with consequent disease progression. METHODS:We applied triple therapy (n = 21) with the use of telaprevir (TVR; n = 12) or simeprevir (SVR; n = 9). RESULTS:TVR was given at the dose 1,500 mg daily (n = 11) with red...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.10.058
更新日期：2015-04-01 00:00:00
abstract：:Fulminant hepatic failure (FHF) is often complicated with cerebral edema, intracranial hypertension, and coma. Cytotoxic and vasogenic factors have been recognized in the etiology of cerebral edema. One of the main causes seems to be the accumulation of glutamine in astrocytes, which is produced from ammonia and the e...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.01.040
更新日期：2006-04-01 00:00:00
abstract：INTRODUCTION:In rural areas it is common to find unfavorable attitudes toward organ donation, and therefore it is important to find out the attitude and profile of new generations for improving predisposition to organ donation in these areas. Our objective was to analyze the attitude toward organ donation and the relat...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.09.054
更新日期：2018-03-01 00:00:00
abstract：:Acute liver failure (ALF) counts for 9%-11% of activity in leading liver transplant programs. We have summarized the Hungarian Liver Transplant Program experience for ALF among 412 consecutive orthotopic liver transplantations (OLTs). All OLTs were performed without an extended international donor background. The prop...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.03.076
更新日期：2011-05-01 00:00:00
abstract：:Understanding the attitudes and willingness in regard to living organ donation of health care professionals is important for clinical practice and needs to be addressed. Thus, the objective of this study was to examine willingness and its influencing factors in regard to living organ donation in clinical health care p...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.02.179
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:Endothelial dysfunction (ED) is a common, early abnormality that predisposes patients to develop atherosclerosis and cardiovascular events; inflammation is associated with atherosclerosis and malnutrition. Patients with failed transplants are usually complicated by inflammation; however, ED in this group of ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.06.200
更新日期：2009-11-01 00:00:00
abstract：INTRODUCTION:Hepatitis C Viral (HCV) infection is the leading cause of chronic liver disease in end-stage renal disease patients (ESRD). The impact of HCV on patient and graft survival posttransplantation is controversial. The most successful approach is to eliminate the virus while the patient is on dialysis prior to ...
journal_title：Transplantation proceedings
pub_type： 临床试验,杂志文章
doi：10.1016/j.transproceed.2004.07.025
更新日期：2004-07-01 00:00:00
abstract：:This retrospective review presented the prevalence and manifestations of tuberculosis among renal transplant recipients in our center between 1987 and mid 2007. The prevalence of tuberculosis was 5/151 (3.3%) recipients with a median age of 49 years (range = 38-55). The median time of diagnosis after transplantation w...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.06.020
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND:In paired living kidney exchange donation from an old donor to a young recipient, it may be argued that elderly donors provide an inferior quality kidney. However, the impact of donors older than recipients on transplant outcomes remains unclear. METHODS:We retrospectively reviewed the charts of primary liv...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.12.013
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Hyperparathyroidism is common in end-stage renal disease. It often persists following renal transplantation (RTx) and remains elusive to manage due to the lack of evidence base. We therefore present observational data describing the natural history and management of hypercalcemia and hyperparathyroidism foll...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2016.09.050
更新日期：2016-12-01 00:00:00
abstract：:Solid organ transplant recipients undergoing immunosuppressive therapy are considered to be at high risk for serious infectious complications. Recently in the United States, a pandemic of H1N1 flu infection has been reported with serious complications. We describe H1N1 infection in a living kidney donor and the 42-yea...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.09.052
更新日期：2009-12-01 00:00:00
abstract：BACKGROUND:Because liver allograft steatosis is an important risk factor of graft dysfunction after liver transplantation, it must be taken into consideration during graft acceptance. The aim of this study was to evaluate the reliability of frozen section in the assessment of liver steatosis before transplantation. ME...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.09.102
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND:Human herpes virus (HHV8) is associated with Castleman's disease, primary effusion lymphoma, and the Kaposi's sarcoma (KS). PATIENTS AND METHODS:Among 3815 solid organ transplants performed at our center between 1977 and 2003, five patients (0.1%) were identified with KS. RESULTS:There were one cardiac, on...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.03.144
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND:Hepatic ischemia-reperfusion injury (IRI) is an important determinant of the outcome of hepatic surgery, including re-section and transplantation. Previous studies have shown that nitric oxide (NO) has a protective effect against IRI. Therefore, many studies have examined methods for supplying NO. In this st...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.03.054
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND:This study was undertaken to investigate risk factors of mortality in patients with fulminant hepatic failure (FHF). METHODS:Fifty-three patients with FHF treated from January 2006 to April 2011 were allocated to a spontaneous survival group (group 1), a death without liver transplantation (LT) group (group...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2013.08.036
更新日期：2013-10-01 00:00:00
abstract：INTRODUCTION:Real-time contrast-enhanced sonography (CES) can assess microvascular tissue perfusion using gas-filled microbubbles. The purpose of the study was to evaluate the feasibility of early CES in predicting long-term kidney allograft function in comparison to color Doppler ultrasonography (CDUS). METHODS:We pr...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.04.013
更新日期：2014-12-01 00:00:00
abstract：:A new model of tissue-engineered artificial autologous human skin developed in Andalusia is currently being transplanted into patients suffering from large burns within the Andalusian Public Healthcare System. This product is considered an advanced therapy medicinal product (ATMP) in Europe, and its clinical use impli...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.08.014
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:The safety of the living donor in living-donor liver transplantation (LDLT) is always the first priority, meanwhile, the graft-to-recipient weight ratio (GRWR) and the anatomy of the liver allograft must also not be compromised in order to warrant tranplatation success. When it comes to the allograft of the ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.04.050
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND:The first New Delhi metallo-beta-lactamase (NDM)-producing bacteria were isolated in 2008 in the world, and in 2011 in Poland. Due to the high clonal diversity (17 types) of their blaNDM gene, encoded on (Tn125-like) mobile genetic elements, these strains usually exhibit resistance to nearly all available an...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.04.002
更新日期：2018-09-01 00:00:00
abstract：PURPOSE:We aim to see the rate of progression to chronic kidney disease stage III after living donor nephrectomy in a single institution annually. METHODS:Between May 2006 and July 2017, a total of 753 living kidney donors who were followed up more than 6 months were enrolled in the study. We divided normal function v...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.03.058
更新日期：2019-10-01 00:00:00
abstract：INTRODUCTION:Daclizumab is a monoclonal antibody that binds to the interleukin-2 receptor. It has been used as induction therapy in heart transplantation with two to five repeated administrations over several weeks. The objective of our study was to estimate the efficacy and safety of induction therapy with only one do...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.10.086
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:Southeastern Spain is a habitual area of residence for the native population of Scotland. OBJECTIVE:To assess the attitude toward donation in the immigrant population in our regional community who were born in Scotland and to analyze the variables that influence this attitude. MATERIALS AND METHODS:A rando...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.08.099
更新日期：2008-11-01 00:00:00
abstract：:Postoperative biliary tract complications after liver transplantation (LT) still lead to early and late morbidity and mortality. Modern interventional endoscopic techniques can replace surgical repair as the first line of treatment. Nevertheless surgical intervention plays an important role in specific situations. We ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.09.045
更新日期：2010-12-01 00:00:00
abstract：INTRODUCTION:An increased risk of skin cancer is particularly important in patients undergoing maintenance hemodialysis (HD), who are potential transplant recipients. In transplant recipients who are exposed to immunosuppressive therapy, neoplastic skin disease my take a more aggressive course. Increased exposure to ph...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.02.098
更新日期：2018-01-01 00:00:00
abstract：INTRODUCTION:Because of the shortage of organs available for transplantation, living related sequential transplantation with the use of liver and a kidney from the same donor has emerged as a reasonable therapeutic alternative. However, there is insufficient literature about the complications that living donors experie...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2017.01.020
更新日期：2017-04-01 00:00:00
abstract：INTRODUCTION:Nowadays, lung transplantation (LTx) allocation in Brazil is based mainly on waiting time. There is a need to evaluate the equity of the current lung allocation system. OBJECTIVES:We sought to (1) determine the characteristics of registered patients on the waiting list and (2) identify predictors of death...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.01.048
更新日期：2009-04-01 00:00:00
abstract：:Acute dysfunction of cardiac allograft without evidence of cellular rejection is a potentially fatal complication of heart transplantation that suggests a humoral origin. In clinical practice, humoral rejection (HR) is suspected when there is evidence of severe allograft dysfunction but endomyocardial biopsy (EMB) sho...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.03.033
更新日期：2004-04-01 00:00:00
abstract：BACKGROUND AND AIMS:Frailty is associated with increased morbidity and mortality, and this is tightly linked to liver decompensation and increased complication rates among liver transplant (LT) candidates. The aim of the study was to evaluate the efficacy of a structured in- and outpatient exercise training program for...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.08.045
更新日期：2019-12-01 00:00:00
