Abstract：
:Pancreatic islets have been the focus of recent studies exploring the pathologic mechanisms of diabetes mellitus as well as more effective and radical treatments for this disease. Islet transplantation is a promising therapeutic strategy; however, isolation of pancreatic islets for this purpose has been challenging, because the technique is time consuming and technically difficult, and tissue handling can be variable. Pseudo-islets can be used as an alternative to naïve islets, but require cellular sources or artificial materials. In this study, pancreas-derived cells were used to generate pseudo-islets. Because the pancreas is composed of a variety of cell types, namely α cells, β cells, δ cells, and other pancreatic cells that perform different functions, we used 3 different cell lines-NIT-1 (a β-cell line), α TC1 clone 6 (an α-cell line), and TGP52 (a pancreatic epithelial-like cell line)-which we cocultured in nonadhesive culture plates to produce hybrid cellular spheroids. These pseudo-islets had an oval shape and were morphologically similar to naïve islets; additionally, they expressed and secreted the pancreatic hormones insulin, glucagon, and somatostatin, as confirmed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay. The results demonstrate that pseudo-islets that mimic naïve islets can be successfully generated by a coculture method. These artificial islets can potentially be used for in vitro tests related to diabetes mellitus, specifically, in drug discovery or for investigating pathology. Moreover, they can be useful for examining basic questions pertaining to cell-cell interactions and tissue development.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Jo YH,Jang IJ,Nemeno JG,Lee S,Kim BY,Nam BM,Yang W,Lee KM,Kim H,Takebe T,Kim YS,Lee JIdoi
10.1016/j.transproceed.2013.11.074subject
Has Abstractpub_date
2014-05-01 00:00:00pages
1156-60issue
4eissn
0041-1345issn
1873-2623pii
S0041-1345(13)01288-8journal_volume
46pub_type
杂志文章abstract：:Pseudoaneurysm is a rare vascular complication in pancreas transplantation that can lead into life-threatening situations. A 44-year-old male patient after simultaneous pancreas-kidney transplant was admitted to the hospital for routine tests 3 months after surgery. A computed tomography scan and ultrasound examinatio...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2020.02.108
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVES:Donors with bacteremia and sepsis are often considered to be controversial for organ retrieval due to potential transmission of an infectious agent to the recipient. Herein we report our initial experience of organ donation and transplantation results from donors with systemic infection. MATERIALS AND METHO...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2016.02.092
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND:The presence of antiphospholipid antibodies (APLAs) may be associated with increased thrombotic risk. Liver graft thrombosis may necessitate retransplantation. AIM:To determine the prevalence of APLAs among liver recipients and to investigate the relationship between APLAs and liver graft thrombosis. MATER...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2013.01.024
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to describe the clinical and microbiological characteristics of the infectious complications among simultaneous pancreas-kidney transplantations (SPKT). MATERIALS AND METHODS:Among the first 45 SPKT the mean age was 34 years (range, 21 to 49) and the mean duration of follow-up 1...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.03.114
更新日期：2004-05-01 00:00:00
abstract：UNLABELLED:A primary reason to eliminate corticosteroids from immunosuppressive regimens in solid organ transplant recipients is improved cardiovascular risk profiles. Although a number of studies have documented that corticosteroid withdrawal (CSWD) regimens reduce hypertension, hyperlipidemia, diabetes, and weight ga...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.01.060
更新日期：2005-03-01 00:00:00
abstract：:Cytomegalovirus (CMV) seronegative renal allograft recipients (R-), particularly those with a graft from a CMV-seropositive donor (D+), are at high risk for primary CMV infection. CMV resistance to antiviral oral therapy is an emerging problem in renal transplantation, prompting development of new prophylactic strateg...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.07.001
更新日期：2006-09-01 00:00:00
abstract：:Epithelioid hemangioendothelioma (EHE) is a rare neoplasm of vascular origin, but unknown etiology that occurs in the liver, lungs and other organs. Its hepatic form (HEHE) generally behaves as a low-grade malignant tumor with a slowly progressive phenotype. Surgical resection or liver transplantation (OLT) has been r...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.12.004
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVES:The complement-dependent lymphocytotoxicity crossmatch (CDC-XM) detects cytotoxic parameters of preformed antibodies. The flow cytometric crossmatch (FCXM) is used to detect the binding of recipient antibodies to donor cells. Because these two assays provide different information, both methods are often perf...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.11.024
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND:Cardiovascular mortality rate in patients with end-stage renal disease is 3 magnitudes higher than in the general population; it remains 10-fold higher after successful renal transplantation (Tx). Among others, obesity and hypertension can exert deleterious effects on vascular structure and function after Tx...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.05.060
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:Conversion from calcineurin inhibitors (CNIs) to mammalian target-of-rapamycin inhibitors (mTORi) was systematically evaluated in heart transplant recipients (HTRs) for the first time. METHODS:MEDLINE (PUBMED), EMBASE, Cochrane Library, and clinical trial registries were searched comprehensively. After scree...
journal_title：Transplantation proceedings
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.transproceed.2015.09.059
更新日期：2015-12-01 00:00:00
abstract：:We have studied both specific and non-specific suppression in allogeneic combinations. On the one hand, achievement of specific suppression may circumvent both HVGR and GVHR, while on the other, Cy-resistant suppressor cells may exacerbate the GVHR in bone marrow graft recipients. A greater understanding of both of th...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：INTRODUCTION:In this study, hemoglobin (Hb) concentrations secondary to enalapril (E) or losartan (L) therapy were evaluated with respect to renin-angiotensin system (RAS) polymorphisms in renal transplant recipients. MATERIALS AND METHODS:After determination of RAS polymorphisms [angiotensin-converting enzyme (DD, no...
journal_title：Transplantation proceedings
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.transproceed.2007.02.016
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:The reliability of endoscopic findings after adult intestinal transplantation on short-term follow-up has been shown. The aim of this study was to evaluate in a long-term follow-up the diagnostic value of endoscopies compared with the biopsy value. METHODS:We evaluated 52 endoscopies over a period of 2 year...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.07.025
更新日期：2014-09-01 00:00:00
abstract：:Conceptually, pancreas islet transplantation (PIT) associated with renal transplantation (RT) should resolve not only chronic renal failure but also diabetes. Although the most frequently used site for PIT is the portal vein, genitourinary locations could be technically feasible during RT. Seventeen pigs (age 3 to 4 m...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.08.068
更新日期：2006-10-01 00:00:00
abstract：INTRODUCTION:We aimed to evaluate if ex vivo machine perfusion could minimize the negative impact of cold ischemia on those renal grafts obtained from controlled donation after circulatory death (cDCD). MATERIAL AND METHODS:Prospective observational paired study of kidney transplants from cDCD performed in our center....
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.12.010
更新日期：2019-03-01 00:00:00
abstract：:Mitral valve dysfunction after orthotopic heart transplantation may cause symptoms refractory to medical therapy. In this report, we present a patient who underwent mitral annuloplasty for severe symptomatic mitral valve insufficiency 9 years after heart transplantation, and we critically appraise the literature avail...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.10.010
更新日期：2008-06-01 00:00:00
abstract：:We present the case of a 37-year-old patient diagnosed with multiple endocrine neoplasia type 2A (MEN 2A) syndrome, as confirmed by genetic tests, who underwent the transplantation of a kidney from a cadaveric donor. MEN 2A, a hereditary autosomal dominant syndrome, is caused by the mutation of the RET proto-oncogene....
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.08.018
更新日期：2011-10-01 00:00:00
abstract：:The gold standard to assess renal function is the measurement of glomerular filtration rate (GFR). For practical reasons, renal function is often evaluated from serum creatinine (S Cr) or cystatin C (S Cys), and GFR is predicted from SCr. Ultrasound scanning of the kidneys is used only to evaluate renal morphology. Th...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2007.05.003
更新日期：2007-07-01 00:00:00
abstract：AIM:To determine a formula predicting the standard liver volume based on body surface area (BSA) or body weight in Chinese adults. MATERIALS AND METHODS:A total of 115 consecutive right-lobe living donors not including the middle hepatic vein underwent right hemi-hepatectomy. No organs were used from prisoners, and no...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.08.079
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:Usually an experimental necrotizing enterocolitis experimental model, we Investigated nitric oxide levels in intestinal tissues of newborn mice with or without l-arginine therapy during sessions of ischemia and reoxygenation. METHODS:Twenty-six newborn mice from the Wistar EPM-1 lineage, weighing from 4.5 to...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.02.044
更新日期：2008-04-01 00:00:00
abstract：:Specialized antigen-presenting cells (APC), known as dendritic cells (DC), play a pivotal role in initiating primary immune responses. Several vector systems, including adenoviral vectors, retroviral vectors, hemagglutinating virus of Japan-related vectors, and the electroporation, have been shown to transduce genes i...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.12.234
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND:Acute liver failure (ALF) leads to high morbidity and mortality and is characterized by an accelerated deterioration of hepatic function in patients without prior liver disease. The survival rate is <15% without liver transplantation (LT). The aim of this study was to describe the population of patients with...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2018.01.011
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:Oxidative stress is one of the most important mechanisms to explain genesis of the complications in the chronic progression of diabetes. In this investigation we studied the effects of pancreas transplantation (PT) on the imbalance caused by excessive production of free oxygen radicals by antioxidant defenses o...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.01.050
更新日期：2008-03-01 00:00:00
abstract：:The aim of this study was to evaluate the impact of sirolimus and cyclosporine (CsA) combined therapy on the incidence and duration of delayed graft function (DGF), and the impact of the latter on 1-year graft function. The study entailed 23 cadaveric renal recipients treated with sirolimus-CsA-prednisone regimen (sir...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2004.12.010
更新日期：2005-03-01 00:00:00
abstract：INTRODUCTION:We sought to evaluate the efficacy of enteric-coated mycophenolate sodium (EC-MPS) and the gastrointestinal (GI) adverse events in de novo kidney transplant recipients. METHODS:This noncontrolled, retrospective review includes 22 de novo kidney transplant recipients. All patients received a standard cours...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.03.105
更新日期：2005-06-01 00:00:00
abstract：BACKGROUND:Islet allograft rejection in sensitized recipients is difficult to control by costimulation blockade using anti-CD154 and cytotoxic T-lymphocyte antigen-4 immunoglobulin (CTLA4Ig). Because leukocyte function antigen (LFA) 1 is highly expressed on memory T cells, adding an LFA-1 blockade may inhibit memory T-...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.05.084
更新日期：2010-07-01 00:00:00
abstract：INTRODUCTION:The administration of interlukin-2 receptor antagonists for induction therapy has reduced the incidence of acute rejection episodes in kidney transplantation. Although some studies have investigated the efficacy of these monoclonal antibodies, there is little experience among Iranian kidney transplant reci...
journal_title：Transplantation proceedings
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.transproceed.2009.06.180
更新日期：2009-09-01 00:00:00
abstract：BACKGROUND:Living related liver transplantation (LRLT) for hepatocellular carcinoma (HCC) in cirrhotic patients has emerged as a rewarding therapy for a cure. Extensions of the Milan criteria have been proposed with encouraging results. PATIENTS AND METHODS:From October 2001 to June 2004, 47 adult patients with end-st...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.08.037
更新日期：2005-09-01 00:00:00
abstract：:Antibody-mediated rejection (AMR) has been recognized recently as an important cause of graft failure. Detection of C4d in renal allograft biopsies is a proven ancillary technique in the diagnosis of AMR. The prevalence of C4d staining in Western countries varies from 17% to 60% among indication biopsies. There are on...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.04.015
更新日期：2009-11-01 00:00:00
abstract：:Significantly lower graft survival has been observed among recipients of a third (G3) compared with a first or second kidney transplantation. Because patients awaiting G3 are largely HLA immunized, they are usually transplanted with a high HLA match. Moreover, their rate of acute rejection episodes is similar to a fir...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.12.007
更新日期：2009-03-01 00:00:00
