Abstract:
:Ulvan lyases can degrade ulvan to oligosaccharides with potent biological activity. A new ulvan lyase gene, ALT3695, was identified in Alteromonas sp. A321. Soluble expression of ALT3695 was achieved in Escherichia coli BL21 (DE3). The 1314-bp gene encoded a protein with 437 amino acid residues. The amino acid sequence of ALT3695 exhibited low sequence identity with polysaccharide lyase family 25 (PL25) ulvan lyases from Pseudoalteromonas sp. PLSV (64.14% identity), Alteromonas sp. LOR (62.68% identity), and Nonlabens ulvanivorans PLR (57.37% identity). Recombinant ALT3695 was purified and the apparent molecular weight was about 53 kDa, which is different from that of other polysaccharide-degrading enzymes identified in Alteromonas sp. A321. ALT3695 exhibited maximal activity in 50 mM Tris-HCl buffer at pH 8.0 and 50 °C. ALT3695 was relatively thermostable, as 90% activity was observed after incubation at 40 °C for 3 h. The Km and Vmax values of ALT3695 towards ulvan were 0.43 mg·mL-1 and 0.11 μmol·min-1·mL-1, respectively. ESI-MS analysis showed that enzymatic products were mainly disaccharides and tetrasaccharides. This study reports a new PL25 family ulvan lyase, ALT3695, with properties that suggest its great potential for the preparation of ulvan oligosaccharides.
journal_name
Mar Drugsjournal_title
Marine drugsauthors
Gao J,Du C,Chi Y,Zuo S,Ye H,Wang Pdoi
10.3390/md17100568subject
Has Abstractpub_date
2019-10-08 00:00:00issue
10issn
1660-3397pii
md17100568journal_volume
17pub_type
杂志文章相关文献
Marine Drugs文献大全abstract::Marine natural products (MNPs) continue to be in the spotlight in the global drug discovery endeavor. Currently, more than 30,000 structurally diverse secondary metabolites from marine sources have been isolated, making MNPs a profound, renewable source to investigate novel drug compounds. Marine sponges of the genus ...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17020115
更新日期:2019-02-12 00:00:00
abstract::Fucoxanthin is a marine carotenoid exhibiting several health benefits. The anti-cancer effect of fucoxanthin and its deacetylated metabolite, fucoxanthinol, is well documented. In view of its potent anti-carcinogenic activity, the need to understand the underlying mechanisms has gained prominence. Towards achieving th...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md11125130
更新日期:2013-12-16 00:00:00
abstract::The marine environment is host to unparalleled biological and chemical diversity, making it an attractive resource for the discovery of new therapeutics for a plethora of diseases. Compounds that are extracted from cyanobacteria are of special interest due to their unique structural scaffolds and capacity to produce p...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md14010017
更新日期:2016-01-12 00:00:00
abstract::Ovarian cancer is one of the prevalent gynecological cancers occurring in women. In particular, the efficiency of standard therapeutic methods decreases when recurrence and chemoresistance ensue. To assist standard anti-cancer agents in the cure of ovarian cancer, development and application of new compounds such as s...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17060331
更新日期:2019-06-03 00:00:00
abstract::Two new sesterterpenes analogs, namely, 12-acetoxy,16-epi-hyrtiolide (1) and 12β-acetoxy,16β-methoxy,20α-hydroxy-17-scalaren-19,20-olide (2), containing a scalarane-based framework along with seven previously reported scalarane-type sesterterpenes (3-9) have been isolated from the sponge Hyrtios erectus (order Dictyoc...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md14070130
更新日期:2016-07-08 00:00:00
abstract::Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 μg/mL. In addition...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md9071187
更新日期:2011-01-01 00:00:00
abstract::The cultivation of macroalgae to supply the biofuel, pharmaceutical or food industries generates a considerable amount of organic residue, which represents a potential substrate for biomethanation. Its use optimizes the total resource exploitation by the simultaneous disposal of waste biomaterials. In this study, we e...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md13095947
更新日期:2015-09-18 00:00:00
abstract::Five new sulfide diketopiperazine derivatives, namely, penicibrocazines A-E (1-5), along with a known congener (6), were isolated and identified from the culture extract of Penicillium brocae MA-231, an endophytic fungus obtained from the fresh tissue of the marine mangrove plant Avicennia marina. The structures of th...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md13010276
更新日期:2015-01-07 00:00:00
abstract::Spirotetronates are actinomyces-derived polyketides that possess complex structures and exhibit potent and unexplored bioactivities. Due to their anticancer and antimicrobial properties, they have potential as drug hits and deserve further study. In particular, abyssomicin C and tetrocarcin A have shown significant pr...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md17040232
更新日期:2019-04-19 00:00:00
abstract::For a long time, fucoidan has been well known for its pharmacological activities, and recently low molecular weight fucoidan (LMF) has been used in food supplements and pharmaceutical products. In the present study, LMF was extracted from Laminaria japonica by enzyme hydrolysis. The toxicity of LMF in mouse and rat mo...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md14070121
更新日期:2016-06-24 00:00:00
abstract::Carotenoids are natural pigments that contribute to light harvesting and photo-protection in photosynthetic organisms. In this study, we analyzed the carotenoid profiles, including mono-hydroxy and epoxy-carotenoids, in the economically valuable red seaweed Pyropia yezoensis, to clarify the detailed biosynthetic and m...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16110426
更新日期:2018-11-01 00:00:00
abstract::In 2011, a Diarrhetic Shellfish Poisoning (DSP) outbreak occurred in British Columbia (BC), Canada that was associated with cooked mussel consumption. This is the first reported DSP outbreak in BC. Investigation of ill individuals, traceback of product and laboratory testing for toxins were used in this investigation....
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md11051669
更新日期:2013-05-21 00:00:00
abstract::24-methyl-cholesta-5,24(28)-diene-3β,19-diol-7β-monoacetate (MeCDDA) is a natural steroid compound isolated from a wild-type soft coral (Nephthea erecta). The present study aimed to investigate the anti-small cell lung cancer (SCLC) effects of MeCDDA in vitro and in vivo, as well as to elucidate its underlying mechani...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md15070210
更新日期:2017-07-01 00:00:00
abstract::Chitosan is the N-deacetylated derivative of chitin, a naturally abundant mucopolysaccharide that consists of 2-acetamido-2-deoxy-β-d-glucose through a β (1→4) linkage and is found in nature as the supporting material of crustaceans, insects, etc. Chitosan has been strongly recommended as a suitable functional materia...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md12115619
更新日期:2014-11-24 00:00:00
abstract::The marine alga, Symphyocladia latiuscula (Harvey) Yamada, is a good source of bromophenols with numerous biological activities. This study aims to characterize the anti-diabetic potential of 2,3,6-tribromo-4,5-dihydroxybenzyl derivatives isolated from S. latiuscula via their inhibition of tyrosine phosphatase 1B (PTP...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17030166
更新日期:2019-03-14 00:00:00
abstract::Cyclic imine toxins are neurotoxic, macrocyclic compounds produced by marine dinoflagellates. Mass spectrometric screenings of extracts from natural plankton assemblages revealed a high chemical diversity among this toxin class, yet only few toxins are structurally known. Here we report the structural characterization...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16110446
更新日期:2018-11-14 00:00:00
abstract::Mere15 is a novel polypeptide from Meretrix meretrix Linnaeus with cytotoxicity in solid cancer cells. In this study, we investigated its activity on human K562 chronic myelogenous leukemia cells. Mere15 inhibited the growth of K562 cells with IC₅₀ values of 38.2 μg/mL. Mere15 also caused concentration dependent induc...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md10112596
更新日期:2012-11-21 00:00:00
abstract::Two new diphenyl ethers (1 and 2) and four new phenolic bisabolane sesquiterpenoids (3⁻6), together with five known related derivatives, were isolated from the culture of the endophytic fungus Aspergillus flavus QQSG-3 obtained from a fresh branch of Kandelia obobata, which was collected from Huizhou city in the provi...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16090307
更新日期:2018-09-01 00:00:00
abstract::Collagen is the major fibrillar protein in most living organisms. Among the different types of collagen, type I collagen is the most abundant one in tissues of marine invertebrates. Due to the health-related risk factors and religious constraints, use of mammalian derived collagen has been limited. This triggers the s...
journal_title:Marine drugs
pub_type: 杂志文章,评审
doi:10.3390/md18090471
更新日期:2020-09-18 00:00:00
abstract::A series of novel cyclic marinopyrroles were designed and synthesized. Their activity to disrupt the binding of the pro-apoptotic protein, Bim, to the pro-survival proteins, Mcl-1 and Bcl-x(L), was evaluated using ELISA assays. Both atropisomers of marinopyrrole A (1) show similar potency. A tetrabromo congener 9 is t...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md12031335
更新日期:2014-03-07 00:00:00
abstract::Fucosterol is an algae-derived unique phytosterol having several medicinal properties, including antioxidant, anti-inflammatory, anticholinesterase, neuroprotective, and so on. Accumulated evidence suggests a therapeutic promise of fucosterol in neurodegeneration; however, the in-depth pharmacological mechanism of its...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17110639
更新日期:2019-11-13 00:00:00
abstract::The methods to obtain chitooligosaccharides are tightly related to the physicochemical properties of the end products. Knowledge of these physicochemical characteristics is crucial to describing the biological functions of chitooligosaccharides. Chitooligosaccharides were prepared either in a single-step enzymatic hyd...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md16110430
更新日期:2018-11-02 00:00:00
abstract::In this study, we aimed to illustrate the potential bio-effects of 3-bromo-4,5-dihydroxybenzaldehyde (3-BDB) on the antioxidant/cytoprotective enzyme heme oxygenase-1 (HO-1) in keratinocytes. The antioxidant effects of 3-BDB were examined via reverse transcription PCR, Western blotting, HO-1 activity assay, and immuno...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17040234
更新日期:2019-04-19 00:00:00
abstract::Fish processing industries generate a large volume of discards. In order to fulfil with the principles of a sustainable circular economy, it is necessary to maintain aquaculture by-products in the food chain through the production of high-value biomolecules that can be used as novel ingredients. In this study, we try ...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md18020101
更新日期:2020-02-04 00:00:00
abstract::Hepatic fibrosis is an effusive wound healing process, characterized by an excessive deposition of extracellular matrix (ECM), as the consequence of chronic liver injury of any etiology. Current therapeutic repertoire for hepatic fibrosis is limited to withdrawal of the noxious agent, which is not always feasible. Hen...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md13085201
更新日期:2015-08-18 00:00:00
abstract::Two new piperazine-triones lansai E and F (1, 2), together with four known secondary metabolites lansai D (3), 1-N-methyl-(E,Z)-albonoursin (4), imidazo[4,5-e]-1,2,4-triazine (5), and streptonigrin (6) were isolated from a deep-sea-derived Streptomycetes sp. strain SMS636. The structures of the isolated compounds were...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17030186
更新日期:2019-03-21 00:00:00
abstract::A glycopeptide fraction (GPF) from internal organs of green sea urchins (Strongylocentrotus droebachiensis Müller, Strongylocentrotidae) has been reported to be an effective bronchitis treatment. In this study, we evaluated the pharmacokinetic and tissue distribution of GPF, following single and repeated intranasal (i...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md17100577
更新日期:2019-10-11 00:00:00
abstract::In this study, we elucidated the inhibitory effect of fucoidan from marine brown algae on the lipid accumulation in differentiated 3T3-L1 adipocytes and its mechanism. The treatment of fucoidan in a dose-dependent manner was examined on lipid inhibition in 3T3-L1 cells by using Oil Red O staining. Fucoidan showed high...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md9081359
更新日期:2011-01-01 00:00:00
abstract::Marine cyanobacteria, notably those from tropical regions, are a rich source of bioactive secondary metabolites. Tropical marine cyanobacteria often grow to high densities in the environment, allowing direct isolation of many secondary metabolites from field-collected material. However, in temperate environments cultu...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md11041316
更新日期:2013-04-22 00:00:00
abstract::Cyclic imines are lipophilic marine toxins that bioaccumulate in seafood. Their structure comprises a cyclic-imino moiety, responsible for acute neurotoxicity in mice. Cyclic imines have not been linked yet to human poisonings and are not regulated in Europe, although the European Food Safety Authority requires more d...
journal_title:Marine drugs
pub_type: 杂志文章
doi:10.3390/md12063706
更新日期:2014-06-23 00:00:00