Abstract：
:Isoamylase catalyzes the hydrolysis of α-1,6-glycosidic linkages in glycogen, amylopectin and α/β-limit dextrins. A semi-rational design strategy was performed to improve catalytic properties of isoamylase from Bacillus lentus. Three residues in vicinity of the essential residues, Arg505, Asn513, and Gly608, were chosen as the mutation sites and were substituted by Ala, Pro, Glu, and Lys, respectively. Thermal stability of the mutant R505P and acidic stability of the mutant R505E were enhanced. The k cat /K m values of the mutant G608V have been promoted by 49%, and the specific activity increased by 33%. This work provides an effective strategy for improving the catalytic activity and stability of isoamylase, and the results obtained here may be useful for the improvement of catalytic properties of other α/β barrel enzymes.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Li Y,Zhang L,Ding Z,Gu Z,Shi Gdoi
10.1007/s10295-015-1708-4subject
Has Abstractpub_date
2016-01-01 00:00:00pages
3-12issue
1eissn
1367-5435issn
1476-5535pii
10.1007/s10295-015-1708-4journal_volume
43pub_type
杂志文章abstract：:Two anaerobic fungi, one a monocentric strain ( Piromyces sp. KSX1) and the other a polycentric strain ( Orpinomyces sp. 478P1), were immobilised in calcium alginate beads and cultured in sequential batches where spent medium (containing 0.25% cellobiose) was repeatedly drained and replaced. beta-Glucosidase productio...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-003-0033-5
更新日期：2003-04-01 00:00:00
abstract：:A newly isolated strain Penicillium sp. GXU20 produced a raw starch-degrading enzyme which showed optimum activity towards raw cassava starch at pH 4.5 and 50 °C. Maximum raw cassava starch-degrading enzyme (RCSDE) activity of 20 U/ml was achieved when GXU20 was cultivated under optimized conditions using wheat bran (...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-010-0910-7
更新日期：2011-06-01 00:00:00
abstract：:The production of recombinant proteins by Pichia pastoris under AOX1 promoter is usually performed using methanol together with either glycerol or sorbitol as co-substrate. Although both co-substrates have been widely used, comparative studies are scarce. In addition, these comparisons have been performed at different...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-016-1895-7
更新日期：2017-03-01 00:00:00
abstract：:Corynebacterium glutamicum SNK 118 was metabolically engineered with improved L-arginine titer. Considering the crucial role of NADPH level in L-arginine production, pntAB (membrane-bound transhydrogenase) and ppnK (NAD+ kinase) were co-expressed to increase the intracellular NADPH pool. Expression of pntAB exhibited ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-018-2103-8
更新日期：2019-01-01 00:00:00
abstract：:Isoprenoids are a large class of natural products with myriad applications as bioactive and commercial compounds. Their diverse structures are derived from the biosynthetic assembly and tailoring of their scaffolds, ultimately constructed from two C5 hemiterpene building blocks. The modular logic of these platforms ca...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-020-02306-3
更新日期：2020-10-01 00:00:00
abstract：:Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-008-0403-0
更新日期：2008-12-01 00:00:00
abstract：:Bacterial contamination of commercial fermentation cultures is a common and costly problem to the fuel ethanol industry. Antimicrobials such as virginiamycin (VIR) and penicillin (PEN) are frequently used to control contamination but there are little data available on the susceptibility of bacterial contaminants to th...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-007-0250-4
更新日期：2007-11-01 00:00:00
abstract：:Component C (Acarviosy-1,4-Glc-1,1-Glc) was a highly structural acarbose analog, which could be largely formed during acarbose fermentation process, resulting in acarbose purification being highly difficult. By choosing osmolality level as the key fermentation parameter of acarbose-producing Actinoplanes sp. A56, this...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1520-6
更新日期：2014-12-01 00:00:00
abstract：:Cyanobacteria are commonly used for the phytostimulation and biofertilization of agriculture crops due to their nitrogen-fixing ability. However, the contribution by their phytohormones has been neglected. This study focuses on the screening of rhizospheric and free-living cyanobacteria for in vitro phytohormones prod...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-010-0833-3
更新日期：2011-01-01 00:00:00
abstract：:Bear bile powder is a precious medicinal material. It is characterized by high content of tauroursodeoxycholic acid (TUDCA) at a ratio of 1.0-1.5 to taurochenodeoxycholic acid (TCDCA). Here, we reported the biotransformation of tauroursodeoxycholic acid (TUDCA) through Escherichia coli engineered with a two-step mimic...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-017-1935-y
更新日期：2017-07-01 00:00:00
abstract：:Rapamycin is an immunosuppressive metabolite produced from several actinomycete species. Besides its immunosuppressive activity, rapamycin and its analogs have additional therapeutic potentials, including antifungal, antitumor, neuroprotective/neuroregenerative, and lifespan extension activities. The core structure of...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-016-1834-7
更新日期：2017-05-01 00:00:00
abstract：:L-DOPA is a key pharmaceutical agent for treating Parkinson's, and market demand has exploded due to the aging population. There are several challenges associated with the chemical synthesis of L-DOPA, including complicated operation, harsh conditions, and serious pollution. A biocatalysis route for L-DOPA production ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-019-02237-8
更新日期：2019-12-01 00:00:00
abstract：:The acidification behavior of Lactobacillus bulgaricus and Streptococcus thermophilus for yoghurt production was investigated along temperature profiles within the optimal window of 38-44 degrees C. For the optimal acidification temperature profile search, an optimization engine module built on a modular artificial ne...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-007-0220-x
更新日期：2007-07-01 00:00:00
abstract：:Fabclavines, unusual peptide-polyketide-polyamine hybrids, show broad-spectrum bioactivity against a variety of different organism like Gram-positive and -negative bacteria, fungi and protozoa. We elucidated the biosynthesis of these NRPS-PKS hybrids in Xenorhabdus szentirmaii by deletion of most genes encoded in the ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-018-02124-8
更新日期：2019-03-01 00:00:00
abstract：:Denitrification is one of the key processes of the global nitrogen (N) cycle driven by bacteria. It has been widely known for more than 100 years as a process by which the biogeochemical N-cycle is balanced. To study this process, we develop an individual-based model called INDISIM-Denitrification. The model embeds a ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-019-02245-8
更新日期：2020-01-01 00:00:00
abstract：:A number of experiments was conducted in order to establish if N(2)O in the exhaust gas from an aerobic consortium of nitrifiers could be used as an indicator for monitoring the nitrification process. Laboratory-scale experiments with an activated sludge system showed a strong correlation between ammonia shock loads a...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000286
更新日期：2002-12-01 00:00:00
abstract：:Several unique Sus-like polysaccharide utilization loci (PULs) were identified from bacteria resident in bovine rumen microbiomes through functional screening of a fosmid library. The loci were phylogenetically assigned to the genus Prevotella within the phylum Bacteroidetes. These findings were augmented by a bioinfo...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-013-1395-y
更新日期：2014-03-01 00:00:00
abstract：:The use of proteomics for direct detection of expressed pathways producing natural products has yielded many new compounds, even when used in a screening mode without a bacterial genome sequence available. Here we quantify the advantages of having draft DNA-sequence available for strain-specific proteomics using the l...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-013-1373-4
更新日期：2014-02-01 00:00:00
abstract：:Two on-line probes for biomass measurement in bioreactor cultivations were evaluated. One probe is based on near infrared (NIR) light absorption and the other on dielectric spectroscopy. The probes were used to monitor biomass production in cultivations of several different microorganisms. Differences in NIR probe res...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-007-0233-5
更新日期：2007-08-01 00:00:00
abstract：:Nine thraustochytrid strains isolated from subtropical mangroves were screened for their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production potential in a glucose yeast extract medium. Their ability to utilize okara (soymilk residue) for growth and EPA and DHA production was also evaluated. EPA yiel...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000169
更新日期：2001-10-01 00:00:00
abstract：:Acetoin, a major extracellular catabolic product of Bacillus subtilis cultured on glucose, is widely used to add flavor to food and also serves as a precursor for chemical synthesis. The biosynthesis of acetoin from pyruvate requires the enzymes α-acetolactate synthase (ALS) and α-acetolactate decarboxylase (ALDC), bo...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-013-1303-5
更新日期：2013-09-01 00:00:00
abstract：:Liquid phase oxidation process using chelated iron solution is among the most promising techniques for the hydrogen sulfide removal due to its double advantage of waste minimization and resource recovery. Regeneration of chelated iron is a core reaction in this process. Regeneration of chelated iron in acidic solution...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1510-8
更新日期：2014-11-01 00:00:00
abstract：:As a part of our ongoing efforts towards finding novel antimycotic agents from marine microflora of the Red Sea, vanillin, 5,7-dimethoxy-4-p-methoxylphenylcoumarin and the new antimycotic compound saadamycin were isolated from endophytic Streptomyces sp. Hedaya48. The producing strain was isolated from the Egyptian sp...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-010-0729-2
更新日期：2010-08-01 00:00:00
abstract：:Microbial diversity is fundamental to maintenance and conservation of global genetic resources. As extreme environments are explored, the richness of microbial diversity is increasingly evident. Measures must be taken to estimate, record, and conserve microbial diversity, not only to sustain human health but also to e...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1038/sj.jim.2900390
更新日期：1997-05-01 00:00:00
abstract：:Development of natural products for therapeutic use is often hindered by limited availability of material from producing organisms. The speed at which current technologies enable the cloning, sequencing, and manipulation of secondary metabolite genes for production of novel compounds has made it impractical to optimiz...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-003-0045-1
更新日期：2003-08-01 00:00:00
abstract：:For decades, microbial natural products have been one of the major sources of novel drugs for pharmaceutical companies, and today all evidence suggests that novel molecules with potential therapeutic applications are still waiting to be discovered from these natural sources, especially from actinomycetes. Any appropri...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s10295-010-0882-7
更新日期：2011-03-01 00:00:00
abstract：:Since the discovery of the first antibiotic, natural products have played an important role in chemistry, biology and medicine. To explore the potential of bioactive compounds from microbes isolated from the southeast of Tibet, China, a crude extract library was constructed and screened against Staphylococcus aureus. ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-017-1908-1
更新日期：2017-05-01 00:00:00
abstract：:A membrane-screening method was developed in conjunction with flow cytometric (FC) analysis for determining the efficacies of antimould pressure-treatment formulations for mould species of cosmetic significance on southern pine. Fusarium subglutinans, Aspergillus flavus, Penicillium chrysogenum, and Paecilomyces spp. ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000279
更新日期：2002-12-01 00:00:00
abstract：:Production of recombinant proteins is an industrially important technique in the biopharmaceutical sector. Many recombinant proteins are problematic to generate in a soluble form in bacteria as they readily form insoluble inclusion bodies. Recombinant protein solubility can be enhanced by minimising stress imposed on ...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-014-1489-1
更新日期：2014-09-01 00:00:00
abstract：:Streptomyces transglutaminase (TGase) has been widely used in food, pharmaceutical and textile industries. Streptomyces TGase is naturally synthesized as zymogen (pro-TGase), which is then processed to produce active enzyme by removing its N-terminal pro-peptide. Although the pro-peptide is essential for TGase folding...
journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1007/s10295-012-1221-y
更新日期：2013-04-01 00:00:00
