Abstract：
:The use of proteomics for direct detection of expressed pathways producing natural products has yielded many new compounds, even when used in a screening mode without a bacterial genome sequence available. Here we quantify the advantages of having draft DNA-sequence available for strain-specific proteomics using the latest in ultrahigh-resolution mass spectrometry for both proteins and the small molecules they generate. Using the draft sequence of Streptomyces lilacinus NRRL B-1968, we show a >tenfold increase in the number of peptide identifications vs. using publicly available databases. Detected in this strain were six expressed gene clusters with varying homology to those known. To date, we have identified three of these clusters as encoding for the production of griseobactin (known), rakicidin D (an orphan NRPS/PKS hybrid cluster), and a putative thr and DHB-containing siderophore produced by a new non-ribosomal peptide sythetase gene cluster. The remaining three clusters show lower homology to those known, and likely encode enzymes for production of novel compounds. Using an interpreted strain-specific DNA sequence enables deep proteomics for the detection of multiple pathways and their encoded natural products in a single cultured bacterium.
journal_name
J Ind Microbiol Biotechnoljournal_title
Journal of industrial microbiology & biotechnologyauthors
Albright JC,Goering AW,Doroghazi JR,Metcalf WW,Kelleher NLdoi
10.1007/s10295-013-1373-4subject
Has Abstractpub_date
2014-02-01 00:00:00pages
451-9issue
2eissn
1367-5435issn
1476-5535journal_volume
41pub_type
杂志文章abstract：:A metabolic network consisting of 48 reactions was established to describe intracellular processes during growth and poly-3-hydroxybutyrate (PHB) production for Cupriavidus necator DSM 545. Glycerol acted as the sole carbon source during exponential, steady-state cultivation conditions. Elementary flux modes were obta...
journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2014-12-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2011-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2006-07-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2011-09-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章
doi：10.1038/sj.jim.7000286
更新日期：2002-12-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,已发布勘误
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更新日期：2018-06-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-011-0981-0
更新日期：2011-12-01 00:00:00
abstract：:Acid blue-15, a complex and resonance-stabilized triphenylmethane (TPM) textile dye, resistant to transformation, was decolorized/degraded in an up-flow immobilized cell bioreactor. A consortium comprised of isolates belonging to Bacillus sp., Alcaligenes sp. and Aeromonas sp. formed a multispecies biofilm on refracto...
journal_title：Journal of industrial microbiology & biotechnology
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doi：10.1007/s10295-004-0121-1
更新日期：2004-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2009-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,评审
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更新日期：2010-03-01 00:00:00
abstract：:Ethyl carbamate (EC), a pluripotent carcinogen, is mainly formed by a spontaneous chemical reaction of ethanol with urea in wine. The arginine, one of the major amino acids in grape musts, is metabolized by arginase (encoded by CAR1) to ornithine and urea. To reduce the production of urea and EC, an arginase-deficient...
journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2016-05-01 00:00:00
abstract：:The fruity odor of Chinese liquor is largely derived from ester formation. Ethyl caproate, an ethyl ester eliciting apple-like flavor, is one of the most important esters in the strong aromatic Chinese liquor (or Luzhou-flavor liquor), which is the most popular and best-selling liquor in China. In the traditional ferm...
journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2014-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2020-01-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
pub_type： 杂志文章,收录出版
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更新日期：2017-02-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2010-03-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2016-08-01 00:00:00
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journal_title：Journal of industrial microbiology & biotechnology
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更新日期：2013-09-01 00:00:00
