Abstract:
OBJECTIVE:Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) are responsible for a variety of human diseases. Therefore, rapid detection of these viruses in clinical laboratory is important. Recently Aptima Herpes Simplex 1 and 2 assay has been approved by the FDA. We evaluated analytical performance of this assay by comparing results obtained by analysis of cultures. MATERIALS AND METHODS:Aptima Herpes Simplex assay is a nucleic acid amplification test that can be fully automated using the Hologic Panther instrument. This is a qualitative test providing either positive or negative result. We analyzed 115 specimens collected from anogenital locations using the new Aptima Herpes Simplex assay and our current tissue culture method. RESULTS:We observed good correlation between results obtained by using Aptima assay and the tissue culture method in 101 specimens but 14 specimens showed discordant results. Further testing in a reference laboratory using PCR (polymerase chain reaction) showed results in agreement with the Aptima assay but not the tissue culture method. The cause of discordance was misidentification of HSV-2 as HSV-1 by the tissue culture method. CONCLUSIONS:Aptima Herpes simplex assay on the Hologic Panther instrument is suitable for rapid detection of herpes simplex virus in clinical laboratory.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Dixon RB,Bonaparte J,Greenwood CJ,Davis M,Dasgupta Asubject
Has Abstractpub_date
2020-03-01 00:00:00pages
278-281issue
2eissn
0091-7370issn
1550-8080pii
50/2/278journal_volume
50pub_type
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journal_title:Annals of clinical and laboratory science
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journal_title:Annals of clinical and laboratory science
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