Abstract:
Background:Currently, effective genetic markers are limited to predict the clinical outcome of melanoma. High-throughput multiomics sequencing data have provided a valuable approach for the identification of genes associated with cancer prognosis. Method:The multidimensional data of melanoma patients, including clinical, genomic, and transcriptomic data, were obtained from The Cancer Genome Atlas (TCGA). These samples were then randomly divided into two groups, one for training dataset and the other for validation dataset. In order to select reliable biomarkers, we screened prognosis-related genes, copy number variation genes, and SNP variation genes and integrated these genes to further select features using random forests in the training dataset. We screened for robust biomarkers and established a gene-related prognostic model. Finally, we verified the selected biomarkers in the test sets (GSE19234 and GSE65904) and on clinical samples extracted from melanoma patients using qRT-PCR and immunohistochemistry analysis. Results:We obtained 1569 prognostic-related genes and 1101 copy-amplification, 1093 copy-deletions, and 92 significant mutations in genomic variants. These genomic variant genes were closely related to the development of tumors and genes that integrate genomic variation. A total of 141 candidate genes were obtained from prognosis-related genes. Six characteristic genes (IQCE, RFX6, GPAA1, BAHCC1, CLEC2B, and AGAP2) were selected by random forest feature selection, many of which have been reported to be associated with tumor progression. Cox regression analysis was used to establish a 6-gene signature. Experimental verification with qRT-PCR and immunohistochemical staining proved that these selected genes were indeed expressed at a significantly higher level compared with the normal tissues. This signature comprised an independent prognostic factor for melanoma patients. Conclusions:We constructed a 6-gene signature (IQCE, RFX6, GPAA1, BAHCC1, CLEC2B, and AGAP2) as a novel prognostic marker for predicting the survival of melanoma patients.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Gao Y,Li Y,Niu X,Wu Y,Guan X,Hong Y,Chen H,Song Bdoi
10.1155/2020/5323614subject
Has Abstractpub_date
2020-05-08 00:00:00pages
5323614eissn
2314-6133issn
2314-6141journal_volume
2020pub_type
杂志文章abstract::This study investigated how the posture of the thumb while performing common pinch movements and the levels of pinch force applied by the thumb affect the arthrokinematics of the trapeziometacarpal joint in vivo. Fifteen subjects performed the pinch tasks at the distal phalange (DP), proximal interphalangeal (PIP) joi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/157295
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:To estimate localization and the period up to the appearance of small hyperreflective round-shaped droplets using spectral-domain optical coherence tomography (SD-OCT) after pars plana vitrectomy with silicone oil tamponade. METHODS:A retrospective observational study included 24 patients who had undergone par...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/372045
更新日期:2014-01-01 00:00:00
abstract::The aim of this study was to investigate the efficacy of the sequential combined 585 nm PDL and the 1064 nm neodymium:yttrium-aluminium-garnet laser (PDL/Nd:YAG) in the treatment of surgical scars and to evaluate the short-term effects by in vivo confocal microscopy (RCM) and the long-term effects by clinical assessme...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/204532
更新日期:2014-01-01 00:00:00
abstract:Aim:To address the impact of noncavity-distorting intramural fibroids on the efficacy of in vitro fertilization-embryo transfer (IVF-ET) outcomes. Methods:The PubMed, Web of Science, Embase, Cochrane Library, and China National Knowledge Infrastructure were searched systematically. A meta-analysis was performed based ...
journal_title:BioMed research international
pub_type: 杂志文章,meta分析
doi:10.1155/2018/8924703
更新日期:2018-09-04 00:00:00
abstract::The establishment of the subgingival microbiota is dependent on successive colonization of the implant surface by bacterial species. Different implant surface topographies could influence the bacterial adsorption and therefore jeopardize the implant survival. This study evaluated the biofilm formation capacity of five...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/159625
更新日期:2015-01-01 00:00:00
abstract::Platelets are anuclear cells and are devoid of genomic DNA, but they are capable of de novo protein synthesis from mRNA derived from their progenitor cells, megakaryocytes. There is mounting evidence that microRNA (miRNA) plays an important role in regulating gene expression in platelets. miR-223 is the most abundant ...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/981841
更新日期:2015-01-01 00:00:00
abstract::Human cytoplasmic alpha-fetoprotein (AFP) has been classified as a member of the albuminoid gene family. The protein sequence of AFP has significant homology to that of human serum albumin (HSA), but its biological characteristics are vastly different from HSA. The AFP functions as a regulator in the phosphatidylinosi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/256916
更新日期:2015-01-01 00:00:00
abstract:Background:Osteoarthritis (OA) is a common disease characterized by articular cartilage degeneration and secondary hyperosteogenesis. Genetic factors are associated with the occurrence of OA. While several studies have shown that the matrix metalloproteinase-1- (MMP-1-) 1607 1G/2G (rs1799750) polymorphism may be relate...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/5190587
更新日期:2020-05-20 00:00:00
abstract::A new fungus Cladosporium oxysporum GQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence. C. oxysporum produced maximum xylanase activity of 55.92 U/mL with wheat bran as a subst...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/4575024
更新日期:2016-01-01 00:00:00
abstract::Objectives. Osteopontin (OPN) is overexpressed in breast cancers, while its clinical and prognostic significance remained unclear. This study aimed to assess the prognostic value of OPN, especially its splice variants, in breast cancers. Methods. Data were extracted from eligible studies concerning the OPN and OPN-c e...
journal_title:BioMed research international
pub_type: 杂志文章,meta分析
doi:10.1155/2016/7310694
更新日期:2016-01-01 00:00:00
abstract::Pregnancy is associated with various physiological changes which may lead to significant alterations in the pharmacokinetics of many drugs. The present study was aimed to investigate the potential effects of pregnancy on the pharmacokinetic profile of zonisamide (ZNM) in the rabbit. Seven female rabbits were used in t...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/140327
更新日期:2013-01-01 00:00:00
abstract::Although studies have shown that human migration is one of the risk factors for the spread of drug-resistant organisms such as methicillin-resistant Staphylococcus aureus (MRSA), surveillance studies examining MRSA among refugee populations in the US are lacking. This study aimed to assess the prevalence and molecular...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/5739247
更新日期:2019-07-01 00:00:00
abstract::Destruction of extracellular matrix (ECM) is one of the basic steps of tumor invasion and metastasis. Matrix metalloproteinase (MMP) 9, a kind of zinc-ion-dependent endopeptidase, can degrade almost all protein components in the ECM, destroy the histological barrier of tumor cell invasion, and play a key role in tumor...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4350783
更新日期:2020-04-15 00:00:00
abstract::Ceramic materials show excellent esthetic behavior, along with an absence of hypersensitivity, making them a possible alternative implant material in dental surgery. However, their surface properties enable only limited osseointegration compared to titanium implants. Within this study, a novel surface coating techniqu...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/742180
更新日期:2014-01-01 00:00:00
abstract:AIM:Gastrointestinal mucositis is a frequent complication of antineoplastic chemotherapy, but the effects of chemotherapy on mucosal defense mechanisms remain poorly understood. We studied the effects of cisplatin on mucin, one of the principal defense factors of the gastrointestinal mucosa, and evaluated the efficacy ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/276186
更新日期:2013-01-01 00:00:00
abstract:AIMS:Nausea and vomiting of pregnancy is the most common medical condition in pregnancy. There is an increasing trend to prescribe ondansetron although its safety for use in pregnancy has not been established. METHODS:Exposed pregnancies were all births in Western Australia, 2002-2005, where the mother was dispensed o...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/909860
更新日期:2013-01-01 00:00:00
abstract::Molecular epidemiology has revealed that Mycobacterium tuberculosis (Mtb), formerly regarded as highly conserved species, displays a considerable degree of genetic variability that can influence the outcome of the disease as well as the innate and adaptive immune response. Recent studies have demonstrated that Mtb fam...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/741309
更新日期:2014-01-01 00:00:00
abstract::Malignant melanoma is characterized by its bad prognosis for aggressiveness, drug resistance, and early metastasis. Isorhamnetin (3'-methoxy-3,4',5,7-tetrahydroxyflavone; IH) is a natural flavonoid that has been investigated for its antitumor effects in breast cancer, colon cancer, and gastric cancer through inducing ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/1057943
更新日期:2020-05-05 00:00:00
abstract::This study aims to evaluate whether diets containing mixture of formic and propionic acid alone or supplemented with either capric acid or Bacillus Licheniformis can alleviate immune inflammatory response of piglets challenged with enterotoxigenic Escherichia coli (ETEC). A total of 30 weaning pigs were assigned to 5 ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/6416187
更新日期:2019-03-25 00:00:00
abstract::Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half-life of few minutes. In order to prolong their activity, we have conjugated to iron oxide nanoparticles three neurotrophic factors: nerve...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/267808
更新日期:2014-01-01 00:00:00
abstract::The epilepsies are a heterogeneous group of neurological disorders and syndromes characterised by recurrent, involuntary, paroxysmal seizure activity, which is often associated with a clinicoelectrical correlate on the electroencephalogram. The diagnosis of epilepsy is usually made by a neurologist but can be difficul...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/986736
更新日期:2015-01-01 00:00:00
abstract::Asthma susceptibility genes are mapped to a region on human chromosome 5q31-q33, which contains a cluster of proinflammatory cytokine genes such as interleukin-13 (IL-13), which is associated with asthma. This study investigated the allele frequencies of two single nucleotide polymorphisms (SNPs) (-1111C>T and 4257C>A...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/981012
更新日期:2013-01-01 00:00:00
abstract::Diesel exhaust and its particles (DEP) have been under scrutiny for health effects in humans. In the development of these effects inflammation is regarded as a key process. Overall, in vitro studies report similar DEP-induced changes in markers of inflammation, including cytokines and chemokines, as studies in vivo. I...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/685142
更新日期:2013-01-01 00:00:00
abstract::During hundreds of years of medication, it is believed that the steamed Panax notoginseng (SPN) can enrich and regulate the blood, replenish the body, and improve the health. The aim of this study was to optimize the steaming conditions of SPN which are related to the hematopoietic effect. In the study, network pharma...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9371426
更新日期:2020-07-26 00:00:00
abstract::Oxidative stress is a normal phenomenon in the body. Under normal conditions, the physiologically important intracellular levels of reactive oxygen species (ROS) are maintained at low levels by various enzyme systems participating in the in vivo redox homeostasis. Therefore, oxidative stress can also be viewed as an i...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/761264
更新日期:2014-01-01 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1) is glucose-dependent insulinotropic hormone secreted from enteroendocrine L cells. Its long-acting analogue, exendin-4, is equipotent to GLP-1 and is used to treat type 2 diabetes mellitus. In addition, exendin-4 has effects on the central and peripheral nervous system. In this study, w...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/315848
更新日期:2013-01-01 00:00:00
abstract:Introduction:The aim of this research was to test, in an animal model, the nerve regeneration technique with a hypoallergenic acellular dermal matrix used to wrap the microsurgical neural suture. Materials and Methods:Two groups of rats received the cut of limb right median nerves. The regeneration technique considers...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/4750624
更新日期:2019-06-17 00:00:00
abstract:Background:This study is aimed at identifying unknown clinically relevant genes involved in colorectal cancer using bioinformatics analysis. Methods:Original microarray datasets GSE107499 (ulcerative colitis), GSE8671 (colorectal adenoma), and GSE32323 (colorectal cancer) were downloaded from the Gene Expression Omnib...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/1204605
更新日期:2020-05-23 00:00:00
abstract::Hemoglobin, which widely exists in all vertebrates and in some invertebrates, is possibly a precursor of antimicrobial peptides (AMPs). However, AMPs in the hemoglobin of invertebrates have been rarely investigated. This study is the first to report the full-length cDNA, prokaryotic expression, and antimicrobial activ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/5683026
更新日期:2016-01-01 00:00:00
abstract:Background:Lung cancer is the leading cause of death worldwide. The treatment choice for advanced stage of lung cancer may depend on histotype, performance status (PS), age, and comorbidities. In the present study, we focused on the effect of metronomic vinorelbine treatment in elderly patients with advanced unresectab...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/6278403
更新日期:2018-08-27 00:00:00