Abstract:
:The aim of this study was to explore the effects of α-zearalanol (α-ZAL) on the proliferation of mouse bone-marrow-derived mesenchymal stem cells (BMSCs) and their differentiation into osteoblasts. Six- to eight-week-old BALB/C mice were used either as recipients or as bone marrow donors. BMSCs were isolated and collected using a differential adhesion method, with use of 10 % fetal bovine serum and Iscove's modified Dulbecco's medium. After the third generation, the BMSCs were randomly placed into the following subgroups: a control group, an osteogenic medium (OM) group, a 17β-estradiol group, an α-ZAL 10(-7) mol/L group, an α-ZAL 10(-6) mol/L group, and an α-ZAL 10(-5) mol/L group. Flow cytometry was used to identify the BMSCs collected from the bone marrow. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was performed, and markers of the osteoblasts were measured in the different subgroups. In addition, expression of osteoprotegerin and expression of receptor activator of nuclear factor κB ligand were examined using Western blot. In contrast to the control and OM groups, BMSCs in the α-ZAL groups exhibited long fusiform shapes, and contact inhibition was observed when the cells were closely packed. After induction, the BMSCs grew well and exhibited triangular, star, polygonal, or irregular shapes. Clumps and multiple cells were evident. The trends of the proliferation and differentiation for the control, OM, 17β-estradiol, and α-ZAL groups were similar. Compared with the control and OM groups, in the α-ZAL groups the expression levels of alkaline phosphatase, procollagen type I N-terminal propeptide, bone morphogenetic protein 2, and osteocalcin were significantly increased (p < 0.05). In addition, α-ZAL inhibited osteoclastogenesis by increasing the expression of osteoprotegerin and decreasing the expression of nuclear factor κB ligand. In conclusion, α-ZAL can increase the proliferation of BMSCs and their differentiation into osteoblasts and can effectively suppress osteoclastogenesis.
journal_name
J Bone Miner Metabjournal_title
Journal of bone and mineral metabolismauthors
Zong S,Zeng G,Fang Y,Peng J,Zou B,Gao T,Zhao Jdoi
10.1007/s00774-015-0659-1subject
Has Abstractpub_date
2016-03-01 00:00:00pages
151-60issue
2eissn
0914-8779issn
1435-5604pii
10.1007/s00774-015-0659-1journal_volume
34pub_type
杂志文章abstract::Histomorphometry is commonly applied to study bone remodeling. Histological definitions of cortical bone boundaries have not been consistent. In this study, new criteria for specific definition of the transitional zone between the cortical and cancellous bone in the femoral neck were developed. The intra- and inter-ob...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-014-0562-1
更新日期:2015-01-01 00:00:00
abstract::Hypophosphatasia is a rare inherited disorder characterized by a low serum alkaline phosphatase (ALP) activity and defective bone mineralization. Adult hypophosphatasia typically manifests in middle-age as a result of osteomalacia with recurrent stress fractures of the lower limb. However, considerable variation occur...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-011-0338-9
更新日期:2012-11-01 00:00:00
abstract::Osteoclastogenic cytokines produced by T and B lineage cells and interleukin (IL)-7-induced expansion of the pool size of osteoclast precursors have been suggested to play an important role in acceleration of osteoclastogenesis induced by estrogen deficiency. However, the contribution of increased RANKL produced by os...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-006-0723-y
更新日期:2007-01-01 00:00:00
abstract::In order to investigate the effect of manganese (Mn) deficiency on bone metabolism in chicks, ninety 1-day-old male Arbor Acre chicks were randomly divided into 3 groups and each group were given a diet having a different concentration of Mn (60 mg kg(-1), control group; 40 mg kg(-1), Mn-deficient group I; 8.7 mg kg(-...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-012-0417-6
更新日期:2013-05-01 00:00:00
abstract::Trabecular bone density at the distal radius and cortical bone density at the midradius were measured in four randomized groups of women before and after 4 months administration of AAACa, oyster shell heated under reduced pressure with addition of heated algal ingredient (HAI) (group A); AACa, the same preparation wit...
journal_title:Journal of bone and mineral metabolism
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s007740070022
更新日期:2000-01-01 00:00:00
abstract::Osteonecrosis of the jaw (ONJ) associated with bisphosphonate therapy is a rare but severe side effect in osteoporosis patients. Recently, the number of osteoporosis patients with ONJ has dramatically increased in Japan. This has contributed to an increase in the number of patients avoiding extractions. However, there...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00774-019-00990-5
更新日期:2019-09-01 00:00:00
abstract::Endoplasmic reticulum (ER) stress has been reported to be linked to various diseases such as diabetes, neurodegenerative diseases, and osteogenesis imperfecta (OI). Old astrocyte specifically induced substance (OASIS), a novel type of ER stress transducer, is a basic leucine zipper transcription factor belonging to th...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-009-0142-y
更新日期:2010-07-01 00:00:00
abstract::Secondary hyperparathyroidism (SHPT) develops as a compensatory mechanism when the body is in calcium deficit. SHPT may be harmful and has been associated with elevated blood pressure. The cause of SHPT could be low calcium intake, reduced intestinal calcium absorption, or increased excretion. However, the relative im...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-005-0647-y
更新日期:2006-01-01 00:00:00
abstract::Although the influence of weight-bearing activity on bone mass has been widely investigated in white women, few studies have been conducted in black, African populations. We investigated bone mineral density (BMD) in black South African women, with and without a history of load-carrying on the head. We also investigat...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-009-0113-3
更新日期:2010-03-01 00:00:00
abstract::Concurrent use of bisphosphonate therapy reduces the anabolic effect of teriparatide. Consequently, in clinical practice bisphosphonates are discontinued and teriparatide therapy held for a few months to allow bone turnover to increase. We aimed to evaluate the effect of prior bisphosphonate exposure and the effect of...
journal_title:Journal of bone and mineral metabolism
pub_type: 临床试验,杂志文章
doi:10.1007/s00774-009-0101-7
更新日期:2010-01-01 00:00:00
abstract::Osteopathic changes sometimes occur in patients with complex regional pain syndrome (reflex sympathetic dystrophy and causalgia). We aimed to investigate whether such osteopathic changes occurred in rats with chronic constriction injury (CCI) of the sciatic nerve. A CCI of the sciatic nerve was established in a unilat...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s007740200012
更新日期:2002-01-01 00:00:00
abstract:INTRODUCTION:This study aimed to examine long-term persistence in new users of oral bisphosphonates in a population-wide cohort in Manitoba, Canada. MATERIALS AND METHODS:A longitudinal observational study was conducted using administrative health data characterizing long-term bisphosphonate persistence in those who s...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-019-01069-x
更新日期:2020-05-01 00:00:00
abstract::Gastrectomy-evoked osteopenia in the femoral metaphysis of rats can be prevented by the consumption of fructooligosaccharides (FOS). We examined the effect of FOS on the femoral neck. Twenty-eight 5-week-old male Sprague-Dawley rats were divided into two groups, sham-operated (SH) and gastrectomized (GX). One week aft...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-003-0423-9
更新日期:2003-01-01 00:00:00
abstract::In this study, we focused on collagen metabolism as a factor involved in menatetrenone (MK-4)-related improvement in bone quality. Using rats with a congenital ascorbic acid (AA) deficiency, osteogenic disorder Shionogi (ODS) rats, we established a model in which abnormal collagen metabolism reduced bone mechanical pr...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-009-0069-3
更新日期:2009-01-01 00:00:00
abstract::Notch is a transmembrane protein involved in cell fate determination. In the present study, we observed temporally and spatially restricted expression of Notch1 in developing cartilage. Notch1 was localized starting from the mesenchymal condensation stage of embryonic mouse forelimbs. Interestingly, although localizat...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-003-0428-4
更新日期:2003-01-01 00:00:00
abstract::The activation sequence of clasts (the designation clast was used because ultrastructurally in this tissue, it is not always possible to differentiate between chondroclasts sitting on cartilage and osteoclasts sitting on bone matrix) was studied in vivo using the healing of low-phosphate, vitamin D-deficiency rickets ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-005-0626-3
更新日期:2005-01-01 00:00:00
abstract::Vertebral fracture (VFx) is associated with various co-morbidities and increased mortality. In this paper, we have studied the detective value of height loss for VFx using two indices; historical height loss (HHL) which is the difference between the maximal height, and the current height (CH), and CH/knee height (KH) ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-013-0525-y
更新日期:2014-09-01 00:00:00
abstract::There are many factors that increase the risk of osteoporosis, including smoking, malnutrition, vitamin D deficiency, hypogonadism, limited physical activity due to chronic disease, and corticosteroid therapy in chronic obstructive pulmonary disease (COPD). The aim of this study was to investigate bone mineral density...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-002-0416-0
更新日期:2003-01-01 00:00:00
abstract::We designed a randomized, double-blind, controlled clinical trial to compare the effect of two regimens for administering cholecalciferol on the serum 25-hydroxycholecalciferol [25(OH)D] levels and in the reversion of secondary hyperparathyroidism in the elderly living in a low-income housing unit in the city of Porto...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00774-008-0858-0
更新日期:2008-01-01 00:00:00
abstract::Various animal experiments and human studies have shown that intermittent injections of parathyroid hormone (PTH) exert anabolic effects on bone, whereas continuous PTH treatment decreases the bone mass and causes hypercalcemia in animals. However, limited data are available with regard to the effects of a repetitive ...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-010-0181-4
更新日期:2010-11-01 00:00:00
abstract::Metformin, an anti-hyperglycemic agent used for type 2 diabetes, has recently been found to have more effects apart from glucose regulation. We found that, in ultra-high-molecular-weight polyethylene particle-induced osteolysis mouse models, metformin had bone protect property and reduced the negative regulator of bon...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-018-0939-7
更新日期:2019-05-01 00:00:00
abstract::Menin, a product of the MEN1 gene, is related to the ontogeny of several cancers such as MEN1 and sporadic endocrine tumors, although it is considered to be a tumor suppressor. Many proteins interact with menin, and it is involved in various biological functions in several tissues. Menin plays some physiological and p...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章,评审
doi:10.1007/s00774-012-0355-3
更新日期:2012-07-01 00:00:00
abstract::Patients who are diagnosed with osteoporosis and beginning treatment often discontinue their osteoporosis medication relatively early after the start of treatment because of their poor recognition of fracture risk and the asymptomatic nature of osteoporosis. In this study we aimed to assess adherence to treatment with...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00774-009-0112-4
更新日期:2010-03-01 00:00:00
abstract::We performed this study to clarify whether celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, prevents trabecular bone mass reduction by suppressing arthritis-related increase of bone resorption, and to discriminate differences in actions on bone among celecoxib, SC-58560 (a selective COX-1 inhibitor), and ind...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-007-0808-2
更新日期:2008-01-01 00:00:00
abstract::Low persistence with osteoporosis medication is associated with higher fracture risk. Previous studies estimated that 1-year persistence with osteoporosis medication is low. Our aim was to study persistence with osteoporosis medication among patients with long-term follow-up (to 5 years). The InterAction Database (IAD...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-013-0440-2
更新日期:2013-09-01 00:00:00
abstract:INTRODUCTION:Very few studies have been performed to evaluate both the severity and site of aortic calcification (AC) in both end-stage kidney disease (ESKD) and diabetes mellitus (DM). The purpose of our study was to examine the utility of a newly developed three-dimensional (3D) visualization and quantification metho...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-020-01172-4
更新日期:2020-11-03 00:00:00
abstract::Osteoporosis has a strong genetic component, but the genes involved are poorly defined. Genome-wide scans in multiple populations have identified chromosome 1p36 as one region linked to bone mineral density (BMD). The tumor necrosis factor receptor 2 (TNFR2) at 1p36 is a positional and functional candidate gene in ost...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-005-0659-7
更新日期:2006-01-01 00:00:00
abstract::Fracture healing is a complex biological process involving the proliferation of mesenchymal progenitor cells, and chondrogenic, osteogenic, and angiogenic differentiation. The mechanisms underlying the proliferation and differentiation of mesenchymal progenitor cells remain unclear. Here, we demonstrate Dickkopf-relat...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-015-0711-1
更新日期:2016-11-01 00:00:00
abstract::Based on the relationship between bone strength, trabecular skeletal structure, and bone mineral density (BMD), structure parameters for assessing vertebral bone strength were studied using 18 cancellous bone blocks from the third lumbar vertebra of elderly persons. The trabecular bone pattern of each bone block was b...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s007740170035
更新日期:2001-01-01 00:00:00
abstract::Variation in drug response to hormone replacement therapy (HRT) may reflect genetic heterogeneity in the estrogen-related genes, possibly including estrogen receptor alpha (ERalpha) gene. However, only a few association studies of the drug response to HRT have been reported, focusing mainly on the intronic polymorphis...
journal_title:Journal of bone and mineral metabolism
pub_type: 杂志文章
doi:10.1007/s00774-005-0619-2
更新日期:2005-01-01 00:00:00