Development of new criteria for cortical bone histomorphometry in femoral neck: intra- and inter-observer reproducibility.


:Histomorphometry is commonly applied to study bone remodeling. Histological definitions of cortical bone boundaries have not been consistent. In this study, new criteria for specific definition of the transitional zone between the cortical and cancellous bone in the femoral neck were developed. The intra- and inter-observer reproducibility of this method was determined by quantitative histomorphometry and areal overlapping analysis. The undecalcified histological sections of femoral neck specimens (n = 6; from men aged 17-59 years) were processed and scanned to acquire histological images of complete bone sections. Specific criteria were applied to define histological boundaries. "Absolute cortex area" consisted of pure cortical bone tissue only, and was defined mainly based on the size of composite canals and their distance to an additional "guide" boundary (so-called "preliminary cortex boundary," the clear demarcation line of density between compact cortex and sparse trabeculae). Endocortical bone area was defined by recognizing characteristic endocortical structures adjacent to the preliminary cortical boundary. The present results suggested moderate to high reproducibility for low-magnification parameters (e.g., cortical bone area). The coefficient of variation (CV %) ranged from 0.02 to 5.61 in the intra-observer study and from 0.09 to 16.41 in the inter-observer study. However, the intra-observer reproducibility of some high-magnification parameters (e.g., osteoid perimeter/endocortical perimeter) was lower (CV %, 0.33-87.9). The overlapping of three histological areas in repeated analyses revealed highest intra- and inter-observer reproducibility for the absolute cortex area. This study provides specific criteria for the definition of histological boundaries for femoral neck bone specimens, which may aid more precise cortical bone histomorphometry.


J Bone Miner Metab


Tong XY,Malo M,Tamminen IS,Isaksson H,Jurvelin JS,Kröger H




Has Abstract


2015-01-01 00:00:00












  • Lactoferrin immuno-expression in human normal and neoplastic bone tissue.

    abstract::Lactoferrin (Lf) expression was investigated by using a Lf monoclonal antibody in 50 formalin-fixed and paraffin-embedded human bone tumours [10 giant cell tumours (GCTs), 7 osteoid osteomas, 6 ossifying fibromas, 19 enchondromas, 2 chondroblastomas, 2 chondrosarcomas, 2 chondroblastic osteosarcomas, 1 myeloma and 1 a...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Ieni A,Barresi V,Grosso M,Rosa MA,Tuccari G

    更新日期:2009-01-01 00:00:00

  • Can antiosteoporotic therapy reduce mortality in MRI-proved acute osteoporotic vertebral fractures?

    abstract::Patients with MRI-proved acute painful vertebral fractures in whom conservative pain management fails are frequently referred for vertebroplasty. This study investigated the effects of treating osteoporosis on the mortality rate of patients with MRI-proved acute osteoporosis-related vertebral fractures who had undergo...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Chen YC,Su FM,Cheng TT,Lin WC,Lui CC

    更新日期:2016-05-01 00:00:00

  • Activation of focal adhesion kinase induces extracellular signal-regulated kinase-mediated osteogenesis in tensile force-subjected periodontal ligament fibroblasts but not in osteoblasts.

    abstract::The exact mechanism by which focal adhesion kinase (FAK) translates mechanical signals into osteogenesis differentiation in force-subjected cells has not been elucidated. The responses to different forces differ according to the origin of cells and the type of stress applied. Therefore, the recruitment of osteoclast a...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Chen YJ,Shie MY,Hung CJ,Wu BC,Liu SL,Huang TH,Kao CT

    更新日期:2014-11-01 00:00:00

  • Impact of bisphosphonate wash-out prior to teriparatide therapy in clinical practice.

    abstract::Concurrent use of bisphosphonate therapy reduces the anabolic effect of teriparatide. Consequently, in clinical practice bisphosphonates are discontinued and teriparatide therapy held for a few months to allow bone turnover to increase. We aimed to evaluate the effect of prior bisphosphonate exposure and the effect of...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 临床试验,杂志文章


    authors: Keel C,Kraenzlin ME,Kraenzlin CA,Müller B,Meier C

    更新日期:2010-01-01 00:00:00

  • Production of IL-7 is increased in ovariectomized mice, but not RANKL mRNA expression by osteoblasts/stromal cells in bone, and IL-7 enhances generation of osteoclast precursors in vitro.

    abstract::Osteoclastogenic cytokines produced by T and B lineage cells and interleukin (IL)-7-induced expansion of the pool size of osteoclast precursors have been suggested to play an important role in acceleration of osteoclastogenesis induced by estrogen deficiency. However, the contribution of increased RANKL produced by os...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Sato T,Watanabe K,Masuhara M,Hada N,Hakeda Y

    更新日期:2007-01-01 00:00:00

  • Suppression of differentiation and proliferation of early chondrogenic cells by Notch.

    abstract::Notch is a transmembrane protein involved in cell fate determination. In the present study, we observed temporally and spatially restricted expression of Notch1 in developing cartilage. Notch1 was localized starting from the mesenchymal condensation stage of embryonic mouse forelimbs. Interestingly, although localizat...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Watanabe N,Tezuka Y,Matsuno K,Miyatani S,Morimura N,Yasuda M,Fujimaki R,Kuroda K,Hiraki Y,Hozumi N,Tezuka K

    更新日期:2003-01-01 00:00:00

  • Multiscale investigation on the effects of additional weight bearing in combination with low-magnitude high-frequency vibration on bone quality of growing female rats.

    abstract::This study aimed to explore the effects of additional weight bearing in combination with low-magnitude high-frequency vibration (LMHFV; 45 Hz, 0.3 g) on bone quality. One hundred twenty rats were randomly divided into ten groups; namely, sedentary (SED), additional weight bearing in which the rat wears a backpack whos...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Zhang T,Gao J,Fang J,Gong H

    更新日期:2018-03-01 00:00:00

  • Association of estrogen receptor alpha gene microsatellite polymorphism with annual changes in bone mineral density in Korean women with hormone replacement therapy.

    abstract::Variation in drug response to hormone replacement therapy (HRT) may reflect genetic heterogeneity in the estrogen-related genes, possibly including estrogen receptor alpha (ERalpha) gene. However, only a few association studies of the drug response to HRT have been reported, focusing mainly on the intronic polymorphis...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Yim CH,Choi JT,Choi HA,Kang YS,Moon IG,Yoon HK,Han IK,Kang DH,Han KO

    更新日期:2005-01-01 00:00:00

  • High calcium, phosphate and calcitriol supplementation leads to an osteocyte-like phenotype in calcified vessels and bone mineralisation defect in uremic rats.

    abstract::A link between vascular calcification and bone anomalies has been suggested in chronic kidney disease (CKD) patients with low bone turnover disease. We investigated the vascular expression of osteocyte markers in relation to bone microarchitecture and mineralization defects in a model of low bone turnover CKD rats wit...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Bisson SK,Ung RV,Picard S,Valade D,Agharazii M,Larivière R,Mac-Way F

    更新日期:2019-03-01 00:00:00

  • Treatment for patients with postmenopausal osteoporosis who have been placed on HRT and show a decrease in bone mineral density: effects of concomitant administration of vitamin K(2).

    abstract::We have been using hormone replacement therapy (HRT) as the treatment of choice for menopausal symptoms and osteoporosis. Estrogen increases bone mineral density (BMD) for 2 or 3 years, and only maintains BMD thereafter. In the present study, we investigated whether BMD improved with HRT in combination with vitamin K(...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Hidaka T,Hasegawa T,Fujimura M,Sakai M,Saito S

    更新日期:2002-01-01 00:00:00

  • Prevalence and involvement patterns of radiographic hand osteoarthritis in Japanese women: the Hizen-Oshima Study.

    abstract::The prevalence and pattern of joint involvement in radiographic hand osteoarthritis (OA) have been reported in Western populations, but similar data are lacking for Japanese. We examined this issue in 551 Japanese women aged > or = 40 years. Radiographs were obtained of both hands and graded according to the Kellgren-...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Toba N,Sakai A,Aoyagi K,Yoshida S,Honda S,Nakamura T

    更新日期:2006-01-01 00:00:00

  • Hindlimb-unloading suppresses B cell population in the bone marrow and peripheral circulation associated with OPN expression in circulating blood cells.

    abstract::Rodent hindlimb unloading (HU) by tail-suspension is a model to investigate disuse-induced bone loss in vivo. Previously, we have shown that osteopontin (OPN, also known as Spp1) is required for unloading-induced bone loss. However, how unloading affects OPN expression in the body is not fully understood. Here, we exa...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Ezura Y,Nagata J,Nagao M,Hemmi H,Hayata T,Rittling S,Denhardt DT,Noda M

    更新日期:2015-01-01 00:00:00

  • Effects of interleukin-11 on the expression of human bone sialoprotein gene.

    abstract::Interleukin-11 (IL-11) is a bone marrow stromal fibroblast-derived cytokine with a wide spectrum of activities in different biological systems. IL-11 and IL-6 are two cytokines known to rely on osteoblast-osteoclast communication for their effects on osteoclast differentiation. Bone sialoprotein (BSP) is a mineralized...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Matsumura H,Nakayama Y,Takai H,Ogata Y

    更新日期:2015-03-01 00:00:00

  • Enhancement of recombinant human bone morphogenetic protein-2 (rhBMP-2)-induced new bone formation by concurrent treatment with parathyroid hormone and a phosphodiesterase inhibitor, pentoxifylline.

    abstract::We investigated the enhancement of new bone |formation elicited ectopically by recombinant human bone morphogenetic protein-2 (rhBMP-2), using parathyroid hormone (PTH) and a phosphodiesterase inhibitor (PDEi), pentoxifylline (PTX), in an animal model. Collagen sponge sheet discs containing rhBMP were implanted onto t...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Horiuchi H,Saito N,Kinoshita T,Wakabayashi S,Tsutsumimoto T,Otsuru S,Takaoka K

    更新日期:2004-01-01 00:00:00

  • Biochemical bone turnover markers are useful tools to assess changes in bone metabolism in marmosets.

    abstract::This study was designed to investigate whether biochemical markers of bone resorption and formation could be determined in the serum and urine of marmosets (Callithrix jacchus), using standard laboratory chemistry methods and commercially available human kits. Consequently, the findings from this study will indicate w...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Angeliewa A,Budde M,Schlachter M,Hoyle NR,Bauss F

    更新日期:2004-01-01 00:00:00

  • Additive effects of glucosamine or risedronate for the treatment of osteoarthritis of the knee combined with home exercise: a prospective randomized 18-month trial.

    abstract::We have previously demonstrated the efficacy of therapeutic exercise for osteoarthritis (OA) of the knee. This study was performed to examine the additive effects of glucosamine or risedronate on the exercise therapy. In this study, 142 female patients with moderate OA of the knee, who had been recommended to undergo ...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章,随机对照试验


    authors: Kawasaki T,Kurosawa H,Ikeda H,Kim SG,Osawa A,Takazawa Y,Kubota M,Ishijima M

    更新日期:2008-01-01 00:00:00

  • Effects of genistein on vertebral trabecular bone microstructure, bone mineral density, microcracks, osteocyte density, and bone strength in ovariectomized rats.

    abstract::Until now, the effects of phytoestrogen on bone in both women and ovarian hormone-deficient animal models of osteoporosis have remained uncertain. We have aimed here to investigate the effect of genistein (GEN) on trabecular bone quality in ovariectomized (OVX) rats. Forty 7-month-old female Sprague-Dawley rats were r...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Dai R,Ma Y,Sheng Z,Jin Y,Zhang Y,Fang L,Fan H,Liao E

    更新日期:2008-01-01 00:00:00

  • Causes of secondary hyperparathyroidism in a healthy population: the Tromsø study.

    abstract::Secondary hyperparathyroidism (SHPT) develops as a compensatory mechanism when the body is in calcium deficit. SHPT may be harmful and has been associated with elevated blood pressure. The cause of SHPT could be low calcium intake, reduced intestinal calcium absorption, or increased excretion. However, the relative im...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Saleh F,Jorde R,Sundsfjord J,Haug E,Figenschau Y

    更新日期:2006-01-01 00:00:00

  • Cooperative effect of serum 25-hydroxyvitamin D concentration and a polymorphism of transforming growth factor-beta1 gene on the prevalence of vertebral fractures in postmenopausal osteoporosis.

    abstract::A T869-->C polymorphism of the transforming growth factor-beta1 (TGF-beta1) gene is reported to be associated with genetic susceptibility to both osteoporosis and vertebral fractures. A low serum 25-hydroxyvitamin D [25(OH)D] level is known to be associated with a higher risk for hip fracture. This study aimed to asse...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Mori S,Fuku N,Chiba Y,Tokimura F,Hosoi T,Kimbara Y,Tamura Y,Araki A,Tanaka M,Ito H

    更新日期:2010-07-01 00:00:00

  • Cloning and characterization of three PHEX homologues in Drosophila.

    abstract::Inactivating mutations and/or deletions of PHEX ( Phosphate-regulating gene with Homologies to Endopeptidase on the X chromosome) are responsible for X-linked hypophosphatemic rickets in humans. In the present study, three Drosophila PHEX homologues (dPHEX-1, -2, -3) were isolated by the screening of a Drosophila cDNA...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Ito M,Akai E,Izuka M,Segawa H,Kuwahata M,Miyamoto K

    更新日期:2004-01-01 00:00:00

  • Comparison of various biochemical measurements with bone mineral densitometry and quantitative ultrasound for the assessment of vertebral fracture.

    abstract::We have retrospectively studied postmenopausal elderly Japanese women (n = 288; age range, 60-75 years, 65.8 +/- 4.5 [mean +/- SD]) for the evaluation of biochemical measurements in assessment of bone mass and vertebral fracture, comparing with several bone mineral measurements and quantitative ultrasound (QUS) measur...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Matsushita R,Yamamoto I,Takada M,Hamanaka Y,Yuh I,Morita R

    更新日期:2000-01-01 00:00:00

  • Zoledronic acid administration failed to prevent bone loss at the knee in persons with acute spinal cord injury: an observational cohort study.

    abstract::After acute spinal cord injury (SCI), rapid depletion of the sublesional skeleton occurs, particularly at the distal femur and proximal tibia. Subsequently, fragility fractures of the knee may occur. We determined the efficacy of zoledronic acid to prevent sublesional bone mineral density (BMD) loss at 6 and 12 months...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Bauman WA,Cirnigliaro CM,La Fountaine MF,Martinez L,Kirshblum SC,Spungen AM

    更新日期:2015-07-01 00:00:00

  • The effects of α-zearalanol on the proliferation of bone-marrow-derived mesenchymal stem cells and their differentiation into osteoblasts.

    abstract::The aim of this study was to explore the effects of α-zearalanol (α-ZAL) on the proliferation of mouse bone-marrow-derived mesenchymal stem cells (BMSCs) and their differentiation into osteoblasts. Six- to eight-week-old BALB/C mice were used either as recipients or as bone marrow donors. BMSCs were isolated and colle...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Zong S,Zeng G,Fang Y,Peng J,Zou B,Gao T,Zhao J

    更新日期:2016-03-01 00:00:00

  • Incidence of osteonecrosis of the jaw in Japanese osteoporosis patients taking minodronic acid.

    abstract::Osteonecrosis of the jaw (ONJ) associated with bisphosphonate therapy is a rare but severe side effect in osteoporosis patients. Recently, the number of osteoporosis patients with ONJ has dramatically increased in Japan. This has contributed to an increase in the number of patients avoiding extractions. However, there...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Taguchi A,Uemura Y,Imai T,Tanaka S,Ohta H,Nakamura T,Orimo H,Sugimoto T,Soen S,Shiraki M,Adequate Treatment of Osteoporosis (A-TOP) research group.

    更新日期:2019-09-01 00:00:00

  • Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa.

    abstract::A cell line of murine osteogenic progenitor cells, Kusa, was established from femoral bone marrow stromal cells with other types of mesenchymal progenitor cells. We characterized two sublines of Kusa (Kusa-A1 and Kusa-O) from several aspects, including the use of an expression profiling system, a cDNA microarray. The ...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Kawashima N,Shindo K,Sakamoto K,Kondo H,Umezawa A,Kasugai S,Perbal B,Suda H,Takagi M,Katsube K

    更新日期:2005-01-01 00:00:00

  • Effects of sciatic neurectomy on the femur in growing rats: application of peripheral quantitative computed tomography and Fourier transform infrared spectroscopy.

    abstract::The effects of unilateral sciatic neurectomy (USN) on the development of the femur were studied in 15 growing Wistar-derived rats (age, 5 weeks). The rats were divided into four groups: USN-operated group (right femur), USN-nonoperated group (left femur), sham-operated group (right femur), and sham-nonoperated group (...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Yonezu H,Ikata T,Takata S,Shibata A

    更新日期:1999-01-01 00:00:00

  • Diagnostic criteria for primary osteoporosis: year 2012 revision.

    abstract::In 1995, the Japanese Society for Bone and Mineral Metabolism (now the Japanese Society for Bone and Mineral Research) established the Osteoporosis Diagnostic Criteria Review Committee. Following discussion held at the 13th scientific meeting of the Society in 1996, the Committee, with the consensus of its members, pr...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Soen S,Fukunaga M,Sugimoto T,Sone T,Fujiwara S,Endo N,Gorai I,Shiraki M,Hagino H,Hosoi T,Ohta H,Yoneda T,Tomomitsu T,Japanese Society for Bone and Mineral Research and Japan Osteoporosis Society Joint Review Committee for the R

    更新日期:2013-05-01 00:00:00

  • The visualization and evaluation of bone architecture in the rat using three-dimensional X-ray microcomputed tomography.

    abstract::Microcomputed tomography allows the true three-dimensional structure of bone to be assessed by a nondestructive analysis. This article describes how this technique has for the first time been applied to rat bone to determine the effects of aging, ovariectomy, and antiresorptive drugs on bone structure and how these re...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Barbier A,Martel C,de Vernejoul MC,Tirode F,Nys M,Mocaer G,Morieux C,Murakami H,Lacheretz F

    更新日期:1999-01-01 00:00:00

  • A fourier transform infrared spectroscopic and solid-state NMR study of bone mineral in osteogenesis imperfecta.

    abstract::Fourier transform infrared spectroscopy and 31P solid-state nuclear magnetic resonance spectroscopy were used to determine if any structural or compositional differences in osteogenesis imperfecta (OI) bone mineral could be detected that might help to explain the bone fragility observed in this disease. A previous stu...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Cassella JP,Barrie PJ,Garrington N,Ali SY

    更新日期:2000-01-01 00:00:00

  • Simvastatin induces estrogen receptor-alpha expression in bone, restores bone loss, and decreases ERα expression and uterine wet weight in ovariectomized rats.

    abstract::We previously reported that simvastatin induces estrogen receptor-alpha (ERα) in murine bone marrow stromal cells in vitro. In this study, we investigated the effect of simvastatin on ERα expression in bone and uterus in ovariectomized (OVX) rats and evaluated bone mass, bone strength, and uterine wet weight. Three-mo...

    journal_title:Journal of bone and mineral metabolism

    pub_type: 杂志文章


    authors: Li X,Song QS,Wang JY,Leng HJ,Chen ZQ,Liu ZJ,Dang GT,Song CL

    更新日期:2011-07-01 00:00:00