Abstract:
:A single-tube multiplex assay of a small set of ancestry-informative markers (AIMs) for effectively estimating individual ancestry and admixture is an ideal forensic tool to trace the population origin of an unknown DNA sample. We present a newly developed 27-plex single nucleotide polymorphism (SNP) panel with highly robust and balanced differential power to perfectly assign individuals to African, European, and East Asian ancestries. Evaluating 968 previously described intercontinental AIMs from three HapMap population genotyping datasets (Yoruban in Ibadan, Nigeria (YRI); Utah residents with Northern and Western European ancestry from the Centre de'Etude du Polymorphism Humain (CEPH) collection (CEU); and Han Chinese in Beijing, China (CHB)), the best set of markers was selected on the basis of Hardy-Weinberg equilibrium (p > 0.00001), population-specific allele frequency (two of three δ values >0.5), according to linkage disequilibrium (r (2) < 0.2), and capable of being multiplexed in one tube and detected by capillary electrophoresis. The 27-SNP panel was first validated by assigning the ancestry of the 11 populations in the HapMap project. Then, we tested the 27-plex SNP assay with 1164 individuals from 17 additional populations. The results demonstrated that the SNP panel was successful for ancestry inference of individuals with African, European, and East Asian ancestry. Furthermore, the system performed well when inferring the admixture of Eurasians (EUR/EAS) after analyzing admixed populations from Xinjiang (Central Asian) as follows: Tajik (68:27), Uyghur (49:46), Kirgiz (40:57), and Kazak (36:60). For individual analyses, we interpreted each sample with a three-ancestry component percentage and a population match probability sequence. This multiplex assay is a convenient and cost-effective tool to assist in criminal investigations, as well as to correct for the effects of population stratification for case-control studies.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Wei YL,Wei L,Zhao L,Sun QF,Jiang L,Zhang T,Liu HB,Chen JG,Ye J,Hu L,Li CXdoi
10.1007/s00414-015-1183-5subject
Has Abstractpub_date
2016-01-01 00:00:00pages
27-37issue
1eissn
0937-9827issn
1437-1596pii
10.1007/s00414-015-1183-5journal_volume
130pub_type
杂志文章abstract::Forensic age estimation of living individuals has become increasingly important in forensic practice with the constant increase of migration movements to developed countries. The method of Greulich and Pyle is one of the most frequently used methods for age determination. The aim of our study was to verify the reliabi...
journal_title:International journal of legal medicine
pub_type: 杂志文章
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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abstract::When blood reaches an extracorporeal surface, a drying process is initiated. Properties of this drying process may be crucial for the correct assessment of case-specific time lapses, however, there is a lack of systematic studies concerning the drying times of blood. We present a study on drying properties of small bl...
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journal_title:International journal of legal medicine
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doi:10.1007/s00414-012-0811-6
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journal_title:International journal of legal medicine
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pub_type: 杂志文章
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
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journal_title:International journal of legal medicine
pub_type: 杂志文章
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