Lippia alba essential oil promotes survival of silver catfish (Rhamdia quelen) infected with Aeromonas sp.

Abstract:

:In vitro and in vivo activity of the Lippia alba essential oil (EO) against Aeromonas sp. was evaluated. In the in vitro assay the minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of EO for Aeromonas cells were determined using the microdilution method. Twenty five strains of Aeromonas sp. isolated from infected fish obtained from local fish farms were used. MIC and MBC values were 2862 and 5998 µg mL-1 for L. alba EO and 0.5 and 1.2 µg mL-1 for gentamicin, respectively. In the in vivo assay silver catfish juveniles (Rhamdia quelen) (7.50 ± 1.85 g and 10.0 ± 1.0 cm) with typical injuries associated to Aeromonas infection were divided into four treatments (in triplicate n=10): untreated fish (negative control), 10 mg L-1 of gentamicin, and 20 or 50 µL L-1 of EO. Fish were maintained in aerated 20 L plastic boxes. After 10 days survival of silver catfish infected with Aermonas sp. and treated with essential oil (50 µL L-1) was greater than 90%.

journal_name

An Acad Bras Cienc

authors

Sutili FJ,Cunha MA,Ziech RE,Krewer CC,Zeppenfeld CC,Heldwein CG,Gressler LT,Heinzmann BM,Vargas AC,Baldisserotto B

doi

10.1590/0001-3765201520130442

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

95-100

issue

1

eissn

0001-3765

issn

1678-2690

pii

S0001-37652015005030442

journal_volume

87

pub_type

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