Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples.

Abstract:

:The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.

journal_name

An Acad Bras Cienc

authors

Pie MR,Ströher PR,Agostinis AO,Belmonte-Lopes R,Tadra-Sfeir MZ,Ostrensky A

doi

10.1590/0001-3765201720160723

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

1041-1045

issue

2

eissn

0001-3765

issn

1678-2690

pii

S0001-37652017000301041

journal_volume

89

pub_type

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