Abstract:
:Pathogenic microbial detection and control in laboratory animal facilities is essential to guarantee animal welfare, data validity and reproducibility. Helicobacter spp. are known to affect mice health, what may interfere with experimental outcomes. This study aimed to screen for Helicobacter spp. in mice from animal facilities in Rio de Janeiro, Brazil using a PCR-based method. Primers designed to specifically identify Helicobacter spp. were used to amplify feces or intestine DNA extracted of mice from four different animal facilities. The expected 375 base pairs (bp) amplicon was purified, sequenced and a similarity of 95% was observed when compared to deposited sequences of H. hepaticus and H. bilis. In our screening, Helicobacter spp. was detected in ~59% of fecal and ~70% of intestine samples. Our study is the first to screen for Helicobacter spp. in mouse facilities of a Rio de Janeiro University using a low cost, rapid molecular diagnostic test. Although Helicobacter spp. screening is not mandatory according to Brazilian animal welfare regulation it is recommended by institutional animal health monitoring programs guidelines worldwide, including ARRIVE, AAALAC and FELASA.
journal_name
An Acad Bras Ciencjournal_title
Anais da Academia Brasileira de Cienciasauthors
Matos-Rodrigues GE,Masseron CC,Silva FJMD,Frajblat M,Moreira LO,Martins RAPdoi
10.1590/0001-3765202020191517subject
Has Abstractpub_date
2020-01-01 00:00:00pages
e20191517issue
4eissn
0001-3765issn
1678-2690pii
S0001-37652020000700801journal_volume
92pub_type
杂志文章abstract::Black Mulberry (Morus nigra L.) belongs to Moraceae family. The present study evaluated the possible genotoxic and/or protective activities of black mulberry fruit juice (BMFJ), in vitro, using mitomycin C (MMC) as a positive control, by chromosomal aberrations and micronucleus assays. Human lymphocytes were treated w...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920190337
更新日期:2019-10-28 00:00:00
abstract::In the Budgerigar Melopsittacus undulatus, the significance of a conspecific alarm call was investigated in two seasons, winter and spring. Two qualitatively different behavioral responses were displayed by the receivers in reply to playbacks: call(s) and/or taking flight(s). The comparative analysis of the number of ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652004000200025
更新日期:2004-06-01 00:00:00
abstract::Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproli...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720160725
更新日期:2017-01-01 00:00:00
abstract::Japanese Islands are covered with weathered volcanic rocks and soils. Terraced rice field are located in green-tuff areas which are very fertile but where landslides occur associated to strong earthquakes. The Xray diffraction and X-ray fluorescence analyses of the soils in landslide area identified predominant smecti...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652006000400010
更新日期:2006-12-01 00:00:00
abstract::This study investigated the physiological effects of a pesticide based on Bacillus thuringiensis (Dipel-WP®) added in the water and diet of Piaractus mesopotamicus during 24 and 48 h. It was added 0.13 g of de B. thuringiensis per kg of commercial feed; and for the fish subjected to the biopesticide in the water of th...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920180474
更新日期:2019-06-19 00:00:00
abstract::In the present study we analyzed morphological and metabolic alterations in dams nursing small litters and their consequences to offspring throughout lactation. Offspring sizes were adjusted to Small Litter (SL, 3 pups/ dam) and Normal Litter (NL, 9 pups/ dam). Body weight, food intake, white adipose tissue (WAT) cont...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920170971
更新日期:2019-03-21 00:00:00
abstract::A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and s...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652003000100007
更新日期:2003-03-01 00:00:00
abstract::Bioceramic composites were obtained from chitosan and hydroxyapatite pastes synthesized at physiological temperature according to two different syntheses approaches. Usual analytical techniques (X-ray diffraction analysis, Fourier transformed infrared spectroscopy, Thermo gravimetric analysis, Scanning electron micros...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652001000400006
更新日期:2001-12-01 00:00:00
abstract::Observations on the ecology of silvatic Triatominae are still fragmentary, principally because target species in control process are almost exclusively domestic. Report of silvatic species invading human dwellings must lead research to be focused on their original ecology, in order to assist in understanding their pro...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652005000300006
更新日期:2005-09-01 00:00:00
abstract::The purpose of this study was to analyze the seasonal variation in the nutrient and pigment content of leaves from five tree species - of which three are perennial (Cupania vernalis, Matayba elaeagnoides and Nectandra lanceolata) and two are deciduous (Cedrela fissilis and Jacaranda micrantha) - in an ecotone between ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620140605
更新日期:2016-01-01 00:00:00
abstract::The aim of this study was to evaluate potential DNA damage and cytotoxicity in pathology laboratory technicians exposed to organic solvents, mainly xylene. Peripheral blood and buccal cells samples were collected from 18 technicians occupationally exposed to organic solvents and 11 non-exposed individuals. The technic...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620150194
更新日期:2016-03-01 00:00:00
abstract::We propose a new four-parameter lifetime model, called the extended log-logistic distribution, to generalize the two-parameter log-logistic model. The new model is quite flexible to analyze positive data. We provide some mathematical properties including explicit expressions for the ordinary and incomplete moments, pr...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720150579
更新日期:2017-01-01 00:00:00
abstract::The characteristic of immersion, involvement and motivation have made serious games an important tool to be used in the medical field. However, there are people that, having some sort of physical disability, are not able to, or do not feel motivated to play. This work presents a strategy to support human- computer int...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020190273
更新日期:2020-02-17 00:00:00
abstract::The adsorption of Violet Remazol 5R (VR 5) on wood sawdust modified with succinic anhydride (SSA) as a function of contact time, pH, and initial dye concentrations was investigated using a batch technique under ambient conditions. The SSA obtained was confirmed by IR spectroscopy, thermogravimetry and 13C NMR, and deg...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620140656
更新日期:2016-09-01 00:00:00
abstract::The objective of this study was to estimate variance components for performance and carcass traits in a paternal broiler line. The (co)variance components were estimated by the restricted maximum likelihood method applied to the animal model, including the fixed effect of group (sex and hatch) and additive genetic and...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020180697
更新日期:2020-01-01 00:00:00
abstract::Drought is a natural and recurrent phenomenon. It is considered 'a natural disaster' whenever it occurs in an intensive manner in highly populated regions, resulting in significant damage (material and human) and loss (socioeconomic). This paper presents the efforts developed to monitor the impact of drought in the se...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720170209
更新日期:2019-01-01 00:00:00
abstract::Acute intermittent porphyria is the most common acute porphyria caused by a decrease in hepatic porphobilinogen deaminase activity, resulting in an accumulation of delta-aminolevulinic acid and porphobilinogen. This disease shows nonspecific signs and symptoms that can be confused with other diseases, thereby making t...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/S0001-37652013000300019
更新日期:2013-09-01 00:00:00
abstract::In the province of Misiones (Argentina), the filling of the Yacyretá Reservoir (Argentina-Paraguay) to its final dimensions in 2011 formed new aquatic ecosystem, e.g., Garupá Stream was converted into a subreservoir. Reports have been made in this stream of adult individuals and spawning of the Family Sciaenidae, exce...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020180783
更新日期:2020-01-01 00:00:00
abstract::Piper aduncum found naturally in the Amazon and southeastern Brazil, is known for its secondary metabolites that have activity on insects. Anticarsia gemmatalis and Spodoptera frugiperda are among the major insect pests associated with agricultural production. This research evaluated the biological activity of hexane,...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720170194
更新日期:2017-07-01 00:00:00
abstract::Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spo...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820170250
更新日期:2018-01-01 00:00:00
abstract::Microglial cells within the developing central nervous system (CNS) originate from mesodermic precursors of hematopoietic lineage that enter the nervous parenchyma from the meninges, ventricular space and/or blood stream. Once in the nervous parenchyma, microglial cells increase in number and disperse throughout the C...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章,评审
doi:10.1590/s0001-37652000000100013
更新日期:2000-01-01 00:00:00
abstract::Multidrug resistance to chemotherapy is a major obstacle in the treatment of cancer patients. The best characterised mechanism responsible for multidrug resistance involves the expression of the MDR-1 gene product, P-glycoprotein. However, the resistance process is multifactorial. Studies of multidrug resistance mecha...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章,评审
doi:10.1590/s0001-37652001000100007
更新日期:2001-03-01 00:00:00
abstract::This paper presents a sedimentological and stratigraphical study of Quaternary (Middle to Late Pleistocene/Holocene) continental carbonates outcrops inside Pantanal Basin and its surroundings, especially in Serra da Bodoquena, Pantanal do Miranda and Corumbá/Ladário plateau, in the state of Mato Grosso do Sul, as well...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720160584
更新日期:2017-05-01 00:00:00
abstract::The aim of this work was to investigate the phenolic content and antioxidant capacity of infusions of commercial herb samples (fennel, anise, peppermint, lemon grass and lemon balm) popularly consumed in Brazil. The infusion preparation for phenolic extraction was optimized using multivariate planning. Spectrophotomet...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020190646
更新日期:2020-11-11 00:00:00
abstract::Studies on belowground roots biomass have increasingly reported the importance of the contribution of this compartment in carbon stock maintenance in mangrove forests. To date, there are no estimates of this contribution in Brazilian mangrove forests, although the country has the second largest area of mangroves world...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720160496
更新日期:2017-07-01 00:00:00
abstract::Pollen grain morphology of Bromeliaceae species collected in areas of the Atlantic Rainforest of southeastern Brazil was studied. The following species were analyzed: Aechmea bambusoides L.B.Sm. & Reitz, A. nudicaulis (L.) Griseb., A. ramosa Mart. ex Schult.f., Ananas bracteatus (Lindl.) Schult.f., Billbergia distachi...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620140715
更新日期:2016-01-01 00:00:00
abstract::Urotrema scabridum Braun 1900 and Urotrema macrotestis Mané-Garzón and Telias 1965 are reported from the small intestine of Eumops glaucinus (Wagner, 1843). The species were differentiated by the body width, the size and position of acetabulum, the size of testis, the caecal termination, and the distribution of vitell...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820180648
更新日期:2019-03-21 00:00:00
abstract::The use of sensors and optical fibers for monitoring structures is continuously growing. Strains, displacements, accelerations, temperatures are just a few of the monitoring possibilities. In the case of dam structures, concrete face rockfill dams constitute a type of structures where the monitoring of water infiltrat...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820170144
更新日期:2018-04-01 00:00:00
abstract::The objective of this work was to study the reduction in the capsaicin toxicity by encapsulation in liposomes. Capsaicin was extracted from peppers and characterized with high performance liquid chromatography (HPLC). We determined the zeta potential, polydispersivity index (PdI) and vesicle size of liposomes. Wistar ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620150309
更新日期:2016-09-01 00:00:00
abstract::We investigated reproductive features of the dipsadid snake Erythrolamprus poecilogyrus sublineatus in the southernmost Brazilian coast, a subtropical region characterized by well-marked seasons. Females are significantly smaller than males, have a shorter tail, and reach sexual maturity at later times along their dev...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720160805
更新日期:2017-01-01 00:00:00