PCR-based detection of Helicobacter spp. in animal facilities of a University in Rio de Janeiro, Brazil.

Abstract:

:Pathogenic microbial detection and control in laboratory animal facilities is essential to guarantee animal welfare, data validity and reproducibility. Helicobacter spp. are known to affect mice health, what may interfere with experimental outcomes. This study aimed to screen for Helicobacter spp. in mice from animal facilities in Rio de Janeiro, Brazil using a PCR-based method. Primers designed to specifically identify Helicobacter spp. were used to amplify feces or intestine DNA extracted of mice from four different animal facilities. The expected 375 base pairs (bp) amplicon was purified, sequenced and a similarity of 95% was observed when compared to deposited sequences of H. hepaticus and H. bilis. In our screening, Helicobacter spp. was detected in ~59% of fecal and ~70% of intestine samples. Our study is the first to screen for Helicobacter spp. in mouse facilities of a Rio de Janeiro University using a low cost, rapid molecular diagnostic test. Although Helicobacter spp. screening is not mandatory according to Brazilian animal welfare regulation it is recommended by institutional animal health monitoring programs guidelines worldwide, including ARRIVE, AAALAC and FELASA.

journal_name

An Acad Bras Cienc

authors

Matos-Rodrigues GE,Masseron CC,Silva FJMD,Frajblat M,Moreira LO,Martins RAP

doi

10.1590/0001-3765202020191517

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

e20191517

issue

4

eissn

0001-3765

issn

1678-2690

pii

S0001-37652020000700801

journal_volume

92

pub_type

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