Abstract:
:Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.
journal_name
An Acad Bras Ciencjournal_title
Anais da Academia Brasileira de Cienciasauthors
Belfort-Neto R,Nussenblatt V,Rizzo L,Muccioli C,Silveira C,Nussenblatt R,Khan A,Sibley LD,Belfort R Jrdoi
10.1590/s0001-37652007000100013subject
Has Abstractpub_date
2007-03-01 00:00:00pages
111-4issue
1eissn
0001-3765issn
1678-2690pii
S0001-37652007000100013journal_volume
79pub_type
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