Abstract:
:Properties of L(+) lactate dehydrogenase (LDH) of Electrophorus electricus (L.) electric organ were studied, comparing the substrates pyruvate and lactate. Electric organ LDH is a soluble enzyme with a pH optimum of 7.4 for pyruvate and 9.0 for lactate. The apparent Km was lower for pyruvate (Km = 2.5 X 10(-4) M) than for lactate (Km = 1.5 X 10(-2) M). With lactate as a substrate at pH 7.4, malonate, oxalate and pyruvate inhibited competitively. For pyruvate as substrate at pH 9.0 malonate inhibited non-competitively and oxalate shiwed uncompetitive inhibition. The different effects of the carboxylic acids on LDH activity suggest different stereospecificities of the two enzyme-coenzyme complexes in the forward and reserve reactions. The reactions of electric organ LDH with substrates and inhibitors are consistent with electrophoretic analysis suggesting that the enzyme is of the M-type.
journal_name
An Acad Bras Ciencjournal_title
Anais da Academia Brasileira de Cienciasauthors
Torres-da Matta J,Nery da Matta A,Hassón-Voloch Asubject
Has Abstractpub_date
1976-01-01 00:00:00pages
145-51issue
1eissn
0001-3765issn
1678-2690journal_volume
48pub_type
杂志文章abstract::In the present work, we report the synthesis and characterization of five (4-alkylalmino-4'-fluoro)-diphenyl sulphones, four of which have not been described before. In a preliminary evaluation of their biological activity, these products showed no effect on several Gram-positive and Gram-negative bacteria and yeasts....
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::The objective of this study was to evaluate the effect of controlled atmosphere (CA) on quality preservation of 'Laetitia' plums, mainly on internal breakdown, in order to determine the best CA storage conditions. Two experiments were carried out one in 2010, and another in 2011. In 2010, besides cold storage (CS; 21....
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201420130237
更新日期:2014-03-01 00:00:00
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652010000400028
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abstract::Fruits are rich in minerals, which are essential for a wide variety of metabolic and physiologic processes in the human body. The use of frozen fruits has greatly spread in the last years not only in the preparation of juices, but also as raw material for yogurts, candies, cookies, cakes, ice creams, and children's fo...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652010000400008
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abstract::Agromyza Fallén (Diptera, Agromyzidae) is a genus of leaf mining flies, including species with high economic importance. The knowledge of this genus is very poor in the neotropics, with 12 known species, only six of them recorded from Brazil. This paper describes two new Agromyza species from "Cerrado" and "Pantanal" ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620160210
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720160519
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820170871
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract::In the present study we analyzed morphological and metabolic alterations in dams nursing small litters and their consequences to offspring throughout lactation. Offspring sizes were adjusted to Small Litter (SL, 3 pups/ dam) and Normal Litter (NL, 9 pups/ dam). Body weight, food intake, white adipose tissue (WAT) cont...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920170971
更新日期:2019-03-21 00:00:00
abstract::The enigmatic genus Stenocrates Burmeister has 52 species that are widely distributed from Mexico to Argentina, but mainly in South America. In the present study, the larva of the third instar and pupa of Stenocrates agricola Dechambre & Hardy, 2004 are described and illustrated based on specimens from Argentina, this...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020190967
更新日期:2020-10-23 00:00:00
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620150228
更新日期:2016-01-01 00:00:00
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920180286
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020181120
更新日期:2020-04-17 00:00:00
abstract::Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes necessitates deeper knowledg...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020180477
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abstract::Drosophila serido and D. antonietae are sibling species belonging to the Drosophila buzzatii cluster. Morphologically, they can only be discriminated by quantitative traits. In this paper we analyze the length and equalized average curvature of four regions of the aedeagus of D. antonietae and D. serido. Specimens of ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652006000200002
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820170733
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abstract::Cyperaceae is an angiosperm family with the greatest diversity of species with Kranz anatomy. Four different types of Kranz anatomy (chlorocyperoid, eleocharoid, fimbristyloid and rhynchosporoid) have been described for this angiosperm family, and the occurrence and structural characteristics of these types are import...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201520150175
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abstract::Over the past two decades, the record of South American unenlagiine dromaeosaurids was substantially increased both in quantity as well as in quality of specimens. Here is presented a summary review of the South American record for these theropods.Unenlagia comahuensis, Unenlagia paynemili, and Neuquenraptor argentinu...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章,评审
doi:10.1590/s0001-37652011000100009
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abstract::The tritheledontid Riograndia guaibensis was the first cynodont described for the "Caturrita Formation" fauna from the Late Triassic of southern Brazil (Santa Maria 2 Sequence). The type materials did not preserve anatomical information regarding braincase, occiput, basicranium, zygomatic arch, postdentary bones and c...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652011000100021
更新日期:2011-03-01 00:00:00
abstract::Many essential oils (EOs) of different plant species possess interesting antimicrobial effects on buccal bacteria and antioxidant properties. Eugenia klotzschiana Berg (pêra-do-cerrado, in Portuguese) is a species of Myrtaceae with restricted distribution in the Cerrado. The essential oils were extracted through the h...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720160652
更新日期:2017-07-01 00:00:00
abstract::This paper presents a sedimentological and stratigraphical study of Quaternary (Middle to Late Pleistocene/Holocene) continental carbonates outcrops inside Pantanal Basin and its surroundings, especially in Serra da Bodoquena, Pantanal do Miranda and Corumbá/Ladário plateau, in the state of Mato Grosso do Sul, as well...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720160584
更新日期:2017-05-01 00:00:00
abstract::Two major memory systems have been recognized over the years (Squire 1987): the declarative memory system, which is under the control of the hippocampus and related temporal lobe structures, and the procedural or habit memory system, which is under the control of the striatum and its connections. Most if not all learn...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章,评审
doi:10.1590/s0001-37652006000300011
更新日期:2006-09-01 00:00:00
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journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720170240
更新日期:2017-01-01 00:00:00
abstract::The aquatic environment presents daily and/or seasonal variations in dissolved oxygen (DO) levels. Piava faces different DO levels in the water due to its distributional characteristics. The goal of this study was to describe the effects of low DO levels on plasma ion, biochemical and oxidative variables in piava juve...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920180395
更新日期:2019-08-19 00:00:00
abstract::Programs of Science and Technology research have grown significantly in Brazil over the last decades. Until the 1980s the so-called undirected programs, without specific goals and requiring only scientific merit, prevailed. The few programs with defined goals in this period were never objectively assessed. The same si...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652007000300014
更新日期:2007-09-01 00:00:00
abstract::The present study was designed to investigate the ameliorative potential of Ocimum sanctum and its saponin rich fraction in chronic constriction injury-induced neuropathic pain in rats. The chronic constriction injury was induced by placing four loose ligatures around the sciatic nerve, proximal to its trifurcation. T...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201520130008
更新日期:2015-03-01 00:00:00
abstract::This paper records the first occurrence of the genus Myrmicium Westwood, 1854 in the Cretaceous of Gondwana and describes it as a new species Myrmicium araripterum sp. nov, based on the most complete specimen of this genus yet known, which represents the largest specimen of the grade "Symphyta" ever found in the Crato...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020200479
更新日期:2020-01-01 00:00:00
abstract::Two-year trials (20062007) suggested that the use of calcium oxide (CaO) on acid soils increased soil pH and yields in strawberry cultivars Marmolada, Selena and Senga Sengana, under the environmental conditions of Cacak (Western Serbia). The highest yield was obtained when CaO was applied at 750 kg ha-1 rate. Further...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652009000200012
更新日期:2009-06-01 00:00:00