Ultra-low temperature conservation of Brazilian pine embryogenic cultures.

Abstract:

:This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.

journal_name

An Acad Bras Cienc

authors

Demarchi G,Stefenon VM,Steiner N,Vieira FN,Dal Vesco LL,Guerra MP

doi

10.1590/0001-3765201420130405

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

2057-64

issue

4

eissn

0001-3765

issn

1678-2690

pii

S0001-37652014000402057

journal_volume

86

pub_type

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