Abstract:
:This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.
journal_name
An Acad Bras Ciencjournal_title
Anais da Academia Brasileira de Cienciasauthors
Demarchi G,Stefenon VM,Steiner N,Vieira FN,Dal Vesco LL,Guerra MPdoi
10.1590/0001-3765201420130405subject
Has Abstractpub_date
2014-12-01 00:00:00pages
2057-64issue
4eissn
0001-3765issn
1678-2690pii
S0001-37652014000402057journal_volume
86pub_type
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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journal_title:Anais da Academia Brasileira de Ciencias
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