Abstract:
:The infrared spectrum (amide I' region) of Kunitz soybean trypsin inhibitor (SBTI) was obtained in D2O solution and resolved into Gaussian components. A prominent broad band centered at 1643 cm-1 is shown on the unresolved spectrum, which is usually assigned to N-deuterated peptide groups in an unordered structure, since SBTI is known to be devoid of alpha-helix by CD and X-ray crystallographic studies. In addition, shoulders are evident at 1632 cm-1 and 1676 cm-1, which correspond probably to the v(pi, O) and v(O, pi) components assigned to an antiparallel-chain beta-pleated sheet structure. Parameters (maximum absorptivity, wavenumber at the maximum of the band, and half-width of the band at half-height) for the four Gaussian component bands (in which the amide I' band was resolved) are given. A crude estimation of 4% is obtained for antiparallel beta-sheet in SBTI, i.e., this protein would be practically devoid of such a beta-structure. Notwithstanding the fact that this result is apparently in agreement with the far-UV CD spectrum (data reported in the literature), the predominant conformation class found in SBTI has been demonstrated to be approximate beta-sheet structures, with a small amount of regular sheet (Sweet et al., (1974) Biochemistry 13: 4212-4228).
journal_name
An Acad Bras Ciencjournal_title
Anais da Academia Brasileira de Cienciasauthors
Ventura MMsubject
Has Abstractpub_date
1989-01-01 00:00:00pages
373-7issue
3eissn
0001-3765issn
1678-2690journal_volume
61pub_type
杂志文章abstract::Epigeic coleopteran species are linked by complex trophic webs and implicated in several ecosystem services for agriculture. Although there are reports of ground-dwelling Coleopterans inhabiting main extensive agroecosystems, their assemblages in horticultural crops in Argentina have been little explored. We examined ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020181391
更新日期:2020-01-01 00:00:00
abstract::Unlike the majority of the nudibranchs, Glaucidae lives adrift at the sea surface within Tropical and Subtropical ocean basins, feeding on cnidarians or using them to attach their egg strings as a reproductive strategy. The latitudinal distribution of Glaucus atlanticus throughout the Brazilian Province is influenced ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920180154
更新日期:2019-01-01 00:00:00
abstract::Twenty-two nitrogen-fixing Bacillus azotofixans strains were shown to produce an inhibition zone against themselves in plate assays. The B. azotofixans type strain P3L-5, chosen for further studies, produced inhibition zones against various Bacillus strains and other bacterial genera. This antibacterial substance was ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract::The Bahía Blanca estuary (Argentina) has a morphological configuration resulting from hydrological and sedimentary processes related to Late Quaternary sea level changes. This estuarine system occupies a large coastal plain with a dense net of tidal channels, low-altitude islands and large intertidal flats. Little is ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract::The objective of this study was to investigate the effects of 2% L-glutamine supplementation on myenteric innervation in the ileum of diabetic rats, grouped as follows: normoglycemic (N); normoglycemic supplemented with L-glutamine (NG); diabetic (D); and diabetic supplemented with L-glutamine (DG). The ileums were su...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620150228
更新日期:2016-01-01 00:00:00
abstract::The purpose of this study was to assess the effect of different carbohydrate and lipid (CHO/L) ratios on the performance and energy metabolism of hybrid catfish (♂Pseudoplatystoma corruscans × ♀ Pseudoplatystoma fasciatum). One hundred and sixty-eight fish (104.9±16.5 g BW) were distributed into 24 100-L tanks. The ex...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920190075
更新日期:2019-11-25 00:00:00
abstract::A soil isolate, Penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-PG). By optimizing growth conditions, P. janthinellum sw09 produced high amount of exo-PG (16.54 units/mL). The crude enzyme was purified by...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620150051
更新日期:2016-01-01 00:00:00
abstract::The adsorption of Violet Remazol 5R (VR 5) on wood sawdust modified with succinic anhydride (SSA) as a function of contact time, pH, and initial dye concentrations was investigated using a batch technique under ambient conditions. The SSA obtained was confirmed by IR spectroscopy, thermogravimetry and 13C NMR, and deg...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620140656
更新日期:2016-09-01 00:00:00
abstract::The Epinephelidae form a group of species of high biological and economical interests. It´s phylogeographic patterns are not well known especially the distributed populations in the western region of the Atlantic Ocean. Among the representatives is a small species called Cephalopholis fulva, Coney, which presents a wi...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201520130411
更新日期:2015-03-01 00:00:00
abstract::The aim of this study was to evaluate the production performance of Nile tilapia post-larvae and fingerlings fed with increasing levels of alcoholic extract of propolis into diets. In Experiment 1, 1800 post-larvae were distributed in 30 tanks, in a completely randomized design with five treatments composed by the inc...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920180297
更新日期:2019-05-23 00:00:00
abstract::Pollen grain morphology of Bromeliaceae species collected in areas of the Atlantic Rainforest of southeastern Brazil was studied. The following species were analyzed: Aechmea bambusoides L.B.Sm. & Reitz, A. nudicaulis (L.) Griseb., A. ramosa Mart. ex Schult.f., Ananas bracteatus (Lindl.) Schult.f., Billbergia distachi...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201620140715
更新日期:2016-01-01 00:00:00
abstract::The objective was to evaluate the influence of calving and postpartum characteristics, measured in different genetic predominance, on the postpartum rebreeding. Were evaluated 437 partum from cows that received feeding management consisting of a forage base of the native field and the mating season consisted of 90 day...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820171005
更新日期:2018-08-01 00:00:00
abstract::Spatial variation in the environment may be an important source of morphological variation in many organisms. Tadpoles are valuable model organisms for studies of morphological variation, and in particular, the evaluation of the relationship between environmental and morphological variables. In heterogeneous environme...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020181171
更新日期:2020-11-02 00:00:00
abstract::In this work we study the problem of modeling identification of a population employing a discrete dynamic model based on the Richards growth model. The population is subjected to interventions due to consumption, such as hunting or farming animals. The model identification allows us to estimate the probability or the ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652010000400028
更新日期:2010-12-01 00:00:00
abstract::Knowledge about the botanical source of honey is very important for the beekeeper while it indicates adequate and abundant supply sources of nectar and pollen for the bees, thus contributing toward improved yield. The present study means to identify the pollen types occurring in 58 samples of honey produced in two sta...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652007000300003
更新日期:2007-09-01 00:00:00
abstract::Piper aduncum found naturally in the Amazon and southeastern Brazil, is known for its secondary metabolites that have activity on insects. Anticarsia gemmatalis and Spodoptera frugiperda are among the major insect pests associated with agricultural production. This research evaluated the biological activity of hexane,...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720170194
更新日期:2017-07-01 00:00:00
abstract::Rotifers have often been used as indicators of sudden changes in physical and chemical features of the aquatic environment. Such features vary greatly during flood pulse events in small lakes connected to major floodplains. However, few are the studies that investigate the consequences of the flood pulse in rotifer sp...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201420130323
更新日期:2014-09-01 00:00:00
abstract::We investigated the influence of environmental factors in spatial and temporal distribution of the seabob shrimp Xiphopenaeus kroyeri in Santos Bay and São Vicente Estuary, state of São Paulo, Brazil. Monthly samples were obtained, from May 2008 through April 2010, from four locations in the estuary and four in the ba...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201420130230
更新日期:2014-09-01 00:00:00
abstract::The characteristic of immersion, involvement and motivation have made serious games an important tool to be used in the medical field. However, there are people that, having some sort of physical disability, are not able to, or do not feel motivated to play. This work presents a strategy to support human- computer int...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765202020190273
更新日期:2020-02-17 00:00:00
abstract::The interaction of urea with lysozyme was studied in the 192-240 nm spectral region by spectrophotometry. The far and middle ultraviolet protein bands records undergo a non-linear "red shift" and "hypochromic effect" under urea titration. These spectral shifts are interpreted basically in terms of decreasing in molar ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract:OBJECTIVE:the aim of this study is to map thermal stress risks for human health at the São Francisco River Basin (SFRB) in the Semiarid region, for climatic scenarios RCP 4.5 and 8.5. METHODS:The heat stress conditions were defined by the Wet Bulb Globe Temperature (WBGT) indicator and by the average number of annual ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920180748
更新日期:2019-10-07 00:00:00
abstract::A relevant series of symmetric supramolecular porphyrins has been obtained by attaching four [RuII(bipy)2Cl] groups to the pyridyl substituents of meso-tetra(4-pyridyl)porphyrin and its metallated derivatives. These compounds display a rich electrochemistry and versatile catalytic, electrocatalytic and photochemical p...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652000000100003
更新日期:2000-01-01 00:00:00
abstract::The objective of this study was to evaluate the effect of controlled atmosphere (CA) on quality preservation of 'Laetitia' plums, mainly on internal breakdown, in order to determine the best CA storage conditions. Two experiments were carried out one in 2010, and another in 2011. In 2010, besides cold storage (CS; 21....
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201420130237
更新日期:2014-03-01 00:00:00
abstract::Coronary artery calcification is an early marker of subclinical atherosclerosis, but little research has been done in asymptomatic individuals under 45 years. In this cohort study with 17 years of follow-up, 155 participants were assessed in 2016 with a coronary calcium score for the association with cardiovascular ri...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820180052
更新日期:2018-09-01 00:00:00
abstract::In some previous studies, we described the isolation of nine compounds from leaves of Derris urucu, a species found widely in the Amazon rainforest, identified as five stilbenes and four dihydroflavonols. In this work, three of these dihydroflavonols [urucuol A (1), urucuol B (2) and isotirumalin (3)] were evaluated t...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/S0001-37652013000300004
更新日期:2013-09-01 00:00:00
abstract::Miconazole is reduced at mercury electrode above pH 6 involving organometallic compound formation, responsible for an anomalous polarographic behavior. The electrodic process presents a large contribution of the adsorption effects. The drug can be determined by cathodic stripping voltammetry from 8.0 x 10(-8) to 1, 5 ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652002000300005
更新日期:2002-09-01 00:00:00
abstract::Effects of Aspergillus niger was investigated on the strength of cotton textile specimens impregnated with nano-wollastonite, and then compared with normal specimens. Cotton strips were cut and prepared in warp and wept directions according to the standard specifications ASTM D-5035. Results showed that incubation of ...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201820170914
更新日期:2018-09-01 00:00:00
abstract::This study suggests the employment of accumulated sediments in the reservoir of Tucuruí (Pará /Brazil) to remineralize the surrounding degraded soils. The approach was based on the principles of stonemeal technology. It suggests that the soil can be rejuvenated by crushed rocks rich in macro and micronutrients. Remova...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/s0001-37652013000100003
更新日期:2013-03-01 00:00:00
abstract::The present study aimed to assess the accuracy of linear measurements performed with two software packages in multislice (MSCT) and cone beam (CBCT) computed tomography (CT) images. The sample consisted of 10 human mandibles marked standardly 8 times with metallic orthodontic wires. The mandibles underwent both MSCT a...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201720170227
更新日期:2017-10-01 00:00:00
abstract::Hypostomus commersoni Valenciennes 1836, Hypostomus cordovae (Günther 1880) and Hypostomus laplatae (Eigenmann 1907) have been little studied since their original descriptions. This study shows a comprehensive review of these species from the Lower La Plata Basin, including their taxonomic history, distribution, color...
journal_title:Anais da Academia Brasileira de Ciencias
pub_type: 杂志文章
doi:10.1590/0001-3765201920180131
更新日期:2019-01-01 00:00:00