当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE期刊下所有文献 > Orexin A upregulates the protein expression of OX1R and enhances the proliferation of SGC-7901 gastric cancer cells through the ERK signaling pathway.

Orexin A upregulates the protein expression of OX1R and enhances the proliferation of SGC-7901 gastric cancer cells through the ERK signaling pathway.

Abstract：

:Orexins are hypothalamic peptides that regulate food intake, wakefulness, the reward system and energy metabolism. Recent studies have demonstrated the ability of orexins to promote a robust apoptosis and subsequent inhibition of cell growth in various types of cancer cells. The present study was conducted to investigate the effects of orexin A on the survival of human gastric cancer cells, SGC‑7901, and the possible mechanisms. SGC‑7901 cells were exposed to various concentrations of orexin A in vitro in the presence or absence of the orexin receptor 1 (OX1R) antagonist (SB334867), extracellular signal‑regulated kinases 1 and 2 (ERK1/2) antagonist (U0126) or a combination of the two antagonists. The amount of cell proliferation, viability and apoptosis, caspase‑8 and caspases‑9 activities, OX1R protein expression and ERK1/2 protein levels were determined. The expression of OX1R in SGC‑7901 cells was observed. Orexin A (10-10 to 10-6 M) stimulated SGC‑7901 cell proliferation and viability, reduced the pro‑apoptotic activity of caspase‑9 and protected the cells from apoptosis in a dose‑dependent manner. Additionally, ERK1/2 phosphorylation was stimulated by orexin A (10-10 to 10-6 M). However, the OX1R antagonist SB334867 (10-6 M), ERK1/2 antagonist U0126 (30 µM) or the combination of antagonists blocked the effects of orexin A to a certain extent. These results suggest that stimulation of OX1R induces the growth of SGC‑7901 gastric cancer cells through activation of ERK1/2 signaling pathway. These findings add a new dimension to the biological activities of orexin, which may have important implications in health and disease, in particular gastric cancer.

journal_name

Int J Mol Med

journal_title

International journal of molecular medicine

authors

Liu Y,Zhao Y,Ju S,Guo L

doi

10.3892/ijmm.2014.2038

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

539-45

issue

2

eissn

1107-3756

issn

1791-244X

journal_volume

35

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE文献大全
  • miR-214 regulates papillary thyroid carcinoma cell proliferation and metastasis by targeting PSMD10.

    abstract：:MicroRNAs (miRNAs) have important effects on cancer occurrence and development by adjusting gene expression. The aim of the present study was to examine the role of miR‑214 in papillary thyroid carcinoma cell proliferation and metastasis, and its molecular mechanisms. miR‑214 was demonstrated to be markedly downregula...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2018.3902

    authors： Liu F,Lou K,Zhao X,Zhang J,Chen W,Qian Y,Zhao Y,Zhu Y,Zhang Y

    更新日期：2018-12-01 00:00:00

  • MicroRNAs regulate vascular smooth muscle cell functions in atherosclerosis (review).

    abstract：:Vascular smooth muscle cells (VSMCs) are involved in all stages of the progression of human atherosclerosis (AS). MicroRNAs (miRNAs or miRs) are non‑coding, small RNAs that regulate gene expression at the post‑transcriptional level through translational repression or messenger RNA (mRNA) decay. Recently, a variety of ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章,评审

    doi：10.3892/ijmm.2014.1853

    authors： Yu X,Li Z

    更新日期：2014-10-01 00:00:00

  • TNF-α promotes gallbladder cancer cell growth and invasion through autocrine mechanisms.

    abstract：:Tumor necrosis factor-α (TNF-α) has been suggested to be a putative tumor promoter gene, and autocrine of TNF-α expression has been found in colon cancer and ovarian cancer. As the role of autocrine TNF-α in human gallbladder cancer has not yet been elucidated, the present study examined the expression of TNF-α in gal...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2014.1711

    authors： Zhu G,Du Q,Wang X,Tang N,She F,Chen Y

    更新日期：2014-06-01 00:00:00

  • Stanozolol administration combined with exercise leads to decreased telomerase activity possibly associated with liver aging.

    abstract：:Anabolic agents are doping substances which are commonly used in sports. Stanozolol, a 17α‑alkylated derivative of testosterone, has a widespread use among athletes and bodybuilders. Several medical and behavioral adverse effects are associated with anabolic androgenic steroids (AAS) abuse, while the liver remains the...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2018.3644

    authors： Ozcagli E,Kara M,Kotil T,Fragkiadaki P,Tzatzarakis MN,Tsitsimpikou C,Stivaktakis PD,Tsoukalas D,Spandidos DA,Tsatsakis AM,Alpertunga B

    更新日期：2018-07-01 00:00:00

  • Involvement of vascular endothelial growth factor signaling in CLR/RAMP1 and CLR/RAMP2-mediated pro-angiogenic effect of intermedin on human vascular endothelial cells.

    abstract：:Intermedin (IMD) is a recently discovered peptide closely related to adrenomedullin. Its principal physiological activity is its role in the regulation of the cardiovascular system, where it exerts a potent hypotensive effect. In addition, data were recently provided showing that this peptide is able to exert a clearc...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm_00000464

    authors： Albertin G,Sorato E,Oselladore B,Mascarin A,Tortorella C,Guidolin D

    更新日期：2010-08-01 00:00:00

  • Effects of hexose pentaacetates on electrical activity and cytosolic Ca2+ in mouse pancreatic islets.

    abstract：:Electrical activity of beta-cells and cytosolic Ca2+ concentration ([Ca2+]i) were monitored in mouse pancreatic islets exposed to the pentaacetate esters of alpha-D-glucose, beta-D-galactose and beta-L-glucose, all tested at 1.7 mM concentration. In the presence of 5 mM D-glucose, alpha-D-glucose pentaacetate induced ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.3.1.15

    authors： Pomares R,Ropero AB,Sánchez-Andrés JV,Nadal A,Soria B,Malaisse WJ

    更新日期：1999-01-01 00:00:00

  • IL‑27 suppresses airway inflammation, hyperresponsiveness and remodeling via the STAT1 and STAT3 pathways in mice with allergic asthma.

    abstract：:Type 2 cytokine‑associated immunity may be involved in the pathogenesis of allergic asthma. Although interleukin 27 (IL‑27) has been reported as an initiator and suppressor of T‑helper 1 (Th1) and T‑helper 2 (Th2) responses, respectively, its effects on the development of asthma remain unclear. In the present study, m...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2020.4622

    authors： Lu D,Lu J,Ji X,Ji Y,Zhang Z,Peng H,Sun F,Zhang C

    更新日期：2020-08-01 00:00:00

  • Insulinoma-associated protein 2-deficient mice develop severe forms of diabetes induced by multiple low doses of streptozotocin.

    abstract：:Insulinoma-associated protein 2 (IA-2) is the major autoantigen that contributes to the pathogenesis of type 1 diabetes (T1D). IA-2-deficient (IA-2-/-) mice showed impaired insulin secretion after intraperitoneal injection of glucose as well as elevated glucose level in a glucose tolerance test. Despite the fact that ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm_00000201

    authors： Nakajima K,Wu G,Takeyama N,Sakudo A,Sugiura K,Yukawa M,Onodera T

    更新日期：2009-07-01 00:00:00

  • Low vitamin D-modulated calcium-regulating proteins in psoriasis vulgaris plaques: S100A7 overexpression depends on joint involvement.

    abstract：:Psoriasis is an inflammatory skin disease with or without joint involvement. In this disease, the thickened epidermis and impaired barrier are associated with altered calcium gradients. Calcium and vitamin D are known to play important roles in keratinocyte differentiation and bone metabolism. Intracellular calcium is...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2016.2718

    authors： Cubillos S,Norgauer J

    更新日期：2016-10-01 00:00:00

  • A novel NOTCH3 mutation identified in patients with oral cancer by whole exome sequencing.

    abstract：:Oral cancer is a serious disease caused by environmental factors and/or susceptible genes. In the present study, in order to identify useful genetic biomarkers for cancer prediction and prevention, and for personalized treatment, we detected somatic mutations in 5 pairs of oral cancer tissues and blood samples using w...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2017.2965

    authors： Yi Y,Tian Z,Ju H,Ren G,Hu J

    更新日期：2017-06-01 00:00:00

  • Identification of sex hormone binding globulin-interacting proteins in the brain using phage display screening.

    abstract：:The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat prot...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm_00000248

    authors： Gnanasekar M,Suleman FG,Ramaswamy K,Caldwell JD

    更新日期：2009-10-01 00:00:00

  • Regulation of vascular smooth muscle proliferation and migration by beta2-chimaerin, a non-protein kinase C phorbol ester receptor.

    abstract：:The proliferation and migration of vascular smooth muscle cells (SMC) are important aspects of atherogenesis. Activated growth factor signaling in injured vessels subsequently promotes a number of intracellular events resulting in the phenotypic modulation of SMC. Here, we investigated the role of beta2-chimaerin, a n...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：

    authors： Maeda M,Kato S,Fukushima S,Kaneyuki U,Fujii T,Kazanietz MG,Oshima K,Shigemori M

    更新日期：2006-04-01 00:00:00

  • CDC25A inhibition suppresses the growth and invasion of human hepatocellular carcinoma cells.

    abstract：:CDC25A is a cell cycle-activating phosphatase that promotes transition from the G1 to S phase. We previously reported that overexpression of CDC25A in human hepatocellular carcinoma (HCC) tissue samples was associated with poor prognosis. In this study, we attempted suppression of CDC25A in HCC cells to elucidate the ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：

    authors： Xu X,Yamamoto H,Liu G,Ito Y,Ngan CY,Kondo M,Nagano H,Dono K,Sekimoto M,Monden M

    更新日期：2008-02-01 00:00:00

  • SIRT1 mediates the role of RNA-binding protein QKI 5 in the synthesis of triglycerides in non-alcoholic fatty liver disease mice via the PPARα/FoxO1 signaling pathway.

    abstract：:Non‑alcoholic fatty liver disease (NAFLD) is the consequence of insulin resistance, fatty acid accumulation, oxidative stress and lipotoxicity. The present study aimed to elucidate the effect of Quaking 5 (QKI 5) as mediated by Sirtuin 1 (SIRT1) on triglyceride (TG) synthesis in the liver of an NAFLD mouse model. A hi...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2019.4059

    authors： Zhang W,Sun Y,Liu W,Dong J,Chen J

    更新日期：2019-03-01 00:00:00

  • Cannabinoid receptor type 1 mediates high-fat diet-induced insulin resistance by increasing forkhead box O1 activity in a mouse model of obesity.

    abstract：:Hepatic glucose production is promoted by forkhead box O1 (FoxO1) under conditions of insulin resistance. The overactivity of cannabinoid receptor type 1 (CB1R) partly causes increased liver fat deposits and metabolic dysfunction in obese rodents by decreasing mitochondrial function. The aim of the present study was t...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2016.2475

    authors： Chen CC,Lee TY,Kwok CF,Hsu YP,Shih KC,Lin YJ,Ho LT

    更新日期：2016-03-01 00:00:00

  • MicroRNA‑16 inhibits endometrial stromal cell migration and invasion through suppression of the inhibitor of nuclear factor‑κB kinase subunit β/nuclear factor‑κB pathway.

    abstract：:Accumulating evidence has demonstrated that endometrial stromal cells (ESCs) are responsible for the pathogenesis of endometriosis (Ems), which is characterized by the presence of functional endometrial‑like tissues outside the uterine cavity. Abnormal expression of microRNAs (miRNAs) in ESCs may be implicated in the ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2020.4620

    authors： Wang X,Ren R,Shao M,Lan J

    更新日期：2020-08-01 00:00:00

  • Vaccination with embryonic stem cells generates effective antitumor immunity against ovarian cancer.

    abstract：:To date, only a few studies have suggested that human embryonic stem cells (hESCs) might effectively immunize against colon and lung cancer. The purpose of this study was to investigate the therapeutic potential of hESCs as a vaccine to induce widespread antitumor effects in differe...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2012.1195

    authors： Zhang Z,Chen X,Chang X,Ye X,Li Y,Cui H

    更新日期：2013-01-01 00:00:00

  • Transcriptome and methylome profiling in a rat model of irritable bowel syndrome induced by stress.

    abstract：:Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is associated with psychological stress. However, the full landscape of IBS‑related epigenetic factors remains unveiled and needs to be elucidated. The water‑avoidance stress (WAS) method was used to induce a rat IBS model. Each rat was monitore...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2018.3823

    authors： Zhu S,Min L,Guo Q,Li H,Yu Y,Zong Y,Wang L,Li P,Gu J,Zhang S

    更新日期：2018-11-01 00:00:00

  • Metformin exerts anticancer effects through the inhibition of the Sonic hedgehog signaling pathway in breast cancer.

    abstract：:Metformin, a widely prescribed antidiabetic drug, has previously been shown to lower the risk of certain types of cancer, including that of breast cancer, and to improve prognosis. Its anticancer effects, which are mediated by the activation of AMP-activated protein kinase (AMPK), have become notable. The Sonic hedgeh...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2015.2217

    authors： Fan C,Wang Y,Liu Z,Sun Y,Wang X,Wei G,Wei J

    更新日期：2015-07-01 00:00:00

  • Characterization of the nuclear localization signal in the DNA helicase responsible for Bloom syndrome.

    abstract：:Bloom syndrome (BS) is a rare genetic disorder characterized by small body size, photosensitivity, immunodeficiency and a high predisposition to various types of cancer. BLM was identified as the causative gene for BS. The BLM protein is homologous to DNA helicase and has two basic amino acid clusters in its C-termina...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.5.5.477

    authors： Hayakawa S,Kaneko H,Fukao T,Kasahara K,Matsumoto T,Furuichi Y,Kondo N

    更新日期：2000-05-01 00:00:00

  • Effects of preproglucagon-derived peptides and exendins on steroid-hormone secretion from dispersed adrenocortical cells of normal and streptozotocin-induced diabetic rats.

    abstract：:Many lines of evidence have shown that preproglucagon-derived peptides affect steroid secretion from dispersed adrenocortical cells, and that streptozotocin (STZ)-induced experimental diabetes alters adrenocortical-cell function. Hence, we compared the effects of glucagon, glucagon-like peptide (GLP)-1 and GLP-2 on ba...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：

    authors： Malendowicz LK,Spinazzi R,Nussdorfer GG,Trejter M

    更新日期：2003-07-01 00:00:00

  • Liver X receptor and retinoic X receptor agonists modulate the expression of genes involved in lipid metabolism in human endothelial cells.

    abstract：:The cooperation of liver X receptors (LXRs) alpha and beta, and retinoic X receptor (RXR) modulate the expression of several genes involved in lipid metabolism in hepatocyte and macrophages. Using cDNA microarray technology, we have shown previously that several of these genes are also expressed in endothelial cells. ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：

    authors： Norata GD,Ongari M,Uboldi P,Pellegatta F,Catapano AL

    更新日期：2005-10-01 00:00:00

  • Calcium signalling and control of cell proliferation by tyrosine kinase receptors (review).

    abstract：:Most peptidic growth factors (GFs) that act as mitogens for different cell types bind to tyrosine kinase receptors (RTKs) and trigger complex intracellular signal transduction pathways finally leading to cell proliferation. Among the early events induced by GFs, cytosolic calcium increase plays a key role, and in part...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章,评审

    doi：

    authors： Munaron L

    更新日期：2002-12-01 00:00:00

  • Role of Rho kinase in lysophosphatidic acid-induced altering of blood-brain barrier permeability.

    abstract：:Lysophosphatidic acid (LPA) the simplest of the water-soluble phospholipids, is produced by activated platelets, macrophage and endothelial cells. It also evokes various biological responses. When LPA concentrations reach high levels, brain injury, including stroke and intracerebral hemorrhage (ICH), occurs. Previous ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.2014.1618

    authors： Yu Y,Qin J,Liu M,Ruan Q,Li Y,Zhang Z

    更新日期：2014-03-01 00:00:00

  • Focal cortical dysplasia: Molecular disturbances and clinicopathological classification (Review).

    abstract：:Focal cortical dysplasia (FCD) is one of the most important causes of drug-resistant epilepsy in paediatric patients, particularly in those below the age of 3. Even though over 40 years have passed since the first description of the entity by Taylor, the exact mechanisms causing these cortical abnormalities remain une...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章,评审

    doi：10.3892/ijmm.2016.2760

    authors： Siedlecka M,Grajkowska W,Galus R,Dembowska-Bagińska B,Jóźwiak J

    更新日期：2016-11-01 00:00:00

  • Decreased expression of claudin-1 correlates with recurrence status in breast cancer.

    abstract：:Claudins (CLDNs) constitute the major transmembrane proteins of tight junctions. It may be hypothesized that changes in or loss of expression of tight junctional proteins such as CLDNs can lead to cellular disorientation and detachment, which is commonly seen in neoplasia. Recent studies have suggested that claudin-1 ...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：

    authors： Morohashi S,Kusumi T,Sato F,Odagiri H,Chiba H,Yoshihara S,Hakamada K,Sasaki M,Kijima H

    更新日期：2007-08-01 00:00:00

  • Experimental postoperative adjuvant chemotherapy by UFT using primary tumor amputation model.

    abstract：:We evaluated the postoperative adjuvant chemotherapy by UFT using the primary tumor amputation-pulmonary metastasis model. When Lewis lung carcinoma (LLC) primary tumors on the hind foot pad grew palpable, they were amputated on two different days. In experiment (A) (earlier amputation model), micrometastases were det...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.5.4.357

    authors： Uchida J,Sato K,Okabe H,Saito H,Nakano K,Fujioka A,Takechi T,Takeda S,Unemi N,Fukushima M

    更新日期：2000-04-01 00:00:00

  • Genetic variations of the midkine (MK) gene in human sporadic colorectal and gastric cancers.

    abstract：:Midkine (MK), a retinoic acid responsible protein, is regulated during development and may play an important role in tumorigenesis. A search for genetic variations of the MK gene, located on chromosome 11q11.2 in humans, has not yet been conducted in cancers. To examine the entire coding region, as well as 4 regions o...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.6.3.281

    authors： Ahmed KM,Shitara Y,Kuwano H,Takenoshita S,Uchimuro K,Shinozawa T

    更新日期：2000-09-01 00:00:00

  • Association of genetic variants with myocardial infarction in Japanese individuals with different lipid profiles.

    abstract：:Dyslipidemia is an important risk factor for myocardial infarction (MI). We previously showed that gene polymorphisms associated with MI differed among individuals with different lipid profiles. We further examined whether genetic variants that confer susceptibility to MI might differ among individuals with low or hig...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm_00000383

    authors： Yoshida T,Kato K,Yokoi K,Oguri M,Watanabe S,Metoki N,Yoshida H,Satoh K,Aoyagi Y,Nozawa Y,Yamada Y

    更新日期：2010-04-01 00:00:00

  • BCL-2 expression and inhibin-A in adrenal neoplasms: a comparative study.

    abstract：:Differential diagnosis between adrenal cortical and adrenal medullary lesions may be difficult in many cases. Different immunohistochemical, histochemical tools as well as ultrastructural diagnostic techniques have been employed to aid in differentiating between these lesions. Recently, both inhibin-A and BCL-2 have b...

    journal_title：International journal of molecular medicine

    pub_type： 杂志文章

    doi：10.3892/ijmm.3.3.271

    authors： Fogt F,Zimmerman RL,Mulligan N,Vortmeyer AO,Poremba C,Harris CA,Alsaigh N,Tomaszewski JE,Nikulasson ST

    更新日期：1999-03-01 00:00:00