Abstract:
:Electrical activity of beta-cells and cytosolic Ca2+ concentration ([Ca2+]i) were monitored in mouse pancreatic islets exposed to the pentaacetate esters of alpha-D-glucose, beta-D-galactose and beta-L-glucose, all tested at 1.7 mM concentration. In the presence of 5 mM D-glucose, alpha-D-glucose pentaacetate induced electrical activity and increased [Ca2+]i, whilst beta-D-galactose pentaacetate failed to do so. The electrical and cationic response to the D-glucose ester occurred with a delay of between 5 and 10 min, the ester-induced increase in [Ca2+]i being suppressed in the absence of extracellular Ca2+. As a rule, beta-L-glucose pentaacetate also failed to evoke biophysical responses in the islets exposed to 5 mM D-glucose. However, in the presence of 10 mM L-leucine the L-glucose ester induced electrical activity. These findings, which parallel the insulinotropic action of the same esters in rat pancreatic islets reinforce the view that the positive insulinotropic action of selected hexose pentaacetates cannot be attributed to the catabolism of their acetate moiety but, instead, involves a dual mode of action linked to both the metabolism of their carbohydrate moiety and a direct effect of the ester itself upon a yet unidentified receptor system. Furthermore, this study provides the first evidence that the latter direct effect results in the induction of both electrical activity and [Ca2+]i oscillations.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Pomares R,Ropero AB,Sánchez-Andrés JV,Nadal A,Soria B,Malaisse WJdoi
10.3892/ijmm.3.1.15subject
Has Abstractpub_date
1999-01-01 00:00:00pages
15-20issue
1eissn
1107-3756issn
1791-244Xjournal_volume
3pub_type
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