Abstract:
:Abnormal gap junction communication (GJC) has been associated with carcinogenesis. We investigated the role of endothelial cell GJC and connexin 43 (Cx43), the main gap junction protein in these cells, during tumour cell extravasation. GJC was determined by the ability of cells to transfer Lucifer yellow, to neighbouring cells. Tumour-endothelial interaction was assessed by DiI assay. Connexin 43 expression and tyrosine phosphorylation were measured by immunoprecipitation and Western blotting. Co-culturing of ECV304 endothelial cells with human breast cancer cells resulted in a rapid and transient loss of communication competence of ECV304. This inhibition was maximal within 5 min. GJC was almost fully restored in 2 h. The co-culturing also resulted in an increase in the tyrosine phosphorylation of CX43. The pattern of phosphorylation was similar to the loss and the recovery of GJC in ECV304. We conclude that interaction of tumour cells with endothelium effectively inhibits GJC of endothelial cells, which is attributed to the increased tyrosine phosphorylation of connexin 43. This may contribute to the extravasation of tumour cells from the circulation, an essential step in the establishment of metastasis.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Cai J,Jiang WG,Mansel REsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
273-8issue
1eissn
1107-3756issn
1791-244Xjournal_volume
1pub_type
杂志文章abstract::Selenium deficiency is a causative factor in heart failure and microRNAs (known as miRNAs or miRs) play an important role in numerous cardiovascular diseases. However, the changes of miRNA expression during selenium deficiency and whether selenium deficiency is involved in cardiac dysfunction remain unclear. In the pr...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2014.1976
更新日期:2015-01-01 00:00:00
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
pub_type: 杂志文章,已发布勘误
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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更新日期:2005-10-01 00:00:00
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journal_title:International journal of molecular medicine
pub_type: 杂志文章,评审
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journal_title:International journal of molecular medicine
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