Abstract:
:The present study reports the identification of human sex hormone binding globulin (SHBG)-interacting proteins in the brain using a phage display-based screening technology. Phage display is a system in which a foreign protein is displayed on the surface of a bacteriophage as a fusion protein with one of the coat proteins of the bacteriophage. T7 phage clones expressing normal human brain proteins (human normal brain phage-display cDNA expression library) were screened using SHBG as bait. The bound phage clones were then identified by DNA sequencing and by BLAST search analysis. Of the twenty binding proteins analyzed, three were found to be membrane-associated proteins: synaptosomal associated protein 25 (SNAP25), Thy-1 cell surface antigen and zonadhesin. Further studies will determine if the interactions of SHBG with these proteins have any role in the internalization and cell signaling events or whether they contribute to steroid delivery to specific cells.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Gnanasekar M,Suleman FG,Ramaswamy K,Caldwell JDdoi
10.3892/ijmm_00000248subject
Has Abstractpub_date
2009-10-01 00:00:00pages
421-6issue
4eissn
1107-3756issn
1791-244Xjournal_volume
24pub_type
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