Abstract：
:Although recent reports indicate an increasing incidence of patients with reflux esophagitis, its pathomechanism remains unclear. Cytokines and neutrophils, the latter of which produce reactive oxygen species (ROS), have been implicated in the formation of gastrointestinal diseases. This study investigated the roles of neutrophils, ROS, and cytokines in the pathogenesis of experimental reflux esophagitis. Esophagitis was induced in male Wistar rats by ligation at both the limiting ridge of the stomach and lower portion of the duodenum. The esophagus was then removed, and the lesion index, wet weight, thiobarbituric acid-reactive substances (an index of lipid peroxidation), myeloperoxidase activity (an index of neutrophil accumulation), tumor necrosis factor-alpha (TNF-alpha), and cytokine-induced neutrophil chemoattractant (CINC)-1 in the esophageal mucosa were estimated, and a histological study (hematoxylin-and-eosin staining) was performed. The mRNA expression of TNF-alpha and CINC-1 was analyzed. Anti-neutrophil serum (ANS) was injected intraperitoneally prior to the induction of esophagitis, and inflammatory markers were estimated as described above. The values of all markers increased, and the histological study revealed neutrophil infiltration and edema in mucosa and submucosa at both 12 and 18 h after induction. However, the mRNA expression of both cytokines was observed earlier at 3 and 6 h after induction. ANS inhibited the increases in all inflammatory markers. These results indicate that ROS and lipid peroxidation mainly derived from neutrophils, which are stimulated and mobilized by TNF-alpha and CINC-1, are implicated in the pathogenesis of esophageal inflammation induced by the reflux of gastroduodenal contents.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Yamaguchi T,Yoshida N,Tomatsuri N,Takayama R,Katada K,Takagi T,Ichikawa H,Naito Y,Okanoue T,Yoshikawa Tsubject
Has Abstractpub_date
2005-07-01 00:00:00pages
71-7issue
1eissn
1107-3756issn
1791-244Xjournal_volume
16pub_type
杂志文章abstract：:Colon cancer stem cells (CCSCs) are involved in colon cancer and promote tumor progression and recurrence. LGR5, a marker for intestinal stem cells (ISCs), is also considered to serve as a marker for CCSCs. However, the precise function of LGR5 in CCSCs is unclear. In this study, we demonstrated that LGR5 was highly e...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1752
更新日期：2014-07-01 00:00:00
abstract：:Antigen presenting cells, especially the antigen presenting dendritic cells (DC) in the tissue, regulate the magnitude of antigen-specific immune response. A role of impaired and narrowly focused specific immune response has been implicated in the pathogenesis of chronic hepatitis due to hepatitis B virus and hepatiti...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) is a key coordinator of gene programs in metabolism and energy homeostasis in mammals. The aim of this study was to determine whether PGC-1α is involved in the transcriptional regulation of retinal neovascularization in oxygen-induced retinopathy (OI...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1717
更新日期：2014-06-01 00:00:00
abstract：:The purpose of this study is to extract common features of an environmental hormone-oriented neoplasia by comparatively investigating the epidemiological characteristics of testicular cancer in Denmark with those of other cancers, of which the age-adjusted incidence rates (AAIRs) underwent remarkable increase or decre...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2.6.705
更新日期：1998-12-01 00:00:00
abstract：:Noise-induced hearing loss (NIHL) is one of the most frequent disabilities in industrialized countries. It has been demonstrated that hair cell loss in the auditory end organ may account for the majority of ear pathological conditions. Previous studies have indicated that histone deacetylases (HDACs) play an important...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2015.2236
更新日期：2015-08-01 00:00:00
abstract：:Adult mesenchymal progenitor cells (MPCs) are adherent stromal cells of non-haematopoietic origin derived from bone marrow and other tissues. Upon limited in vitro expansion, they retain their self-renewal capacity as well as their potential to differentiate into tissues of mesenchymal lineage, such as bone, cartilage...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm_00000188
更新日期：2009-06-01 00:00:00
abstract：:Xuebijing injection (XBJ) has long been used to treat infectious diseases in China. The therapeutic effect of XBJ is probably associated with anti-inflammatory effects. However, the precise mechanisms responsible for the effects of XBJ remain unknown. The present study was conducted in order to evaluate the protective...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2016.2749
更新日期：2016-11-01 00:00:00
abstract：:Induction of apoptosis is one of the mechanisms of chemotherapeutic agents against breast cancer. In addition, recent studies have shown that diets containing polyphenolic components possess anticancer activities either in vitro or in vivo by inhibiting cell proliferation and inducing apoptosis. The aim of our study w...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：:Cell replacement and gene therapy using neural stem cells (NSCs) have been widely touted as a promising treatment for CNS diseases including brain tumors. Histone deacetylase (HDAC) inhibitors have been used to explore mechanisms behind the lineage-specific differentiation of NSCs a...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2011.791
更新日期：2011-12-01 00:00:00
abstract：:In the present study, high-resolution magic-angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was applied to live Pseudomonas aeruginosa (PA) bacterial cells to determine the metabolome of this opportunistic Gram-negative human pathogen, and in particular, its response to the volatile aromatic low m...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3760
更新日期：2018-10-01 00:00:00
abstract：:A preceding hypoxic insult can sensitize the cells or the organism to a subsequent, second insult. The aim of the present study was to investigate the molecular mechanism of this phenomenon (often termed 'two-hit' injury paradigm), in an in vitro model of hypoxia/oxidative stress injury in A549 epithelial cells, with ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1397
更新日期：2013-08-01 00:00:00
abstract：:Aberrant DNA methylation on CpG islands is one of the most consistent epigenetic changes in human cancers, and the methylation process is catalyzed by DNA methyltransferase (DNMT). We evaluated i) the mRNA levels of three DNMTs; DNMT1, DNMT3a and DNMT3b, in 25 hepatocellular carcinomas (HCCs), in their corresponding n...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：:Chronic hepatitis B (CHB) virus infection is caused by compromised host immunity, but the precise underlying mechanism remains unclear. Retinoic acid-inducible gene I (RIG-I) triggers antiviral immunity by inducing interferon-β (IFN-β) production following viral infection. To investigate the role of the RIG-I-IFN-β si...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2012.1131
更新日期：2012-12-01 00:00:00
abstract：:Sindbis vectors have shown remarkable antitumor efficacy and tumor-targeting capacity in animal models and hold promise for cancer therapy. Different packaging systems are used to produce propagation-incompetent Sindbis vectors. However, the vectors produced using either DH-BB single helper RNA or split helper RNA can...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1395
更新日期：2013-08-01 00:00:00
abstract：:Cardiac fibroblast (CF) proliferation and transformation into myofibroblasts play important roles in cardiac fibrosis during pathological myocardial remodeling. In this study, we demonstrate that hepatocyte growth factor (HGF), an antifibrotic factor in the process of pulmonary, renal and liver fibrosis, is a negative...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1782
更新日期：2014-08-01 00:00:00
abstract：:The ZNF268 gene was originally isolated from an early human embryo cDNA library. Several different transcripts have been isolated for the ZNF268 gene and developmental expression studies suggest that ZNF268 plays a role in the development of human fetal liver and the differentiation of blood cells. In our effort to st...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:Hyperosmotic stress may be initiated during a diverse range pathological circumstances, which in turn results in tissue damage. In this process, the activation of survival signaling, which has the capacity to restore cell homeostasis, determines cell fate. Autophagy is responsible for cell survival and is activated by...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3873
更新日期：2018-12-01 00:00:00
abstract：:Connective tissue growth factor (CTGF) plays an important role in the pathogenesis of atherosclerosis by promoting vascular smooth muscle cell (VSMC) growth, migration, apoptosis, adhesion and the secretion of matrix components. The osteogenic differentiation of VSMCs is essential in the development of vascular calcif...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1398
更新日期：2013-08-01 00:00:00
abstract：:The immunosuppressive effect of triptolide has been associated with suppression of T-cell activation. However, the immunosuppressive effects of triptolide on innate immunity in the epithelial barrier remain to be elucidated. Human beta-defensin (HBD)-2 is an inducible antimicrobial peptide and plays an important role ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：:Selenium is an essential trace element and has shown chemopreventive or therapeutic activities on human solid cancers; however, whether it has anticancer effects on leukemia has not yet been elucidated. The present study was designed to determine the role of selenium on HL-60 human promyelocytic leukemia cells. We fou...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1854
更新日期：2014-10-01 00:00:00
abstract：:Neural stem cell transplantation is a promising new treatment of ischemic or traumatic brain injury. We have now investigated the involvement of the peroxynitrite - poly(ADP-ribose) polymerase (ONOO- - PARP) activation cascade in brain trauma and neural stem cell transplantation. The forelimb motor cortex of adult mal...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：:When treating glaucoma, excessive scar tissue reactions reduce the postoperative survival rate of the filtering bleb. Accumulating evidence has demonstrated that the proliferation, migration and extracellular matrix (ECM) synthesis of fibroblasts are important molecular mechanisms underlying scar formation. Recent evi...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3323
更新日期：2018-03-01 00:00:00
abstract：:CDC25A is a cell cycle-activating phosphatase that promotes transition from the G1 to S phase. We previously reported that overexpression of CDC25A in human hepatocellular carcinoma (HCC) tissue samples was associated with poor prognosis. In this study, we attempted suppression of CDC25A in HCC cells to elucidate the ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2008-02-01 00:00:00
abstract：:Regucalcin is a Ca2+-binding protein which plays a regulatory role in liver cell functions related to Ca2+. In this study we have cloned and characterized cDNA for regucalcin from human liver and human hepatoma cell line Hep G2 by screening and rapid amplification of cDNA ends (RACE). The nucleotide sequences of the c...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:For tumor progression, a cascade of linked sequential biological events is essential. We tried to test whether biological therapy can modulate specific biological phenotypes and increase the anti-tumor effect when combined with chemotherapy. Five human gastric cancer cell lines (YCC-1, YCC-2, YCC-3, YCC-7, AGS) were u...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.4.2.203
更新日期：1999-08-01 00:00:00
abstract：:Choroidal neovascularization (CNV) can lead to progressive and severe visual loss. Vascular endothelial growth factor (VEGF) promotes the development of CNV. Caffeic acid phenethyl ester (CAPE), a biologically active component of the honeybee (Apis mellifera) propolis, has been demonstrated to have several interesting...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2015.2116
更新日期：2015-05-01 00:00:00
abstract：:The aim of the present study was to determine whether osteoprotegerin (OPG) influences the bone resorption activity of osteoclasts. RAW264.7 cells were induced by macrophage colony-stimulating factor (M-CSF) + receptor activator of nuclear factor-κB ligand (RANKL) and 0, 10, 20, 50 and 100 ng/ml OPG were added into va...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1329
更新日期：2013-06-01 00:00:00
abstract：:Microglia of the central nervous system serve a variety of functions that may ultimately lead to the development or detriment of neighboring neuronal and vascular cells. These scavengers of the nervous system have been associated with a variety of neurodegenerative disorders, but the toxic potential of microglia is eq...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2007-02-01 00:00:00
abstract：:As a DNA repair protein, flap endonuclease 1 (FEN1), a structure-specific 5' nuclease, plays pivotal roles in the maturation of Okazaki fragments, long-patch base excision repair, restarting of stalled replication forks and telomere maintenance. FEN1 possesses 5' endonuclease, 5' exonuclease and gap-endonuclease activ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1682
更新日期：2014-05-01 00:00:00
abstract：:We have recently demonstrated the antiproliferative and apoptotic activities of herbal traditional Chinese medicines, including the analomous fruit extract of Gleditsia sinensis, the fresh juice of Scutellaria barbata and the warmed water extract of Radix Sophorae Tonkinensis on a series of human carcinoma cells. Here...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
