Abstract:
:CDC25A is a cell cycle-activating phosphatase that promotes transition from the G1 to S phase. We previously reported that overexpression of CDC25A in human hepatocellular carcinoma (HCC) tissue samples was associated with poor prognosis. In this study, we attempted suppression of CDC25A in HCC cells to elucidate the therapeutic potential of this approach. Administration of CDC25A antisense (AS) oligonucleotide resulted in 25-50% inhibition of cell growth at 48 h, G0-G1 arrest, and significant inhibition of cancer cell invasion. To elucidate the underlying mechanism of the inhibitory effects of HCC cell invasion, we examined several invasion-associated molecules, and we found that membrane-type 3 (MT3)-matrix metalloproteinase (MMP) mRNA was greatly reduced following treatment with AS oligonucleotide to CDC25A or siRNA treatment. Notably, screening of a panel of gastrointestinal cancer cells indicated that MT3-MMP was generally expressed by HCC cells, whereas other cell types did not express this type of matrix metalloproteinase so frequently. We also found that CDC25A facilitated cellular differentiation by increasing albumin expression in the PLC cell line. These results suggest that CDC25A, by inhibiting HCC growth and invasion, may be a feasible therapeutic target for human HCC.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Xu X,Yamamoto H,Liu G,Ito Y,Ngan CY,Kondo M,Nagano H,Dono K,Sekimoto M,Monden Msubject
Has Abstractpub_date
2008-02-01 00:00:00pages
145-52issue
2eissn
1107-3756issn
1791-244Xjournal_volume
21pub_type
杂志文章abstract::White blood cell (WBC) count is considered a prognostic risk factor in acute myeloid leukemia. As density of leukemic cells increases, the cytotoxic activity of certain anticancer drugs, such as vincristine and doxorubicin, progressively decreases. In this study, we investigated the cell density-dependent induction of...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm_00000277
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2011.751
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2015.2160
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journal_title:International journal of molecular medicine
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更新日期:2002-11-01 00:00:00
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2016.2475
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doi:10.3892/ijmm.2018.3909
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2018.3972
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2018.3515
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journal_title:International journal of molecular medicine
pub_type: 杂志文章,评审
doi:
更新日期:2007-09-01 00:00:00
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2020.4836
更新日期:2021-03-01 00:00:00
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2018.3939
更新日期:2019-01-01 00:00:00
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2012.1037
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Atopic dermatitis (AD) shows an increased susceptibility to Staphylococcus aureus infection partly due to decreased expression of human beta-defensin-2 (HBD-2). Interestingly, it was reported that the nasal carrier S. aureus down-regulates the expression of HBD-2 and -3, thereby the carrier strains of S. aureus retain...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm_00000135
更新日期:2009-03-01 00:00:00
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2012.943
更新日期:2012-06-01 00:00:00
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2014.2004
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journal_title:International journal of molecular medicine
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更新日期:2011-11-01 00:00:00
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journal_title:International journal of molecular medicine
pub_type: 杂志文章,评审
doi:10.3892/ijmm.4.5.549
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