Flap endonuclease 1 is a promising candidate biomarker in gastric cancer and is involved in cell proliferation and apoptosis.


:As a DNA repair protein, flap endonuclease 1 (FEN1), a structure-specific 5' nuclease, plays pivotal roles in the maturation of Okazaki fragments, long-patch base excision repair, restarting of stalled replication forks and telomere maintenance. FEN1 possesses 5' endonuclease, 5' exonuclease and gap-endonuclease activities, which render it an essential node in maintaining genome fidelity. The aim of this study was to investigate the association between the expression level of FEN1 and gastric cancer and to explore the role of FEN1 in carcinogenesis and the progression of gastric cancer. The mRNA and protein expression of FEN1 in 42 matched pairs of human gastric tumor tissues and corresponding normal tissues were measured by semiquantitative reverse transcription-PCR and immunohistochemical staining. FEN1 expression was downregulated in the SGC-7901 gastric cancer cells following transfection with siRNA targeting the FEN1 gene. Western blot analysis was used to evaluate the protein expression of FEN1 in SGC-7901 human gastric cancer cells in order to verify the transfection efficiency of FEN1 siRNA. Moreover, cell proliferation was analyzed by MTS assay. The apoptosis of the cells was determined by flow cytometry. Our results revealed that FEN1 was overexpressed in gastric cancer in comparison to the corresponding normal gastric tissues (P<0.01). We further confirmed that FEN1 expression has a positive correlation with the degree of differentiation (P=0.027), lymphatic metastasis (P=0.001), tumor size (P=0.026) and TNM stage (P=0.020) of gastric cancer. A high FEN1 expression in SGC-7901 cells can be effectively downregulated by siRNA constructed to target the FEN1 gene. Moreover, the inhibition of FEN1 expression suppressed the proliferation and induced the apoptosis of SGC-7901 cells. Taken together, our results indicate that FEN1 may be a promising biomarker for the diagnosis of gastric cancer and individual therapy.


Int J Mol Med


Wang K,Xie C,Chen D




Has Abstract


2014-05-01 00:00:00












  • Melatonin improves experimental colitis with sleep deprivation.

    abstract::Sleep deprivation (SD) is an epidemic phenomenon in modern countries, and its harmful effects are well known. SD acts as an aggravating factor in inflammatory bowel disease. Melatonin is a sleep-related neurohormone, also known to have antioxidant and anti-inflammatory effects in the gastrointestinal tract; however, t...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Park YS,Chung SH,Lee SK,Kim JH,Kim JB,Kim TK,Kim DS,Baik HW

    更新日期:2015-04-01 00:00:00

  • TNF-α promotes gallbladder cancer cell growth and invasion through autocrine mechanisms.

    abstract::Tumor necrosis factor-α (TNF-α) has been suggested to be a putative tumor promoter gene, and autocrine of TNF-α expression has been found in colon cancer and ovarian cancer. As the role of autocrine TNF-α in human gallbladder cancer has not yet been elucidated, the present study examined the expression of TNF-α in gal...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Zhu G,Du Q,Wang X,Tang N,She F,Chen Y

    更新日期:2014-06-01 00:00:00

  • Long non‑coding RNA urothelial cancer associated 1 regulates radioresistance via the hexokinase 2/glycolytic pathway in cervical cancer.

    abstract::Cervical cancer is one of the most common types of female malignant tumor. It is well established that radiotherapy (RT) is the first‑line treatment of cervical cancer; however, radioresistance is a substantial obstacle to cervical cancer RT. At present, the mechanism underlying radioresistance remains unclear. Emergi...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Fan L,Huang C,Li J,Gao T,Lin Z,Yao T

    更新日期:2018-10-01 00:00:00

  • A transgenic strategy for analyzing the regulatory regions of the human prostate-specific antigen gene: potential applications for the treatment of prostate cancer (Review).

    abstract::Prostate-specific antigen (PSA) has been used clinically as a marker for the diagnosis and staging of prostate cancer due to its specific expression in prostate epithelial cells. In addition to its medical importance, its complex hormonal and tissue-specific regulation makes it an attractive model to study gene regula...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章,评审


    authors: Willis RA,Wei C,Turner MJ,Callahan BP,Pugh AE,Barth RK,Lord EM,Frelinger JG

    更新日期:1998-02-01 00:00:00

  • The mechanisms of antitumor effects of luteinizing hormone-releasing hormone agonist (buserelin) in 7, 12-dimethylbenz(a)anthracene-induced rat mammary cancer.

    abstract::We evaluated the mechanism of antitumor effects of buserelin, which is one of LH-RH agonists, on a hormone dependent breast cancer model, using 7,12-dimethylbenz(a)anthracene (DMBA)-induced rat mammary cancer. Rats developing solid mammary tumors within 5-7 weeks following the DMBA administration were divided into gro...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Koibuchi Y,Sugamata N,Iino Y,Yokoe T,Andoh T,Maemura M,Takei H,Horiguchi J,Matsumoto H,Morishita Y

    更新日期:1999-08-01 00:00:00

  • Silencing of Rac1 modifies lung cancer cell migration, invasion and actin cytoskeleton rearrangements and enhances chemosensitivity to antitumor drugs.

    abstract::Rac1, an intracellular signal transducer, regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. Overexpression of Rac1 has been reported in several human cancers. However, the underlying mechanisms are not well understood. In the present study, we evaluate...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Chen QY,Xu LQ,Jiao DM,Yao QH,Wang YY,Hu HZ,Wu YQ,Song J,Yan J,Wu LJ

    更新日期:2011-11-01 00:00:00

  • Rosiglitazone attenuates atherosclerosis and increases high-density lipoprotein function in atherosclerotic rabbits.

    abstract::Rosiglitazone has been found to have anti-atherogenic effects and to increase serum high-density lipoprotein (HDL) cholesterol (HDL-C) levels. However, in vivo studies investigating the regulation of adenosine triphosphate-binding cassette transporter A1 (ABCA1) and scavenger receptor class B type I (SR-BI) by rosigli...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Li C,Tu Y,Liu TR,Guo ZG,Xie D,Zhong JK,Fan YZ,Lai WY

    更新日期:2015-03-01 00:00:00

  • Antiproliferative and apoptosis-inducing activity of Brucea javanica extract on human carcinoma cells.

    abstract::We have recently demonstrated the antiproliferative and apoptotic activities of herbal traditional Chinese medicines, including the analomous fruit extract of Gleditsia sinensis, the fresh juice of Scutellaria barbata and the warmed water extract of Radix Sophorae Tonkinensis on a series of human carcinoma cells. Here...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Lau FY,Chui CH,Gambari R,Kok SH,Kan KL,Cheng GY,Wong RS,Teo IT,Cheng CH,Wan TS,Chan AS,Tang JC

    更新日期:2005-12-01 00:00:00

  • Molecular characterization of mesoderm-restricted basic helix-loop-helix protein, POD-1/Capsulin.

    abstract::Members of the basic helix-loop-helix (bHLH) family of transcription factors have been implicated in the regulation of cell proliferation and differentiation. Here we describe molecular characterization of mesoderm-specific bHLH protein, POD-1/Capsulin. Northern blot analysis showed that POD-1/Capsulin are preferentia...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Miyagishi M,Nakajima T,Fukamizu A

    更新日期:2000-01-01 00:00:00

  • Protective effect of Xuebijing injection on D-galactosamine- and lipopolysaccharide-induced acute liver injury in rats through the regulation of p38 MAPK, MMP-9 and HO-1 expression by increasing TIPE2 expression.

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Liu MW,Liu R,Wu HY,Zhang W,Xia J,Dong MN,Yu W,Wang Q,Xie FM,Wang R,Huang YQ,Qian CY

    更新日期:2016-11-01 00:00:00

  • GATA binding protein 3 overexpression and suppression significantly contribute to the regulation of allergic skin inflammation.

    abstract::GATA binding protein 3 (GATA3) is a key molecule regulating the balance in the ratio of type 1 helper T (Th1) cells to type 2 helper T (Th2) cells, which is thought to be indicative of the pathogenesis of allergic diseases such as asthma and atopic dermatitis. The aim of this study was to investigate the role of GATA3...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Bae CJ,Lee JW,Shim SB,Jee SW,Lee SH,Woo JM,Lee CK,Hwang DY

    更新日期:2011-08-01 00:00:00

  • Novel dermo-epidermal equivalents on silk fibroin-based formic acid-crosslinked three-dimensional nonwoven devices with prospective applications in human tissue engineering/regeneration/repair.

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Dal Pra I,Chiarini A,Boschi A,Freddi G,Armato U

    更新日期:2006-08-01 00:00:00

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    abstract::We studied whether feeding pregnant female mice with different fats affects lipid exchange and activity of the splenic lymphoid system in offspring exposed to low doses of carcinogen. Female mice were fed diets with either 7% or 15% corn oil or olive oil. The 4-week-old offspring of these mice were transferred to a ch...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Zusman I,Ben-Hur H,Budovsky A,Geva D,Gurevich P,Tendler Y,Lavee S,Stark A,Madar Z

    更新日期:2000-09-01 00:00:00

  • Broadleaf Mahonia attenuates granulomatous lobular mastitis‑associated inflammation by inhibiting CCL‑5 expression in macrophages.

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Wang Z,Wang N,Liu X,Wang Q,Xu B,Liu P,Zhu H,Chen J,Situ H,Lin Y

    更新日期:2018-01-01 00:00:00

  • Secretogranin III may be an indicator of paraquat-induced astrocyte activation and affects the recruitment of BDNF during this process.

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Zhan X,Li F,Chu Q,Pang H

    更新日期:2018-12-01 00:00:00

  • The influence of angiotensin converting enzyme inhibitors on lipid peroxidation in sera and aorta of rabbits in diet-induced hypercholesterolemia.

    abstract::In hypercholesterolemia increased lipid and lipoprotein peroxidation occurs. The renin-angiotensin system plays an important role in atherogenesis. Angiotensin II induces smooth muscle cells proliferation and stimulates oxidation of LDL particles and foam cell accumulation. Inhibition of ang II production leads to dec...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Wojakowski W,Gminski J,Siemianowicz K,Goss M,Machalski M

    更新日期:2000-11-01 00:00:00

  • MicroRNA expression profiles in rats with selenium deficiency and the possible role of the Wnt/β-catenin signaling pathway in cardiac dysfunction.

    abstract::Selenium deficiency is a causative factor in heart failure and microRNAs (known as miRNAs or miRs) play an important role in numerous cardiovascular diseases. However, the changes of miRNA expression during selenium deficiency and whether selenium deficiency is involved in cardiac dysfunction remain unclear. In the pr...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Xing Y,Liu Z,Yang G,Gao D,Niu X

    更新日期:2015-01-01 00:00:00

  • Triptolide suppresses interleukin-1beta-induced human beta-defensin-2 mRNA expression through inhibition of transcriptional activation of NF-kappaB in A549 cells.

    abstract::The immunosuppressive effect of triptolide has been associated with suppression of T-cell activation. However, the immunosuppressive effects of triptolide on innate immunity in the epithelial barrier remain to be elucidated. Human beta-defensin (HBD)-2 is an inducible antimicrobial peptide and plays an important role ...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Jang BC,Lim KJ,Choi IH,Suh MH,Park JG,Mun KC,Bae JH,Shin DH,Suh SI

    更新日期:2007-05-01 00:00:00

  • The elevation of natural killer cell activity induced by laughter in a crossover designed study.

    abstract::The elevation of natural killer cell activity (NKCA) by laughter was not confirmed due to incomplete methodology of previous studies although positive emotion is believed to be favorable for health. To verify NKCA elevation by laughter in a crossover design, we measured NKCA before and after watching films, presenting...

    journal_title:International journal of molecular medicine

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Takahashi K,Iwase M,Yamashita K,Tatsumoto Y,Ue H,Kuratsune H,Shimizu A,Takeda M

    更新日期:2001-12-01 00:00:00

  • Anti-asthmatic activities in mycelial extract and culture filtrate of Cordyceps sphecocephala J201.

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    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Heo JC,Nam SH,Nam DY,Kim JG,Lee KG,Yeo JH,Yoon CS,Park CH,Lee SH

    更新日期:2010-09-01 00:00:00

  • Immunodetection of the MCHR1 antibody in vitiligo patient sera.

    abstract::Human vitiligo is an acquired depigmenting skin disorder characterized by milk-white skin macules resulting from a chronic and progressive loss of melanocytes. It has been suggested that autoimmune mechanisms are involved in this disorder. We undertook this project to obtain an MCHR1-C linear peptide containing four m...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Zhou M,Guan C,Lin F,Xu W,Fu L,Hong W,Wan Y,Xu A

    更新日期:2011-05-01 00:00:00

  • CpG methylation of the ERalpha and ERbeta genes in breast cancer.

    abstract::Aberrant methylation of promoter CpG islands is known to be responsible for the alteration and silencing of genes in cancer. The data presented here describes that the CpG methylations of ERalpha and ERbeta are found in breast cancer tissues, and methylation exerts a considerable effect on gene silencing, investigated...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Kim SJ,Kim TW,Lee SY,Park SJ,Kim HS,Chung KW,Lee ES,Kang HS

    更新日期:2004-08-01 00:00:00

  • The role of S100A4 gene encoding an S100-related calcium-binding protein in human bile duct adenocarcinoma cell lines: correlation of S100A4 expression and invasive growth in Matrigel Matrix.

    abstract::S100A4, one of the tandemly arranged S100 genes at chromosome 1q21, has been suggested to play a functional role in cell motility and invasiveness of tumor cell growth. We investigated the expression of S100A4 and the in vitro invasiveness of 4 human bile duct adenocarcinoma cell lines by the Matrigel assay. S100A4 wa...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Katayama N,Murao S,Ajiki T,Kitazawa S,Onoyama H,Kuroda Y,Maeda S

    更新日期:2000-11-01 00:00:00

  • Expression of Hu-IFN-alphaR2 chain of type I interferon receptor in human hepatocellular carcinoma and non-cancerous tissues.

    abstract::Type I interferon (IFN) receptor consists of two chains (Hu-IFN-alphaR1 and Hu-IFN-alphaR2), and Hu-IFN-alphaR2 takes a soluble, short, or long form (Hu-IFN-alphaR2a, Hu-IFN-alphaR2b, or Hu-IFN-alphaR2c, respectively). We examined Hu-IFN-alphaR2 expression in hepatocellular carcinoma (HCC) tissues and their correspond...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Takayama A,Yano H,Ogasawara S,Higaki K,Kojiro M

    更新日期:2000-12-01 00:00:00

  • LGR5 is required for the maintenance of spheroid-derived colon cancer stem cells.

    abstract::Colon cancer stem cells (CCSCs) are involved in colon cancer and promote tumor progression and recurrence. LGR5, a marker for intestinal stem cells (ISCs), is also considered to serve as a marker for CCSCs. However, the precise function of LGR5 in CCSCs is unclear. In this study, we demonstrated that LGR5 was highly e...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Chen X,Wei B,Han X,Zheng Z,Huang J,Liu J,Huang Y,Wei H

    更新日期:2014-07-01 00:00:00

  • Cannabinoid receptor type 1 mediates high-fat diet-induced insulin resistance by increasing forkhead box O1 activity in a mouse model of obesity.

    abstract::Hepatic glucose production is promoted by forkhead box O1 (FoxO1) under conditions of insulin resistance. The overactivity of cannabinoid receptor type 1 (CB1R) partly causes increased liver fat deposits and metabolic dysfunction in obese rodents by decreasing mitochondrial function. The aim of the present study was t...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Chen CC,Lee TY,Kwok CF,Hsu YP,Shih KC,Lin YJ,Ho LT

    更新日期:2016-03-01 00:00:00

  • HPV16/18 E5, a promising candidate for cervical cancer vaccines, affects SCPs, cell proliferation and cell cycle, and forms a potential network with E6 and E7.

    abstract::The spindle checkpoint proteins (SCPs), which sense the existence of misaligned sister chromatids during mitosis and meiosis, are essential for cell proliferation and differentiation. Therefore, the role of SCPs in carcinogenesis is gaining increased attention. In this study, we ana...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Liao S,Deng D,Hu X,Wang W,Li L,Li W,Zhou J,Xu G,Meng L,Wang S,Ma D

    更新日期:2013-01-01 00:00:00

  • Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions.

    abstract::Excessive expansion of the transit-amplifying (TA) cell compartment is a distinct morphological characteristic of psoriatic epidermal hyperplasia. In order to examine the activation of basal stem cells and how they replenish such an enlarged compartment of TA cells in psoriatic epidermis, we utilized a BrdU labeling m...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Jia HY,Shi Y,Luo LF,Jiang G,Zhou Q,Xu SZ,Lei TC

    更新日期:2016-02-01 00:00:00

  • Beacon[47-73] inhibits glucocorticoid secretion and growth of cultured rat and human adrenocortical cells.

    abstract::Evidence has been recently provided that beacon, an ubiquitin-like protein overexpressed in the hypothalamus of Israeli sand rat, is also expressed in several endocrine glands of the Wistar rat, including adrenal cortex. Moreover, it has been shown that the in vivo administration of beacon[47-73] (hereinafter, beacon)...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Ziolkowska A,Carraro G,Rebuffat P,Spinazzi R,Nussdorfer GG,Rucinski M,Malendowicz LK

    更新日期:2004-09-01 00:00:00

  • Epidermal growth factor downregulates the expression of SH-PTP2.

    abstract::Protein phosphorylation/dephosphorylation on tyrosine residues are regulated by tyrosine kinases/phosphatases. The tyrosine phosphatase SH-PTP2 (PTP1D, PTP2C) interacts physically through its SH2 domain with phosphorylated epidermal growth factor receptor (EGFR). In KB cells, an oral epidermoid carcinoma, high epiderm...

    journal_title:International journal of molecular medicine

    pub_type: 杂志文章


    authors: Kamer AR,Hoghooghi SA,Liebow C

    更新日期:1998-04-01 00:00:00