Abstract:
:Macrophage-like tumor cells can be obtained in large quantities as rather homogeneous populations, making these cells useful for chemotaxis assays. Therefore, macrophage-like cells J774A, WEHI-3, P388D1, IC-21, and NCTC 1469, all of murine origin, and U937 of human origin, were tested for chemotactic activity to a number of chemoattractive agents, such as casein, an N-formyl tetrapeptide (N-formyl-L-norleucyl-L-leucyl-L-phenylalanyl-L-tyrosine), and culture supernatants of murine SL2 lymphoma cells. J774A and WEHI-3 macrophage-like cells of murine (BALB/c) origin expressed the strongest chemotactic activity to casein and N-formyl tetrapeptide, respectively. The results show that: very standardized chemotaxis assays can be performed using these cell lines; these assays require appropriate cell line-stimulus combinations; there are substantial differences among cell lines as to sensitivity to various chemoattractive substances; macrophage cell lines and functional mutants may be helpful for the study of receptors for chemotaxins and the study of transducer signals for chemotaxis.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Terheggen P,Van Loveren H,Den Otter Wdoi
10.1002/jlb.38.6.747subject
Has Abstractpub_date
1985-12-01 00:00:00pages
747-52issue
6eissn
0741-5400issn
1938-3673journal_volume
38pub_type
杂志文章abstract::Eosinophils are traditionally associated with allergic and parasitic inflammation. More recently, eosinophils have also been shown to have roles in diverse processes including development, intestinal health, thymic selection, and B-cell survival with the majority of these insights being derived from murine models and ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.3HR0220-396R
更新日期:2020-07-01 00:00:00
abstract::Macrophage activation by IFN-gamma results in a cascade of gene expression. To identify genes activated in mouse peritoneal macrophages by IFN-gamma, we created a cDNA subtraction library of IFN-gamma-induced genes. We have isolated from this subtraction library a novel cDNA clone, called Mg21, whose mRNA is absent in...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.57.3.477
更新日期:1995-03-01 00:00:00
abstract::Seventy-kilodalton amino-terminal and 180-kDa cell-binding fibronectin fragments were used to determine which fibronectin domains support soluble gelatin interactions with macrophages. At each time measured, intact and 180-kDa fibronectin supported significantly larger quantities of cell-associated gelatin than contro...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.58.5.501
更新日期:1995-11-01 00:00:00
abstract::Matrix metalloproteinases-2 and -9 (MMP-2/9) are critically involved in degradation of extracellular matrix, and their inhibition is discussed as a promising strategy against hepatic ischemia-reperfusion (I/R) injury. Here, we analyzed the role of MMP-2 and -9 for leukocyte migration and tissue injury in sham-operated...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0805468
更新日期:2006-06-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is a multifunctional cytokine that plays a pivotal role in mediating neovascularization as well as other endothelial cell alterations during inflammation. In this study, we demonstrate that human neutrophils are a source of VEGF. We observed that isolated blood neutrophils rel...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.62.3.397
更新日期:1997-09-01 00:00:00
abstract::Postnatal stem cells regulate the homeostasis of the majority of our tissues. They continuously generate new progenitors and mature, functional cells to replace old cells, which cannot assume the tissue function anymore and are eliminated. Blood, skin, gut mucosa, muscle, cartilage, nerves, cornea, retina, liver, and ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0505272
更新日期:2005-10-01 00:00:00
abstract::Mice with a naturally occurring Csfm(op)/Csfm(op) (op/op) gene mutation lack functional macrophage-colony stimulating factor (M-CSF) and are deficient of M-CSF-derived macrophages. They are severely monocytopenic, and their remaining M-CSF-independent macrophages were shown to differ in differentiation and distinct fu...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.12011003
更新日期:2003-05-01 00:00:00
abstract::SHP-1 is a cytoplasm protein tyrosine phosphatase expressed primarily in hematopoietic cells. In the immune system, SHP-1 plays critical roles in regulation of many receptor-mediated signaling cascades, and SHP-1 deficiency in mice causes spontaneous inflammation and autoimmunity. Here, we report a unique requirement ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0409289
更新日期:2010-05-01 00:00:00
abstract::As the natural resistance-associated macrophage protein 1 Nramp1 (also known as Slc11a1) modulates Kupffer cell (KC) activation, and KC are responsible for the early phase of warm ischemia/reperfusion (I/R) to the liver, we hypothesized that livers of Nramp1(-/-) mice will be protected from early-phase I/R injury comp...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::We report the systematic use of large-scale cDNA microarrays to study the gene expression profiles of primary human peripheral blood monocytes (MONO) in comparison with in vitro-differentiated, M-CSF-induced MONO-derived macrophages (MAC) and primary human alveolar MAC (AM), obtained by bronchoalveolar lavage from the...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0206124
更新日期:2007-01-01 00:00:00
abstract::During highly active antiretroviral therapy (HAART), HIV-1 can still persist in circulating, resting CD4+ T lymphocytes, lymph node mononuclear cells, and seminal cells of patients despite sustained suppression of plasma viremia to undetectable levels. Sanctuary sites where antiretroviral drug penetration is not optim...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:
更新日期:2000-09-01 00:00:00
abstract::Mucosa represents the main site of pathogen/cell interactions. The two main types of cells forming the epithelial structure [epithelial cells and Langerhans cells (LC)] coordinate the first defense responses to avoid infection. To evaluate the involvement of epithelial cells in the early steps leading to a specific ad...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0305147
更新日期:2005-07-01 00:00:00
abstract::Virulent Legionella pneumophila replicate readily in thioglycollate-elicited peritoneal macrophages from genetically permissive A/J mice, but avirulent L. pneumophila do not. The production of cytokines by macrophages infected with L. pneumophila has been studied, but the correlation of bacterial virulence with immune...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.67.6.863
更新日期:2000-06-01 00:00:00
abstract::Although mouse models of inflammatory skin diseases such as psoriasis and atopic dermatitis fail to completely phenocopy disease in humans, they provide invaluable tools to examine the molecular and cellular mechanisms responsible for the epidermal hyperplasia, inflammation, and excess angiogenesis observed in human d...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0607347
更新日期:2008-07-01 00:00:00
abstract::Glucocorticoids, such as dexamethasone (Dex), are used clinically in the treatment of various inflammatory diseases. Dex acts by inhibiting the expression of inflammatory mediators, such as tumor necrosis factor alpha (TNF-alpha) and monocyte chemoattractant protein-1 (MCP-1). It is surprising that Dex enhances bacter...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0204068
更新日期:2004-07-01 00:00:00
abstract::We reported previously that N-myc downstream-regulated gene 2 (NDRG2), a member of a new family of differentiation-related genes, is expressed specifically in dendritic cells (DC) differentiated from monocytes, CD34(+) progenitor cells, and the myelomonocytic leukemic cell line. In this study, we demonstrate that NDRG...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0507300
更新日期:2008-01-01 00:00:00
abstract::The objective of this study was to find the role of LncRNA SNHG12 in the regulation of hypertensive vascular endothelial injury. LncRNA SNHG12 and miR-25-3p expression were detected by quantitative RT-PCR. Protein levels of Sirtuin 6 (SIRT6), endothelial cell (EC) senescence markers p16 and p21, and EC marker CD31 wer...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1A0820-501R
更新日期:2021-01-19 00:00:00
abstract::An accessory cell line, designated line A, was generated from bone marrow stem cells which differentiated in vitro in response to colony-stimulating factor (CSF) in substratum cultures. The cells were found to constitutively secrete large amounts of CSF, the activity of which was neutralized by anti-CSF-1 antibodies. ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.40.2.183
更新日期:1986-08-01 00:00:00
abstract::On the surface of the macrophage, annexin A2 tetramer (A2t) serves as a docking protein or recognition element for bacterial and viral pathogens. Plasma levels of free A2t have been reported to increase following infection, although the mechanistic significance of this observation is unclear. Although annexin A2 had g...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0307154
更新日期:2007-11-01 00:00:00
abstract::STAT1 is a key effector of cytokines involved in the resistance to pathogens; its identified transcriptional targets mediate the innate immune response involved in the defense against viruses and bacteria. Little is known about the role of STAT1 in adaptive immunity, including its impact on BCR or surface Ig expressio...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1109714
更新日期:2010-06-01 00:00:00
abstract::Polymorphonuclear leukocyte (PMN) respiratory burst was stimulated by heterologous antibodies against PMN granule proteins but not by control antibodies. Fluorescence-activated cell sorter (FACS) analysis of activated PMN demonstrated the presence of two primary granule proteins, proteinase 3 (PR-3) and cationic prote...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.50.6.539
更新日期:1991-12-01 00:00:00
abstract::In recent years, it has become appreciated that immune cells have different metabolic profiles depending on their activation status. During sepsis, circulating leukocytes go through a hyperinflammatory state, which can be accompanied or followed by defective antimicrobial defenses (also described as immune tolerance o...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.4MR0216-066R
更新日期:2017-01-01 00:00:00
abstract::In the absence of IL-10, colonic inflammation ensues, which is characterized by high levels of IL-17. Here, we demonstrate a direct correlation between ICOS expression and IL-17 production in cIELs. IL-10(-/-) mice had increased numbers of cIELs and greater colon weight. Although the CD69 early activation antigen was ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0409238
更新日期:2010-02-01 00:00:00
abstract::The key role of TRAF6 in TLR signaling pathways is well known. More recent evidence has implicated TRAF3 as another TRAF family member important to certain TLR responses of myeloid cells. Previous studies demonstrate that TRAF3 functions are highly context-dependent, displaying receptor and cell-type specificity. We t...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0111044
更新日期:2011-12-01 00:00:00
abstract::TLRs are a class of pattern recognition receptors (PRRs) that detect invading microbes by recognizing pathogen-associated molecular patterns (PAMPs). Upon PAMP engagement, TLRs activate a signaling cascade that leads to the production of inflammatory mediators. The localization of TLRs, either on the plasma membrane o...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1002/JLB.3MIR1218-483RR
更新日期:2019-07-01 00:00:00
abstract::We studied the tissue distribution of CD6(+) lymphocytes and cells expressing the CD6 ligand (also known as activated leukocyte cell adhesion molecule CD166) in calves by immunohistochemistry using an anti-bovine CD6 monoclonal antibody (mAb), a human CD6 (huCD6)-immunoglobulin G1 fusion protein (huCD6-Ig), and an ant...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::The human lysozyme (LZM) gene is highly methylated in LZM-nonexpressor immature myeloid and in nonmyeloid cells and unmethylated only in LZM-expressing cells. Extended methylation analyses of the CpG-poor 5' flanking region and of the exon 4 CpG island (both containing Alu elements) of the LZM gene were now performed....
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0106005
更新日期:2006-12-01 00:00:00
abstract::Mycoplasmas and mycoplasma membranes have been shown to induce the production of inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, as well as nitric oxide, by mouse macrophages and rat brain astrocytes. Luminol-enhanced chemiluminescence was used as a sensitive method to show that Mycopl...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.57.2.264
更新日期:1995-02-01 00:00:00
abstract::CD8(+) T cells must detect foreign antigens and differentiate into effector cells to eliminate infections. But, when self-antigen is recognized instead, mechanisms of peripheral tolerance prevent acquisition of effector function to avoid autoimmunity. These distinct responses are influenced by inflammatory and regulat...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1A0913-500RR
更新日期:2014-09-01 00:00:00
abstract::MMPs are generally considered to regulate degradation and remodeling of the ECM. Convincing data also implicate a role for MMPs in inflammatory conditions, such as AP, although the mechanisms are not known. The aim of this study was to define the role of MMPs in regulating activation of trypsinogen and tissue damage i...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0811443
更新日期:2012-05-01 00:00:00