Abstract：
AIMS:The role of neutrophils in diabetes and its complications is unclear. Upon challenge with microbes and inflammatory triggers, neutrophils release enzymes and nuclear material, forming neutrophils extracellular traps (NETs) and thereby dying by NETosis. We herein tested NET formation and NETosis products in high glucose and in the setting of type 2 diabetes (T2D). METHODS:NETosis was assessed in vitro in cells exposed to 0, 5, 25 mM glucose and 25 mM mannitol, DMSO and PMA using immunofluorescence staining for elastase, DNA and chromatin. Single-cell morphometric analysis was used to detect enter of elastase in the nucleus and extrusion of nuclear material. Release of NETs was quantified by staining with Hoechst 33342. In 38 T2D and 38 age- and sex-matched non-diabetic individuals, we determined plasma elastase, mono- and oligonucleosomes and double-strand (ds) DNA, as circulating NETosis products. RESULTS:NETosis was accurately reproduced in vitro: high (25 mM) glucose increased NETosis rate and release of NETs compared with 5 mM glucose and 25 mM mannitol. T2D patients showed increased plasma elastase, mono- and oligonucleosomes and dsDNA compared with non-diabetic control individuals. A positive correlation was found between HbA1c and mono- and oligonucleosomes, whereas dsDNA was correlated with the presence of nephropathy and cardiovascular disease. Serum IL-6 concentrations were higher in T2D compared with CTRL and correlated with serum dsDNA levels. CONCLUSIONS:High glucose and hyperglycemia increase release of NETs and circulating markers of NETosis, respectively. This finding provides a link among neutrophils, inflammation and tissue damage in diabetes.
journal_name
Acta Diabetoljournal_title
Acta diabetologicaauthors
Menegazzo L,Ciciliot S,Poncina N,Mazzucato M,Persano M,Bonora B,Albiero M,Vigili de Kreutzenberg S,Avogaro A,Fadini GPdoi
10.1007/s00592-014-0676-xsubject
Has Abstractpub_date
2015-06-01 00:00:00pages
497-503issue
3eissn
0940-5429issn
1432-5233journal_volume
52pub_type
杂志文章abstract：:Cardiovascular risk is determined by multiple risk factors, all of which greatly increase the chance of morbidity and mortality. So-called "normal" levels of these factors are not biologically normal, so current strategy is based on estimations of a person's global cardiovascular risk, and then using appropriate combi...
journal_title：Acta diabetologica
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00592-005-0181-3
更新日期：2005-04-01 00:00:00
abstract：:Cardiovascular diseases represent, today, the principal cause of mortality in the general population, especially in subjects with type 2 diabetes mellitus. In these patients the risk of death from cardiovascular diseases is equal to that of non-diabetic subjects with a previous episode of myocardial infarction. Many f...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-003-0121-z
更新日期：2003-12-01 00:00:00
abstract：:Hemoglobin A1c (HbA1c) has been recommended as an optional method for diagnosing diabetes. The impact of HbA1c on the diagnosis of diabetes has not been evaluated in China, a country with the greatest number of people with diabetes in the world. Hence, we aim to examine how well HbA1c performs as compared with fasting...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-013-0515-5
更新日期：2014-01-01 00:00:00
abstract：AIM:It is unclear whether any breastfeeding or a certain duration of breastfeeding is protective against the development of diabetes in adult offspring. METHODS:We followed a sub-sample of 3,595 offspring born in the Mater Hospital in Brisbane, Australia between 1981 and 1983 and for whom we had doctor diagnosed self-...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0690-z
更新日期：2015-10-01 00:00:00
abstract：:The proinflammatory lysophosphatidic acid (LPA) is a potent activator of several transcriptional factors and signaling pathways and a potent modulator of genes involved in inflammation, angiogenesis and fibrosis. This study was conducted to measure the levels of LPA and LPA-producing enzymes, autotaxin (ATX) and acylg...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-012-0422-1
更新日期：2013-06-01 00:00:00
abstract：:MicroRNAs (miRNAs) play a crucial role in the pathogenesis of type 2 diabetes (T2D); they regulate several metabolic pathways including insulin secretion, glucose homeostasis, so their potential as biomarkers of diagnosis and prognosis has became increasingly appreciated. In this study, we explore serum miRNA profiles...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0617-8
更新日期：2014-10-01 00:00:00
abstract：AIM:Infection of a diabetic foot ulcer (DFU) is common. More than the half of DFUs become infected and 15-20% of them necessitate amputation in course of treatment. Diabetic foot infection (DFI) is therefore the major cause for non-traumatic lower limb amputation in Germany. Prompt and effective treatment of DFI is man...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-020-01587-5
更新日期：2020-09-17 00:00:00
abstract：:Pancreatic volume and fat content might be associated with β-cell function or insulin resistance (IR). We investigated the difference in pancreatic volume and fat content between age- and body mass index (BMI)-matched normal subjects and patients with having different durations of type 2 diabetes (T2D). We compared pa...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0581-3
更新日期：2014-10-01 00:00:00
abstract：AIMS:The main objective of this study was to estimate the incidence rate and prevalence of pediatric type 1 diabetes mellitus (T1DM; population 0-18 years of age) in the northeastern Italian region Friuli Venezia Giulia and to characterize the subjects affected by the disease. METHODS:This was a retrospective populati...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-016-0854-0
更新日期：2016-08-01 00:00:00
abstract：:The aim of this study was to investigate the influence of homocysteine (hcy) levels on endothelial function by the method of brachial artery ultrasonography and their relation with microvascular complications in type 2 diabetes mellitus (T2DM) patients without macrovascular disease. Fifty-nine T2DM patients with a mea...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0245-7
更新日期：2007-06-01 00:00:00
abstract：:To define whether reference values for bioimpedance analysis (BIA) can be predicted in healthy individuals, individual characteristics and BIA variables (resistance index=height(2)/parallel resistance and reactance index= height(2)/parallel reactance) were evaluated in non-obese healthy individuals: 863 men and 769 wo...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-003-0073-3
更新日期：2003-10-01 00:00:00
abstract：:We analysed the risk factors associated with diabetic complications in the cohort of patients assisted by a type 2 diabetes mellitus (T2DM) shared-care program. We analysed registry data from 16,136 T2DM patients. Of them, 4,781 had microangiopathy, 3,469 CV events. They were 70.5 ± 17.1 years old, 50% were male, dise...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-010-0186-4
更新日期：2010-12-01 00:00:00
abstract：:Elevated plasma CT-pro-vasopressin (copeptin) has been described as biomarkers for type 2 diabetes (T2D) and the metabolic syndrome (MetS), which, however, was not confirmed by all studies. Here, we analyzed the association of copeptin with T2D, MetS and MetS components in the population-based KORA F4 study. Plasma co...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0609-8
更新日期：2015-02-01 00:00:00
abstract：:The recent efforts in the study of fetal compartments could help us to a better recognition of the proper fetal growth and well being. Fat content correlates directly with energy stores. Fat mass and lean body mass is often used in the nutritional assessment of the individual. Moreover chromosomal abnormalities may be...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-003-0033-y
更新日期：2003-10-01 00:00:00
abstract：AIMS:Observational studies indicated that resting heart rate (RHR) was associated with diabetes mellitus (DM) risk; however, it remains unclear whether the association between RHR and DM is causal. We aimed to examine whether there was causal association of RHR with DM risk. METHODS:A prospective study including 16,20...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-019-01344-3
更新日期：2019-09-01 00:00:00
abstract：:Patients with diabetes represent an increasing proportion of end-stage renal disease (ESRD) patients. Cardiovascular risk, already formidable among patients on dialysis, is significantly higher among those who also have diabetes. Diabetic ESRD patients are not only at higher risk of ischaemic events, but are also subj...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s005920200003
更新日期：2002-04-01 00:00:00
abstract：:To evaluate the role of insulin resistance and beta cell function to increasing fasting plasma glucose (FPG), 1,272 Chinese subjects (18-80 years of age) were divided into normal glucose tolerance (NGT), impaired fasting glucose (IFG), impaired glucose tolerance (IGT), combined glucose intolerance (CGI), and type 2 di...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-010-0194-4
更新日期：2012-12-01 00:00:00
abstract：AIMS:Familial partial lipodystrophy (FPLD) is a rare autosomal dominant disorder, mostly due to mutations in lamin A (LMNA) or in peroxisome proliferator-activated receptor gamma (PPARG) genes. In the present study, we aimed to identify and functionally characterize the genetic defect underlying FPLD in an Italian fami...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-019-01462-y
更新日期：2020-05-01 00:00:00
abstract：:The aim was to investigate the long-term incidence of proliferative diabetic retinopathy (PDR), and progression and regression of diabetic retinopathy (DR) and associated risk factors in young Danish patients with Type 1 diabetes mellitus. In 1987-89, a pediatric cohort involving approximately 75 % of all children wit...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-013-0527-1
更新日期：2014-01-01 00:00:00
abstract：:The aim of the study was to report the prevalence of obesity indices in individuals with diabetes and find out their association with diabetic retinopathy in the urban Indian population. Subjects (n = 1,414) were recruited from Sankara Nethralaya Diabetic Retinopathy Epidemiology And Molecular Genetics Study (SN-DREAM...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-009-0113-8
更新日期：2010-09-01 00:00:00
abstract：:The relationship between insulin responses to glucose and to arginine was studied in non-obese women with previous gestational diabetes (PGD). One group, n = 10, had normal glucose tolerance (NGT) by WHO criteria and another, n = 8, had impaired glucose tolerance (IGT). A third group of women without PGD, n = 12, was ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00569563
更新日期：1995-06-01 00:00:00
abstract：AIMS:Although diabetes is rapidly increasing in India, there is no national consensus on best practices for screening, diagnosis, and management of gestational diabetes mellitus (GDM). The goal of this study was to systematically review the literature for studies reporting the prevalence and screening and diagnostic me...
journal_title：Acta diabetologica
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00592-018-1131-1
更新日期：2018-06-01 00:00:00
abstract：:A literature review of 37 papers cited by MEDLINE between 1986 and April 1996 under the terms "diabetes", "patient education" and "randomized" was carried out. The articles were analysed on the basis of a check list (Educational Procedure Check List), which contains 27 items grouped into 8 areas: study objectives, edu...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s005920050133
更新日期：1998-12-01 00:00:00
abstract：:Comparing macrophage-derived cytokine and nitric oxide (NO) profiles in type I and type II diabetes mellitus (DM); and determining whether thymoquinone (TQ) has any modulatory effect were the main objectives of the present study. Peritoneal macrophages have been collected from Otsuka Long-Evans Tokushima Fatty (OLETF)...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-005-0170-6
更新日期：2005-03-01 00:00:00
abstract：AIMS:The anti-inflammatory effects of the polyphenol resveratrol in patients with type 2 diabetes mellitus (T2DM) are controversial. Its role on pentraxin 3 (PTX3) concentrations, a human acute phase protein, has never been evaluated. Our aim was to determine whether a two-dosage resveratrol supplementation (500 and 40...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-017-0977-y
更新日期：2017-05-01 00:00:00
abstract：AIMS:This study aimed to assess islet insulin secretion and insulin resistance in Chinese patients with latent autoimmune diabetes in adults (LADA). METHODS:Twelve patients with LADA, 10 with type 1 diabetes mellitus (T1DM), 10 with type 2 diabetes mellitus (T2DM), and 10 nondiabetic healthy controls (HCs) were includ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-019-01387-6
更新日期：2019-11-01 00:00:00
abstract：AIMS:Hypoglycaemia represents a critical burden with clinical and social consequences in the management of diabetes. Serum uric acid (SUA) has been associated with cardiovascular diseases (CVD), but no conclusive findings are available nowadays in patients suffering from hypoglycaemia. We investigated whether SUA level...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-017-1094-7
更新日期：2018-04-01 00:00:00
abstract：:We have measured protein phosphatase (PP) activity in crude homogenates as well as in the total 105.000 x g supernatant and precipitate fractions from normal rat pancreatic islets. On the basis of the inhibition produced by either 1 nM or 1 microM okadaic acid, both PP1 and PP2A activity were present in crude islet ho...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s005920050057
更新日期：1997-03-01 00:00:00
abstract：:Despite advances in technology, the frequent self-measurement of blood glucose (SMBG) remains fundamental to the management of 1 diabetes mellitus (T1DM). Once measured, SMBG results are routinely reported back to health professionals and other interested parties, either verbally, via a logbook, or electronically down...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-016-0907-4
更新日期：2017-01-01 00:00:00
abstract：:Diabetic neuropathy is a significant complication of chronic diabetes. We wished to discover whether we were successfully identifying patients who had developed neuropathic problems. Specifically, Diabetologists are traditionally poor at asking about erectile dysfunction (ED) and patients themselves are not always for...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-008-0084-1
更新日期：2009-12-01 00:00:00
