Ficoll fractionation for the separation of parasitized erythrocytes from malaria infected blood.

Abstract:

:Separation and concentration of parasitized erythrocytes from infected blood was achieved by centrifugation of a sample placed in a layer on top of a cushion of a Ficoll solution with a critical density. Pure suspensions of parasitized erythrocytes were obtained from Plasmodium berghei infected rodent blood, whereas results with P. vivax infected monkey (Aotus trivirgatus) blood were partially successful. Titration experiments revealed that the parasitized erythrocytes obtained by Ficoll fractionation were infective.

authors

Eling W

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

105-14

issue

1

eissn

0042-9686

issn

1564-0604

journal_volume

55

pub_type

杂志文章
  • Survey of BCG vaccination policy in Europe: 1994-96.

    abstract::A retrospective survey, based on a standardized questionnaire sent to qualified public health experts in tuberculosis in 50 European countries, was carried out to evaluate the following: concordance between national vaccination programmes and WHO recommendations on BCG vaccination for prevention of tuberculosis; relat...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Trnka L,Dankova D,Zitova J,Cimprichova L,Migliori GB,Clancy L,Zellweger JP

    更新日期:1998-01-01 00:00:00

  • A phase I clinical trial of mefloquine in Brazilian male subjects.

    abstract::A double-blind, randomized phase I clinical trial was carried out to compare mefloquine with sulfadoxine-pyrimethamine for safety and tolerance. Twenty adult male Brazilian subjects from areas endemic for malaria were studied for a period of 66 days, which included 2 days of basal studies and a 63-day follow-up after ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: de Souza JM

    更新日期:1983-01-01 00:00:00

  • Questionnaires for rapid screening of schistosomiasis in sub-Saharan Africa.

    abstract::New initiatives are aiming to reduce the global burden of schistosomiasis, mainly through the large-scale application of chemotherapy. To target chemotherapy effectively, rapid assessment procedures are needed for identifying high-risk communities that are foci for the disease. In this review, we examine the developme...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Lengeler C,Utzinger J,Tanner M

    更新日期:2002-01-01 00:00:00

  • Physical growth of children and adolescents in China over the past 35 years.

    abstract:OBJECTIVE:To examine if economic development in China correlates with physical growth among Chinese children and adolescents. METHODS:The height, body weight and physical activity level of children and adolescents aged 18 years and under, as well as dietary data, were obtained from seven large surveys conducted in Chi...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.13.126243

    authors: Zong XN,Li H

    更新日期:2014-08-01 00:00:00

  • Improving antibiotic prescribing in Hai Phong Province, Viet Nam: the "antibiotic-dose" indicator.

    abstract:OBJECTIVE:To improve the use and dosage of antibiotics prescribed at Commune Health Stations in Viet Nam, and in so doing find out whether antibiotic dosage can be easily and reliably measured as a drug-use indicator. METHODS:All commune health workers from the 217 commune health stations in Hai Phong Province, Viet N...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Chalker J

    更新日期:2001-01-01 00:00:00

  • Resistant and sensitive strains of Mycobacterium tuberculosis found in repeated surveys among a South Indian rural population.

    abstract::The findings in a highly selected group of patients, such as those attending clinics or sanatoria, cannot be used as the basis for assessing the true prevalence of strains of Mycobacterium tuberculosis with acquired or primary resistance or of sensitive strains in a community. The present report describes the prevalen...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Narain R,Chandrasekhar P,Satyanarayanachar RA,Lal P

    更新日期:1968-01-01 00:00:00

  • Effects of storage and transport on the cultivability of mycobacteria.

    abstract::In the course of WHO/UNICEF-assisted tuberculosis surveys carried out in a number of African territories, sputa were microscopically examined for the presence of acid-fast bacilli. Since adequate facilities for performing cultures to confirm the diagnosis of tuberculosis and other mycobacterial infections were not ava...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Sula L,Sundaresan TK,Langerová M

    更新日期:1960-01-01 00:00:00

  • A comparison of cardiovascular measurements in the Gambia, Jamaica, and the United Republic of Tanzania.

    abstract::Epidemiological studies of the cardiovascular characteristics of three typically rural communities in the Gambia, Jamaica, and the United Republic of Tanzania were carried out by means of standardized methodology. This paper reports comparisons of arterial blood pressure distribution and electrocardiographic findings ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Vaughan JP,Miall WE

    更新日期:1979-01-01 00:00:00

  • A specific ELISA method for determining chloroquine in urine or dried blood spots.

    abstract::Reported is an enzyme-linked immunosorbent assay (ELISA) that has been optimized and validated for the determination of chloroquine in urine or dried blood spots. The assay employs antisera raised in sheep to a chloroquine derivative conjugated to keyhole limpet haemocyanin and chloroquine conjugated to porcine thyrog...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Rowell V,Rowell FJ,Baker A,Laurie D,Sidki AM

    更新日期:1988-01-01 00:00:00

  • Taxonomic value of spermatheca size for distinguishing four members of the Anopheles gambiae complex in East Africa.

    abstract::Measurements of spermatheca diameter and wing length were made on 8 East African mosquito populations comprising Anopheles merus and A. gambiae species A, species B, and the "Bwamba cytotype" (=species D). The results showed that, on average, the spermatheca in species A females is larger than in species B. In each of...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: White GB,Muniss JN

    更新日期:1972-01-01 00:00:00

  • Antibiotic-resistant strains of E1 Tor vibrio in the Philippines and the use of furalazine for chemotherapy.

    abstract::Among 1109 patients with bacteriologically confirmed El Tor cholera admitted to the San Lazaro Hospital, Manila, in 1969, 11 patients continued to excrete vibrios of the same biotype and serotype in stools for more than 1 week in spite of antibiotic treatment.The strains isolated from these patients all belonged to th...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Kobari K,Takakura I,Nakatomi M,Sogame S,Uylangco C

    更新日期:1970-01-01 00:00:00

  • In vitro cultivation of the rickettsial agent of trench fever.

    abstract::Although trench fever appears to be endemic in many areas of the world, recognition of the disease has been handicapped by the difficulties of making a clinical diagnosis and the unavailability of a simple laboratory procedure to establish the etiology. The author describes a method for the in vitro cultivation of Ric...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Vinson JW

    更新日期:1966-01-01 00:00:00

  • Asbestos: use, bans and disease burden in Europe.

    abstract:OBJECTIVE:To analyse national data on asbestos use and related diseases in the European Region of the World Health Organization (WHO). METHODS:For each of the 53 countries, per capita asbestos use (kg/capita/year) and age-adjusted mortality rates (deaths/million persons/year) due to mesothelioma and asbestosis were ca...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:10.2471/BLT.13.132118

    authors: Kameda T,Takahashi K,Kim R,Jiang Y,Movahed M,Park EK,Rantanen J

    更新日期:2014-11-01 00:00:00

  • Use of insecticide-impregnated bed nets in Gambian primary health care: economic aspects.

    abstract::Village-wide use of permethrin-impregnated bed nets, compared with placebo-treated nets, has reduced clinical attacks of malaria by 63% in the Gambia. Costs were calculated for nets made by local tailors and for their treatment with insecticide in the villages, as well as for targeted chemoprophylaxis and back-up trea...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: MacCormack CP,Snow RW,Greenwood BM

    更新日期:1989-01-01 00:00:00

  • Clinical observations on treatment of alkylmercury poisoning in hospital patients.

    abstract::Twenty-six patients suffering from methylmercury poisoning were treated by different therapeutic regimes. Seven received penicillamine or N-acetyl-d-L-penicillamine, 10 were treated with dimercaprol sulfonate and 9, who could not be treated, were given a placebo and were used as controls. Penicillamine, N-acetyl-d-L-p...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Bakir F,Al-Khalidi A,Clarkson TW,Greenwood R

    更新日期:1976-01-01 00:00:00

  • The international reference preparation of colistin methane sulfonate.

    abstract::The international unit of colistin methane sulfonate has been defined by collaborative assay as the activity contained in 0.00007874 mg of the international reference preparation. The definition was based on results from 7 laboratories in 5 countries which carried out assays against their existing national standards. ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Lightbown JW,Bond JM,Grab B

    更新日期:1973-01-01 00:00:00

  • The efficacy of live influenza vaccines.

    abstract::Administration of live influenza vaccine produces an asymptomatic immunizing infection of the respiratory tract with the appearance of antibodies in the secretions of the respiratory passages.Because of natural variation in the antigenic and biological properties of the influenza virus, new vaccines must be developed ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Smorodincev AA

    更新日期:1969-01-01 00:00:00

  • Approaches to control sexually transmitted diseases in Haiti, 1992-95.

    abstract::Despite major obstacles, activities to control sexually transmitted diseases (STDs) were initiated in Haiti in 1992 in collaboration with local nongovernmental organizations. The approaches included review of available local data, assessment of STD case management practices and constraints, and development of specific...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Behets FM,Génécé E,Narcisse M,Liautaud B,Cohen M,Dallabetta GA

    更新日期:1998-01-01 00:00:00

  • A controlled field trial of the typhoid component of polyvalent enteric vaccine (NIISI polyvaccine).

    abstract::At the request of the Ministry of Health of the USSR, a controlled field trial of a polyvaccine containing typhoid, paratyphoid and dysentery antigens and a purified tetanus toxoid was undertaken in 1958. The main object of this trial, which was carried out over a 10-month period simultaneously in four localities, was...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: KHASANOV MI,KHEIFETS LB,SALMIN LV

    更新日期:1962-01-01 00:00:00

  • Prevention of blindness and priorities for the future.

    abstract::The impact of visual loss has profound implications for the person affected and society as a whole. The majority of blind people live in developing countries, and generally, their blindness could have been avoided or cured. Given the current predictions that the number of blind people worldwide will roughly double by ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章,评审

    doi:

    authors: West S,Sommer A

    更新日期:2001-01-01 00:00:00

  • Feasibility study of community control programmes for cystic fibrosis: memorandum from a WHO/ICF(M)A meeting.

    abstract::As a result of improved medical services and diagnosis of cystic fibrosis (CF), it has in recent years become clear that this is a common genetic disorder with a worldwide distribution. The average life expectancy of CF patients is very low in the developing world, but in developed countries this fatal childhood disea...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors:

    更新日期:1990-01-01 00:00:00

  • Rapid, low-cost, two-step method to screen for urinary schistosomiasis at the district level: the Kilosa experience.

    abstract::The operational and diagnostic performance of a two-step method for the cost-effective screening of urinary schistosomiasis was investigated in the Kilosa District of east-central Tanzania. In the first step a simple questionnaire was administered to 15,073 primary schoolchildren by their class teachers over a 4-week ...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Lengeler C,Kilima P,Mshinda H,Morona D,Hatz C,Tanner M

    更新日期:1991-01-01 00:00:00

  • The urban environment and health in a world of increasing globalization: issues for developing countries.

    abstract::Urban living is the keystone of modern human ecology. Cities have multiplied and expanded rapidly worldwide over the past two centuries. Cities are sources of creativity and technology, and they are the engines for economic growth. However, they are also sources of poverty, inequality, and health hazards from the envi...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章,评审

    doi:

    authors: McMichael AJ

    更新日期:2000-01-01 00:00:00

  • Immunity to cancer: naturally occurring tumours in domestic animals as models for research. 2.

    abstract::Part 1 of this Memorandum appeared in Vol. 49, pages 81-91. Part 2 covers neoplasia of the bovine urinary bladder; tumours of unknown cause (including melanomas, osteosarcomas, mammary tumours, lymphosarcomas, mastocytomas, transmissible venereal tumours, and histiocytomas); and the applications of animal models in st...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors:

    更新日期:1973-01-01 00:00:00

  • Rodenticides in bubonic-plague control.

    abstract::The relatively recent development of more-effective pulicides has been paralleled by the discovery of more-efficient rodenticides. Eight of the latter-namely, warfarin, pival, red squill, alpha-naphthylthiourea (ANTU), zinc phosphide, arsenic trioxide, thallium sulfate, and sodium fluoroacetate (1080)-are discussed wi...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: LINK VB,MOHR CO

    更新日期:1953-01-01 00:00:00

  • Bilharziasis in Swaziland.

    abstract::Although no full-scale survey of the prevalence of bilharziasis in Swaziland has been carried out, examinations of schoolchildren have revealed S. mansoni infection rates of up to 49% in some areas, and S. haematobium infection rates ranging from 3% to 75%. The highest prevalence occurs in areas where there are thickl...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: PITCHFORD RJ

    更新日期:1958-01-01 00:00:00

  • National influenza experience in the USA, 1968-69.

    abstract::An extensive outbreak of Hong Kong influenza occurred in the USA during the autumn and early winter of 1968-69. Introduction and seeding of the virus occurred in September and early October as individuals returned from the Far East. Civilian outbreaks did not develop until late October and November. By 28 December, al...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Sharrar RG

    更新日期:1969-01-01 00:00:00

  • A controlled field trial of the effectiveness of phenol and alcohol typhoid vaccines: Final report.

    abstract::In order to determine the effectiveness of anti-typhoid vaccines in man a controlled field trial, the first of its kind, was organized in 1954-60 in the town and district of Osijek, Yugoslavia. Heat-killed, phenol-preserved and alcohol-killed, alcohol-preserved anti-typhoid monovaccines were used, with phenolized dyse...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Yugoslav Typhoid Commission.

    更新日期:1962-01-01 00:00:00

  • Using mortuary statistics in the development of an injury surveillance system in Ghana.

    abstract:OBJECTIVE:To develop, in a mortuary setting, a pilot programme for improving the accuracy of records of deaths caused by injury. METHODS:The recording of injury-related deaths was upgraded at the mortuary of the Komfo Anokye Teaching Hospital, Kumasi, Ghana, in 1996 through the creation of a prospectively gathered dat...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: London J,Mock C,Abantanga FA,Quansah RE,Boateng KA

    更新日期:2002-01-01 00:00:00

  • An estimate of the frequency of diagnostic X-ray examinations in Kenya in 1986.

    abstract::The frequency of radiological examinations in a country is an important indicator of the general scope of such services in that setting. Frequency data obtained from surveys provide health planners with valuable information that can form an objective basis for resource allocation. Also, from the point of view of radia...

    journal_title:Bulletin of the World Health Organization

    pub_type: 杂志文章

    doi:

    authors: Tole NM

    更新日期:1989-01-01 00:00:00