Abstract:
:Aralin from Aralia elata is a newly identified type II ribosome- inactivating protein, which preferentially induces apoptosis in cancer cells. In this study, we identified that the aralin receptor is a 110-kDa high-density lipoprotein-binding protein (HDLBP), which functions as a HDL receptor. The sensitivities of tumor cell lines to aralin were dependent on the expression levels of the 110-kDa HDLBP and its forced expression in aralin-resistant Huh7 cells conferred aralin sensitivity. HDLBP-knockdown HeLa cells showed a significant aralin resistance in vitro and in vivo. Conversely, ectopic expression of the 150-kDa HDLBP resulted in increased aralin sensitivity in vivo, accompanying enhanced expression of the 110-kDa HDLBP. Thus, these results showed that the 110-kDa HDLBP in lipid rafts acted as an aralin receptor and that its expression levels determined aralin sensitivity, suggesting that aralin could be a promising anticancer drug for HDLBP-overexpressing tumors.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Otsuka H,Gotoh Y,Komeno T,Ono T,Kawasaki Y,Iida N,Shibagaki Y,Hattori S,Tomatsu M,Akiyama H,Tashiro Fdoi
10.1016/j.bbrc.2014.09.067subject
Has Abstractpub_date
2014-10-10 00:00:00pages
117-23issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(14)01693-3journal_volume
453pub_type
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