Abstract:
:Evidence is provided for the abilities of endogenous 6-(Arg or Lys)-opioid peptides to interact with kappa-receptors as agonists. Dynorphin-(1-17) and -(1-8), alpha- and beta-neo-endorphin, [Met5]-enkephalin-Arg6-Phe7 and des acetyl salmon endorphin I significantly inhibited the electrically-evoked contractions of rabbit vas deferens which had been shown to contain kappa-receptors exclusively, indicating that endogenous 6-(Arg or Lys)-opioid peptides could act on kappa-receptors as agonists. Additionally, the inhibition of contractions of rabbit vas deferens by 6-(Arg or Lys)-opioid peptides was antagonized more effectively by Mr 2266 which had a high affinity to both mu- and kappa-receptors, than naloxone which had a high affinity only to mu-receptors. This also suggested that 6-(Arg or Lys)-opioid peptides acted as kappa-receptor agonists. The rank order of the inhibitory potency of 6-(Arg or Lys)-opioid peptides against contractions of rabbit vas deferens was as follows: dynorphin-(1-17) greater than alpha-neo-endorphin greater than beta-neo-endorphin .=. dynorphin-(1-8) greater than des acetyl salmon endorphin I greater than [Met5]-enkephalin-Arg6-Phe7. Since other endogenous opioid peptides such as [Met5]- and [Leu5]-enkephalin and beta-endorphin have been shown not to act on kappa-receptors as agonist, data in the present study suggest that endogenous opioid peptides can be classified into two groups in terms of an ability to interact with kappa-receptors as an agonist.
journal_name
Life Scijournal_title
Life sciencesauthors
Oka T,Negishi Kdoi
10.1016/0024-3205(82)90191-6subject
Has Abstractpub_date
1982-10-18 00:00:00pages
1707-10issue
16-17eissn
0024-3205issn
1879-0631pii
0024-3205(82)90191-6journal_volume
31pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Extracts of plasma obtained from adult humans before and immediately after physical stress were subjected to high pressure liquid chromatography (HPLC); extracts or HPLC fractions, resp., were analysed for the presence of human beta-endorphin (beta H-endorphin) or fragments thereof by several radioimmunoassays using a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90451-4
更新日期:1983-01-01 00:00:00
abstract::As a step in the purification and characterization of the glucose transporter from rat skeletal muscle, we have reconstituted glucose transport activity in liposomes. Plasma membranes were prepared from skeletal muscle which display D-glucose reversible binding of cytochalasin B (10 pmol sites/mg protein; KD = 0.3 mic...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90503-0
更新日期:1987-06-15 00:00:00
abstract::In this study, we have identified the E-NTPDase family members and ecto-5'-nucleotidase/CD73 in rat heart left ventricle. Moreover, we characterize the biochemical properties and enzyme activities from synaptosomes of the nerve terminal endings of heart left ventricle. We observe divalent cation-dependent enzymes that...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.12.003
更新日期:2008-02-27 00:00:00
abstract::Previous studies from this laboratory have shown that rats with experimental cirrhosis of the liver induced by the combined administration of oral phenobarbital and inhaled carbon tetrachloride show an hyperdynamic status with enhanced cardiac output (CO), and decreased mean arterial pressure (MAP) and peripheral vasc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90531-x
更新日期:1986-07-21 00:00:00
abstract:AIMS:Lung cancer represents as the most prevalent tumor globally, among which non-small cell lung cancer (NSCLC) takes up for more than 80%. Lysine demethylase 4A (KDM4A) was dysregulated in NSCLC, but its role on the tumorigenesis in NSCLC cells and the mechanisms of actions are unclear. In this work, we evaluated its...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118508
更新日期:2020-12-01 00:00:00
abstract::Involvement of cannabinoid CB2 receptors in the IgE-mediated cutaneous reaction was investigated. Epicutaneous challenge with 2,4-dinitrofluorobenzene caused a triphasic swelling in the ear of BALB/c and C57BL/6 mice passively sensitized with anti-dinitrophenol IgE. Peak responses of the ear swelling appeared at 1 h, ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.09.026
更新日期:2007-01-09 00:00:00
abstract::Endothelins (ET) are 21-amino acid peptides that bind to membrane receptors to initiate a wide range of pathophysiological effects. PD-156707, L-749329, Ro-470203, and A-127722 are potent non-peptide ET receptor antagonists developed recently. When tested in human and rat plasma, both ET-1 and -3 and the four aforemen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00168-3
更新日期:1996-01-01 00:00:00
abstract::Studies have reported that taraxasterol (TAR) is effective in the treatment of immune liver injury and alcoholic liver injury. The mechanism of action is mainly related to the inhibition of inflammation. To determine the key molecular mechanisms for the effect of TAR on alleviating ethanol and high-fat diet-induced li...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118546
更新日期:2020-12-01 00:00:00
abstract::Aluminum (Al) is thought to be a risk factor for neurodegenerative disorders, but the molecular mechanism has been not clarified yet. In this study, we examined how a transport system handled transport of Al citrate, the major Al species in brain, and effect of Al citrate treatment on expression of the transporter and...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.12.048
更新日期:2006-05-30 00:00:00
abstract::The cellular electrophysiology of left ventricular preparations from guinea pig hearts was studied 1 hour, 24 hours, and 4-6 weeks after myocardial infarction produced by 6-8 single ties of the distal left coronary artery system or after sham operation. Microelectrode recordings were used to monitor cells from the end...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90343-1
更新日期:1985-06-24 00:00:00
abstract::Herba Epimedii is an important medicinal plant which has been used in various traditional Chinese formulations for thousands of years as well as in modern proprietary traditional Chinese medicine products. It has extensive clinical indications, especially for the treatment of sexual dysfunction and osteoporosis. There...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2015.01.006
更新日期:2015-04-01 00:00:00
abstract::The mechanism for the development of insulin resistance in normal pregnancy is complex and is associated with serum levels of sex hormones. However, the influence of these hormones on the early steps of insulin action has not been extensively studied, although the potentially beneficial effect of estradiol on glucose ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01489-3
更新日期:2002-02-22 00:00:00
abstract::Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val, melanin concentrating hormone (MCH), is a cyclic hormone possessing both MCH-like (melanin granule aggregating effect) and melanocyte stimulating hormone (MSH)-like (melanin granule dispersing effect) activities. Nine ring-contracted analogues were s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90460-8
更新日期:1989-01-01 00:00:00
abstract::Intracellular calcium plays an important role on the pathogenesis of hypoxia-induced cellular injury. Calbindin-D(28k), a cytosolic vitamin D-dependent calcium binding protein, can serve as a buffer to limit a surge in intracellular Ca2+ concentration ([Ca2+]i) induced by various stimulations. To evaluate the possible...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01710-1
更新日期:2002-06-21 00:00:00
abstract:AIMS:To investigate how Placental Mesenchymal Stem Cells (P-MSCs) would adapt themselves and survive under hypoxic conditions which are prevalent in most injury sites. MAIN METHODS:P-MSCs were isolated from term placenta and characterised under normoxia and hypoxia (2-2.5% O2). Cells were examined for morphology and s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.06.016
更新日期:2017-08-01 00:00:00
abstract::Acute myocardial infarction is associated with high rates of morbidity and mortality and can cause irreversible myocardial damage. Timely reperfusion is critical to limit infarct size and salvage the ischemic myocardium. However, reperfusion may exacerbate lethal tissue injury, a phenomenon known as myocardial ischemi...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118628
更新日期:2021-01-01 00:00:00
abstract::Hyaluronan (HA) is a multifunctional high molecular weight polysaccharide found throughout the animal kingdom, especially in the extracellular matrix (ECM) of soft connective tissues. HA is thought to participate in many biological processes, and its level is markedly elevated during embryogenesis, cell migration, wou...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2007.02.037
更新日期:2007-05-01 00:00:00
abstract::The objective of the present study was to investigate the roles of protein kinase A and/or C in agonist-induced beta adrenoceptor activation in intact human lymphocytes. LYmphocytes from healthy subjects were incubated with isoproterenol and phosphodiesterase inhibitor (IBMX, 1.0 mM) after 20 minutes of preincubation ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90584-e
更新日期:1990-01-01 00:00:00
abstract::The effects of isolation and water scheduling on mitogen induced lymphocyte proliferation were investigated. Isolated rats were animals which had been raised in group-housed conditions and then transferred to individual cages with ad lib access to water for a 1 or 2 week period. Water scheduled rats were maintained in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90472-9
更新日期:1988-01-01 00:00:00
abstract::The flavonoid quercetin exhibited a biphasic effect on calcium and phospholipid-dependent protein kinase (protein kinase C) activity from rat brain and pig thyroid. At a low concentration (10(-7) M) quercetin stimulated the enzyme activity whereas at higher concentrations quercetin was inhibitory. By contrast the synt...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90450-5
更新日期:1989-01-01 00:00:00
abstract::Recent studies suggest lipopolysaccharide (LPS) mediated cell death as underlying mechanism of hyporesponsiveness and dysfunction of macrophages in the late phase of septic shock. In the present study LPS (0.001 - 30 microg/ml) caused a concentration-dependent toxicity in the macrophage cell line (J774.1A) within 24 h...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00031-9
更新日期:1998-01-01 00:00:00
abstract::Binding studies conducted on membrane preparation from human platelets using (3H) Ro5-4864 and (3H) diazepam showed specific and saturable binding. Scatchard analysis revealed a single class of binding sites with KD = 10.8 +/- 0.9 nM and Bmax = 775 +/- 105 fmol/mg protein for (3H) Ro5-4864 and KD = 10.5 +/- 1.1 nM and...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90556-3
更新日期:1984-11-12 00:00:00
abstract::Since the late 1960's, radioenzymatic assays have gradually come to replace the less sensitive and less specific spectrofluorometric and bioassay procedures previously used to determine many of the neurotransmitters. These assays provide the means to measure picogram quantities of most of these substances, and have en...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90185-8
更新日期:1987-08-17 00:00:00
abstract::Convulsive seizures are a potential side effect of antidepressant drug treatment and can be produced by all classes of antidepressants. It is also known that some convulsant and anticonvulsant drug actions are mediated by the GABA-receptor chloride-ionophore complex. Drugs acting at this complex appear to induce convu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90107-5
更新日期:1988-01-01 00:00:00
abstract::We evaluated the effect of several classes of calmodulin inhibitors on the activity of calmodulin prepared from C6 astrocytoma cells and studied the activity of these drugs as inhibitors of the growth of C6 cells in tissue culture. There was a good correlation between the activity of the drugs as inhibitors of calmodu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90120-1
更新日期:1985-01-28 00:00:00
abstract::Twice daily injection of caffeine (30 mg/kg) for 3-1/2 days shifted the caffeine stimulus generalization curve to the right by 3-fold in rats trained to discriminate saline from 30 mg/kg of caffeine, and by 4-fold in rats trained to discriminate saline from 10 mg/kg of caffeine. The latter group was also tested for cr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90140-8
更新日期:1987-01-26 00:00:00
abstract::Morphine and the enkephalin-like peptide FK 33-824 given i.p. to rats potently inhibit gastrointestinal transit of a charcoal test meal, the doses inhibiting transit by 50% being respectively about 10 and 1 microgram/kg. These intestinal effects are opiate-specific, locally elicited and presumably involve the same act...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90359-9
更新日期:1982-09-20 00:00:00
abstract::The effect of triiodothyronine (T3) on Na+,K(+)-ATPase activity of K562 human erythroleukemic cell was studied to understand why the erythrocyte sodium pump activity is decreased in hyperthyroidism. Na+,K(+)-ATPase activity of K562 cell lysates was assayed by measuring the release of inorganic phosphate (Pi) from ATP....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90107-z
更新日期:1992-01-01 00:00:00
abstract::Lymphocyte apoptosis occurs in response to stressors such as thermal injury, trauma, sepsis, and surgery. This study evaluated the effect of a single bout of physical exercise stress on the induction of apoptosis in murine thymocytes and splenic lymphocytes. Female C57BL/6 mice, treadmill exercised at a submaximal int...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00551-7
更新日期:1999-01-01 00:00:00
abstract::This investigation was designed to determine whether St. John's wort (SJW)(435 mg/kg/d), a readily available antidepressant, or its purported active constituents hypericin (1 mg/kg/d) and hyperforin (10 mg/kg/d) were able to induce various hepatic cytochrome P450 (CYP450) isoforms. SJW, hypericin and hyperforin were a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01499-0
更新日期:2002-02-01 00:00:00